Karyotype of the three Astyanax scabripinnis populations (a, b Lavrinha Farm Stream – LSF); (c, d Lake of Pedalinho – LP); (e, f Ribeirão das Perdizes – RP). In a, c, e are visualized signals of 18S (red) and 5S (green) with rDNA probes and in b, d, f H3 (red) and H4 (green) with histones probes, after double Fluorescence in situ Hybridization (FISH), respectively. Scales bar: 10 µm.