Microdissection of H. japonicus sex chromosomes at meiotic diakinesis-metaphase I stage. a Selection of sex chromosomes (Y1-X-Y2 trivalent formation indicated by arrow) b Cutting out of the sex chromosomes c The gel electrophoresis of the microdissected sex chromosomes DOP-PCR product: 1 – negative control, 2 – 100 bp DNA ladder, 3 - DOP-PCR d The gel electrophoresis after PCR with the male sex specific marker on different DNA temfigs: 1 – 100 bp DNA ladder, 2 – DOP-PCR product from sex chromosomes, 3 – DOP-PCR product from autosomes, 4 – DNA of male plant, 5 – DNA of female plant.

 
 
  Part of: Yakovin N, Divashuk M, Razumova O, Siloviev A, Karlov G (2014) Use of laser microdissection for the construction of Humulus japonicus Siebold et Zuccarini, 1846 (Cannabaceae) sex chromosome-specific DNA library and cytogenetics analysis. Comparative Cytogenetics 8(4): 323-336. https://doi.org/10.3897/CompCytogen.v8i4.8473