Simultaneous visualization of different genomes (J, JSt and St) in a Thinopyrum intermedium × Thinopyrum ponticum synthetic hybrid (Poaceae) and in its parental species by multicolour genomic in situ hybridization (mcGISH)

Results of multicolour genomic in situ hybridization on Thinopyrum intermedium. a Karyotype of a complete cell using Thinopyrum bessarabicum (J, green) and Pseudoroegneria spicata (St, red) genomic DNA as probes. Chromosome with satellite is indicated with arrow b Karyogram of Thinopyrum intermedium chromosomes. Top row: J chromosomes; middle row: JSt chromosomes with the St pericentromeric region; bottom row: St chromosomes. Bar = 10 μm.