FISH on the chromosomes of R. wichurana. AFISH with Arabidopsis-type telomere probe (red) and 45S (green) under hybridization at 37°C BFISH with Arabidopsis-type telomere probe under the low hybridization stringency condition (23-25°C). Arrows indicate the major ITRs on chromosome 5 and arrowheads show the ITRs which are visible under the low hybridization stringency condition C The same metaphase as in 1B rehybridized with 5S rDNA under the common hybridization stringency (37°C). Arrows indicate the 5S rDNA signals. Sacale bar: 5 µm.