Mitotic chromosomes of A. porphyrocalyx. a–b Feulgen technique c double fluorescent in situ hybridization (FISH). a Metaphase displaying 2n=18, the starts indicate satellites and the arrows indicate the pair of “A chromosomes” b Prometaphase showing the pair of “A” chromosomes indicated by arrows c The yellow-green and red signals correspond to the 5S and 45S rDNA loci, and the white correspond to the heterochromatin bands C-DAPI+ after FISH. The arrows indicate the pair of “A chromosomes”. Bar = 3 µm.