Male karyotype of Mapuchea chilensis. a metaphase I, n = 8AA + XY b diakinesis, largest bivalent with two chiasmata c diplotene, 5 bivalents with two chiasmata each d metaphase II with the largest chromosome in the center of a ring formed by autosomes. Note chromatin associations between non-homological chromosomes e metaphase I showing associations between bivalents f anaphase II with lagging chromosomes (arrows) g diplotene (C-banding) showing terminal C-bands in chromosomes h early prophase (NOR-banding) showing argyrophilic granules associated with autosomes i–k diplotenes (i, j) and mitotic metaphase (k) after FISH with rDNA-probe (green signals) and (TTAGG)n telomeric probe (red signals). rDNA sites are located on a medium-sized pair of autosomes. Bar = 10µm.