Simultaneous visualization of different genomes (J, JSt and St) in a Thinopyrum intermedium × Thinopyrum ponticum synthetic hybrid (Poaceae) and in its parental species by multicolour genomic in situ hybridization (mcGISH)

Multicolour genomic in situ hybridization on Thinopyrum ponticum. a Karyotype of Th. ponticum (accession VIR-44486) carrying 40 J and 30 JStchromosomes, using Thinopyrum bessarabicum (J, green) and Pseudoroegneria spicata (St, red) genomic DNA as probes b 38 J and 32 JStchromosomes identified in Th. ponticum (accession D-3494) c JS chromosomes with different lengths of St DNA in the centromeric region. JSt chromosomes were marked with asterisks. Bar = 10 μm.