Simultaneous visualization of different genomes (J, JSt and St) in a Thinopyrum intermedium × Thinopyrum ponticum synthetic hybrid (Poaceae) and in its parental species by multicolour genomic in situ hybridization (mcGISH)

Multicolor genomic in situ hybridization pattern of Agropyron glael and the wheat (Mv9kr1 genotype)/A. glael F1 hybrid. a Karyotype of a partial cell of A. glael using Thinopyrum bessarabicum (J, green) and Pseudoroegneria spicata (St, red) DNA probes. Translocation between J and St chromosomes were marked with arrows b Karyotype of a complete cell of wheat (Mv9kr1 genotype)/A. glael F1 hybrid using Th. bessarabicum (J, green) and Ps. spicata (St, red) genomic DNA simultaneously as probes and wheat genomic DNA as block simultaneously c Karyogram of A.glael chromosomes present in the wheat/A.glael F1 hybrid. Nine A. glael chromosomes with hybridization patterns different to their parental species are marked with asterisks. Bar = 10 μm.