Meiotic cell of C. gomesi in pachytene stage with three supernumerary chromosomes. a Synaptonemal complex protein identified anti-SYCP3 (green) and B chromosome probe (red). Note the positive hybridization of B paint probe on two bivalents and one univalent (ZW, 2B and 1B, respectively) b Synaptonemal complex protein identified with anti-SYCP3 counterstained with DAPI (blue), evidencing ZW bivalent and the meiotic formations of B chromosomes; chromatin regions associated with transcriptional activities identified with anti-H3K4m (red) and DAPI consterstaining (blue) evidencing the ZW bivalent and the meiotic formations of B chromosomes. Bar = 10 μm.