High resolution physical mapping of ITR on R. wichurana pachytene chromosomes. FISH with the Arabidopsis-type telomere repeat probe (red) and 45S (green). Merged (A) and the DAPI gray scale (B) pictures are shown. FISH was performed under the low hybridization stringency condition. Dotted lines show the regions that were digitally enlarged (A’ and B’). Scales bar: 5 µm.