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Karyotypes of ten sections of genus Trigonella Linnaeus, 1753 (Fabaceae) from Turkey were investigated. Somatic chromosome numbers of examined species were determined as 2n=14 and 16. The karyotype analyses of the species were carried out and somatic chromosome numbers of Trigonella plicata Boiss., 1872, Trigonella brachycarpa (Fisch.) Moris, 1833, Trigonella rostrata Boiss., 1872, Trigonella lunata Boiss., 1843, Trigonella isthmocarpa Boiss. et Balansa 1856, Trigonella rhytidocarpa Boiss. et Balansa, 1859, Trigonella spicata Sibth. et Sm., 1813, Trigonella cephalotes Boiss. et Balansa 1856, Trigonella capitata Boiss., 1843 and Trigonella gladiata Steven, 1808 were reported for the first time. Two pairs of satellite metaphase chromosomes were observed in Trigonella cariensis Boiss., 1843 and one pair in Trigonella lunata.Moreover, 2 B-chromosomes were found only in Trigonella procumbens Rchb., 1830 among all studied species. The aims of this study are to provide karyological data for a significant pool of the taxa, to show differences among them in the number, size and morphology of somatic chromosomes, to verify previous reports or represent numbers which are different from those cited previously.
omatic chromosome, Turkey, Fabaceae, karyotype
Trigonella L., 1753 (Fabaceae) includes about 135 species worldwide, and most of the species are distributed in dry regions around Mediterranean, West Asia, Europe, North and South Africa, North America, and with only two species being present in South Australia (
According to the literature, some studies conducted on the karyology of the Trigonella include approximately hundred species (
In the present work we carried out a karyological study on 19 species of Trigonella, belonging to ten sections, collected from different regions of Turkey (
Seedlings were collected between the years of 2002 and 2005 from natural habitats in different localities (Table 1). For karyotype analyses, root tips were obtained from seeds germinated in humidified Petri dishes at room temperature. Root tips were pretreated with α-monobromonaphthalene at 4 °C for 16 h and fixed in Carnoy’s fixative for 24 h at 4 °C. Before staining, the material was hydrolyzed with 1N HCl for 13–15 minutes at room temperature. The chromosomes were stained with 2% aceto orcein and mounted in 45% acetic acid. Permanent slides were made by using the standard liquid nitrogen method and then examined under Olympus BX50 Photomicroscope using an oil immersion objective (100 X). Photographs were taken with the same microscope. Karyotype analyses were made by the use of an Image Analysis System (Bs200Pro).
ResultsThis study was carried out to analyse the karyotypes of 19 species, eight of which are endemic to Turkey, belonging to ten sections of the genus Trigonella in Turkey. These sections are: Samaroideae Boiss., Pectinatae Boiss., Lunatae Boiss., Falcatulae Boiss., Reflexae (Širj.) Vass., Isthmocarpae Boiss., Uncinatae Boiss., Capitatae Boiss., Biebersteinianae (Širj.), and Foenum-graecum Ser. Cytological results obtained from our study are arranged based on the order in the Flora of Turkey (Huber-Morath 1969).
Mitotic metaphase chromosomes of Trigonella species A Trigonella cretica B Trigonella plicata C Trigonella brachycarpa D Trigonella rostrata E The satellite (arrow) of chromosomes Trigonella lunata F Trigonella corniculata; no: 4616 G Trigonella corniculata no: 3391 H Trigonella spinosa I Trigonella monspeliaca no: 3358 J Trigonella monspeliaca no: 3327 K Trigonella isthmocarpa L Trigonella rhytidocarpa.Scale bar = 10 µm.
Mitotic metaphase chromosomes of Trigonella species M Trigonella spicata N Trigonella cephalotes O B chromosomes (arrow) Trigonella procumbens P Trigonella capitata R Trigonella coerulescens no: 3587 S Trigonella coerulescens no: 3659 T Trigonella gladiata U The satellite (arrow) of chromosomes Trigonella cariensis no: 3332 V Trigonella cariensis no: 4620; W Trigonella foenum-graecum X Trigonella macrorrhyncha.Scale bar = 10 µm.
Idiograms of Trigonella species (Trigonella corniculata no: 4616 and 3391; Trigonella monspeliaca no: 3358 and no: 3327; (Trigonella coerulescens no: 3587and3659; Trigonella cariensis no: 3332 and4620).
Idiograms of Trigonella species (Trigonella corniculata no: 4616 and 3391; Trigonella monspeliaca no: 3358 and no: 3327; (Trigonella coerulescens no: 3587and3659; Trigonella cariensis no: 3332 and4620).
Localities, collector name and numbers of studied Trigonella species.
Taxa | Locality | Collector name and number |
---|---|---|
Trigonella cretica | Burdur: Gölhisar-Dirmil 4. km, 30.05.2002, 900–950 m, conserved area | H.Akan 3480, M. Ekici |
Trigonella plicata | Konya: Hadim-Konya 10. km, 17.07.2002, 1350 m, roadside | H.Akan 3789, Z.Aytaç, M.Ekici |
Trigonella brachycarpa | Konya: Hadim-Konya 10. km, 17.07.2002, 1400 m, roadside | H.Akan 3786, Z.Aytaç, M.Ekici |
Trigonella rostrata | Konya: East of Hadim-Karaman, 17.07.2002, 1250 m, roadside | H.Akan 3805, Z.Aytaç, M.Ekici |
Trigonella lunata | Adana: Pozantı-Çiftehan 8.km, 08.06.2003, 852 m, stony places | H.Akan 4687, M.Ekici |
Trigonella corniculata | Izmir: Bayraklı, 26.05.2002, 20–50m, meadowsMuğla: Bodrum castle, 25.05.2002, 10 m, meadows | H.Akan 4616, M.EkiciH.Akan 3391, M.Ekici |
Trigonella spinosa | Muğla: Marmaris-Datça 50. km, 06.05.2005, 5–10 m, maqius | H.Akan 5655, M.Ekici |
Trigonella monspeliaca | Muğla: Dalaman, Ortaca district, 24.5.2002, 20 m, stony placesAntalya: Exit of Antalya-Hafızpaşa, 23.05.2002, 750 m, maqius | H.Akan 3358, M.EkiciH.Akan 3327, M.Ekici |
Trigonella isthmocarpa | Aksaray: 28 km E of Aksaray, Hasan mountain foots, 08.06.2003, 1145 m | H.Akan 4695, M.Ekici |
Trigonella rhytidocarpa | Niğde: Ulukışla-Pozantı 5. km, 16.07.2002, 1300 m, roadside | H.Akan 3778, M.Ekici |
Trigonella spicata | Antalya-Bucak 20.km, Pınarbaşı district, 23.05.2002, 220–250 m, opening of Quercus coccifera | H.Akan 3319, M.Ekici, Karavelioğulları |
Trigonella cephalotes | Aydın: Dilek Peninsula, National park, Aydınlık cove, 26.02.2002, 70 m | H.Akan 3398, M.Ekici |
Trigonella procumbens | Konya: Akşehir-Gelendost 1–2.km, 22.06.2003, 1110 m, roadside of vineyards | H.Akan 4760, M.Ekici |
Trigonella capitata | Denizli: Pamukkale, 22.06.2003, 1550 m, opening of damaged forest | H.Akan 4767, M.Ekici |
Trigonella coerulescens | Konya: Ereğli-Karapınar 20–25km, 01.06.2002 1000 m, steppeKayseri: Kayseri, Hisarcık, Erciyes mountain road, 08.07.2002, 1550 m | H.Akan 3587 Z.Aytaç, M.EkiciH.Akan 3659, M.Ekici |
Trigonella gladiata | Karaman: Pınarbası-Kızılkaya 2 km, 28.06.2005, 1200 m, opening of Oak | H.Akan 5743, M.Ekici |
Trigonella cariensis | Izmir: Ayrancılar-Izmir 6.km, 25.05.2003, 100 m, foots of maqiusAntalya: Antalya-Hafızpaşa-Bucak 5.km, 23.05.2002, 775 m | H.Akan 4620, M.EkiciH.Akan 3332, M.Ekici |
Trigonella foenum-graecum | Adana: Ceyhan-Adana 13.km., 20.05.02, 120 m, fields | H.Akan 3274 |
Trigonella macrorrhyncha | Içel: Tarsus-Çamlıyayla road, 30.km, 18.05.2003, 850 m, steppe | H.Akan 4354, M.Ekici |
Trigonella cretica (L.) Boiss., 1872, 2n=16 (Fig. 1, A). All chromosome pairs at the somatic metaphase phase were observed metacentric. The chromosome length ranges between 0.52 and 1.01 μm. The total haploid chromosome length was measured as 5.88 μm.
Section: PectinataeTrigonella plicata (Boiss. et Bal.) Boiss., 1872, 2n=14 (Fig. 1, B). The species has metacentric chromosome pairs at somatic metaphase. The chromosome length ranges from 0.84 to 1.34 μm with the total haploid chromosome length of 7.12 μm. This species is endemic of Turkey.
Section: LunataeTrigonella brachycarpa (Fisch.) Moris, 1833, 2n=16 (Fig. 1, C). Trigonella rostrata (Boiss. & Bal.) Boiss., 1872, 2n=14 (Fig. 1, D). Trigonella lunata Boiss., 1843, 2n=16 (Fig. 1, E). All of the species of this section examined have metacentric chromosome pairs at somatic metaphase plates. For Trigonella brachycarpa, chromosome length ranges from 0.94 to 1.36 μm having total chromosome length of 8.85 μm. Trigonella rostrata being endemic to Turkey was observed having chromosomes whose lengths range between0.81 and 1.22 μm. In this species, the total haploid chromosome length was measured as 7.07 μm. For the species Trigonella lunata, the chromosome length was measured between 2.85 and 3.92 μm with the total haploid chromosome length of 26.44 μm. In addition, this species has a pair of satellite metaphase chromosomes.
Section: FalcatulaeTrigonella corniculata L., 1759 from the specimen collected from İzmir province; 2n=16 (Fig. 1, F) and from the specimen collected from Muğla province; 2n=16 (Fig. 1, G). At somatic metaphase, seven pairs of metacentric and one pair of submetacentric chromosomes were observed in the former while four pairs of metacentric and four pairs of submetacenric chromosomes were detected in the latter. The specimen collected from İzmir province has chromosomes ranging between 0.61 and 0.88 μm, and the total haploid chromosome length for this specimen was measured as 5.71 μm. In the other specimen which was collected from Muğla province, the chromosome length ranges between 1.54 and 2.57 μm with the total haploid chromosome length of 17.58 μm. Trigonella spinosa L., 1753, 2n=16 (Fig. 1, H). At somatic metaphase, two pairs of metacentric and six pairs of submetacentric chromosomes were observed. For this species, the chromosome lengths range from 1.49 to 2.76 μm with the total haploid chromosome length of 16.76 μm.
Section: ReflexaeTrigonella monspeliaca L. 1753 from the specimen collected from Muğla province; 2n=16 (Fig. 1, I) while the specimen collected from Antalya province; 2n=16 (Fig. 1, J). In the former, all chromosome pairs at the somatic metaphase were observed to be metacentric, and the chromosome length ranges between 0.66 and 1.18 μm with the total haploid chromosome length of 6.81 μm. In the latter specimen, five pairs of metacentric and three pairs of metacentric chromosomes were observed at somatic metaphase. The chromosome length ranges between 0.74 and 1.00 μm. The total haploid chromosome length was measured as 7.06 μm.
Section: IsthmocarpaeTrigonella isthmocarpa Boiss. et Bal., 1856, 2n=16 (Fig. 1, K) and Trigonella rhytidocarpa Boiss. & Bal. 2n=16 (Fig. 1, L). Endemic to Turkey, both species have metacentric chromosome pairs at the somatic metaphase. For the species of Trigonella isthmocarpa, the chromosome length ranges between 0.57 and 1.10 μm. The total haploid chromosome length was measured as 6.53 μm. For Trigonella rhytidocarpa, the chromosome length ranges between 0.85 and 1.40 μm with the total haploid chromosome length of 8.47 μm.
Section: UncinataeTrigonella spicata Sibth. et Sm., 1813, 2n=16 (Fig. 1, M) and Trigonella cephalotes Boiss. et Bal., 1856, 2n=16 (Fig. 1, N). At somatic metaphase, five pairs of metacentric and three pairs of submetacentric chromosomes were observed for both species. The chromosome length of Trigonella spicata ranges between 1.00 and 1.50 μm with the total haploid chromosome length of 10.36 μm. For Trigonella cephalotes, the chromosome length ranges between 0.43 and 0.94 μm. The total haploid chromosome length was measured as 4.49 μm. This species is endemic to Turkey.
Section: CapitataeTrigonella procumbens (Besser) Reichp., 1830, 2n=16 (Fig. 1, O) and Trigonella capitata Boiss., 1843, 2n=16 (Fig. 1, P). Both species have metacentric chromosome pairs at the somatic metaphase. Endemic to Turkey, Trigonella procumbens have chromosomes ranging from 0.82 to 1.32 μm with the total haploid chromosome length of 7.75 μm. Moreover, two B chromosomes are observed in this species. The other species of the section examined, Trigonella capitata, have chromosomes ranging from 0.96 to 1.30 μm. The total haploid chromosome length was measured as 9.33 μm. This species is also endemic to Turkey.
Section: BiebersteinianaeTrigonella coerulescens (Bieb.) Hal., 1901, in the specimen collected from Konya province; 2n=16 (Fig. 1, R) and in the specimen collected from Kayseri province; 2n=16 (Fig. 1, S). All chromosome pairs at the somatic metaphase phase were observed to be metacentric for both specimens. The chromosome length ranges between 1.38 and 1.91 μm for the former while that of the latter ranges between 0.91 and 1.32 μm. The total haploid chromosome length was measured as 12.91 μm in the specimen collected from Konya while that of the other specimen was measured as 9.06 μm.
Section: Foenum-graecumTrigonella gladiata Stev. Fischer, 1808, 2n=16 (Fig. 1, T). All chromosome pairs at the somatic metaphase phase were observed to be metacentric. The chromosome length ranges between 2.03 and 4.72 μm. The total haploid chromosome length was measured as 27.70 μm. Trigonella cariensis Boiss. In the specimen collected from İzmir province; 2n=16 (Fig. 1, U), and in the specimen collected from Antalya province; 2n=16 (Fig. 1, V). Both specimens have four pairs of metacentric and four pairs of submetacentric chromosomes at somatic metaphase. Of the two specimens, the former has chromosomes whose lengths vary from 2.66 to 3.94 μm with the total haploid chromosome length of 25.28 μm. For the latter, the chromosome length ranges between 3.93 and 6.28 μm, and the total haploid chromosome length was measured as 39.78 μm. In addition, one pair of satellite metaphase chromosomes has been detected in the specimen from Antalya province. Trigonella foenum-graecum L., 1753, 2n=16 (Fig. 1, W), and Trigonella macrorrhyncha Boiss., 1843, 2n=16 (Fig. 1, X). At somatic metaphase, two pairs of metacentric and six pairs of submetacentric chromosomes were observed for both species. For Trigonella foenum-graecum, the chromosome length ranges between 3.03 and 4.84 μm, and the total haploid chromosome length was measured as 30.23 μm. Endemic to Turkey, Trigonella macrorrhyncha has chromosomes varying from 2.23 to 4.40 μm. In this species, the total haploid chromosome length is 25.67 μm. Idiograms of each species were arranged in order of decreasing length (Fig. 2). The total haploid chromosome lengths were given in Table 2, and the information of the 19 species studied were presented in Table 3.
Chromosome comparison in the examined species of Trigonella (AR: arm ratio; CI: centromeric index; THC: total length of haploid complement; M: metacentric; SM: submetacentric; *one pair of satellites is shown in the chromosome pairs are marked with an asterisk).
Sections and species | Chromosomenumbers (2n=2x) | Chromosomesizes (µm) | AR | CI | THL(µm) | M | SM |
---|---|---|---|---|---|---|---|
Section Samaroideae Trigonella cretica |
16 | 0.52–1.01 | 1.33 | 5.40 | 5.88 | 8 | - |
Section Pectinatae Trigonella plicata |
14 | 0.84–1.34 | 1.37 | 6.06 | 7.12 | 7 | - |
Section Lunatae Trigonella brachycarpa Trigonella rostrata Trigonella lunata* |
16 14 16 |
0.94–1.36 0.81–1.22 2.85–3.92 |
1.26 1.17 1.46 |
5.58 6.56 5.12 |
8.85 7.072 6.44 |
878 | --- |
Section Falcatulae Trigonella corniculata Trigonella corniculata Trigonella spinosa |
16 16 16 |
0.61–0.88 1.54–2.57 1.49–2.76 |
1.46 1.78 1.99 |
5.15 4.59 4.25 |
5.711 7.581 6.76 |
742 | 146 |
Section Reflexae Trigonella monspeliaca Trigonella monspeliaca |
16 16 |
0.66–1.18 0.7 4–1.00 |
1.40 1.50 |
5.26 5.11 |
6.81 7.06 |
85 | -3 |
Section Isthmocarpae Trigonella isthmocarpa Trigonella rhytidocarpa |
16 16 |
0.57–1.10 0.85–1.40 |
1.31 1.34 |
5.47 5.37 |
6.53 8.47 |
88 | -- |
Section Uncinatae Trigonella spicata Trigonella cephalotes |
16 16 |
1.00–1.50 0.43–0.94 |
1.52 1.59 |
5.07 4.96 |
10.36 4.49 |
55 | 33 |
Section Capitatae Trigonella procumbens Trigonella capitata |
16+2B 16 |
0.82–1.32 0.96–1.30 |
1.31 1.19 |
5.43 5.68 |
7.75 9.33 |
88 | -- |
Section Biebersteinianae Trigonella coerulescens Trigonella coerulescens |
16 16 |
1.38–1.91 0.91–1.32 |
1.32 1.25 |
5.45 5.62 |
12.9 19.06 |
88 | -- |
Section Foenum-graecum Trigonella gladiata Trigonella cariensis* Trigonella cariensis Trigonella foenum-graecum Trigonella macrorrhyncha |
16 16 16 16 16 |
2.03–4.72 2.66–3.94 3.93–6.28 3.03–4.84 2.23–4.40 |
1.29 1.83 1.78 2.17 1.85 |
5.50 4.59 4.53 4.18 4.37 |
27.70 25.28 39.78 30.23 25.67 |
8 4 4 2 3 |
- 4 4 6 5 |
The information of the 19 species studied.
Section | Species | Chromosome number(2n) | Chromosome numbers reported (2n) | References |
---|---|---|---|---|
Samaroideae | Trigonella cretica | 16 | 16 | Yılmaz, 2006 |
Pectinatae | Trigonella plicata | 14 | - | - |
Lunatae | Trigonella brachycarpa | 16 | - | - |
“ | Trigonella rostrata | 14 | - | - |
“ | Trigonella lunata | 16 | - | - |
Falcatulae | Trigonella corniculata | 16 | 16 | Tutin, Heywood, 1964 |
“ | Trigonella spinosa | 16 | 16 | Bidak, Amin, 1996 |
Reflexae | Trigonella monspeliaca | 16 | 16 | Darlington, Wylie, 1955 |
Isthmocarpae | Trigonella isthmocarpa | 16 | - | - |
“ | Trigonella rhytidocarpa | 16 | - | - |
Uncinatae | Trigonella spicata | 16 | - | - |
“ | Trigonella cephalotes | 16 | - | - |
Capitatae | Trigonella procumbens | 16 + 2B | 18 | Yılmaz, 2006 |
“ | Trigonella capitata | 16 | - | - |
Biebersteinianae | Trigonella coerulescens | 16 | 16 | Yılmaz, 2006 |
Foenum-graecum | Trigonella gladiata | 16 | 16 | Bidak, Amin 1996 Darlington, Wylie, 1955 Ladizinsky, Vosa, 1986 |
“ | Trigonella cariensis | 16 | 16 | Ladizinsky, Vosa, 1986 |
“ | Trigonella foenum-graecum | 16 | 16 | Ladizinsky, Vosa 1986; Bal, 1990; Tutin, Heywood, 1964 |
“ | Trigonella macrorrhyncha | 16 | 16 | Ladizinsky, Vosa, 1986 |
In this karyological study, two different basic chromosome numbers of x=7 and x=8 were observed in the species belonging to ten sections of Trigonella.
Two different somatic chromosome numbers (2n=14 and 2n=16) were observed in studied sections. The smallest chromosome length is 0.43 μm measured in Trigonella cephalotes (section Uncinatae) while the biggest of that is 6.28 μm measured in Trigonella cariensis (section Foenum-graecum). The smallest total haploid chromosome length was measured as4.49 μm from the species of Trigonella cephalotes (section Uncinatae). Trigonella cariensis (section Foenum-graecum) has the biggest total haploid chromosome length of 39.78 μm. Trigonella rostrata (section Lunatae) has the smallest arm ratio (1.17), and Trigonella foenum-graecum (section Foenum-graecum) has the biggest (2.17). The smallest centromeric index (4.18) was measured in Trigonella foenum-graecum (section Foenum-graecum) while the biggest of that (6.56) was observed in Trigonella rostrata (section Lunatae). In this study, there is a marked difference in somatic chromosome lengths compared to other species of the section Foenum-graecum. Chromosome numbers are rather close to each other excluding several species (Lunatae, Trigonella corniculata and Trigonella spinosa) in other sections. Besides, 2B chromosome was observed in Trigonella procumbens in the section Capitatae.
Karyotype formulae of the sections of Samaroideae, Pectinatae, Lunatae, Isthmocarpae, Capitatae and Biebersteinianae are completely composed of metacentric chromosome pairs. Karyotype formulae of species in other sections are composed of metacentric and submetacentric chromosome pairs. While there is one pair of satellite metaphase chromosomes in Trigonella lunata (section Lunatae), Trigonella cariensis (section Foenum-graecum) has two pairs of satellite metaphase chromosomes. Section Samaroideae is represented by a single species (Trigonella cretica) in Turkey. The karyotype of this species has been analysed by Yılmaz (2006) reporting the karyotype formulae 2n=16 as in our study. Our results agree with one of the reports of somatic chromosome number of 2n=16 from the same locality (Yılmaz 2006).
Section Pectinatae is represented by a single species (Trigonella plicata) in Turkey. Karyotype analysis of this species has been performed by us for the first time. Section Lunatae is represented by four species (Trigonella brachycarpa, Trigonella rostrata, Trigonella lunata and Trigonella sırjaevii Hub.-Mor., 1939) in Turkey. Making a general evaluation of the section, diploid chromosome numbers were found to be different although the localities of the two types (Trigonella brachycarpa and Trigonella rostrata) in the section were similar. The diploid chromosome number is 2n=16 in Trigonella brachycarpa whereas it is 2n=14 in Trigonella rostrata. The smallest chromosome length of 0.81 μm was measured in Trigonella rostrata in this section while the biggest of that was observed in Trigonella lunata with a length of 3.92 μm. Trigonella rostrata is the species with the smallest arm length of 1.17; however, it is also the species with the biggest centromeric index (6.56). Trigonella lunata is the one with the biggest total haploid chromosome length of 26.44 μm. This length is rather different compared to the species in other sections. Besides, one pair of satellite chromosome has been observed in Trigonella lunata. Karyotype formulae of all species in the section are composed of metacentric chromosome pairs. Trigonella sırjaevii could not been studied due to its inability to be germinated.
Section Falcatulae is represented by two species (Trigonella corniculata and Trigonella spinosa) in Turkey. Trigonella corniculata was studied in two different localities (İzmir and Muğla). In Muğla case, tetraploidy was observed differing from the other. Total haploid chromosome length was measured as 5.71 μm in İzmir province while that was 17.58 μm in Muğla province. It can be stated that the distinction between the karyological values obtained from these two localities resulted from locality differences. In Izmir province, the karyotype formula was 7m+1sm whereas it was set as 4m+4sm in Muğla province. Our diploid counts are in agreement with the literature such as one of the reports from Turkish material and many others from different territories (
The section Reflexae is represented by a single species (Trigonella monspeliaca) in Turkey. This species was studied in two different localities. Diploid chromosome numbers are the same (2n=16) in both localities of Trigonella monspeliaca species. The chromosome sizes, total haploid chromosome lengths, arm lengths and centromeric indices are very close to each other while the karyotype formulae are different, i.e. the karyotype formula of Muğla province is 8m, of Antalya province is 5m+3sm. For
The section Isthmocarpae is represented by two species (Trigonella isthmocarpa and Trigonella rhytidocarpa) in Turkey. Karyology of the two species in this section was studied for the first time. The diploid chromosome number of Trigonella isthmocarpa and Trigonella rhytidocarpa species were found to be 2n=16=8m. Karyological characteristics of these two species are very close to each other.
The section Uncinatae is represented by two species (Trigonella spicata and Trigonella cephalotes) in Turkey. The diploid chromosome number of Trigonella spicata and Trigonella cephalotes were found to be 2n=16=5m+3sm. Karyological characteristics of these two species are very close to each other. There is a marked difference only in terms of total haploid chromosome length. While total haploid chromosome length was 10.36 μm in Trigonella spicata, that is 4.49 μm in Trigonella cephalotes. Trigonella cephalotes is also the species having the smallest haploid chromosome length among studied species.
The section Capitatae is represented by three species (Trigonella procumbens, Trigonella capitata and Trigonella pseudocapitata) in Turkey. The diploid chromosome number of Trigonella procumbens and Trigonella capitata was found as 2n=16=8m. Karyological characteristics of these two species are very close to each other. In addition to A chromosomes, two examples of B chromosomes were observed in Trigonella procumbens differing from the other species of the sections studied. The chromosome number of Trigonella procumbens was reported as 2n=18 by Yılmaz (2006) from the same locality, but he did not mention B chromosomes. The other species of the section, Trigonella pseudocapitata, could not be studied due to its inability to germination.
The section Biebersteinianae is represented by Trigonella coerulescens in Turkey. It was studied from two different localities. Diploid chromosome numbers are the same (2n=16=8m) in both localities of Trigonella coerulescens. Chromosome sizes, total haploid chromosome lengths, arm lengths and centromeric indices are very close to each other. The chromosome number of Trigonella coerulescens is in agreement with the previous report (2n=16) by Yılmaz (2006).
The section Foenum-graecum is represented by five species (Trigonella gladiata, Trigonella cariensis, Trigonella foenum-graecum, Trigonella macrorrhyncha and Trigonella cassia) in Turkey. Diploid chromosome numbers of the four species studied in this section are the same (2n=16). Trigonella cassia could not be studied due to failure to germinate. The chromosome morphologies of the species are very close to each other. The karyotype formula of Trigonella gladiata is 8m. In a cytological study performed by Bidak & Amin (1996), diploid chromosome number was found to be 2n=16 and 2n=18 in Trigonella gladiata. Researchers reported two different basic chromosome numbers for this species (x=8 and x=9). However, the basic chromosome number in our study was found to be x=8 for this species. In a cytological study conducted on Trigonella species,
We thank The Scientific and Technological Research Council of Turkey (TUBİTAK) (Project no. TBAG–2099 (101T142) and Scientific Investigation Project Coordinate of Selçuk University (project no: 05401046) for financial support.