a Male Hoplias malabaricus karyotype with conventional Giemsa staining (karyomorph A) b Metaphase corresponding to karyotype showed in (a) c Male meiotic metaphase showing formation of 21 bivalents d Female Hoplias malabaricus karyotype (karyomorph A) e C-banding karyotype exhibiting centromeric staining in most of chromosomes and telomeric signal in pairs number 14 and 20 f Ag-NOR banding karyotype displays telomeric signal in chromosome pair 20 gFISH with 18S rDNA probes labeling two chromosomic pairs, numbers 12 and 20 (boxed). Bar = 5 µm.