Pseudophryne coriacea macerated and cultured toe clippings A–C primary culture prior to cryopreservation (P0) D–F passage 1 cells, post cryopreservation (P1) A, B, D, E low power C, F high power A, B P0 cells form an expanding cluster (c) adjacent to the reference debris (d) at days 13 (D13) and D18 C P0 cells are either ovoid/polygonal (o) or spindle-shaped (s); rounded cells (r) are also observed D, E post-cryopreservation, P1 cells form two expanding mass reference points (m2 and m3) to reach approximately 70% confluency by D13 F post-cryopreservation P1 cells are both ovoid/polygonal (o) and spindle-shaped (s); rounded cells (r) are also observed. Scale bars: 10 μM.