Myrsine karyograms displaying 2n = 45 (a M. parvifolia and b M. coriacea) or 2n = 46 chromosomes (a–c the three species). In all M. parvifolia (a) and M. coriacea (b) individuals with 2n = 45, the odd chromosome number was well-marked by absence of the homologue pair of the chromosome 23. Metacentric and submetacentric chromosomes prevailing in the karyograms of the three species, with only one acrocentric chromosome was identified in M. coriacea (b chromosome 22). Although showing approximately 2C = 1.50 pg less DNA, M. parvifolia (a) displayed the same chromosome number in relation to the other species (b M. coriacea c M. umbellata). For all species, morphometric analyses showed identical, similar and distinct chromosome pairs with regard to morphometry and class. The similarity of some chromosomes was highlighted from the metacentric chromosome pairs 4 and 5 (d above) and submetacentric 15 and 16 (d below) of M. coriacea. In contrast, other chromosomes showed singular morphology, as the chromosome 1 and 2 of all species, the 22 of M. coriacea, which is the single acrocentric chromosome, and the chromosome 23. Bar = 5 µm.