Chromosomal locations of the 18S – 28S rRNA genes and (TTAGGG)n sequences in Monopterus albus. Hybridization pattern of FITC-labeled 18S – 28S rRNA genes (green) (a) and rhodamine-labeled TTAGGG repeats (red) (b) on DAPI-stained chromosomes, and their co-hybridization pattern (c). Hybridization pattern of FITC-labeled 18S – 28S rRNA genes (green) (d) on PI-stained chromosomes. PI-stained patterns of the same metaphase spreads of (d) is shown in (e). Arrowheads indicate FISH signals of the 18S – 28S rRNA genes. Arrows indicate the large PI-stained region. Scale =10 μm.