McGISH patterns and FISH analysis of chromosomes inSN19 (A), SN20 (C) and SN122 (E). Yellow denotes the A-genome chromosomes, gray indicates the B-genome chromosomes, red represents the D-genome chromosomes and green denotes the E. elongata chromosomes or chromosomal fragments. The asterisks indicate the wheat - E. elongata translocation chromosomes. FISH on the same metaphase chromosome spreads are simultaneously presented in lines SN19 (B), SN20 (D) and SN122 (F) by pTa535 (green) and pSc119.2 (red).