Mitotic chromosomes from mid gut of Boreonectes spp., arranged as karyotypes. a–e B. alpestris, a paratype ♂, Giemsa stained (from Dutton, Angus, (2007)) b, c ♂, Colle del Nivolet b Giemsa stained, c the same nucleus C-banded (from Angus (2010)) d, e San Bernardino, ♀, d Giemsa stained e the same nucleus C-banded f–k B. emmerichi f, g ♂ Yalashenshan f Giemsa stained g the same nucleus C-banded h, i ♀ Yalashenshan h Giemsa stained g the same nucleus C-banded j, k ♂ Mado j Giemsa stained k the same nucleus C-banded (from Angus et al (2015)) l–n B. macedonicus ♂♂ (from Angus (2008)) l Crno ezero Giemsa stained m Karanikoličko ezero Giemsa stained n the same nucleus C-banded o, p B. emmerichi ♂ shown in f, g q, r B. macedonicus Crete q ♂Giemsa stained r ♀C-banded (from Angus (2008). Scale = 5 μm.