FISH patterns of mitotic metaphase chromosomes detected using pAs1 probes (red) combined with 5S rDNA, 45S rDNA, and pSc119.2 probes in A. mongolicum (a–c); 5S rDNA, 45S rDNA, pSc119.2, AG, and AC in A. cristatum (2x) (d–h); 5S rDNA, 45S rDNA, pSc119.2, AAG, ACG, AGG, CAG, CAT, AG, and AC in A. cristatum (4x) (i–r). The pAs1 signals (red) were removed artificially in all images except in (a, d, i). Arrows indicate the target signals (green). Bar = 10μm.