FISH patterns of mitotic metaphase chromosomes detected using pAs1 probes (red) combined with 5S rDNA, 45S rDNA, pSc119.2, AG, and AC probes in A. desertorum (a–e); 5S rDNA, 45S rDNA, pSc119.2, AAG, ACT, AG, and AC in A. fragile (f–l); 5S rDNA, 45S rDNA, pSc119.2, CAT, AC, and AG in A. michnoi (m–r). The pAs1 signals (red) were removed artificially in all pictures except in (a, f, m). Arrows indicate the target signals (green). Scale bar = 10 μm.