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  • ContentsContents
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  • Article title
  • Abstract
  • Keywords
  • Introduction
  • Material and methods
    • Coral collection
    • Chromosome preparations
    • Chromosome length measurements
    • DNA extraction
    • PCR and DNA cloning
    • Probe preparation and FISH
    • Genomic DNA hybridization (GDH) using M. amakusensis sperm DNA
    • DNA sequencing and Homology search
    • Image acquisition and Processing
  • Results
    • Karyotype of M. amakusensis
    • GDH with whole-sperm DNA
    • Cloning, Sequence and FISH analysis of five markers
    • Comparison of sequences of MA-H3, MA-5S (5S U2RNA), and MA-18/28S with those of other species
  • Discussion
    • Cytogenetics
    • Genomic DNA Hybridization (GDH) using FISH
    • Physical mapping by FISH
    • Cloning and Sequence analysis
  • Authors’ contributions
  • Acknowledgments
  • References
  • ORCID
  • Supplementary materials
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