Idiogram (right) obtained from metaphase measurements of an F2 × Festulolium hybrid (2n = 34) after GISH analysis (left) with Lolium perenne genomic DNA labeled as a probe (Dig; green pseudocolor) and Festuca pratensis used as block DNA, counterstained with DAPI (blue pseudocolor). DRAWID measurements are shown for the recombinant chromosome 22 (arrow, CEN = centromere). Chromosome numbering is according to chromosome length starting with the largest chromosome.