Chromosomes of Rhampsinitus capensis (2n=34) (A-E) and Rhampsinitus discolor (F–K) (2n=24) after Giemsa staining (A, B, D, F, G, J) and after FISH with 18S rDNA (A and F partly, C, E, H, I, K). A male karyotype based on mitotic metaphase B, C female mitotic metaphase D, E female pachytene, bottom left inset shows a reconstruction of the heteromorphic bivalent; crosses indicate the subterminal position of 18S rDNA; arrowhead indicates bivalent with the expanded positive heteropycnotic area F male karyotype based on two sister cells of metaphase II G, H male mitotic metaphase I diakinesis J, K two sister cells of metaphase II. White arrows indicate the position of 18S rDNA and black arrows indicate the position after Giemsa staining. Asterisks indicate large signal of heteromorphic bivalent. Bar = 10 μm.