Immunochemistry with antibodies to ɤ-H2AX (red) and further FISH with telomeric PNA probe (CCCTAA)3 conjugated with FITC (green). Chromosomes stained DAPI (blue) A The metaphase spread of RNFF1 cell line on 9th passage B The metaphase spread of MR39 cell line on 6th passage. The arrows and asterisks indicate dysfunctional telomeres C – D. Curves of signal intensities along the length of telomeres containing dysfunctional telomeres on individual chromosomes from A–B respectively. Scale bars: 5 µm

 
 
  Part of: Zhdanova NS, Vaskova EA, Karamysheva TV, Minina JM, Rubtsov N, Zakian SM (2019) Dysfunction telomeres in embryonic fibroblasts and cultured in vitro pluripotent stem cells of Rattus norvegicus (Rodentia, Muridae). Comparative Cytogenetics 13(3): 197-210. https://doi.org/10.3897/CompCytogen.v13i3.34732