Fluorescence in situ hybridization in P. carvalhoi karyotype. a Hybridized with the 28S rDNA probe b the NOR-bearing chromosome highlighted by DAPI-staining, the Ag-NOR method, and FISH with 28S rDNA c In situ hybridization with the telomeric probe in the karyotype of P. carvalhoi from Pernambuco, Brazil. The arrows in c indicate the interstitial telomeric sequences (ITSs) and the constriction in chromosome 8 are indicates by asterisk.