GISH with Vigna umbellata genomic DNA probe (red) to the chromosomes of V. luteola (A, B), V. vexillata (C), V. minima (D, E), V. trilobata (F, G), and V. caracalla (H, I). A, D, F, H CPD banded chromosomes before the hybridization procedure. The chromosomes shown in C is the same spread shown in Figure 1C, D. The chromosomes showing interstitial CPD bands and pericentromeric DAPI+ bands in H are numbered according to the karyotype of this species. Arrowheads in B, C, E, G, I indicate the signals corresponding to the 45S rDNA sites. Arrows in H indicate the interstitial CPD bands. Scale bars: 10 μm.