Metaphase chromosomes of C. monoculus, P. scalare and S. discus hybridized with microsatellite sequences (a–m). Arrow shows no signs of hybridizations in a, b, h, i and chromosomes positive for microsatellite in l, m. The probes detection was performed with streptavidin Alexa Fluor 488 (green) or anti-digoxigenin rhodamine (red). Chromosomes were counterstained with DAPI. Scale bar: 10 µm.