Spreads of oocytes from intraspecific F1 hybrid E. tancrei (2n = 49, NF = 56). Immunostaining with antibodies to SCP 3 (green) and to the centromere (ACA, red). The yellow arrow marks the SC pentavalent a Early-mid pachytene oocyte: 21 SC bivalents, SC pentavalent, XX bivalent bSC pentavalent (yellow dotted line) from Fig. 2ac Scheme of SC pentavalent (see Figs 2a, b). The chromosomes forming the SC pentavalent shown in Fig. 1a. Scale bar: 5 μm.