Meiotic stages of Dichelops melacanthus (a–f), Loxa viridis (g–l) and Edessa collaris (m–r). (a, g and m) metaphase I by Giemsa conventional staining; (b, h and n) metaphase II by Giemsa conventional staining; (c, i and o) C-banding pachytenes; (d and j) metaphase I by DAPI staining; (p) diplotene by DAPI staining; (e and k) metaphase I by CMA3 staining; (q) diplotene by CMA3 staining; (f, l and r) Fluorescence in situ hybridization with digoxigenin-labeled 18S rDNA probe and counterstained with DAPI. X and Y correspond to the sex chromosomes. Arrows show heterochromatic marks in autosomes. Scale bar: 10 µm.