FISH signal by the probe from the microdissection-generated DNA probe of C. aspera’s hsr. A Metaphase cells with a distinct signal on the hsr (an arrowhead). Note that the hsr-derived probe hybridized not only with rRNA gene (arrowheads) but also with terminal ends of other chromosomes (arrows) in both full (2n=28, upper) and partial (lower) metaphases B The same cells were stained with DAPI. Chromosome microdissection of the hsr in chromosome 11 C Before microdissection of the hsrD After scraping of the hsr (an arrow). Scale bar: 5 μm.

 
 
  Part of: Taguchi T, Kubota S, Mezaki T, Tagami E, Sekida S, Nakachi S, Okuda K, Tominaga A (2016) Identification of homogeneously staining regions by G-banding and chromosome microdissection, and FISH marker selection using human Alu sequence primers in a scleractinian coral Coelastrea aspera Verrill, 1866 (Cnidaria). Comparative Cytogenetics 10(1): 61-75. https://doi.org/10.3897/CompCytogen.v10i1.5699