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  • ContentsContents
  • Article InfoArticle Info
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  • ErratumErratum
  • Article title
  • Abstract
  • Keywords
  • Introduction
  • Material and methods
    • Plant materials
    • Microdissection of X and Y chromosome in spinach
    • Construction of Y chromosome genomic subtraction library
    • Cloning of DNA subtraction library
    • Screening and identification of DNA subtractive library
    • Screening of repetitive DNA sequences
    • Chromosome localization of repeat DNA sequences
  • Results
    • Preparation of Tester DNA and Driver DNA
    • Cloning and screening DNA genomic subtractive library
    • Screening male-biased repetitive DNA sequences
    • Chromosome localization of repetitive DNA sequences
  • Discussion
    • The establishment of single chromosome subtractive hybridization technique in spinach
    • FISH localization of male-biased repetitive DNA sequences in spinach
  • Acknowledgements
  • References
  • Supplementary material
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