Metaphase plates of Gephyromantis striatus (A, A’, A”), Mantidactylus (Brygoomantis) alutus (B, B’, B”), Spinomantis prope aglavei “North” (C, C’, C”), Gephyromantis sp. Ca19 (D, D’, D”), Spinomantis sp. Ca3 (E, E’, E”) and Mantidactylus (Hylobatrachus) cowanii (F, F’, F”) stained with C-banding + Giemsa (A–F), + CMA (A’–F’) + DAPI (A”–F”). Arrows point at NORs while arrowheads highlight other heterochromatin blocks.