Somatic metaphase chromosomes and idiograms of Trichosanthes species stained with Giemsa (A, D, I, L), DAPI (C, E, J, M) and CMA3 (B, F, K, N) A–C T. cucumerina ssp. cucumerina (2n = 22), D–F Trichosanthes cucumerina ssp. cucumerina ‘Anguina’ (2n = 22) I–K T. dioica (male, 2n = 22) L–N T. dioica (female, 2n = 22). Arrows indicate satellited chromosomes in Giemsa plates and CMA+ve signals in B, F, K, N. Corresponding somatic idiograms (haploid set) of: G T. cucumerina ssp. cucumerina H Trichosanthes cucumerina ssp. cucumerina ‘Anguina’ O T. dioica male plant P T. dioica female plant. Blue and golden yellow bands in idiograms indicate DAPI+ve and CMA+ve signals, respectively. Scale Bars: 5 µm