Somatic metaphase chromosomes and idiograms of two Benincaseae species (2n = 24) stained with Giemsa (A, D, G), DAPI (B, E, H) and CMA3 (C, F, I) A–C Benincasa hispida D–F Coccinia grandis (female plant) G–I Coccinia grandis (male plant). Arrows indicate satellited chromosomes in Giemsa plates and distal CMA+ve signals in C, F, I. Note the longest Y chromosome without any CMA band in G–I and centromeric CMA+ve signals in F, I. Corresponding somatic idiograms (haploid set) of: J Benincasa hispida K Coccinia grandis (female plant) L Coccinia grandis (male plant) with CMA+ve (golden yellow) bands. Note the X chromosome remaining indistinguishable in L. Scale Bars: 5 µm