Synaptonemal complex of C. gomesi after immunodetection with anti-SCYP3 (green) and chromosome painting with B chromosome probe (red). a Male pachytene bearing two B chromosomes. Note the positive hybridization of the B paint probe on the bivalents of the B and Z chromosomes b Female diplotene bearing three B chromosomes. Note the presence of a B univalent and the ZW bivalent corresponding to the Z and W chromosomes, all positive for the B probe (right). Bar = 10 μm.