Pholcus, males, sex chromosome behavior in second meiotic division, Giemsa staining A P. silvai, two sister metaphases II. Metaphase II containing the X chromosomes is composed of 13 chromosomes. Metaphase II containing the Y chromosome comprises 12 chromosomes B P. alticeps, two sister anaphases II. Chromosomes X1 and Y display positive heteropycnosis. The X chromosomes are associated. The Y chromosome is placed in the middle of the half plates C P. fuerteventurensis, two sister metaphases II. Plate containing the X chromosomes is incomplete (1 chromosome missing). Note the positive heteropycnosis of the sex chromosomes. Abbreviations: X1 = X1 chromosome, X2 = X2 chromosome, Y = Y chromosome. Scale bars: 10 μm.