FISH molecular cytogenetic analysis with SSR probes. FISH on metaphase spreads of cardoon (left hand side) and of globe artichoke (right had side). The oligonucleotides sequence is indicated in each panel. Oligonucleotides were labelled with Cy3 (red fluorescence) or FITC (green fluorescence) fluorochromes. In (E) and (F) chromosomes 3, 5 and 8 of cardoon and globe artichoke, respectively, are indicated by arrows and can be discriminated by the widespread (GA)10 hybridization pattern on the long arms. Scale bars: 5 µm.