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Karyotypic description of the stingless bee Melipona quinquefasciata Lepeletier, 1836 (Hymenoptera, Meliponini) with emphasis on the presence of B chromosomes
expand article infoAlexandra Avelar Silva, Marla Piumbini Rocha§, Silvia das Graças Pompolo, Lucio Antonio de Oliveira Campos, Mara Garcia Tavares
‡ Universidade Federal de Viçosa, Viçosa, Brazil
§ Universidade Federal de Pelotas, Pelotas, Brazil
Open Access

Abstract

Stingless bees are distributed widely in the tropics, where they are major pollinators of several plant species. In this study, the karyotype of Melipona quinquefasciata Lepeletier, 1836 was analysed, with emphasis on the presence of B chromosomes. Post-defecating larvae were analysed using Giemsa staining, the C-banding technique, sequential staining with fluorochromes, and FISH. The chromosome number ranged from 2n = 18 to 22 (females) and from n = 9 to 13 (males) due to the presence of 0–4 B chromosomes. This result demonstrates that M. quinquefasciata has the same chromosomal number as other Melipona Illiger, 1806 species. Considering the A complement, heterochromatin was located only in the pericentromeric region of pair 1. Staining with chromomycin A3 (CMA3) and labelling with rDNA probe, indicated that this region corresponded to the nucleolus organising region. The B chromosomes of M. quinquefasciata could be found in individuals from different localities, they were completely heterochromatic (C-banding) and uniformly stained by 4’,6-diamidino-2-phenylindole (DAPI). Variations in the number of B chromosomes were detected between cells of the same individual, between individuals of the same colony, and between colonies from different localities.

Keywords

Cytogenetics, heterochromatin, karyotype, fluorochromes, FISH

Introduction

Classical or molecular cytogenetic analysis can be used to determine chromosome number and morphology, the location and quantity of AT or CG rich regions, nucleolus organizing regions, rRNA clusters and repetitive sequences in the genome. This information allows species characterization, identification of cryptic species and the mechanisms involved in their speciation, analysis of population variability, and studies on karyotype evolution, phylogeny and taxonomy of different groups of species (Rocha and Pompolo 1998, Lachowska et al. 2009, Mendes-Neto et al. 2010, Panzera et al. 2012, Mandrioli et al. 2014, Golub et al. 2016).

Such analysis can also identify intra-specific or numerical variations within a population due to the presence of B or extra chromosomes (Brito et al. 1997, Tosta et al. 2004, Martins et al. 2014). These chromosomes are usually heterochromatic, smaller than the normal complement chromosomes, and show a non-Mendelian segregation pattern. They have already been described in many animal and plant species, allowing for studies on their origin, stability and maintenance (Camacho 2005, Houben et al. 2014, Anjos et al. 2016).

In the order Hymenoptera, the presence of B chromosomes have already been reported in ants, wasps and bees. In ants, these chromosomes were detected in species of several genera (Lorite et al. 2002, Mariano et al. 2001, reviewed by Loiselle et al. 1990 and Gokhman 2009). In the parasitoid wasps, until now, these chromosomes were only found in Nasonia vitripennis Walker, 1836 (Pteromalidae), Trichogramma kaykai Pinto et Stouthamer, 1997 (Trichogrammatidae), Encarsia asterobemisiae Viggiani et Mazzone, 1980 (Aphelinidae) and in Pnigalio agraules Walker, 1830, P. gyamiensis Myartseva et Kurashev, 1990 and P. mediterraneus Ferrière & Delucchi, 1957 (Eulophidae) (Nur et al. 1988, Baldanza et al. 1999, Stouthamer et al. 2001, Gebiola et al. 2012, Gokhman et al. 2014). B chromosomes have also been identified in Trypoxylon albitarse Fabricius, 1804 (Crabronidae) (Araújo et al. 2000). Finally, in bees, B chromosomes have been reported in the genera Melipona Illiger, 1806 (M. rufiventris Lepeletier, 1836 and M. quinquefasciata Lepeletier, 1836), Partamona Schwarz, 1939 (P. cupira Smith, 1863, P. helleri Friese, 1900 and P. rustica Pedro et Camargo, 2003) and Tetragonisca Moure, 1946 (T. fiebrigi Schwarz, 1938) (revision in Tavares et al. 2017). They are also probably present in the species P. criptica Pedro et Camargo, 2003, P. seridoensis Pedro et Camargo, 2003, P. gregaria Pedro et Camargo, 2003, P. chapadicola Pedro et Camargo, 2003 and P. aff. helleri since molecular analysis demonstrated the presence of a sequence-characterized amplified region (SCAR) marker specific to the B chromosome of P. helleri in these genomes (Correia et al. 2014, Tosta et al. 2014, Machado et al. 2016). However, for these species, the presence of B chromosomes needs to be confirmed through cytogenetic techniques, as does the variation found in the sawfly Tenthedro brevicornis (Konow, 1886) (Sanderson 1970) and in the Braconidae, Aphidius ervi, Halliday, 1834 (Gokhman and Westendorff 2003).

The number of species with B chromosomes, however, increases as new species are studied cytogenetically (Camacho et al. 2000). For example, for many years it was considered that M. quinquefasciata had n = 18 and, consequently, 2n = 36 (Kerr 1972), a diploid number very different from that of most Melipona species surveyed so far (n = 9 and 2n = 18; revision in Tavares et al. 2017). However, Kerr (1972) probably examined a colony that was yielding diploid males (Tarelho 1973). Then, Pompolo (1992) reported that analysis of one colony of M. quinquefasciata showed 2n = 20 chromosomes. It was only when a cytogenetic analysis was carried out several years later that M. quinquefasciata was found to have the same chromosome number as the majority of other Melipona species, 2n = 18, and that the numeric variations found in the karyotype of this species (2n = 19–22 and n = 9–13) were attributed to the presence of different numbers of supernumerary chromosomes (Rocha 2002, Rocha et al. 2007). However, despite comparing the general characteristics of the karyotype of M. quinquefasciata with that of other Melipona species, Rocha et al. (2007) did not specifically described the karyotype of M. quinquefasciata, the banding patterns obtained, or the variation in the number of B chromosomes found.

Thus, in the present study, we combined the data obtained by Rocha (2002) for two colonies of M. quinquefasciata with the analysis of five other colonies in order to: 1) describe in detail the karyotype of M. quinquefasciata, including the chromosome number, morphology and the location of heterochromatic regions, regions rich in AT/CG and ribosomal genes, and (2) verify the existence of B chromosomes in colonies from different locations, as well as their variation within colonies.

Materials and methods

Biological material

Post-defecating M. quinquefasciata larvae obtained from a colony from Brasília, DF (15°46'47"S, 47°55'47"W) and one from Luziânia, GO (16°15'09"S, 47°57'01"W) were analysed in 2000–2002 (Rocha 2002). Later, in 2013, we analysed three more colonies from Bicas, MG (21°43'31"S, 43°03'34"W), and two from Januária, MG (15°29'17"S, 44°21'42"W; State Park of Veredas of Peruaçú, PEVP).

Chromosome preparation and treatments

Chromosome preparations (Imai et al. 1988) were obtained using cerebral ganglion cells of larvae in the final stage of defecation. The number of individuals and number of metaphases per individual analysed varied from colony to colony (Suppl. material 1: Table S1).

To determine the number and morphology of the chromosomes, conventional staining was performed using Giemsa diluted in Sorensen buffer at a ratio of 1:30, for 20 minutes. The C-banding technique was used for heterochromatin detection (Rocha and Pompolo 1998). Metaphases were analysed on an Olympus BX60 microscope and the karyotypes were assembled using Image-Pro Plus (Version 6.3, Media Cybernetics 2009). The chromosomes were classified according to Levan et al. (1964), and the karyotypes were arranged by pairing chromosomes in decreasing order of size.

Sequential staining with fluorochromes 4’,6-diamidino-2-phenylindole (DAPI) and chromomycin A3 (CMA3) was performed according to Schweizer (1980), using DAPI first for 30 min, followed by CMA3 for 1 h. The use of distamycin was omitted. The fluorescent in situ hybridisation (FISH) technique (Viegas-Pequignot 1992) was performed using the 45S rDNA probe pDm 238 (Roiha et al. 1981). The best images were captured by a CCD camera coupled to an Olympus BX-60 epifluorescence microscope, using excitation filters WB (λ = 330–385 nm) and WU (λ = 450–480 nm), under immersion and at 100× magnification.

Results and discussion

The chromosome number of M. quinquefasciata ranged from 2n = 18 to 22 in females and from n = 9 to 13 in males, as already described by Rocha et al. (2007). Its karyotypic formula was 2K = 10M + 6SM + 2A (Fig. 1). Thus, the typical chromosome number of M. quinquefasciata was the same found in most Melipona species (2n = 18; Tavares et al. 2017), and numeric variations are due to the presence of 0–4 B chromosomes in females and males (Fig. 2).

Figure 1.

Representative karyotype of Melipona quinquefasciata female, with three B chromosomes, stained with Giemsa. M, SM, A and B: metacentric, submetacentric, acrocentric and B chromosomes, respectively. Scale bar: 5μm.

Figure 2.

Melipona quinquefasciata metaphases, stained with Giemsa, showing the presence of 0, 1, 2, 3 and 4 B chromosomes (arrows). Scale bar: 5μm.

In the analysed colonies, the majority of individuals had B chromosomes (Suppl. material 1: Table S1). In samples from Brasília and Luziânia, for example, all females analysed showed at least one B chromosome and only four of the eight analysed males from Luziânia had cells without B chromosomes. Even in these four males, the number of cells with B chromosomes was much higher than the number of cells without them. Similarly, in the colonies from Bicas and Januária, the number of female cells without B chromosomes was very low.

Variations were also observed in the number of B chromosomes between cells of the same individual, between individuals of the same colony, and between colonies from different localities (Fig. 2; Suppl. material 1: Table S1). In samples from Januária, for example, all individuals with B chromosomes had two chromosomes of that kind, while in samples from Brasília, Luziânia and Bicas, individuals with 0, 1, 2, 3 or 4 B chromosomes were found. Intra- and intercolonial variations relating to the presence of B chromosomes have also been described in P. helleri, another stingless bee species. In this species, the number of B chromosomes can range from 0–7 between and within colonies and the size of the B chromosome can also vary among colonies from different geographic locations (Costa et al. 1992, Brito et al. 1997, 2005, Tosta et al. 2004, Martins et al. 2014). Likewise, in M. rufiventris a small B chromosome was found in a few individuals (males and females) from one of the six colonies analysed (Lopes et al. 2008). Marthe et al. (2010) also described the presence of one B chromosome in some individuals of two colonies of P. cupira and Barth et al. (2011) observed that colonies of Tetragonisca fiebrigi can harbour individuals with 0, 1 or 2 B chromosomes. Together, our data and these published reports demonstrated that intra- and intercolony variation in the number of B chromosomes is common in stingless bees.

In different individuals and in the analysed colonies as a whole, the number of cells carrying two (411 cells) or three (268 cells) B chromosomes was considerably higher than those that had four B chromosomes (34 cells; Suppl. material 1: Table S1), as previously observed for P. helleri (Costa et al. 1992, Brito et al. 1997, Tosta et al. 2004). A more extensive cytogenetic analysis further demonstrated the presence of up to 7 B chromosomes in some P. helleri individuals (Martins et al. 2014) and, it is possible that analysis of colonies from other localities may change our perspective on B chromosome numbers for M. quinquefasciata. Such analysis could provide insight as to whether there is a mechanism restricting the number of B chromosomes in stingless bees, as originally proposed by Martins et al. (2013). Interestingly, no study has reported a positive or negative effect on fitness related to the presence of different numbers of B chromosomes in this or other Meliponini species, as has been found for some other taxa (Camacho 2005).

Our data also revealed that, in M. quinquefasciata, the heterochromatin, identified by the C-banding technique, was located only in the pericentromeric region of pair 1 (Fig. 3a). Similar results have already been described for other Melipona species, such as M. marginata Lepeletier, 1836 (Maffei et al. 2001), M. asilvai Moure, 1971 (Rocha et al. 2002), M. compressipes (Fabricius, 1804) (Rocha et al. 2002), M. rufiventris, and M. mondury Smith, 1863 (Lopes et al. 2008). Therefore, it was possible to infer that the chromosomes of the A complement of M. quinquefasciata had low heterochromatin content. As the genus Melipona can be separated in two groups, one with low (Group I) and the other with high (Group II) heterochromatin amounts, M. quinquefasciata could be grouped into Group I together with M. marginata, M. quadrifasciata Lepeletier, 1836, M. bicolor Lepeletier, 1836, M. asilvai, M. subnitida Ducke, 1910, M. mandacaia Smith, 1863 and M. puncticolis Friese, 1902 (Rocha and Pompolo 1998, Rocha et al. 2002).

Figure 3.

Melipona quinquefasciata metaphase with 2n = 18 + 2Bs submitted to C-banding (a), CMA3 (b) and DAPI (c) staining, and to the FISH technique (d). The arrows indicate the rDNA location, while asterisks indicate the B chromosomes and arrowheads indicate an interphase nucleus with two signals. Scale bar: 5 μm.

However, M. quinquefasciata belongs to the subgenus Melikerria Moure, 1992 and species clustered in Group I belong to the subgenera Melipona Illiger, 1806 or Eomelipona Moure, 1992; Group II clusters species of the subgenera Melikerria and Michmelia Moure, 1975 (Lopes et al. 2011). Additionally, M. fasciculata Smith, 1854 and M. interrupta Latreille, 1811, the only other species of the subgenus Melikerria that had their heterochromatin distribution pattern analysed, presented high heterochromatin quantities and were included in Group II (Lopes et al. 2011). This reinforces the need of additional cytogenetic studies concerning species of this subgenus.

By comparison, the B chromosomes of M. quinquefasciata were completely heterochromatic, as shown by the C-banding technique (Fig. 3a) and Giemsa staining (Fig. 1), regardless their number in the examined metaphases (Fig. 2). The staining with DAPI confirmed the heterochromatic nature of these chromosomes (Fig. 3c), indicating that, unlike the chromosomes of the A complement, B chromosomes of M. quinquefasciata were rich in AT base pairs. Unfortunately, due to their heterochromatic nature, it was not possible to study the morphology of B chromosomes of M. quinquefasciata in detail.

CMA3 staining and FISH analysis using a 45S rDNA probe confirmed that ribosomal genes were located only in the pericentromeric region of pair 1 in the karyotype of M. quinquefasciata (Fig. 3b, d), as already reported for the two colonies analysed by Rocha et al. (2007). The presence of a unique autosome pair with a nucleolus organizer in M. quinquefasciata corroborated previous reports about the location of the rDNA clusters in other Melipona species, independent of the technique used (Ag-NOR impregnation, CMA3 staining or FISH; Rocha et al. 2002, Brito et al. 2003, Lopes et al. 2011, Cunha et al. 2018, Piccoli et al. 2018). This seemed to be the most frequent pattern found in other Meliponini genera (Brito-Ribon et al. 1999, Rocha et al. 2003, Krinski et al. 2010), although the presence of multiple rDNA clusters has also been described (Rocha et al. 2003, Brito et al. 2005, Duarte et al. 2009, Martins et al. 2009, Godoy et al. 2013).

Conclusion

The results of this study demonstrated that M. quinquefasciata has an A complement with a chromosome number characteristic of the Melipona genus (2n = 18; n = 9) and a karyotypic formula of 2K = 10M + 6SM + 2A. The numerical variation frequently described for this species might be explained by the presence of a variable number of B chromosomes in individual karyotypes. These chromosomes were found in individuals from different localities and were completely heterochromatic. By comparison, in the chromosomes of the A complement heterochromatin was located only in the pericentromeric region of pair 1, which corresponded to the nucleolus organising region, as demonstrated by CMA3 staining and in situ hybridisation using a 45S rDNA probe.

Acknowledgements

We are grateful to Paula São Thiago for providing us with some of the M. quinquefasciata samples used in this study. We also wish to thank the Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) for financial support.

References

  • Anjos A, Loreto V, Cabral de Mello DC (2016) Organization of some repetitive DNAs and B chromosomes in the grasshopper Eumastusia koebelei (Rehn, 1909) (Orthoptera, Acrididae, Leptysminae). Comparative Cytogenetics 10: 219–228. http://doi.org/10.3897/CompCytogen.v10i2.7609
  • Araújo SM, Pompolo SG, Dergam JAS, Campos LAO (2000) The B chromosome system of Trypoxylon (Trypargilum) albitarse (Hymenoptera, Sphecidae) 1. Banding analysis. Cytobios 101: 7–13.
  • Baldanza F, Gaudio L, Viggiani G (1999) Cytotaxonomic studies of Encarsia Foerster (Hymenoptera: Aphelinidae). Bulletin of Entomological Research 89: 209–215. https://doi.org/10.1017/S0007485399000322 S1415
  • Barth A, Fernandes A, Pompolo SG, Costa MA (2011) Occurrence of B chromosomes in Tetragonisca Latreille, 1811 (Hymenoptera, Apidae, Meliponini): a new contribution to the cytotaxonomy of the genus. Genetics and Molecular Biology 34: 76–79. http://dx.doi.org/10.1590/S1415-47572010005000100
  • Brito RM, Costa MA, Pompolo SG (1997) Characterization and distribution of supernumerary chromosomes in 23 colonies of Partamona helleri (Hymenoptera, Apidae, Meliponinae). Brazilian Journal of Genetics 20: 185–188.
  • Brito RM, Caixeiro APA, Pompolo SG, Azevedo GG (2003) Cytogenetic data of Partamona peckolti (Hymenoptera, Apidae, Meliponini) by C-banding and fluorochrome staining with DA/CMA3 and DA/DAPI. Genetics and Molecular Biology 26: 53–57. http://dx.doi.org/10.1590/S1415-47572003000100009
  • Brito RM, Pompolo SG, Magalhães MFM, Barros EG, Sakamoto-Hojo ET (2005) Cytogenetic characterization of two Partamona species (Hymenoptera, Apidae, Meliponini) by fluorochrome staining and localization of 18S rDNA clusters by FISH. Cytologia 70: 373–380. http://doi.org/10.1508/cytologia.70.373
  • Brito-Ribon RM, Miyazawa CS, Pompolo SG (1999) First karyotype characterization of four species of Partamona (Friese, 1980) (Hymenoptera, Apidae, Meliponini) in Mato Grosso State, Brazil. Cytobios 100: 19–26.
  • Costa MA, Pompolo SG, Campos LAO (1992) Supernumerary chromosomes in Partamona cupira (Hymenoptera, Apidae, Meliponinae). Revista Brasileira de Genética 15: 801–806.
  • Cunha MS, Travenzoli NM, Ferreira RP, Cassinela EK, Silva HB, Oliveira FPM, Fernandes-Salomão TM, Lopes DM (in press) Comparative cytogenetics in three Melipona species (Hymenoptera: Apidae) with two divergent heterochromatic patterns. Genetics and Molecular Biology.
  • Duarte OMP, Martins CCC, Waldschmidt AM, Costa MA (2009) Occurrence of multiple nucleolus organizer regions and intraspecific karyotype variation in Scaptotrigona xantrotricha Moure (Hymenoptera, Meliponini). Genetics and Molecular Research 8: 831–839. http://doi.org/10.4238/vol8-3gmr598
  • Gebiola M, Giorgini M, Navone P, Bernardo U (2012) A karyological study of the genus Pnigalio Schrank (Hymenoptera: Eulophidae): Assessing the taxonomic utility of chromosomes at the species level. Bulletin of Entomological Research 102: 43–50. http://doi.org/10.1017/S0007485311000356
  • Godoy DC, Ferreira RP, Lopes DM (2013) Chromosomal variation and cytogenetics of Plebeia lucii and P. phrynostoma (Hymenoptera: Apidae). Florida Entomologist 96: 1559–1566. https://doi.org/10.1653/024.096.0439
  • Gokhman VE, Westendorff M (2003) Chromosomes of Aphidius ervi Haliday, 1834 (Hymenoptera: Braconidae). Beitrӓge zur Entomologie 53: 161–165.
  • Gokhman VE, Yefremova ZA, Yegorenkova EN (2014) Karyotypes of parasitic wasps of the family Eulophidae (Hymenoptera) attacking leaf-mining Lepidoptera (Gracillariidae, Gelechiidae). Comparative Cytogenetics 8: 31–41. http://doi.org/10.3897/CompCytogen.v8i1.6537
  • Golub NV, Golub VB, Kuznetsova VG (2016) Further evidence for the variability of the 18S rDNA loci in the family Tingidae (Hemiptera, Heteroptera). Comparative Cytogenetics 10: 517–527. http://doi.org/10.3897/CompCytogen.v10i4.9631
  • Houben A, Banaei-Moghaddam AM, Klemme S, Timmis JN (2014) Evolution and biology of supernumerary B chromosomes. Cellular and Molecular Life Sciences: 71: 467–478. https://doi.org/10.1007/s00018-013-1437-7
  • Imai HT, Taylor RW, Crosland MWJ, Crozier RH (1988) Modes of spontaneous evolution in ants with reference to the minimum interaction hypothesis. Japanese Journal of Genetics 63: 159–185. https://doi.org/10.1266/jjg.63.159
  • Krinski D, Fernandes A, Rocha MP, Pompolo SG (2010) Karyotypic description of the stingless bee Oxytrigona cf. flaveola (Hymenoptera, Apidae, Meliponina) of a colony from Tangará da Serra, Mato Grosso State, Brazil. Genetics and Molecular Biology 33: 494–498. http://dx.doi.org/10.1590/S1415-47572010000300020
  • Lachowska D, Rozek M, Holecová M (2009) Chromosomal similarities and differences among three sibling species of the Acalles echinatus group (Coleoptera, Curculionidae, Crypthorhynchinae). Zootaxa 1985: 63–68. www.mapress.com/zootaxa
  • Loiselle R, Francoeur VA, Fischer K, Buschinger A (1990) Variations and taxonomic significance of the chromosome numbers in the Nearctic species of the genus Leptothorax (s.s.) (Formicidae: Hymenoptera). Caryologia 43: 321–334. https://doi.org/10.1080/00087114.1990.10797010
  • Lopes DM, Pompolo SG, Tavares MG, Campos LAO (2008) Cytogenetic characterization of Melipona rufiventris Lepeltier 1836 and Melipona mondury Smith 1863 (Hymenoptera, Apidae) by C banding and fluorochromes. Genetics and Molecular Biology 31: 49–52. http://dx.doi.org/10.1590/S1415-47572008000100010
  • Lopes DM, Fernandes A, Praça-Fontes MM, Werneck HA, Resende HC, Campos LAO (2011) Cytogenetics of three Melipona species (Hymenoptera, Apidae, Meliponini). Sociobiology 58: 185–194.
  • Lorite P, Carrilo JA, Tinaut A, Palomeque T (2002) Chromosome numbers in Spanish Formicidae IV. New data of species from the genera Camponotus, Formica, Lasius, Messor, and Monomorium. Sociobiology 40: 331–341.
  • Machado DP, Miranda EA, Dessi MC, Sabadini CP, Del Lama MA (2016) Occurrence and origin of supernumerary chromosomes in Partamona (Hymenoptera: Apidae: Meliponini). Cytogenetic and Genome Research 150: 68–75. http://doi.org/10.1159/000452290
  • Maffei EM, Pompolo SG, Silva-Junior JC, Caixeiro AP, Rocha MP, Dergam JA (2001) Silver staining of nucleolar organizer regions (NOR) in some species of Hymenoptera (bees and parasitic wasp) and Coleoptera (lady-beetle). Cytobios 104: 119–125.
  • Mandrioli M, Zansi F, Manicardi GC (2014) Karyotype rearrangements and telomere analysis in Myzus persicae (Hemiptera, Aphididae) strains collected on Lavandula sp. plants. Comparative Cytogenetics 8: 259–274. http://doi.org/10.3897/CompCytogen.v8i4.8568
  • Mariano CSF, Pompolo SG, Delabie JHC, Campos LAO (2001) Estudos cariotípicos de algumas espécies neotropicais de Camponotus Mayr (Hymenoptera, Formicidae). Revista Brasileira de Entomologia 45: 267–274.
  • Marthe JB, Pompolo SG, Campos LAO, Salomão TMF, Tavares MG (2010) Cytogenetic characterization of Partamona cupira (Hymenoptera, Apidae) by fluorochromes. Genetics and Molecular Biology 33: 253–255. http://dx.doi.org/10.1590/S1415-47572010005000029
  • Martins CCC, Diniz D, Sobrinho-Scudeler PE, Foresti F, Campos LAO, Costa MA (2013) Investigation of Partamona helleri (Apidae, Meliponini) B chromosome origin. An approach by microdissection and whole chromosome painting. Apidologie 44: 75–81. http://doi.org/10.1007/s13592-012-0157-6
  • Martins CCC, Duarte OMP, Waldschmidt AM, Alves RMO, Costa MA (2009) New occurrence of B chromosomes in Partamona helleri (Friese, 1900) (Hymenoptera, Meliponini). Genetics and Molecular Biology 32: 782–785. http://dx.doi.org/10.1590/S1415-47572009005000065
  • Martins CCC, Waldschmidt AM, Costa MA (2014) Unprecedented record of ten novel B chromosomes in the stingless bee Partamona helleri (Apidae, Meliponini). Apidologie 45: 431–439. http://doi.org/10.1007/s13592-013-0257-y
  • Mendes-Neto EO, Vicari MR, Campaner C, Nogaroto V, Artoni RF, Almeida MC (2010) Cytogenetic analysis of Astylus antis (Perty, 1830) (Coleoptera, Melyridae): karyotype, heterochromatin and location of ribosomal genes. Genetics and Molecular Biology, 33: 237–243. http://dx.doi.org/10.1590/S1415-47572010005000050
  • Nur U, Werren JH, Eickbush DG, Burke WD, Eickbush TH (1988) A selfish B chromosome that enhances its transmission by eliminating the paternal genome. Science 240: 512–514. http://doi.org/10.1126/science.3358129
  • Panzera Y, Pita S, Ferreiro MJ, Ferrandis I, Lages C, Pérez R, Silva AE, Guerra M, Panzera F (2012) High dynamics of rDNA cluster location in kissing bug holocentric chromosomes (Triatominae, Heteroptera). Cytogenetic and Genome Research 138: 56–67. http://doi.org/10.1159/000341888
  • Piccoli MCA, Bardella VB, Cabral-de-Mello DC (2018) Repetitive DNAs in Melipona scutellaris (Hymenoptera: Apidae: Meliponidae): Chromosomal distribution and test of multiple heterochromatin amplification in the genus. Apidologie http://doi.org/10.1007/s13592-018-0577-z
  • Pompolo SG (1992) Estudos citogenéticos em Meliponinae. Naturalia (special issue): 62–66.
  • Rocha MP (2002) Análises citogenéticas em abelhas do gênero Melipona (Hymenoptera, Meliponini). Thesis. Universidade Federal de Viçosa, 84pp. [In Portuguese].
  • Rocha MP, Pompolo SG, Campos LAO (2003) Citogenética da tribo Meliponini (Hymenoptera, Apidae). Homenagem aos 90 anos de Jesus Santiago Moure. UNESC, Criciúma, 311–320.
  • Rocha MP, Pompolo SG, Fernandes A, Campos LAO (2007) Melipona–seis décadas de citogenética. Bioscience Journal 23: 11–117.
  • Roiha H, Miller JR, Woods LC, Glover D (1981) Arrangements and rearrangements of sequences flanking the two types of rDNA insertion in D. melanogaster. Nature 290: 749–753. http://doi.org/10.1038/290749a0
  • Sanderson AR (1970) Further studies on the cytology of sawflies. Proceedings of the Royal Society of Edinburgh B 71: 29–40.
  • Schweizer D (1980) Simultaneous fluorescent staining of R bands and specific heterocromatic regions (DA/DAPI-bands) in human chromosomes. Cytogenetics and Cell Genetics 27: 190–193. http://doi.org/10.1159/000131482
  • Stouthamer R, Tilborg M, Jong JH, Nunney L, Luck RF (2001) Selfish element maintains sex in natural populations of a parasitoid wasp. Proceedings of the Royal Society of London B 268: 617–622. https://doi.org/10.1098/rspb.2000.1404
  • Tarelho ZVS (1973) Contribuição ao estudo citogenético dos Apoidea. Dissertation, Universidade de São Paulo, Ribeirão Preto, 113 pp. [In Portuguese]
  • Tosta VC, Fernandes-Salomão TM, Tavares MG, Pompolo SG, Barros EG, Campos LAO (2004) A RAPD marker associated with B chromosomes in Partamona helleri (Hymenoptera, Apidae). Cytogenetics and Genome Research 106: 279–283. http://doi.org/10.1159/000079299
  • Tosta VC, Marthe JB, Tavares MG, Fernandes-Salomão TM, Pompolo SG, Recco-Pimentel SM, Perfectti F, Campos LAO, Camacho JPM (2014) Possible introgression of B chromosomes between bee species (genus Partamona). Cytogenetic and Genome Research 144: 220–226. https://doi.org/10.1159/000370171
  • Viegas-Péquignot E (1992) In situ hybridization to chromosomes with biotinylated probes. In: Willernson D (Ed.) In situ hybridization: a practical approach. Oxford University Press, Oxford, 137–158.