Short Communication |
Corresponding author: Ilya A. Gavrilov-Zimin ( coccids@gmail.com ) Academic editor: Valentina G. Kuznetsova
© 2018 Ilya A. Gavrilov-Zimin.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Gavrilov-Zimin IA (2018) First illustration of chromosomes and genetic system of Lecanodiaspidinae (Homoptera, Coccinea, Asterolecaniidae s.l.). Comparative Cytogenetics 12(3): 439-443. https://doi.org/10.3897/CompCytogen.v12i3.29648
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The karyotype of Psoraleococcus multipori (Morrison, 1921) was studied for the first time, based on material from Indonesia (Sulawesi). The diploid chromosome number was found to be 18 in both males and females, but some cells contained also additional small chromosomal elements, probably B chromosomes. About 50 % of the studied embryos demonstrated paternal genome heterochromatinization of one haploid set of chromosomes (PGH) suggesting presence of a Lecanoid genetic system. The embryos with PGH are known to be always the male embryos in scale insects and so, bisexual reproduction may be presumed for P. multipori. The information provided represents the first probative cytogenetic data for the subfamily Lecanodiaspidinae Targioni Tozzetti, 1896 as a whole. A detailed morphological figure and photos of female and male embryonic cells are given. Additionally, it was discovered that the females of P. multipori exhibit complete ovoviviparity.
Psoraleococcus multipori , scale insects, morphology, karyotype, Lecanoid system
The subfamily Lecanodiaspidinae Targioni Tozzetti, 1896 comprises 12 genera and about 80 species in the world fauna (
Lecanodiaspidinae was almost unstudied previously in respect of cytogenetics. The chromosomal number (2n=14) was reported for one species only, Anomalococcus indicus Ramakrishna Ayyar, 1919 by
During an expedition in Sulawesi Is. (Indonesia) the author was able to collect the series of adult females of Psoraleococcus multipori (Morrison, 1921): K 923, vicinity of Kendari, on branch of undetermined dicotyledonous tree, inside of ant gallery, 10.XI.2011, I. Gavrilov-Zimin (deposited at Zoological Institute RAS, St. Petersburg). These females appeared to be suitable for preparing both morphological and chromosomal slides. The method of preparation of the morphological slides and method of squashing of the embryonic cells in lactoacetorcein for chromosomal studies see, for example, in
The diploid chromosomal number of P. multipori is 18 in both sexes (Figs
The females of P. multipori exhibit complete ovoviviparity, i.e. all stages of embryonic development occur inside the maternal body (see a review of reproductive strategies of scale insects and appropriate terminology in
The work was performed in the frame of the state research project no. AAAA-A17-117030310018-5 at Zoological Institute, Russian Academy of Sciences.
Embryonic cells of Psoraleococcus multipori. a female embryo, 2n = 18 b female embryo, 2n = 18 + 2B (B-chromosomes arrowed) c male embryo, paternal set of chromosomes begins heterochromatinization (arrowed) d Lecanoid heterochromatinization (arrowed) in interphase cells of male embryo; e metaphase and anaphase in female embryo showing no lagging chromosomes (Bs) f karyogram, prepared basing on the Fig.