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Research Article
Chromosome complements of Channa lucius and C. striata from Phu Quoc Island and karyotypic evolution in snakehead fishes (Actinopterygii, Channidae)
expand article infoDenis V. Prazdnikov‡
‡ Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow, Russia

Corresponding author: Denis V. Prazdnikov ( pdvfish3409@rambler.ru )

Academic editor: Ilya Gavrilov-Zimin
© 2023 Denis V. Prazdnikov.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation: Prazdnikov DV (2023) Chromosome complements of Channa lucius and C. striata from Phu Quoc Island and karyotypic evolution in snakehead fishes (Actinopterygii, Channidae). Comparative Cytogenetics 17(1): 1-12. https://doi.org/10.3897/compcytogen.v17.i1.94943
ZooBank: urn:lsid:zoobank.org:pub:4A6D0293-36DA-4C38-951B-94970D332FFB
Open Access

Abstract

Snakehead fishes of the family Channidae are obligatory air-breathers freshwater predators, the vast majority of which belong to the genus Channa Scopoli, 1777. Channa species are characterized by high karyotypic diversity due to various types of chromosomal rearrangements. It is assumed that, in addition to the lifestyle, fragmentation and isolation of snakehead populations contribute to an increase in karyotypic diversity. However, the chromosome complements of many isolated populations of widespread Channa species remain unknown, and the direction of karyotype transformations is poorly understood. This paper describes the previously unstudied karyotypes of Channa lucius (Cuvier, 1831) and C. striata (Bloch, 1793) from Phu Quoc Island and analyzes the trends of karyotypic evolution in the genus Channa. In C. lucius, the karyotypes are differed in the number of chromosome arms (2n = 48, NF = 50 and 51), while in C. striata, the karyotypes are differed in the diploid chromosome number (2n = 44 and 43, NF = 48). A comparative cytogenetic analysis showed that the main trend of karyotypic evolution of Channa species is associated with a decrease in the number of chromosomes and an increase in the number of chromosome arms, mainly due to fusions and pericentric inversions. The data obtained support the assumption that fragmentation and isolation of populations, especially of continental islands, contribute to the karyotypic diversification of snakeheads and are of interest for further cytogenetic studies of Channidae.

Keywords

Channa species, chromosomal rearrangements, karyotype differentiation, snakeheads

Introduction

The family Channidae includes two genera of freshwater snakehead fishes (Parachanna Teugels et Daget, 1984 and Channa Scopoli, 1777) with a disjunct range (Courtenay and Williams 2004; Rüber et al. 2020). The genus Parachanna is restricted to tropical Africa and contains three species. The genus Channa is more numerous in terms of the number of species (more than 40 species described to date) distributed mainly in Southern Asia (Fricke et al. 2022; Froese and Pauly 2022). The estimated number of species in this genus varies as the group is subject to frequent taxonomic revisions and the currently accepted nominal species may constitute species complexes (Adamson et al. 2010; Cioffi et al. 2015; Conte-Grand et al. 2017).

Appearing in the Eocene, snakehead fishes have undergone a long evolution with multiple range expansions and repeated contacts with lineages that had diverged in isolation (Adamson et al. 2010; Rüber et al. 2020), reflected in their karyotypic diversity. Among the cytogenetically studied Channa species, the number of chromosomes varies from 2n = 32 to 2n = 112, while the number of chromosome arms from NF = 46 to NF = 116 (Kumar et al. 2019). Given such high karyotypic diversity, it is obvious that the evolutionary dynamism in the genus Channa is a result of various types of chromosomal rearrangements, the main of which are pericentric inversions, fusions, and polyploidization (Dhar and Chatterjee 1984; Rishi and Haobam 1990; Tanomtong et al. 2014; Cioffi et al. 2015). At the same time, the trends of karyotypic evolution remain poorly understood.

In the course of evolution, snakehead fishes developed accessory air-breathing organs, which allows them to do without water for a long time and migrate over land to colonize new habitats (Sayer 2005; Bressman et al. 2019). These features have contributed to the distribution of snakeheads in a suitable climate zone, in particular species, such as Channa lucius and C. striata, which have relatively large ranges in South Asia, including many freshwater habitats on both mainland and islands (Adamson et al. 2010; Tan et al. 2012). The wide geographical distribution associated with the lifestyle of Channa species, together with complex hydrographic and geological events in their habitats, led to the fragmentation and isolation of populations (Adamson et al. 2012; Tan et al. 2012; Robert et al. 2019), which in turn could have contributed to karyotypic changes. For example, the geographical separation of South Asian populations of C. punctata contributed to the fixation of various types of chromosomal rearrangements in different parts of the range, which led to karyotypic variability from 2n = 32 (NF = 58–64) to 2n = 34 (NF = 64) (Ruma et al. 2006; Kumar et al. 2013; Rakshit et al. 2015). Interpopulation chromosomal variability found among the cytogenetically studied snakehead species has led to the assumption that lifestyle, fragmentation, and isolation of populations contribute to an increase in karyotypic diversity (Cioffi et al. 2015). In this regard, it is of interest to study karyotypes in previously unexplored small and/or isolated populations of widespread Channa species.

This study presents chromosome complements of C. lucius and C. striata from Phu Quoc Island and comparative cytogenetic analysis (chromosome number and karyotype composition) of the genus Channa. The trends in the karyotypic evolution of snakeheads and chromosomal diversification due to the isolation of island populations are discussed.

Material and methods

Individuals of Channa species were collected from Phu Quoc Island (Gulf of Thailand, Vietnam) (Fig. 1) in December of 2011. Four individuals (two females and two males) of C. lucius (Cuvier, 1831) were karyotyped from the Bai Dai River and Duong Dong River basins (Fig. 1). Six individuals (two females and four males) of C. striata (Bloch, 1793) were karyotyped from the Bai Dai River basin (Fig. 1). Snakehead vouchers were deposited in the Southern Department of the Vietnam-Russian Tropical Center (Ho Chi Minh City). The total number of metaphase plates studied for each species was 65 and 82, respectively.

Figure 1.

Map showing the location of Phu Quoc Island (left) and island details with Channa species collection sites (right).

Chromosome preparations were obtained from the anterior part of the kidney according to previously published methods (Ojima and Kurishita 1980; Blanco et al. 2012) with the initial treatment of live fish with colchicine (injection of 0.08% solution into the spinal muscle). The anterior kidney tissue was incubated in 0.075 M KCl (hypotonic solution) for 24 min at 28 °C and fixed in 96% ethanol mixed with glacial acetic acid (3:1 ratio). Chromosome preparations made using standard air-drying techniques were stained with 5% Giemsa solution in phosphate buffer at pH 6.8 for 7 min. Mitotic chromosomes were analyzed under a microscope Leica DM 1000 with DFC 295 camera and LAS EZ software. Chromosomes were classified as metacentric (m), submetacentric (sm), subtelocentric (st), and acrocentric (a) according to their arm ratios (Levan et al. 1964). To determine the number of chromosome arms (NF), chromosomes of the m and sm groups were considered biarmed and those of the st/a group uniarmed. For statistical analysis of the results and data visualization, I used Excel 2021 software. The regression between the proportion of biarmed chromosomes and diploid chromosome number, and the Spearman correlation were calculated.

Results and discussion

Karyotypic diversity in Channa lucius and C. striata

For C. lucius from both studied localities of Phu Quoc Island, the same diploid number of 2n = 48 was characteristic, but a different karyotype composition. In individuals from the Bai Dai River basin, the karyotype consisted of 2 metacentric chromosomes (m) and 46 subtelocentric and acrocentric chromosomes (st/a), NF = 50 (Fig. 2A). The karyotype of individuals from the Duong Dong River basin consisted of 3m and 45 st/a, NF = 51 (Fig. 2B). C. striata from the Bai Dai River basin had karyotypes differing in the number of biarmed chromosomes with 2n = 44 (2m+2sm+40st/a) and 2n = 43 (3m+2sm+38st/a), NF = 48 (Fig. 2C, D). In the two studied species, no differences were observed between male and female karyotypes.

Figure 2.

Karyotypes of Channa lucius (A, B) and C. striata (C, D) from Phu Quoc Island. Numerals indicate the paired chromosomes. Scale bar: 5 μm.

Comparative analysis of island and mainland populations of C. lucius showed interpopulation chromosomal variability. Populations from Thailand and Phu Quoc Island differed in the number of m-chromosomes (Table 1), which is probably due to pericentric inversion. Populations of C. striata were characterized by different levels of chromosomal polymorphism. Previous studies of mainland populations of C. striata have shown marked karyotypic variability ranging from 2n = 40 to 2n = 44 (Table 1). For a population from Northeastern Thailand, an atypical karyotype with 2n = 43 was found containing an unpaired large m-chromosome (Cioffi et al. 2015). It is assumed that individuals with 2n = 43 could have arisen both as a result of hybridization of two parental karyotypes with 2n = 44 and 2n = 42, and as a result of centric fusion of chromosomes in C. striata with 2n = 44 (Cioffi et al. 2015). In the polymorphic population of C. striata from Phu Quoc Island, the karyomorph with 2n = 43 was heterozygous for centric fusion. The maintenance and preservation of such a heterozygous state with an unpaired m-chromosome in different populations of C. striata may be evidence in favor of the fact that individuals with 2n = 43 produce viable gametes. Interestingly, on Phu Quoc Island, heterozygous karyotypes were also found in goby fish (Prazdnikov 2018). Previous studies have revealed the important role of heterozygous chromosomal rearrangements in maintaining karyotypic diversity in different groups of animals (Guerrero and Kirkpatrick 2014; Dobigny et al. 2017; Llaurens et al. 2017; Wellenreuther and Bernatchez 2018).

Table 1.
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CSV
XLSX

Diploid chromosome number (2n), chromosome arm number (NF), karyotype structure, and collection site of Channa lucius and C. striata.

Species 2n NF Karyotype structure Locality References
C. lucius 48 50 2m+46st/a Northeastern Thailand (Bung Klua reservoir in the Roi-Et) Khakhong et al. 2014
48 52 2m+2sm+2st+42a Thailand Donsakul and Magtoon 1991
48 52 4m/sm/st+44a Southern Thailand (Tapi Basin) Cioffi et al. 2015
48 50 2m+46st/a Vietnam (Phu Quoc Island, Bai Dai River Basin) This study
48 51 3m+45st/a Vietnam (Phu Quoc Island, Duong Dong River Basin) This study
C. striata 40 50 8m+2sm+2st+28a India Banerjee et al. 1988
40 48 8m+6st+26a India (Assam, Meghalaya) Dhar and Chatterjee 1984
40 48 8m+2st+30a India (Imphal) Rishi and Haobam 1990
40 50 8m+2sm+30st/a India (WB) Manna and Prasad 1973
40 58 8m+10sm+22a India (Manipur) Sobita and Bhagirath 2006
40 48 6m+2sm+10st+22a Northeastern India Kumar et al. 2013; 2019
42 48 6m+36st/a Northeast Thailand (Khon Kaen, Mahasakam) Supiwong et al. 2009
44 46 2m+42a China Wu et al. 1994
43 50 7m/sm/st+36a Northeastern Thailand (Chi Basin) Cioffi et al. 2015
44 50 6m/sm/st+38a Central and Southern Thailand (Chao Phraya Basin, Tapi Basin) Cioffi et al. 2015
44 48 2m+2sm+40a Thailand Donsakul and Magtoon 1991
44 48 2m+2sm+40st/a Vietnam (Phu Quoc Island, Bai Dai River Basin) This study
43 48 3m+2sm+38st/a This study

The probable maximum age of isolation of Phu Quoc Island from the Cambodian mainland is about ten thousand years when sea levels rose after the end of the last glacial period (Kuznetsov and Kuznetsova 2011). The short-term isolation of the island populations of C. lucius and C. striata probably contributed either to the appearance of chromosomal polymorphism or its maintenance due to at least two types of chromosomal rearrangements. Further cytogenetic studies of these two snakehead species from different river basins of the island and an increase in the sample size will most likely reveal an even greater range of variability in the number of 2n and NF.

Karyotypic evolution in Channa species

An analysis of cytogenetic data (Dhar and Chatterjee 1984; Banerjee et al. 1988; Rishi and Haobam 1990; Sobita and Bhagirath 2006; Tanomtong et al. 2014; Cioffi et al. 2015; Kumar et al. 2019) indicated that the karyotypic evolution in Channa species occurred in different directions and at different rates, which led to a wide chromosomal diversity from 2n = 32 to 2n = 112 (Fig. 3). The proportion of biarmed chromosomes in the karyotype varies widely from 0% to 100%. The regression between the proportion of biarmed chromosomes in the karyotype and the diploid number is y = -0.0046x + 0.551 (R² = 0.102), and the Spearman correlation is Rs = -0.28 (Fig. 4). The weak correlation between the two variables (2n and proportion of m/sm chromosomes) is apparently due to chromosomal rearrangements that affected the trends of karyotypic evolution in the genus Channa.

Figure 3.

Phylogenetic tree of the cytogenetically studied Channa species (based on Kumar et al. 2019 with modifications and additions) indicating the putative ancestral karyotype (in a rectangle) and range of variability of diploid chromosome numbers.

Figure 4.

Scatter plot of a diploid chromosome number and proportion of metacentric/submetacentric chromosomes (m/sm) with overall regression line for the genus Channa. The diameter and color of a circle indicate the number of species from 1 to 2.

The probable ancestral karyotype of snakeheads consisted of 48 uniarmed chromosomes, which would require a minimum number of chromosome rearrangements during the karyotype transformation of the number of Channa species. Among the cytogenetically studied species, C. argus and C. lucius have a karyotype with 2n = 48; the latter is also characterized by plesiomorphic features, such as the gular scales, which is absent in most species of Asian snakeheads (Li et al. 2006). The main trend of karyotypic evolution of Channa species is associated with a decrease in the number of chromosomes due to centric fusions (Robertsonian translocations) and an increase in the number of chromosome arms due to pericentric inversions (Fig. 5). As a result, in some populations of C. punctata, symmetrical karyotypes with 2n = 32 appeared, consisting exclusively of biarmed chromosomes (Dhar and Chatterjee 1984; Rakshit et al. 2015). Another direction of karyotype transformation is associated with an increase in the number of chromosomes as a result of centric fission and polyploidization, followed by an increase in the proportion of m/sm chromosomes as a result of centric fusions and pericentric inversions (Fig. 5). The huge variability in 2n of C. gachua (2n = 52–112), C. orientalis (2n = 52–78), and C. stewartii (2n = 66–104) indicates both the possibility of ploidy change in different populations (Cioffi et al. 2015; Kumar et al. 2019) and their co-evolution (Tanomtong et al. 2014). An additional direction of karyotype transformation in Channa is associated with an increase in the proportion of biarmed chromosomes without changing 2n (mainly due to pericentric inversions) (Fig. 5). It is likely that another mechanism, such as centromere repositioning, could also be involved in alterations in chromosome morphology (Sobita and Bhagirath 2006; Rakshit et al. 2015).

Figure 5.

The trends of karyotypic evolution for the genus Channa. The thick blue arrow shows the most likely main trend in karyotypic evolution. The lower rectangle shows the ancestral karyotype. fu – centric fusions, fiss – centric fissions, per inv – pericentric inversions, polypl – polyploidization.

Chromosomal rearrangements, which involve karyotypic structural changes such as inversions and fusions, may play an important role in the adaptive evolution of fish (Wellband et al. 2019; Cayuela et al. 2020). Rearrangements disturb homologous chromosome pairing during meiosis, resulting in tight linkage among genes encoding adaptations (for example, to salinity gradient and temperature) within rearranged regions (Barth et al. 2017; Wellenreuther and Bernatchez 2018). Such chromosomal rearrangements suppress recombination, and important functional genes are inherited together, which may contribute to adaptive population divergence (Barth et al. 2017). C. gachua is known to be well adapted to survive in a variety of habitats, in higher mountain areas with fluctuating climates, and has more resistance than other Channa species (Courtenay and Williams 2004; Tanomtong et al. 2014), which may be due to the high level of karyotypic variability.

The proposed expansion of the ranges of modern taxa of Asian snakeheads at the Miocene/Pliocene boundary, combined with climatic fluctuations, led to repeated isolations of populations, especially continental islands, and secondary contacts between them (Adamson et al. 2010; Tan et al. 2012; Wang et al. 2021), which probably influenced the chromosome diversification in the genus Channa with the formation of intrapopulation and interpopulation chromosomal variability. The karyotypic diversity of snakeheads can also increase as a result of hybridization, which is possible even between species that differ in the number of 2n and NF (Ou et al. 2018). Obviously, further studies will make it possible to reveal even greater karyotypic diversity associated with the appearance of biarmed chromosomes within the framework of the main trend in the karyotypic evolution of Channa species.

Acknowledgements

I am grateful to the administration of the Vietnam-Russian Tropical Center for organizing and financially supporting field research on Phu Quoc Island.

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ORCID

Denis V. Prazdnikov https://orcid.org/0000-0003-0447-7431

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