Karyotype analysis of Euphorbia hirta (2n = 18). Standard staining of mitotic interphase nucleus (A); standard staining of mitotic metaphase (B); silver impregnation of mitotic interphase nucleus (C); fluorochrome banding of metaphase chromosomes stained with CMA (D) and DAPI (E) and superposed images (F); C-banding of chromosomes stained with CMA/DAPI (C-CMA/DAPI; G–H); and metaphase chromosomes hybridized with 5S (red) and 45S (green) rDNA probes (I). Arrows and arrowheads indicate 5S and 45S rDNA sites, respectively.

  Part of: Santana KCB, Pinangé DSB, Vasconcelos S, Oliveira AR, Brasileiro-Vidal AC, Alves MV, Benko-Iseppon AM (2016) Unraveling the karyotype structure of the spurges Euphorbia hirta Linnaeus, 1753 and E. hyssopifolia Linnaeus, 1753 (Euphorbiaceae) using genome size estimation and heterochromatin differentiation. Comparative Cytogenetics 10(4): 657-669. https://doi.org/10.3897/CompCytogen.v10i4.8193