2026-10-11T18:10:12Z https://compcytogen.pensoft.net/oai.php

10.3897/compcytogen.v3i1.2 2009-08-06 compcytogen

Bulgarian Academy of Sciences, Bulgaria author Chassovnikarova, T Bulgarian Academy of Sciences, Bulgaria author Atanasov, Nasko Plovdiv University, Faculty of Biology, Bulgaria author Dimitrov, H 2009-08-06 2009-08-06 2009 Comparative Cytogenetics 1993-078X 1993-0771 3 1 1 9 2009 10.3897/compcytogen.v3i1.2 https://compcytogen.pensoft.net/article/1670/ https://compcytogen.pensoft.net/article/1670/download/pdf/ Chromosome polymorphism in Bulgarian populations of the striped field mouse (Apodemus agrarius Pallas, 1771) has been described. The diploid chromosome number is 2n=48 (NFa = 54). In the karyotype of 3 specimens from the Iskar region, the presence of an additional B chromosome has been established for the first time. The autosomes are 19 acrocentric pairs, continuously decreasing in size, and 4 pairs of bi-armed chromosomes, barely distinguishable by size and location of the centromere. Specimens with 3 pairs of metacentric chromosomes were firstly described in Bulgaria for the regions of Iskar and Omurtag. The localization of heterochromatin in the centromeric regions of the chromosomes, blocks of heterochromatin of different sizes, as well as intercalated bands, distinguishable in weakly spiraled chromosomes are found. Telomeric heterochromatin is present in the largest autosomal pair and in two of the middle-sized autosomal pairs. The largest and smallest pairs of be-armed chromosomes do not have centromeric heterochromatin, whereas all the other autosomal pairs do. The presence of a NOR in 6 chromosomal pairs is established. Two of the pairs exhibited pericentromeric NORs, whereas the other 4 displayed telomeric NORs. The karyotype analysis illustrates the chromosome and genome polymorphism of A. agrarius in Bulgarian populations. text/html en_US Pensoft Publishers Apodemus agrarius karyotype chromosomal polymorphism B chromosomes Chromosome polymorphism in Bulgarian populations of the striped field mouse (Apodemus agrarius Pallas 1771) Research Article

10.3897/compcytogen.v3i1.4 2009-08-06 compcytogen

National Academy of Sciences, Yerevan, Armenia author Martirosyan, A National Academy of Sciences, Yerevan, Armenia author Stepanyan, Ilona 2009-08-06 2009-08-06 2009 Comparative Cytogenetics 1993-078X 1993-0771 3 1 11 24 2009 10.3897/compcytogen.v3i1.4 https://compcytogen.pensoft.net/article/1671/ https://compcytogen.pensoft.net/article/1671/download/pdf/ Сhromosomal complements of Pelophylax ridibundus Pallas, 1771 from 9 localities (Northern, Central and South Armenia) and Rana macrocnemis Boulenger, 1885 from one locality (North-West Armenia) have been analyzed. The chromosome sets of P. ridibundus collected from 8 localities showed 2n=26, (10m+12sm+4st; NF=52). A secondary constriction has been observed in all studied individuals on the 10-th chromosome pair showing NOR-positive reaction. C-positive heterochromatin blocks have been observed on long arms of the 2-nd and 10-th pairs of chromosomes (7 localities). In addition, C-heterochromatin blocks have been found on interstitial regions of short arms of the 12-th pairs, as well as in telomeric regions of long arms of the 9-th pairs and on short arms of the 5-th pair in the frogs from 2 localities. The karyotype of P. ridibundus from populations near Ejmiatsin differs from other populations (2n=26, 12m+10sm+4st). Diploid number of chromosomes of R. macrocnemis was also 26 (8m+12sm+6st, NF=52). Blocks of C-positive heterochromatin have been revealed in telomeric parts of the 1-st, 2-nd (p), 3-rd (q), 4-th (q), 6-th, 9-th (p), 10-th (p,q) and 13-th (q) pairs, as well as in interstitial regions of the 1-st and 2-nd pairs of chromosomes.  Intrapopulation and interpopulation geographic variations of karyotypes and C-heterochromatin banding patterns of P. ridibundus have been revealed. Karyotypically, morphotypes “macrocnemis” and “camerani” are closely related. text/html en_US Pensoft Publishers Amphibia Ranidae Pelophylax Rana karyotypes C-positive heterochromatin blocks chromosome polymorphism Features of the karyotypes of Pelophylax ridibundus Pallas, 1771 and Rana macrocnemis Boulenger, 1885 (Amphibia: Ranidae) from Armenia Research Article

10.3897/compcytogen.v3i1.5 2009-08-06 compcytogen

Saratov State Medical University, Saratov, author Durnova, N 2009-08-06 2009-08-06 2009 Comparative Cytogenetics 1993-078X 1993-0771 3 1 25 31 2009 10.3897/compcytogen.v3i1.5 https://compcytogen.pensoft.net/article/1672/ https://compcytogen.pensoft.net/article/1672/download/pdf/ Karyotypic characteristics of the chironomids Demeijerea rufipes (Linnaeus, 1761) and Xenochironomus sp., obligatory miners of freshwater sponges, are considered. The karyotype of Xenochironomus sp. is described for the first time. It is assumed that the ordered spatial organization of chromosomes of these species, expressed in terms of the presence of chromocentres of different degrees of stability and an almost complete absence of polymorphism for chromosome rearrangements, represents an adaptation to inhabiting host tissues, i.e. to habitat relative constancy. text/html en_US Pensoft Publishers Xenochironomus Demeijerea karyotype chromocentre Chromosome analysis of chironomids (Diptera: Chironomidae) – obligatory miners of freshwater sponges (Porifera) Research Article

10.3897/compcytogen.v3i1.6 2009-08-06 compcytogen

Russian Academy of Sciences, St. Petersburg, author Golub, N University of Turku, Turku, Finland author Nokkala, Seppo 2009-08-06 2009-08-06 2009 Comparative Cytogenetics 1993-078X 1993-0771 3 1 33 41 2009 10.3897/compcytogen.v3i1.6 https://compcytogen.pensoft.net/article/1673/ https://compcytogen.pensoft.net/article/1673/download/pdf/ Karyotypes of eight Psocoptera species are reported for the first time. In Valenzuela oyamai (Enderlein, 1906) (Caeciliusidae), Peripsocus golubae Lienhard, 2006 (Peripsocidae), Trichopsocus dalii (McLachlan, 1867) (Trichopsocidae), Hemineura dispar Tetens, 1891 (Elipsocidae), and Amphigerontia contaminata (Stephens, 1836) (Psocidae) 2n = 16 + XX/X0. In Elipsocus moebiusi Tetens, 1891 (Elipsocidae) 2n = 12 + XX/X0. Neopsocopsis hitricornis (Reuter, 1893) (Psocidae) has 2n = 14 + XX/X0, and Kolbia quisquiliarum Bertkau, 1883 (Amphipsocidae) has 2n = 14 + neo-XY/XX. In addition, three seminal follicles per testis have been established in V. oyamai, H. dispar, A. contaminata, and N. hitricornis and one follicle in P. golubae and T. dalii. All the data available on psocopteran karyotypes are tabulated and shortly reviewed. text/html en_US Pensoft Publishers Psocoptera karyotype chromosome number chromosomal mechanism of sex determination Chromosome numbers in eight species of Palaearctic Psocoptera (Insecta) Research Article

10.3897/compcytogen.v3i1.7 2009-08-06 compcytogen

Punjabi University, Patiala, India author Kaur, Harbhajan Dr.Y.S.Parmar University of Horticulture and Forestry, Solan, India author Suman, Vikas 2009-08-06 2009-08-06 2009 Comparative Cytogenetics 1993-078X 1993-0771 3 1 43 50 2009 10.3897/compcytogen.v3i1.7 https://compcytogen.pensoft.net/article/1674/ https://compcytogen.pensoft.net/article/1674/download/pdf/ The Lygaeidae (Heteroptera) are a large and diverse family in which the male diploid chromosomal complement ranges from 10 to 30. Diploid numbers of 14 and 16 are taken as two modal numbers of the family. The Rhyparochrominae, one of the largest subfamilies of the Lygaeidae, are known to be heterogeneous both cytologically and morphologically. Available data on the tribe Rhyparochromini reveal that all species are characterized by the presence of a pair of microchromosomes (m-chromosomes) and have an XY/XX (♂/♀) sex chromosome determining system. Dieuches coloratus (Distant, 1909) and D. insignis (Distant, 1918) belonging to Rhyparochromini, have 2n=14=10A+2m+XY and 2n=12=8A+2m+XY respectively. Both the species are similar inone pair of distinctly large autosomes in their chromosome complements. The metaphase plate arrangement of autosomes, sex chromosomes and m-chromosomes in D. coloratus is similar to the common condition observed in the tribe Rhyparochromini. In D. insignis, however, the arrangement is different. Here, metaphase I is usual in showing peripheral position of autosomes and central position of sex chromosomes and m-chromosomes. At metaphase II, however, autosomes, sex chromosomes and m-chromosomes are peripherally placed, an arrangement, which is not reported earlier in the tribe Rhyparochromini. text/html en_US Pensoft Publishers Lygaeidae Rhyparochromini Dieuches chromosomes meiosis metaphase plate arrangement Chromosomes and their meiotic behaviour in two species of Dieuches Dohrn, 1860 (Heteroptera: Lygaeidae: Rhyparochromini) Research Article

10.3897/compcytogen.v3i1.8 2009-08-06 compcytogen

C.C.S. University, Meerut, India author Malik, Anjali C.C.S. University, Meerut, India author Srivastava, A 2009-08-06 2009-08-06 2009 Comparative Cytogenetics 1993-078X 1993-0771 3 1 51 55 2009 10.3897/compcytogen.v3i1.8 https://compcytogen.pensoft.net/article/1675/ https://compcytogen.pensoft.net/article/1675/download/pdf/ Somatic chromosomes of Carthamus tinctorius L. were analysed. A karyotype formula for each studied population was formulated. Although all samples have 2n = 24, they could be differentiated by their karyotype formula and quantitative parameters of the karyotypes. The chromosomes were assorted into different categories on the basis of arms ratio following Levan et al. (1964). These were further subdivided into different types, on the basis of total length of the chromosomes. Based on an evolutionary point of view, variation in total chromosome length without major changes in the karyotype formula suggests that changes in the amounts of genomic DNA are proportional to the relative length of an each chromosome arm. All samples possessed symmetrical or slightly asymmetrical karyotype. The samples belonging to the same species were showing intra-specific or inter-specific chromosome polymorphism. This finding may provide useful information for Carthamus evolutionary, genetic, and breeding studies. text/html en_US Pensoft Publishers Asteraceae Carthamus karyotype variation Karyotypic analysis of different populations of Carthamus tinctorius Linnaeus (Asteraceae) Research Article

10.3897/compcytogen.v3i1.9 2009-08-06 compcytogen

A.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow, author Bulatova, N University of York, York, United Kingdom author Searle, J A.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow, author Nadjafova, R A.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow, author Pavlova, Svetlana V. https://orcid.org/0000-0001-7823-9618 Penza State Pedagogical University, Penza, author Bystrakova, N 2009-08-06 2009-08-06 2009 Comparative Cytogenetics 1993-078X 1993-0771 3 1 57 62 2009 10.3897/compcytogen.v3i1.9 https://compcytogen.pensoft.net/article/1676/ https://compcytogen.pensoft.net/article/1676/download/pdf/ For many decades karyotype was the only source of overall genomic information obtained from species of mammal. However, approaches have been developed in recent years to obtain molecular and ultimately genomic information directly from the extracted DNA of an organism. Molecular data have accumulated hugely for mammalian taxa. The growing volume of studies should motivate field researchers to collect suitable samples for molecular analysis from various species across all their ranges. This is the reason why we here include a molecular sampling procedure within a field work protocol, which also includes more traditional (including cytogenetic) techniques. In this way we hope to foster the development of molecular and genomic studies in non-standard wild mammals. text/html en_US Pensoft Publishers mammalian genome molecular phylogeography field studies molecular sampling procedure karyotype Sorex araneus Field protocols for the genomic era Research Article

10.3897/compcytogen.v3i1.1 2009-08-06 compcytogen

Moscow State University, Moscow, Russia author Gokhman, Vladimir https://orcid.org/0000-0001-9909-7559 National Academy of Sciences of Ukraine, Ukraine author Gumovsky, Alex 2009-08-06 2009-08-06 2009 Comparative Cytogenetics 1993-078X 1993-0771 3 1 63 69 2009 10.3897/compcytogen.v3i1.1 https://compcytogen.pensoft.net/article/1677/ https://compcytogen.pensoft.net/article/1677/download/pdf/ An overview of karyotype evolution in the superfamily Chalcidoidea is given. Structural types of chromosome sets in the superfamily are listed. Main pathways of karyotypic change in the Chalcidoidea are outlined. The chromosome set containing eleven subtelo- or acrocentrics is considered as an ancestral karyotype for the superfamily. Multiple independent reductions in n values through chromosomal fusions presumably occurred in various groups of chalcid families. text/html en_US Pensoft Publishers chromosomes karyotypes parasitic wasps Hymenoptera Chalcidoidea Main trends of karyotype evolution in the superfamily Chalcidoidea (Hymenoptera) Research Article

10.3897/compcytogen.v3i1.10 2009-08-06 compcytogen

Unaffiliated, author Vavilov, N 2009-08-06 2009-08-06 2009 Comparative Cytogenetics 1993-078X 1993-0771 3 1 71 78 2009 10.3897/compcytogen.v3i1.10 https://compcytogen.pensoft.net/article/1678/ https://compcytogen.pensoft.net/article/1678/download/pdf/ Archives of Russian cytogenetics keep many-sided evidences of close interactions of chromosome studies with the fundamental genetics. Here we present one of the most exciting documents of the initial existence of such connection. It stressed, in particular, the strong practical motivation for development of wide botanical chromosomal studies under influence of global genetic approach built by Nikolai Vavilov. He was who brought together the famous national cytogeneticists in his institute in Leningrad (now again St. Petersburg), and it is demonstrated already in the first issue of the profile journal he inherited from former applied plant breeders. In 1927, a special number of the new-renamed “Bulletin of Applied Botany, of Genetics and Plant-Breeding” (former “Bulletin of Applied Botany and Plant-Breeding”, 1927: vol. 17, No.3) has been dedicated “to the founder of genetical cytology in our country”, Sergei G. Navashin. The volume included the papers under such names as G. Levitsky, I. Sveshnikova, H. Emme, M. Navashin, V. Rybin and G. Karpechenko and was completed by a small publication of N. Vavilov himself. This communication given as “preliminary” is important in many respects, drawing the far going idea for the genetic studies on the Linnean species (either in plants or among animals) and running right up to analyses of chromosome and genomic polymorphisms or polytypy activated far long ago after Vavilov’s epoch. text/html en_US Pensoft Publishers fundamental genetics gene geography Linnean species origin of cultivated plants history of science Geographical regularities in the distribution of the genes of cultivated plants Research Article

10.3897/compcytogen.v3i1.11 2009-08-06 compcytogen

Comparative Cytogenetics Editorial Board, author Editorial Board, Comparative Cytogenetics 2009-08-06 2009-08-06 2009 Comparative Cytogenetics 1993-078X 1993-0771 3 1 79 81 2009 10.3897/compcytogen.v3i1.11 https://compcytogen.pensoft.net/article/1679/ https://compcytogen.pensoft.net/article/1679/download/pdf/ On 6 December 2008 Egeny Kachvoryan, an eminent specialist in the field of entomology, taxonomy, cytogenetics and ecology of blackflies (Simulidae, Diptera) passed away. She had many ideas and plans for the future and until her last day she was struggling courageously with the incurable disease. text/html en_US Pensoft Publishers Egeny Kachvoryan Doctor Egeny Kachvoryan (2.III.1942-6.XII.2008) In Memoriam

10.3897/compcytogen.v3i1.12 2009-08-06 compcytogen

Russian Academy of Sciences, author Goldenkova-Pavlova, Irina 2009-08-06 2009-08-06 2009 Comparative Cytogenetics 1993-078X 1993-0771 3 1 83 84 2009 10.3897/compcytogen.v3i1.12 https://compcytogen.pensoft.net/article/1680/ https://compcytogen.pensoft.net/article/1680/download/pdf/ From 22 June to 28 June 2009 the Fifth Congress of the Vavilov Society of Geneticists and Selectionists at the Russian Academy of Sciences was held in Moscow. The congress was dedicated to the 200th anniversary of Charles Darwin. text/html en_US Pensoft Publishers informational note Informational note Correspondence

10.3897/compcytogen.v3i2.14 2009-12-29 compcytogen

Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia author Polyakov, Andrei Kemerovo State University, Kemerovo, author Ilyashenko, V Kemerovo State University, Kemerovo, author Onischenko, S Siberian Branch of Russian Academy of Sciences, Novosibirsk, author Panov, V Siberian Branch of Russian Academy of Sciences, Novosibirsk, author Borodin, Pavel https://orcid.org/0000-0002-6717-844X 2009-12-29 2009-12-29 2009 Comparative Cytogenetics 1993-078X 1993-0771 3 85 89 2009 10.3897/compcytogen.v3i2.14 https://compcytogen.pensoft.net/article/1681/ https://compcytogen.pensoft.net/article/1681/download/pdf/ Genetic diversity between of the Novosibirsk and Tomsk chromosome races of the common shrew (Sorex araneus) was analyzed using 39 polymorphic AFLP (amplified fragments length polymorphism) markers. Exact and F-statistics tests for population differentiation demonstrated significant interracial difference in allele frequencies and significant subdivision between the races. The value of the genetic distance between the chromosome races observed in this study corresponds to that found between subspecies of mammals studied so far. text/html en_US Pensoft Publishers Sorex araneus chromosome races AFLP interracial diversity AFLP diversity between the Novosibirsk and Tomsk chromosome races of the common shrew (Sorex araneus) Research Article

10.3897/compcytogen.v3i2.15 2009-12-29 compcytogen

Royal holloway, University of London, United Kingdom author Angus, Robert https://orcid.org/https://orcid.org/0000-0002-3860-5617 2009-12-29 2009-12-29 2009 Comparative Cytogenetics 1993-078X 1993-0771 3 91 96 2009 10.3897/compcytogen.v3i2.15 https://compcytogen.pensoft.net/article/1682/ https://compcytogen.pensoft.net/article/1682/download/pdf/ Aphodius abdominalis Bonelli, 1812 is shown to have a karyotype comprising nine pairs of autosomes and sex chromosomes which are X0 (male), XX (female). At first metaphase of meiosis the X chromosome is linked to an autosomal bivalent by a darkly staining area of the cytoplasm, resembling the Xy p arrangement typical of Aphodius species, but giving nine, rather than 10, elements in the nucleus. C-banding, which shows the centromeres, confirms this unusual arrangement. A. bonvouloiri, the only other known species of subgenus Agolius Mulsant et Rey, 1869, has a male karyotype with nine pairs of autosomes and Xy sex chromosomes. No preparations of its meiosis are available. text/html en_US Pensoft Publishers Aphodius Agolius karyotypes sex chromosomes Xyp X0 C-banding X-autosomal trivalent Unusual arrangement and behaviour of the sex chromosomes of Aphodius (Agolius) abdominalis Bonelli, 1812, and comparison with A. (A.) bonvouloiri Harold, 1860 (Coleoptera: Aphodiidae) Research Article

10.3897/compcytogen.v3i2.16 2009-12-29 compcytogen

Universidade Federal de Viçosa, Viçosa, Brazil author Barros, Luísa https://orcid.org/0000-0002-1501-4734 Laboratório de Mirmecologia, Ilheus, Brazil author Mariano, Cléa Universidade Federal de Viçosa, Viçosa, Brazil author Pompolo, S Universidade Estadual de Santa Cruz, Ilhéus, Brazil author Delabie, Jacques https://orcid.org/0000-0002-2695-1061 2009-12-29 2009-12-29 2009 Comparative Cytogenetics 1993-078X 1993-0771 3 97 102 2009 10.3897/compcytogen.v3i2.16 https://compcytogen.pensoft.net/article/1683/ https://compcytogen.pensoft.net/article/1683/download/pdf/ The distribution of the threatened ant Dinoponera lucida is limited to the south of the State of Bahia, farthest east of Minas Gerais and north of Espirito Santo, Brazil. Recent cytogenetic studies carried out in 15 sites distributed along the range of the ant indicated high chromosome number variation in the populations of Bahia, 2n=106 to 120 chromosomes, while the populations of Espirito Santo presented a constant number, with 2n=118 chromosomes. This study aimed at describing the banding pattern of D. lucida chromosomes, in some populations of Espirito Santo and Bahia, applying the Hsc-FA technique (secondary constriction heterochromatin-associated bands by fluorescence using acridine) and identifying the nucleolar organizing region (active NORs) impregnated with AgNO 3 which is especially important for the cytogenetic characterization of a species. The utilization of these two techniques showed positive interstitial markings on the region of the long arm of the pair AMt, similar to those obtained with the CMA 3 fluorochrome and with the FISH technique complementing the cytogenetic data of this species. text/html en_US Pensoft Publishers cytogenetics simple NOR fluorochrome ant Formicidae Hsc-FA and NOR bandings on chromosomes of the giant ant Dinoponera lucida Emery, 1901 (Hymenoptera: Formicidae) Research Article

10.3897/compcytogen.v3i2.17 2009-12-29 compcytogen

Centro Federal de Educação Tecnológica – CEFET, Tucuruí-Pará, Brazil author De Souza, A Universidade Federal do Pará, Belém-Pará, Brazil author Nagamachi, C Universidade Federal do Pará, Belém – Pará, Brazil author Milhomem, Susana Instituto Nacional de Pesquisas da Amazônia, Manaus, Brazil author Feldberg, Eliana https://orcid.org/0000-0002-2962-4541 Universidade Federal do Pará, Belém – Pará, Brazil author Pieczarka, J 2009-12-29 2009-12-29 2009 Comparative Cytogenetics 1993-078X 1993-0771 3 103 109 2009 10.3897/compcytogen.v3i2.17 https://compcytogen.pensoft.net/article/1684/ https://compcytogen.pensoft.net/article/1684/download/pdf/ This study describes the karyotypes of three species of the genus Peckoltia (Loricariidae: Ancistrini). Fishes were collected in the Jari (Peckoltia sp. 1 and Peckoltia sp. 2) and Xingu rivers (Peckoltia vittata (Steindachner, 1881)) in the Amazon rainforest. Karyotypes were 2n = 52 for Peckoltia vittata (FN=102: 16 metacentrics (m), 20 submetacentrics (sm), 14 subtelocentrics (st), 2 acrocentrics (a)) and Peckoltia sp. 2 (FN=102: 32 m + sm, 18st, 2a). Peckoltia sp. 1 (FN=102: 44 m + sm, 6st, 2a, 1B) had 2n = 53 due to a B chromosome. The species differ in chromosomal morphology. Nucleolar Organizer Regions (NOR) was identified within a distal region of the long arm of pair 9 in Peckoltia vittata, in pair 10 and in a homologue of pair 25 in Peckoltia sp. 1, as well as in pair 17 and in a homologue of pair 18 in Peckoltia sp. 2. Chromomycin A3 banding agreed with the location of the NORs. C-banding patterns revealed large non-centromeric heterochromatic blocks, probably of a common origin. Our results suggest a higher level of similarity between Peckoltia vittata and Peckoltia sp. 1. text/html en_US Pensoft Publishers phylogeny fish Loricariidae cytogenetics C-banding NOR Cytogenetic analysis in catfish species of the genus Peckoltia Miranda Ribeiro, 1912 (Teleostei: Siluriformes: Loricariidae) Research Article

10.3897/compcytogen.v3i2.18 2009-12-29 compcytogen

Zoological Institute, St. Petersburg, St. Petersburg, author Kuznetsova, Valentina G. https://orcid.org/0000-0001-8386-5453 Polish Academy of Sciences, Kraków, Poland author Maryańska-Nadachowska, Anna University of Turku, Turku, Finland author Nokkala, Seppo 2009-12-29 2009-12-29 2009 Comparative Cytogenetics 1993-078X 1993-0771 3 111 123 2009 10.3897/compcytogen.v3i2.18 https://compcytogen.pensoft.net/article/1685/ https://compcytogen.pensoft.net/article/1685/download/pdf/ Males of Hysteropterum albaceticum Dlabola, 1983 and Agalmatium bilobum (Fieber, 1877) display a chromosomal complement of 2n = 26 + X, which is a basic one of the tribe Issini (Issidae). In the present study, silver staining, C-banding and a base specific CMA3 -and DAPI-banding were used with the aim of identifying possible cytogenetic markers and distinguishing between karyotypes with the same chromosome number and no detectable inter-species differences in karyotype structure. We characterized the species studied in terms of the distribution and molecular structure of C-heterochromatin regions and the location of nucleolus organizing regions (NORs). The species are shown to differ considerably in the amount of heterochromatin, its distribution pattern along the karyotypes and its stain ability with DAPI and CMA3. text/html en_US Pensoft Publishers Hysteropterum albaceticum Agalmatium bilobum Homoptera Auchenorrhyncha Issidae NORs C-heterochromatin DAPI/CMA3 -staining chiasmata polymorphism Karyotype characterization of planthopper species Hysteropterum albaceticum Dlabola, 1983 and Agalmatium bilobum (Fieber, 1877) (Homoptera: Auchenorrhyncha: Issidae) using AgNOR-, C- and DAPI/CMA3 -banding techniques Research Article

10.3897/compcytogen.v3i2.19 2009-12-29 compcytogen

Bulgarian Academy of Sciences, Sofia, Bulgaria author Grozeva, Snejana https://orcid.org/0000-0003-3196-8068 University of Turku, Turku, Finland author Nokkala, Seppo Bulgarian Academy of Sciences, Sofia, Bulgaria author Simov, Nikolay https://orcid.org/0000-0003-1626-2964 2009-12-29 2009-12-29 2009 Comparative Cytogenetics 1993-078X 1993-0771 3 125 130 2009 10.3897/compcytogen.v3i2.19 https://compcytogen.pensoft.net/article/1686/ https://compcytogen.pensoft.net/article/1686/download/pdf/ The type of male meiosis is a stable character at the family level in the order Heteroptera and provides additional information on the relationships between taxa. The most common pattern, probably ancestral in the order is chiasmate meiosis; however achiasmate meiosis has been described in five families of terrestrial Heteroptera, all belonging to the infraorder Cimicomorpha (Anthocoridae, Microphysidae, Cimicidae, Miridae, Nabidae). Among water bugs, achiasmate meiosis is reported in the families Saldidae (Leptopodomorpha) and Мicronectidae (Nepomorpha). Regarding the third infraorder of water bugs, Gerromorpha, data on meiotic patterns are absent, except for the Limnogonus aduncus Drake, Harris, 1933 (Gerridae) possessing chiasmate meiosis. In this paper, the male meiotic pattern of six water bugs species from infraorders Nepomorpha (Plea minutissima minnutissima Leach, 1817) and Gerromorpha (Mesovelia furcata Mulsant, Ray, 1852, Microvelia reticulata (Burmeister, 1835), Gerris costae fieberi Stichel, 1938, Hydrometra gracilenta Horváth, 1899, Velia pelagonensis Hoberlandt, 1941) is studied, and the karyotypes of the last two species are described for the first time. In the species examined, bivalents are chiasmate, so all these species possess chiasmate meiosis in males. text/html en_US Pensoft Publishers water Heteroptera holokinetic chromosomes chiasmate/achiasmate male meiosis Chiasmate male meiosis in six species of water bugs from infraorders Nepomorpha and Gerromorpha (Insecta: Heteroptera) Research Article

10.3897/compcytogen.v3i2.20 2009-12-29 compcytogen

Ivan Franko Zhytomyr State University, Zhytomyr, Ukraine author Garbar, A Ivan Franko Zhytomyr State University, Zhytomyr, Ukraine author Onyschuk, I Schmalhausen Institute of Zoology NAS of Ukraine, Kiev, Ukraine author Меzhzherin, S 2009-12-29 2009-12-29 2009 Comparative Cytogenetics 1993-078X 1993-0771 3 131 141 2009 10.3897/compcytogen.v3i2.20 https://compcytogen.pensoft.net/article/1687/ https://compcytogen.pensoft.net/article/1687/download/pdf/ Kariological investigation of the earthworm Octodrilus transpadanus (Rosa, 1884) in the Ukraine showed that this species is represented by at least three races of different ploidy with the basic chromosome number n=15. Strict geographic heterogeneity of electrophoretic spectra of different populations was registered: south-eastern populations (7n=105) are characterized by a clonal structure, whereas western (from 3n = 45 to 4n = 60) and south-western (2n=30) ones have a high level of polymorphism. The Crimea sample (4n=60) is well isolated, has no clonal structure and is characterized by a series of specific alleles , which are absent in forms from continental Ukraine. All the forms turned out to be well isolated morphologically, except for the clonal forms, which are very much alike. text/html en_US Pensoft Publishers earthworms Octolasion Octodrilus polyploidy genetic structure morphology Polyploid races, genetic structure and morphological features of the earthworm Octodrіlus transpadanus (Rosa, 1884) (Oligohaeta: Lumbricidae) in the Ukraine Research Article

10.3897/compcytogen.v4i1.21 2010-07-09 compcytogen

St. Petersburg State University, St. Petersburg, author Vershinina, Alisa Zoological Institute of Russian Academy of Science, St. Petersburg, Russia author Lukhtanov, Vladimir https://orcid.org/0000-0003-2856-2075 2010-07-09 2010-07-09 2010 Comparative Cytogenetics 1993-078X 1993-0771 4 1 1 11 2010 10.3897/compcytogen.v4i1.21 https://compcytogen.pensoft.net/article/1688/ https://compcytogen.pensoft.net/article/1688/download/pdf/ Agrodiaetus alcestis (Zerny, 1932) and A. demavendi (Pfeiffer, 1938) belong to the “brown” complex of the genus Agrodiaetus Hübner, 1822. This complex includes several cryptic species which are extremely uniform in wing colouration and genitalia structure, but have distinct chromosome numbers. In this paper we analyse karyotypes of A. alcestis karacetinae Lukhtanov et Dantchenko, 2002 and A. demavendi in populations from Iran. We demonstrate that A. alcestis karacetinae and A. demavendi are sympatric in the provinces Esfahan, Lorestan, Hamadan, Kurdestan, Kermanshah, and Markazi. The haploid chromosome number of A. alcestis karacetinae is found to be n=19 in all the populations studied. The karyotype of A. demavendi is not stable. The lowest chromosome numbers n=63-67 is observed in the south of the revealed distribution range (provinces Esfahan and Lorestan). The highest chromosome numbers (n=73-74) is found in Northwestern Iran in provinces Kurdestan and Zanjan. We also confirm that A. alcestis sensu lato appears as a polyphyletic taxon on the Bayesian phylogenetic tree inferred from the mitochondrial COI barcodes and should be most likely divided in two different species: A. alcestis sensu stricto and A. karacetinae. The new data on occurrence of A. admetus and A. ripartii in Iran are discussed. text/html en_US Pensoft Publishers Agrodiaetus butterfly chromosome COI DNA barcoding cryptic species Iran karyotype Lepidoptera Lycaenidae Geographical distribution of the cryptic species Agrodiaetus alcestis alcestis, A. alcestis karacetinae and A. demavendi (Lepidoptera: Lycaenidae) revealed by cytogenetic analysis Research Article

10.3897/compcytogen.v4i1.27 2010-07-09 compcytogen

Royal holloway, University of London, United Kingdom author Angus, Robert https://orcid.org/https://orcid.org/0000-0002-3860-5617 2010-07-09 2010-07-09 2010 Comparative Cytogenetics 1993-078X 1993-0771 4 1 13 20 2010 10.3897/compcytogen.v4i1.27 https://compcytogen.pensoft.net/article/1689/ https://compcytogen.pensoft.net/article/1689/download/pdf/ Karyological analysis has been carried out on Stictotarsus griseostriatus (De Geer, 1774) from Tjøme in southern Norway, one of the type localities of Deronectes maritimus Helliesen, 1890, as well as from the Canton of Valais in the Swiss Alps and Bavaria in the German Alps. True S. griseostriatus is shown to occur over much of the central Alps, and the synonymy of S. griseostriatus var. nigrescens Favre, 1890 is confirmed.  Analysis of Corsican material shows that the species inhabiting the island is S. ibericus Dutton et Angus, 2007. The known distributions of the S. griseostriatus group species occurring in the Alps and on Corsica are given, and Pleistocene fossil material from Starunia in the Ukrainian Carpathians is figured and discussed. A syntype of Potamodytes multilineatus Falkenström, 1922, in Helliesen’s collection in Oslo, is noted and a neotype designated for Deronectes maritimus Helliesen, 1890 as the original material on which the description was based cannot be found. text/html en_US Pensoft Publishers Stictotarsus griseostriatus S. ibericus Alps Corsica southern Norway Dytiscidae chromosomes karyotypes sibling species Pleistocene fossils neotype A third karyosystematic investigation of the Stictotarsus griseostriatus (De Geer) group of sibling species (Coleoptera: Dytiscidae) Research Article

10.3897/compcytogen.v4i1.29 2010-07-09 compcytogen

Moscow State University, Moscow, Russia author Gokhman, Vladimir https://orcid.org/0000-0001-9909-7559 2010-07-09 2010-07-09 2010 Comparative Cytogenetics 1993-078X 1993-0771 4 1 21 25 2010 10.3897/compcytogen.v4i1.29 https://compcytogen.pensoft.net/article/1690/ https://compcytogen.pensoft.net/article/1690/download/pdf/ Karyotypes of four species of the genus Metaphycus Mercet, 1917, namely, M. flavus (Howard, 1881) and M. luteolus (Timberlake, 1916) (both have n = 10 and 2n = 20), M. angustifrons Compere, 1957 (n = 9 and 2n = 18) and M. stanleyi Compere, 1940 (n = 5 and 2n = 10) were studied. The latter chromosome number, n = 5, is the lowest known one for the family Encyrtidae. A karyotype with n = 10 is considered ancestral for the genus Metaphycus. Karyotype evolution in this genus is likely to have occurred through chromosomal fusions. text/html en_US Pensoft Publishers chromosomes karyotypes Chalcidoidea Encyrtidae Metaphycus Chromosomes of parasitic wasps of the genus Metaphycus (Hymenoptera: Chalcidoidea: Encyrtidae) Research Article

10.3897/compcytogen.v4i1.23 2010-07-09 compcytogen

Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia author Kiknadze, Iya Siberian Branch of Russian Academy of Sciences, Novosibirsk, author Istomina, A 2010-07-09 2010-07-09 2010 Comparative Cytogenetics 1993-078X 1993-0771 4 1 27 43 2010 10.3897/compcytogen.v4i1.23 https://compcytogen.pensoft.net/article/1691/ https://compcytogen.pensoft.net/article/1691/download/pdf/ The karyotype structure and chromosomal polymorphism were studied in several European (European part of Russia and the Netherlands) and Asian (Siberia and Kazakhstan) populations of Chironomus luridus Strenzke, 1959. Inversion polymorphism was detected in six of the seven chromosome arms: three banding sequences detected in arm A, six sequences in arm B, two sequences in arm C, three sequences in arm E, five sequences in arm F, and two sequences in arm G. Only arm D was monomorphic in all studied populations. In total, 22 banding sequences were recorded in Ch. luridus; they form the banding sequence pool of this species. Thus, Ch. luridus can be regarded as a very polymorphic species. However, the European and Asian populations differed considerably in the levels of polymorphism: the Asian populations were less polymorphic, containing only 8 to 10 sequences, whereas the European populations had 11 to 16 sequences. The new banding sequences lurA3, lurB5, lurE3 were found in Asian populations, whereas the sequences lurB2, lurB3, lurB4, lurB6, lurE2, lurF1, lurF2a, lurF3, lurF4, and lurG2 аre lacked. The total level of inversion heterozygosity in the Asian populations was 12-25% versus 78-80% in the European populations. text/html en_US Pensoft Publishers karyotype banding sequences chromosomal polymorphism Chironomus luridus Karyotype and chromosomal polymorphism of Chironomus luridus Strenzke, 1959 (Diptera: Chironomidae) in European and Asian populations Research Article

10.3897/compcytogen.v4i1.31 2010-07-09 compcytogen

Universidade Federal do Pará, Belém – Pará, Brazil author Milhomem, Susana CNPq Scholarship on Scientific Initiation, Brazil, Brazil, Brazil author Castro, Roberta Universidade Federal do Pará, Belém-Pará, Brazil author Nagamachi, C Centro Federal de Educação Tecnológica – CEFET, Tucuruí-Pará, Brazil author De Souza, A Instituto Nacional de Pesquisas da Amazônia, Manaus, Brazil author Feldberg, Eliana https://orcid.org/0000-0002-2962-4541 Andar, Instituto de Ciências Biológicas, Universidade Federal do Pará, Pará, Brazil author Pieczarka, Julio 2010-07-09 2010-07-09 2010 Comparative Cytogenetics 1993-078X 1993-0771 4 1 45 54 2010 10.3897/compcytogen.v4i1.31 https://compcytogen.pensoft.net/article/1692/ https://compcytogen.pensoft.net/article/1692/download/pdf/ We analyzed the karyotypes of three specimens of fishes of the genus Hypostomus Lacepede, 1803 (Loricaridae) from Xingu River (Amazon region). We used conventional staining techniques, including C-banding, Ag-NOR staining, CMA3- and DAPI-banding. Each specimen had a different cytotype: Hypostomus sp. Xingu-1 had 2n=64 (32M/SM, 32ST/A); Hypostomus sp. Xingu-2 has 2n=66 (32M/SM, 34ST/A), and Hypostomus sp. Xingu-3 had 2n=65 (38M/SM, 26ST/A + 1 B). The three cytotypes showed similar C-, CMA3- and DAPI-banding patterns. The nucleolus organizing regions were located in the short arm of chromosome pair 25 of Hypostomus sp. Xingu-1 and pair 29 of Hypostomus sp. Xingu-2, and in the long arm of pair 30 of Hypostomus sp. Xingu-3, probably because of a pericentric inversion. A fusion/fission rearrangement explains the difference in the diploid number and number of M/SM and ST/A chromosomes between the 2n= 64 and 2n=66 cytotypes. The B chromosome most probably explains the difference between the 2n=64 and 2n=65 cytotypes. The cytotype with 2n=65 had a significantly larger number of M/SM chromosomes, probably because of pericentric inversions. These three cytotypes may represent different species. text/html en_US Pensoft Publishers Siluriformes Hypostomus cytogenetics heterochromatin variability C-banding Ag-NOR CMA3 and DAPI Different cytotypes in fishes of the genus Hypostomus Lcépède, 1803, (Siluriformes: Loricariidae) from Xingu river (Amazon region, Brazil) Research Article

10.3897/compcytogen.v4i1.25 2010-07-09 compcytogen

Russian Academy of Sciences, Moscow, author Kolomiets, Oxana L. Russian Academy of Sciences, Moscow, author Matveevsky, Sergey https://orcid.org/0000-0002-5808-7685 Russian Academy of Sciences, Moscow, Russia author Bakloushinskaya, Irina https://orcid.org/0000-0001-5914-1797 2010-07-09 2010-07-09 2010 Comparative Cytogenetics 1993-078X 1993-0771 4 1 55 66 2010 10.3897/compcytogen.v4i1.25 https://compcytogen.pensoft.net/article/1693/ https://compcytogen.pensoft.net/article/1693/download/pdf/ The synaptonemal complex (SC) surface-spreading technique was used to visualize the process of chromosome synapsis in spermatocytes and oocytes of E. talpinus Pallas, 1770, a species with the XX sex chromosome system in both males and females. We used electron microscopy and immunofluorescent localization of synaptonemal complex protein (SCP3) and centromeric proteins to analyze the structure and behaviour of synaptonemal complexes in prophase I of meiosis, aiming to reveal signs of meiotic sexual dimorphism in this species. We present evidence of considerable differences in the structure and behaviour of the axial structures of sex bivalents in male and female meiosis, despite the isomorphic G- and C-banding patterns of mitotic sex chromosomes. During meiotic prophase I, the sex bivalent in females behaved as autosomal bivalents, but it was not involved in the formation of the bouquet configuration or it was the first to leave it. The XX chromosomes of males formed closed sex bivalents. Only short tracts of SC were formed at both ends of the sex bivalent, while large middle segments of the lateral elements remained unpaired. The male sex chromosomes also formed characteristic “sex bodies”. In fact, electron microscopy revealed dense nucleolus-like bodies associated with unpaired parts of the axial elements. These regions of the sex chromosomes were poorly immunostained, because the distribution of SCP3 had a peculiar powder-like pattern, but SCP3 was not associated with the nucleolus-like bodies. We also revealed signs of sexual dimorphism in the dynamics of formation and destruction of autosomal SCs. In males, the total SC length was shorter than in females. The chromosome bouquet configuration was preserved up to the stage of early pachytene in females. The bouquet configuration in males was not expressed. At late pachytene, gaps were revealed in the structure of autosomal SCs in spermatocytes immunostained with antibodies to SCP3. The pattern of distribution of these gaps was comparable with the G-banding patterns of mitotic chromosomes. text/html en_US Pensoft Publishers sexual dimorphism meiosis synaptonemal complex male isomorphic sex chromosomes Ellobius talpinus variable position of centromeres Sexual dimorphism in prophase I of meiosis in the Northern mole vole (Ellobius talpinus Pallas, 1770) with isomorphic (XX) chromosomes in males and females Research Article

10.3897/compcytogen.v4i1.22 2010-07-09 compcytogen

Far East Branch of the Russian Academy of Sciences, Vladivostok, Russia author Kartavtseva, Irina https://orcid.org/0000-0003-2136-8253 Korea Maritime University, Busan, author Park, I-S 2010-07-09 2010-07-09 2010 Comparative Cytogenetics 1993-078X 1993-0771 4 1 67 71 2010 10.3897/compcytogen.v4i1.22 https://compcytogen.pensoft.net/article/1694/ https://compcytogen.pensoft.net/article/1694/download/pdf/ Cytogenetical examinations of Crocidura shantungensis Miller, 1901 from small Young Island of South Korea and the mainland of Russian Far East (Vladivostok) were carried out and literature data concerning Tsushima Island of Japan and Cheju Island of Korea were considered. The chromosome sets of all investigated specimens are characterized by 2n = 40 and NFa = 46. Four pairs of biarmed autosomes, 15 pairs of acrocentrics and two sex chromosomes were identified applying G- and C-banding. The X chromosome is a large metacentric whereas the Y chromosome is a middle sized acrocentric element. A variation in the Y chromosome size in samples from the three islands is noticeable. Comparative analysis of G-banded chromosomes of C. shantungensis (our material) and C. suaveolens (Pallas, 1811) (literature data) shows a similarity between karyotypes of these two species. text/html en_US Pensoft Publishers Crocidura shantungensis mammalian karyotype chromosomal polymorphism Y chromosome G-banding C-heterochromatin Y chromosome peculiarities and chromosomal G- and C-staining in Crocidura shantungensis Miller, 1901 (Soricomorpha: Soricidae) Research Article

10.3897/compcytogen.v4i1.30 2010-07-09 compcytogen

A.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow, author Pavlova, Svetlana V. https://orcid.org/0000-0001-7823-9618 2010-07-09 2010-07-09 2010 Comparative Cytogenetics 1993-078X 1993-0771 4 1 73 78 2010 10.3897/compcytogen.v4i1.30 https://compcytogen.pensoft.net/article/1695/ https://compcytogen.pensoft.net/article/1695/download/pdf/ A unique arm chromosome combination detected in the common shrew karyotype from a Karelian locality within the Arctic Circle (Kandalaksha State Nature Reserve, European Russia) is interpreted as a new chromosome race - Poyakonda. According to the standard nomenclature of G-banded chromosomes of Sorex araneus the karyotype contains the species specific bi-armed autosomes af, bc, jl, tu and the typical complex sex chromosome system (two metacentrics XX in the female and one metacentric/two acrocentrics XY1Y2 in the male). A group of race specific chromosomes includes 4 metacentric combinations (hn, ip, kq, mo) and two acrocentric chromosomes (g and r). In all 5 shrew specimens examined the same homozygous autosome complement 2Na=20 was found. Data available from neighbouring regions in Russia and, in particular, from adjacent Fennoscandian countries reveal complex relationships between karyotypes of common shrews from the European North. text/html en_US Pensoft Publishers karyotype chromosome race common shrew Sorex araneus Northern Europe European Russia A distinct chromosome race of the common shrew (Sorex araneus Linnaeus, 1758) within the Arctic Circle in European Russia Research Article

10.3897/compcytogen.v4i1.24 2010-07-09 compcytogen

Laboratório de Citogenética, ICB., Belém, Brazil author Gomes, A Laboratório de Genética and Biodiversidade, Santarém, Brazil author Rodrigues, L Laboratório de Citogenética, ICB, Belém, Brazil author Rissino, J Universidade Federal do Pará, Belém-Pará, Brazil author Nagamachi, C Universidade Federal do Pará, Belém – Pará, Brazil author Pieczarka, J 2010-07-09 2010-07-09 2010 Comparative Cytogenetics 1993-078X 1993-0771 4 1 79 85 2010 10.3897/compcytogen.v4i1.24 https://compcytogen.pensoft.net/article/1696/ https://compcytogen.pensoft.net/article/1696/download/pdf/ The genus Rhinophylla Peters, 1865 (Carolliinae: Phyllostomidae) comprises three species: R. pumilio Peters, 1865, R. fischerae Carter, 1966 and R. alethina Handley, 1966. Only the first two species have been cytogenetically studied to date. Previous studies on specimens of Rhinophylla fischerae from two populations from East of Andes (Colombia) showed the karyotype with 2n=34 and FN=56. In this paper, we report the results of cytogenetic analysis of six specimens of Rhinophylla fischerae from Brazil. Probably chromosomal differences can be found among the populations because of the geographic distance. Metaphase chromosomes were obtained in the field by direct extraction of bone marrow. The metaphases were analyzed by conventional staining, G- and C-banding, NOR-staining and FISH with telomeric probes. Rhinophylla fischerae has 2n=38 and Fundamental Number FN=68, with small amounts of constitutive heterochromatin in the centromeric regions of the chromosomes and the long arm of pair 16. Fluorescence in situ hybridization using telomeric probes did not show any interstitial sequences. Hybridization with human 18S and 28S rDNA probes and silver staining revealed the presence of Nucleolar Organizer Regions at the long arms of pairs 16 and 18. The pattern of G-banding showed that this population had a huge chromosome variation compared with previous studies on specimens of Rhinophylla fischerae. The chromosomal differences among populations that have been morphologically classified as R. fischerae suggest that this species should be considered a cryptic species complex, and that the populations from different geographical regions analyzed to date should be considered species of this complex, where the chromosomal rearrangements had key importance. text/html en_US Pensoft Publishers Rhinophylla fischerae Chiroptera karyotype cryptic species Amazon biodiversity Biogeographical karyotypic variation of Rhinophylla fischerae (Chiroptera: Phyllostomidae) suggests the occurrence of cryptic species Research Article

10.3897/compcytogen.v4i1.28 2010-07-09 compcytogen

University of York, York, United Kingdom author Searle, J Unaffiliated, United Kingdom author Fedyk, S Unaffiliated, United Kingdom author Fredga, K Unaffiliated, United Kingdom author Hausser, J Unaffiliated, United Kingdom author Volobouev, V 2010-07-09 2010-07-09 2010 Comparative Cytogenetics 1993-078X 1993-0771 4 1 87 96 2010 10.3897/compcytogen.v4i1.28 https://compcytogen.pensoft.net/article/1697/ https://compcytogen.pensoft.net/article/1697/download/pdf/ A G-band composite karyotype has been prepared for the common shrew (Sorex araneus Linnaeus, 1758). This includes multiple cut-outs of each chromosome arm (in different stages of contraction) derived from chromosome spreads prepared by a variety of methods by the different authors. The important features of each chromosome arm are described. The nomenclature for the chromosome arms follows that of Halkka et al. (1974) as clarified by Fredga, Nawrin (1977) and subsequent authors, i.e. italicised letters of the alphabet are used with a as the largest chromosome arm. Different authors have used a variety of methods to describe the karyotype of (a) individuals and (b) the pattern of variation within populations. Also, definitions of chromosomal ‘race’ differ. We suggest a standardised scheme for the description of individuals, populations and chromosomal races text/html en_US Pensoft Publishers G-bands standard karyotype chromosome race common shrew Sorex araneus Nomenclature for the chromosomes of the common shrew (Sorex araneus) Research Article

10.3897/compcytogen.v4i2.41 2010-12-30 compcytogen

Russian Academy of Sciences, St. Petersburg, author Anokhin, B Christian Albrechts University, Kiel, Germany author Hemmrich-Stanisak, G Christian Albrechts University, Kiel, Germany author Bosch, T 2010-12-30 2010-12-30 2010 Comparative Cytogenetics 1993-078X 1993-0771 4 97 110 2010 10.3897/compcytogen.v4i2.41 https://compcytogen.pensoft.net/article/1698/ https://compcytogen.pensoft.net/article/1698/download/pdf/ The fresh water polyp Hydra L., 1758 (Cnidaria, Hydrozoa) plays a key role as a model organism in modern evolutionary and developmental biology. A complete genome sequence has been published recently for Hydra magnipapillata Ito, 1947 and molecular data are rapidly accumulating in the literature, but little information is available on its chromosomes. In this study, an efficient fluorescence in situ hybridization (FISH) method is described for H. magnipapillata which not only allows identification of the chromosomes but also visualization of the location of individual genetic loci. Together with cDNA and genomic sequencing this may provide the foundation for increasingly precise genetic and physical mapping in this basal metazoan model organism. text/html en_US Pensoft Publishers Hydra magnipapillata karyotype chromosomes fluorescence in situ hybridization (FISH) single-gene detection Qdots Karyotyping and single-gene detection using fluorescence in situ hybridization on chromosomes of Hydra magnipapillata (Cnidaria: Hydrozoa) Research Article

10.3897/compcytogen.v4i2.32 2010-12-30 compcytogen

Universidade de Tras-os-Montes e Alto Douro, Vila Real, Portugal author Bouilly, Karine University of Trás-os-Montes and Alto Douro, Vila Real, Portugal author Chaves, R Universidade de Tras-os-Montes e Alto Douro, Vila Real, Portugal author Fernandes, M Universidade de Tras-os-Montes e Alto Douro, Vila Real, Portugal author Guedes-Pinto, H 2010-12-30 2010-12-30 2010 Comparative Cytogenetics 1993-078X 1993-0771 4 111 121 2010 10.3897/compcytogen.v4i2.32 https://compcytogen.pensoft.net/article/1699/ https://compcytogen.pensoft.net/article/1699/download/pdf/ The Pacific oyster, Crassostrea gigas Thunberg, 1793 (2n = 20) is an economically important mollusc species cultured throughout the world. The most frequently used technique for molecular cytogenetic studies is fluorescence in situ hybridisation which offers new opportunities for the identification of oyster chromosomes. In oysters, it has been used to locate telomeric sequences, satellite DNA, simple sequence repeats, ribosomal RNA genes, and bacteriophage P1 clones. However, regarding chromosome identification, no study has been done with histone H3 gene. Histone H3 is among the most conserved eukaryotic proteins. Most histone H3 genes are repeatedly organised into clusters, which make them an ideal chromosomal marker. In bivalves, some data exist concerning sequence information but little knowledge is available concerning the physical mapping of histone genes. The histone H3 gene was sequenced in C. gigas and phylogenetic analysis revealed that C. gigas was more closely related to Ostrea edulis Linnaeus, 1758 and species of the genus Mytilus Linnaeus, 1758. In C. gigas, the histone H3 gene was mapped on two different pairs of chromosomes, one at an interstitial site on the long arm of chromosome pair 4, and the other on the telomeres of the smaller chromosome pair (pair 10). Polymorphism was detected on the telomeres of pair 10, once it was possible to observe single or double signals. Comparative chromosomal mapping should improve our understanding of bivalve genome organisation. text/html en_US Pensoft Publishers ivalves Crassostrea gigas fluorescence in situ hybridisation histone H3 gene phylogenetics physical mapping Histone H3 gene in the Pacific oyster, Crassostrea gigas Thunberg, 1793: molecular and cytogenetic characterisations Research Article

10.3897/compcytogen.v4i2.46 2010-12-30 compcytogen

Royal holloway, University of London, United Kingdom author Angus, Robert https://orcid.org/https://orcid.org/0000-0002-3860-5617 2010-12-30 2010-12-30 2010 Comparative Cytogenetics 1993-078X 1993-0771 4 123 131 2010 10.3897/compcytogen.v4i2.46 https://compcytogen.pensoft.net/article/1700/ https://compcytogen.pensoft.net/article/1700/download/pdf/ A new genus, Boreonectes gen. n., is erected for the seven S. griseostriatus-group  species  as  well  as eight other American ones listed by Nilsson and Angus (1992) as comprising the Stictotarsus griseostriatus-group as DNA work by Ribera (2003) shows this arrangement to be untenable, and no other genus-group name has been found to be available. Chromosomal investigations have shown that B. griseostriatus (De Geer, 1774) occurs as far west as the Little St Bernard Pass in the Alps, with B. alpestris (Dutton et Angus, 2007) in the Italian Gran Paradiso National Park to the south of this. B. multilineatus (Falkenström, 1922) is recorded from the Pyrenees for the first time, and B. ibericus (Dutton et Angus, 2007) is shown to be present in the Moyen Atlas of Morocco. text/html en_US Pensoft Publishers Dytiscidae Boreonectes new genus S. griseostriatus group sibling species chromosomes distributions Alps Pyrenees Morocco Boreonectes gen. n., a new genus for the Stictotarsus griseostriatus (De Geer) group of sibling species (Coleoptera: Dytiscidae), with additional karyosystematic data on the group Research Article

10.3897/compcytogen.v4i2.47 2010-12-30 compcytogen

Punjabi University, Patiala, India author Kaur, Harbhajan Punjabi University, Punjab, India author Semahagn, B 2010-12-30 2010-12-30 2010 Comparative Cytogenetics 1993-078X 1993-0771 4 133 139 2010 10.3897/compcytogen.v4i2.47 https://compcytogen.pensoft.net/article/1701/ https://compcytogen.pensoft.net/article/1701/download/pdf/ The study of chromosome behaviour has been carried out on males of three species of the Asopinae (Pentatomidae) bugs Andrallus spinidens (Fabricius, 1787), Canthecona furcellata Wolff, 1801 and Perillus bioculatus (Fabricius, 1775) collected in India. All the species have XY sex chromosome system and 12 autosomes (2n=14=12A+XY). The general course of meiosis is fairly uniform and is typical for heteropteran species. However, the species differ in the extent of decondensation of the autosomes during the diffuse stage and the number of ring bivalents at diplotene/diakinesis. Also, a metaphase I arrangement of chromosomes which is different from the typical Pentatomidae type has been observed in C. furcellata and P. bioculatus. text/html en_US Pensoft Publishers chromosomes diffuse stage metaphase arrangement Asopinae Pentatomidae Meiotic behaviour of chromosomes in three predator species of the subfamily Asopinae (Heteroptera: Pentatomidae) Research Article

10.3897/compcytogen.v4i2.50 2010-12-30 compcytogen

Universidade Estadual Paulista (UNESP/IBILCE), São José do Rio Preto, Brazil author Bardella, Vanessa https://orcid.org/0000-0001-5285-986X Universidade Estadual de Londrina (UEL), Londrina, Brazil author Gaeta, M Universidade Estadual de Londrina (UEL), Londrina, Brazil author Vanzela, André Luís Laforga Universidade Estadual Paulista (UNESP/IBILCE), São José do Rio Preto, Brazil author Azeredo-Oliveira, M 2010-12-30 2010-12-30 2010 Comparative Cytogenetics 1993-078X 1993-0771 4 141 149 2010 10.3897/compcytogen.v4i2.50 https://compcytogen.pensoft.net/article/1702/ https://compcytogen.pensoft.net/article/1702/download/pdf/ The Triatominae are distributed in Brazilian regions and are the vector of Chagas disease. This group is also characterized by lower karyotype variability, occurrence of holokinetic chromosomes and inverted meiosis of the sex chromosomes. In this study, we determined the karyotype of four species of the genus Triatoma Laporte, 1832 using chromosome measurement, chromosome banding and FISH with a 45S rDNA probe. All samples showed 2n = 20A+XY with chromosomes sorted by size in decreasing order. Heterochromatin of Triatoma infestans melanosoma Lent, Jurberg, Galvão, Carcavallo, 1994 is distributed among eight autosomes and the sex chromosomes. In Triatoma brasiliensis Neiva, 1911, T. matogrossensis Leite, Barbosa, 1953 and T. rubrovaria Blanchard, 1834, heterochromatin was restricted to the Y chromosome, which was characterized as DAPI+ (weak signal). FISH using a 45S rDNA probe of Drosophila melanogaster Meigen, 1830 showed differences in the number and location of hybridization sites. T. brasiliensis and T. rubrovaria showed the signal on one autosome pair. T. matogrossensis showed signals on both sex chromosomes, and T. infestans melanosoma only on the X chromosome. Conventional banding analysis suggests a closer relationship between T. brasiliensis, T. matogrossensis and T. rubrovaria, except with regard to 45S rDNA location in T. matogrossensis, and a more differentiated karyotype in T. infestans melanosoma. text/html en_US Pensoft Publishers FISH holokinetic chromosomes repetitive DNA Triatoma 45S rDNA Chromosomal location of heterochromatin and 45S rDNA sites in four South American triatomines (Heteroptera: Reduviidae) Research Article

10.3897/compcytogen.v4i2.36 2010-12-30 compcytogen

Bulgarian Academy of Sciences, Sofia, Bulgaria author Grozeva, Snejana https://orcid.org/0000-0003-3196-8068 Zoological Institute, St. Petersburg, St. Petersburg, author Kuznetsova, Valentina G. https://orcid.org/0000-0001-8386-5453 Russian Academy of Sciences, St. Petersburg, author Anokhin, B 2010-12-30 2010-12-30 2010 Comparative Cytogenetics 1993-078X 1993-0771 4 151 160 2010 10.3897/compcytogen.v4i2.36 https://compcytogen.pensoft.net/article/1703/ https://compcytogen.pensoft.net/article/1703/download/pdf/ Bugs (Insecta: Heteroptera) are frequently used as examples of unusual cytogenetic characters, and the family Cimicidae is one of most interest in this respect. We have performed a cytogenetic study of the common bed bug Cimex lectularius Linnaeus, 1758 using both classical (Schiff-Giemsa and AgNO3-staining) and molecular cytogenetic techniques (base-specific DAPI/CMA3 fluorochromes and FISH with an 18S rDNA probe). Males originated from a wild population of C. lectularius were found to have 2n = 26 + X1X2Y, holokinetic chromosomes, 18S rRNA genes located on the X1 and Y chromosomes; achiasmate male meiosis of a collochore type; MI and MII plates nonradial and radial respectively. text/html en_US Pensoft Publishers holokinetic chromosomes karyotype multiple sex chromosomes achiasmate collochore meiosis FISH with an 18S rDNA probe Cimex lectularius Bed bug cytogenetics: karyotype, sex chromosome system, FISH mapping of 18S rDNA, and male meiosis in Cimex lectularius Linnaeus, 1758 (Heteroptera: Cimicidae) Research Article

10.3897/compcytogen.v4i2.45 2010-12-30 compcytogen

N.I. Vavilov Institute of General Genetics, Moscow, author Shaikevich, E N.I. Vavilov Institute of General Genetics, Moscow, author Zakharov, I 2010-12-30 2010-12-30 2010 Comparative Cytogenetics 1993-078X 1993-0771 4 161 174 2010 10.3897/compcytogen.v4i2.45 https://compcytogen.pensoft.net/article/1704/ https://compcytogen.pensoft.net/article/1704/download/pdf/ Polymorphism of the mtDNA gene COI encoding cytochrome C oxidase subunit I was studied in the mosquitoes Culex pipiens Linnaeus, 1758 and C. torrentium Martini, 1925 from sixteen locations in Russia and in three laboratory strains of subtropical subspecies of the C. pipiens complex. Representatives of this complex are characterized by a high ecological plasticity and there are significant ecophysiological differences between its morphologically similar members. The full-size DNA sequence of the gene COI spans 1548 bp and has a total A+T content of 70.2 %. The TAA is a terminating codon in all studied representatives of the C. pipiens complex and C. torrentium. 64 variable nucleotide sites (4 %) were found, fifteen haplotypes were detected, and two heteroplasmic specimens of C. torrentium were recorded. COI haplotype diversity was low  in Wolbachia–infected populations of the C. pipiens complex. Monomorphic haplotypes were found in C. p. quinquefasciatus and C. p. pipiens f. molestus. Three haplotypes were detected for the C. p. pipiens, but these haplotypes were not population-specific. On the other hand, each of the ten studied Wolbachia-uninfected C. torrentium individuals from three different populations had unique mitochondrial haplotypes. Polymorphism of the 478-bp ITS2 nucleotide sequences was similar in infected C. p. pipiens and C. p. pipiens f. molestus and uninfected C. torrentium specimens. The ITS2 genetic distance between C. p. pipiens and C. torrentium reached 12.5 %. Possible effects of Wolbachia invasion on C. pipiens populations are discussed. text/html en_US Pensoft Publishers Polymorphism Culex torrentium Culex pipiens mitochondrial COI Polymorphism of mitochondrial COI and nuclear ribosomal ITS2 in the Culex pipiens complex and in Culex torrentium (Diptera: Culicidae) Research Article

10.3897/compcytogen.v4i2.48 2010-12-30 compcytogen

Universidade Estadual de Maringá, Parana, Brazil author Rosa, Renata https://orcid.org/0000-0003-4258-7244 Universidade Federal de São Carlos, São Carlos, Brazil author Rubert, Marceléia https://orcid.org/0000-0002-2832-5929 Universidade Estadual de Londrina, Londrina, Brazil author Giuliano-Caetano, L. Universidade Estadual de Maringá, Maringá, Brazil author Martins-Santos, I 2010-12-30 2010-12-30 2010 Comparative Cytogenetics 1993-078X 1993-0771 4 175 184 2010 10.3897/compcytogen.v4i2.48 https://compcytogen.pensoft.net/article/1705/ https://compcytogen.pensoft.net/article/1705/download/pdf/ Cytogenetic analyses were performed on Hoplias malabaricus (Bloch, 1794) from the Taquari River, Parana River drainage, revealing two sympatric karyomorphs. One karyomorph was characterized by 2n = 40 m/sm and 2n = 39 m/sm chromosomes for females and males, respectively, and an X1X1X2X2/X1X2Y sex chromosome system. In the second karyomorph, specimens showed 2n = 42 m/sm chromosomes, without sex-related heteromorphism. Both karyomorphs were characterized by a distribution of heterochromatin in the pericentromeric and telomeric regions. In addition to the differences in the diploid numbers and the sex chromosomes, the GC-rich sites and the nucleolar organizer regions also showed clear differences between the karyomorphs. Coupled with the occurrence of unique chromosomal features within each karyomorph, the fact that hybrids have not been identified in the sampled population provides additional support of the existence of a species complex in H. malabaricus. text/html en_US Pensoft Publishers chromosomal banding karyotype differentiation species complex Differential chromosomal markers between sympatric karyomorphs of the fish Hoplias malabaricus (Bloch, 1794) (Characiformes: Erythrinidae) Research Article

10.3897/compcytogen.v4i2.49 2010-12-30 compcytogen

Universidade Estadual Paulista (UNESP), Botucatu, Brazil author Mariguela, T Universidad de Oriente, Porlamar, Venezuela author Nirchio, M Universidad de Oriente, Porlamar, Venezuela author Ron, E Universidad de Oriente, Porlamar, Venezuela author Gaviria, J Universidade Estadual Paulista (UNESP), Botucatu, Brazil author Foresti, Fausto https://orcid.org/0000-0002-0862-0445 Universidade Estadual Paulista, Botucatu, Brazil author Oliveira, Claudio 2010-12-30 2010-12-30 2010 Comparative Cytogenetics 1993-078X 1993-0771 4 185 193 2010 10.3897/compcytogen.v4i2.49 https://compcytogen.pensoft.net/article/1706/ https://compcytogen.pensoft.net/article/1706/download/pdf/ A cytogenetic analysis by conventional Giemsa staining, silver staining, C-banding, and fluorescence in situ hybridization (FISH) was carried out on Brycon amazonicus from Caicara del Orinoco, Venezuela. The karyotype of this species is characterized by the presence of 2n = 50 chromosomes, a karyotypic formula 22m+14sm+14st, and a fundamental number of 100 chromosomal arms. Nucleolar organizer regions (NORs) and 18S rDNA genes are located in the terminal regions of the long arms of the second pair of subtelocentric chromosomes, corresponding to pair 13. C-banding revealed heterochromatin distribution in only seven chromosome pairs. The largest metacentric pair (number 1) possesses a paracentromeric block equilocally distributed in both chromosome arms, whereas in pairs 12 to 17 positive C band blocks were located in the paracentromeric region of the long arm, close to the centromere. Analysis performed with 5S rDNA gene revealed a terminal site located on the short arm of one small submetacentric chromosome (pair 15) corroborating previous studies with this repetitive gene showing an apparent conservation of 5S rDNA in the genome of these fish species. The data obtained in this study reinforce the chromosomal conservativeness in the species of the genus Brycon, related not only to the macro-chromosomal structure (diploid number, karyotypic formula, and fundamental number), but also to the repeated DNAs, such as heterochromatic regions and ribosomal DNAs. These data will contribute to a better understanding of chromosomal evolution in both Brycon and Characidae fishes. text/html en_US Pensoft Publishers Characidae C-banding karyotype NORs 18S rDNA 5S rDNA Cytogenetic characterization of Brycon amazonicus (Spix et Agassiz, 1829) (Teleostei: Characidae) from Caicara del Orinoco, Venezuela Research Article

10.3897/compcytogen.v4i2.43 2010-12-30 compcytogen

Russian Academy of Sciences, Moscow, author Borisov, Yu Russian Academy of Sciences, Moscow, author Cherepanova, E Russian Academy of Sciences, Moscow, author Orlov, Victor 2010-12-30 2010-12-30 2010 Comparative Cytogenetics 1993-078X 1993-0771 4 195 201 2010 10.3897/compcytogen.v4i2.43 https://compcytogen.pensoft.net/article/1707/ https://compcytogen.pensoft.net/article/1707/download/pdf/ Karyological study of 75 specimens of the common shrew, Sorex araneus Linnaeus, 1758, from 8 localities in the Berezina River basin (eastern Belarus) was carried out. A wide hybrid zone (not less than 100 km) between the northern West Dvina chromosome race (XX / XYY, af, bc, gm, hk, ip, jl, no, qr, tu) and the southern Turov race (XX / XYY, af, bc, g, h/k, i, jl, m, n, o, p, q, r, tu) was revealed in this region. Frequencies of fused-unfused arms comprising four diagnostic metacentrics of the West Dvina race (g/m, h/k, n/o, q/r) were calculated in all capture sites. Taking into consideration the absence of metacentric ip in specimens from six northern localities, the Borisov (Bs) race (XX / XYY, af, bc, g/m, h/k, i, jl, n/o, p, q/r, tu) (Orlov, Borisov, 2009) was distinguished in these sites. Common shrews from two southern localities on the right and left banks of the Berezina River (Berezino vicinity) were referred to the Turov race. The presence of four metacentrics descended from the West Dvina race in the Bs race testifies to the hypothesis expressed earlier that the polymorphic populations of the S. araneus between the Dnieper and Berezina Rivers originated as a result of the West Dvina race spreading from the north and of hybridization between this race and local populations with acrocentric chromosomes. text/html en_US Pensoft Publishers common shrew Sorex araneus chromosome race hybrid zone A wide hybrid zone of chromosome races of the common shrew, Sorex araneus Linnaeus, 1758 (Mammalia), between the Dnieper and Berezina Rivers (Belarus) Research Article

10.3897/compcytogen.v4i2.44 2010-12-30 compcytogen

Zoological Institute, Russian Academy of Sciences, St. Petersburg, author Gavrilov-Zimin, Ilya https://orcid.org/0000-0003-1993-5984 Russian Academy of Sciences, St. Petersburg, author Trapeznikova, I 2010-12-30 2010-12-30 2010 Comparative Cytogenetics 1993-078X 1993-0771 4 203 205 2010 10.3897/compcytogen.v4i2.44 https://compcytogen.pensoft.net/article/1708/ https://compcytogen.pensoft.net/article/1708/download/pdf/ Phenacoccus peruvianus Granada de Willink, 2007, Ph. prope avenae Borchsenius, 1949, Ph. hordei (Lindeman, 1886), Вalanococcus boratynskii Williams, 1962, Trionymus  radicum (Newstead, 1895), Rhizoecus halophilus (Hardy, 1868) were studied karyologically for the first time. All species demonstrate 2n=10 and a Lecanoid genetic system. Photos of karyotypes of all studied species are given. text/html en_US Pensoft Publishers mealybugs scale insects karyotype chromosome number Karyotypes of six previously unstudied European mealybugs (Homoptera: Pseudococcidae) Research Article

10.3897/compcytogen.v4i2.42 2010-12-30 compcytogen

Institute of Experimental Biology, Moscow, and the Zootechnical Institute in Balashikha, author Dubinin, N Institute of Experimental Biology, Moscow, and the Zootechnical Institute in Balashikha, author Sokolov, N Institute of Experimental Biology, Moscow, and the Zootechnical Institute in Balashikha, author Tiniakov, G 2010-12-30 2010-12-30 2010 Comparative Cytogenetics 1993-078X 1993-0771 4 207 214 2010 10.3897/compcytogen.v4i2.42 https://compcytogen.pensoft.net/article/1709/ https://compcytogen.pensoft.net/article/1709/download/pdf/ (Editorial preface). The publication is presented in order to remind us of one of dramatic pages of the history of genetics. It re-opens for the contemporary reader a comprehensive work marking the priority change from plant cytogenetics to animal cytogenetics led by wide population studies which were conducted on Drosophila polytene chromosomes. The year of the publication (1937) became the point of irretrievable branching between the directions of Old World and New World genetics connected with the problems of chromosome variability and its significance for the evolution of the species. The famous book of T. Dobzhansky (1937) was published by Columbia University in the US under the title “Genetics and the origin of species”, and in the shadow of this American ‘skybuilding’ all other works grew dim. It is remarkable that both Dobzhansky and Dubinin come to similar conclusions about the role of chromosomes in speciation. This is not surprising given that they both might be considered as representatives of the Russian genetic school, by their birth and education. Interestingly, Dobzhansky had never referred to the full paper of Dubinin et al. (1937), though a previous short communication in Nature (1936) was included together with all former papers on the related subject. In full, the volume of the original publication printed in the Biological Journal in Moscow comprised 47 pages, in that number 41 pages of the Russian text accompanied by 16 Figs, a table and reference list, and, above all, 6 pages of the English summary. This final part in English is now reproduced in the authors’ version with the only addition being the reference list in the originally printed form. text/html en_US Pensoft Publishers Intraspecific chromosome variabilit Intraspecific chromosome variability Research Article

10.3897/compcytogen.v5i1.1143 2011-05-05 compcytogen

Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina author Poggio, María Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina author Bressa, María José https://orcid.org/0000-0002-0191-6518 Laboratorio de Citogenética y Evolución, Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buen, Argentina author Papeschi, Alba 2011-05-05 2011-05-05 2011 Comparative Cytogenetics 1993-078X 1993-0771 5 1 1 22 2011 DP Ambrose author 1999 Assassin bugs. 1999 Molecular evidence for genetic exchange among ribosomal genes on non-homologous chromosomes in man and apes. N Arnheim author 1980 text Proceedings of National Academy of Sciences USA 1980 77 7323 7527 Cytogenetic analysis in the spermatogenesis of Triatoma melanosoma (Reduviidae; Heteroptera). 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HD Slack author 1939 text Nature 1939 Meiotic sister chromatid cohesion in holocentric sex chromosomes of three heteropteran species is maintained in absence of axial elements. JA Suja author 2000 text Chromosoma 2000 109 35 43 Chromosome evolution in the Heteroptera (Hemiptera): agmatoploidy versus aneuploidy. DBJ Thomas author 1987 text Annals of the Entomological Society of America 1987 80 6 720 730 Synapsis with and without recombination in the male meiosis of the leaf-footed bug Holhymenia rubiginosa (Coreidae, Heteroptera). MA Toscani author 2008 text Genetica 2008 132 173 178 Pachytene mapping in the female silkworm Bombyx mori L. (Lepidoptera). W Traut author 1976 text Chromosoma 1976 58 275 284 The evolution of sex chromosomes in insects: differentiation of sex chromosomes in flies and moths. W Traut author 1999 text European Journal of Entomology 1999 96 227 235 Hemiptera II: Heteroptera. N Ueshima author B John author 1979 text Animal Cytogenetics 1979 Inverted meiosis: the true bugs as a model to study. A Viera author R Benavente author 2009 text Meiosis 2009 137 156 Cladistic analysis of Reduviidae (Heteroptera: Cimicomorpha) based on morphological characters. C Weirauch author 2008 text Systematic Entomology 2008 33 2 229 274 Molecular phylogeny of the assasin bugs (Hemiptera: Reduviidae), based on mitocondrial and nuclear ribosomal genes. C Weirauch author 2009 text Molecular Phylogenetics and Evolution 2009 53 287 299 MJD White author 1973 Animal cytology and evolution. 1973 Phylogeny of the holometabolous insect orders: molecular evidence. MF Whiting author 2002 text Zoologica Scripta 2002 31 1 3 15 Studies on chromosomes. I. The behavior of the idiochromosomes in Hemiptera. EB Wilson author 1905a text Journal of Experimental Zoology 1905a 2 371 405 Studies on chromosomes. II. The paired microchromosomes, idiochromosomes and heterotropic chromosomes in Hemiptera. EB Wilson author 1905b text Journal of Experimental Zoology 1905b 2 507 545 Studies on chromosomes. IV The “accessory” chromosome in Syromastes and Pyrrhocoris with a comparative review of the types of sexual differences of the chromosome groups. EB Wilson author 1909a text Journal of Experimental Zoology 1909a 6 1 69 99 Studies on chromosomes. V. The chromosomes of Metapodius. A contribution to the hypothesis of the genetic continuity of chromosomes. EB Wilson author 1909b text Journal of Experimental Zoology 1909b 6 147 205 Studies on chromosomes. VII. A review of the chromosomes of Nezara; with some more general considerations. EB Wilson author 1911 text Journal of Morphology 1911 22 1 71 110 Physical mapping of ribosomal RNA genes in peonies (Paeonia, Paeoniaceae) by fluorescent in situ hybridization: implications for phylogeny and concerted evolution. D Zhang author 1999 text American Journal of Botany 1999 86 735 740 10.3897/compcytogen.v5i1.1143 https://compcytogen.pensoft.net/article/1710/ https://compcytogen.pensoft.net/article/1710/download/pdf/ In the present work, we analysed the male meiosis, the content and distribution of heterochromatin and the number and location of nucleolus organizing regions in Microtomus lunifer (Berg, 1900) by means of standard technique, C- and fluorescent bandings, and fluorescent in situ hybridization with an 18S rDNA probe. This species is the second one cytogenetically analysed within the Hammacerinae. Its male diploid chromosome number is 31 (2n=28+X1X2Y), including a minute pair of m-chromosomes. The diploid autosomal number and the presence of m-chromosomes are similar to those reported in M. conspicillaris (Drury, 1782) (2n=28+XY). However, M. lunifer has a multiple sex chromosome system X1X2Y (male) that could have originated by fragmentation of the ancestral X chromosome. Taking into account that M. conspicillaris and M. lunifer are the only two species within Reduviidae that possess m-chromosomes, the presence of this pair could be a synapomorphy for the species of this genus. C- and fluorescent bandings showed that the amount of heterochromatin in M. lunifer was small, and only a small CMA3 bright band was observed in the largest autosomal pair at one terminal region. FISH with the 18S rDNA probe demonstrated that ribosomal genes were terminally placed on the largest autosomal pair. Our present results led us to propose that the location of rDNA genes could be associated with variants  of the sex chromosome systems in relation with a kind of the sex chromosome systems within this family. Furthermore, the terminal location of NOR in the largest autosomal pair allowed us to use it as a chromosome marker and, thus, to infer that the kinetic activity of both ends is not a random process, and there is an inversion of this activity. text/html en_US Pensoft Publishers Hemiptera Reduviidae Hammacerinae meiosis m-chromosomes evolutionary trends rDNA-FISH Male meiosis, heterochromatin characterization and chromosomal location of rDNA in Microtomus lunifer (Berg, 1900) (Hemiptera: Reduviidae: Hammacerinae) Research Article

10.3897/compcytogen.v5i1.975 2011-05-05 compcytogen

Uni Freiburg, Merzhausen, Germany author Wülker, Wolfgang F. Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia author Kiknadze, Iya Siberian Branch of Russian Academy of Sciences, Novosibirsk, author Istomina, A 2011-05-05 2011-05-05 2011 Comparative Cytogenetics 1993-078X 1993-0771 5 1 23 46 2011 Contribution à l’étude des premiers états des chironomides du Tchad (Insectes, Diptéres) (1e note). C Dejoux author 1968 text Hydrobiologia 1968 31 449 464 Contribution à l’étude des premiers états des chironomides du Tchad (Insectes, Diptéres) (4e note) C Dejoux author 1970 text Cahiers ORSTOM, série Hydrologie 1970 4 2 39 51 Standardization of chromosome arms B, C, and D in Chironomus (Diptera: Chironomidae). Gy Dévai author 1989 text Acta biologica debrecina, supplement oecologica Hungarica 1989 2 1 79 92 Th Dobzhansky author 1970 Genetics of the evolutionary process 1970 A study of Chironomidae (Diptera) of Africa south of the Sahara, part III. P Freeman author 1957 text Bulletin of the British Museum (Natural History) Entomology 1957 5 9 321 426 Chromosomenevolution bei Chironomus. II. Chromosomenumbauten und phylogenetische Beziehungen der Arten. H-G Keyl author 1962 text Chromosoma 1962 13 464 514 J Kieffer author 1908 Chironomidae 1908 13 155 162 Chironomidae et Cecidymyidae Voyage de Ch. alluaudi. Et R. Jeanell en Afrique Orientale (1911–1912) J Kieffer author 1913 text Résultats scientifique Insectes Diptéres 1913 1 43 Chironomidae de l’Afrique de sud. J Kieffer author 1923 text Annales de la Société scientifique de Bruxelles 1923 42 1 382 388 Similarity analysis of inversion banding sequences in chromosomes of Chironomus species (breakpoint phylogeny) II Kiknadze author N Kolchanov author 2003 text Bioinformatics of Genome Regulation and Structure 2003 245 253 Pattern of chromosomal polymorphism during population and species divergence in Chironomus (Diptera, Chironomidae) II Kiknadze author 2004 text Siberian Oekological Journal 2004 11 5 635 652 Chromosomes and continents II Kiknadze author N Dobretsov author 2008 text Biosphere Origin and Evolution 2008 349 369 M King author 1993 Species Evolution: the Role of Chromosomal Change 1993 Chromosomes as tools in taxonomy and phylogeny of Chironomidae (Diptera) J Martin author 1979 text Entomologica Scandinavica 1979 67 74 J Martin author 2010 Australian Chironomus species 2010 Evolutionary cytology in the genus Chironomus Meigen. Studies in Natural Sciences. J Martin author 1974 text (Portales, New Mexico) 1974 1 12 1 12 Notes on some larval and pupal chironomids (Diptera) from. Lake Kariba, Rhodesia. AJ McLachlan author 1969 text Journal of Natural History 1969 3 261 293 Some immature stages of the subgenus Chironomus (Meigen) (Diptera: Chironomidae) from Malawi, Central Africa. Journal of Entomology. AJ McLachlan author 1971 text Series B, Taxonomy 1971 40 2 173 178 CRW Wiedemann author 1830 Aussereuropäische Zweiflüglige Insekten 1830 2 MJD White author 1977 Animal cytology and evolution 1977 Basic patterns in the chromosome evolution of the genus Chironomus (Diptera). W Wülker author 1980 text Zeitschrift fûr Zoologische Systematik und Evolutionsforschung 1980 18 2 112 123 The role of chromosomes in chironomid systematics, ecology and phylogeny. W Wülker author LC Jr Ferrington author 2010 text Proceedings of the XV International Symposium on Chironomidae 2010 1 13 Computer-assisted studies of chromosome evolution in the genus Chironomus (Dipt.). Comparative and integrated analysis of chromosome arms A, E and F. W Wülker author 1989 text Acta biologica debrecina, supplement oecologica Hungarica 1989 2 1 373 387 South American Chironomus (Dipt.) – karyotypes and their relations to North America. W Wülker author 1989 text Acta biologica debrecina, supplement oecologica Hungarica 1989 2 1 389 397 Comparative genome and proteome analysis of Anopheles gambiae and Drosophila melanogaster. EM Zdobnov author 2002 text Science 2002 298 149 159 10.3897/compcytogen.v5i1.975 https://compcytogen.pensoft.net/article/1711/ https://compcytogen.pensoft.net/article/1711/download/pdf/ The karyotypes of six African Chironomus species (Ch. alluaudi Kieffer, 1913, Ch. transvaalensis Kieffer, 1923, Ch. sp. Nakuru, Ch. formosipennis Kieffer, 1908, Ch. prope pulcher Wiedemann, 1830, Ch. sp. Kisumu) were investigated; four of these karyotypes were described for the first time (Ch. sp. Nakuru, Ch. formosipennis, Ch. prope pulcher, Ch. sp. Kisumu). Of the six Chironomus karyotypes, three had “pseudothummi” cytocomplex chromosome arms combinations AE CD BF G (Ch. alluaudi, Ch. transvaalensis, Ch. sp. Nakuru), two had “thummi” cytocomplex arms combinations AB CD EF G (Ch. formosipennis, Ch. prope pulcher), and one had “parathummi” arm combinations AC BF DE G (Ch. sp. Kisumu). Thus, three of the ten main cytocomplexes known were detected in Africa. Detailed photomaps of all chromosome arms, with the exception of arms B and G, were prepared for the karyotypes of Ch. alluaudi, Ch. transvaalensis, Ch. sp. Nakuru, Ch. prope pulcher; the karyotypes of Ch. formosipennis, Ch. sp. Kisumu could only be fragmentarily mapped. Endemic African banding sequences were characteristic for most of the chromosomal arms in all species studied. However, basic sequences, which can be present in different Chironomus species on different continents (Wülker, 1980; Kiknadze et al., 2008), were also detected also in several African species (Ch. alluaudi, Ch. sp. Nakuru, and Ch. formosipennis). The banding sequences of African species studied allow discussion of the derivation of modern banding patterns from hypothetical species, living before separation of cytocomplexes and continents. text/html en_US Pensoft Publishers Chironomus karyotype banding sequences chromosomal polymorphism chromosomal evolution Karyotypes of Chironomus Meigen (Diptera: Chironomidae) species from Africa Research Article

10.3897/compcytogen.v5i1.962 2011-05-05 compcytogen

Centro de Biologia Ambiental/Departamento de Biologia Animal/, Faculdade de Ciências, Universidade de Lisboa, Lisboa, Portugal author Proença, Sonia J. R. Centro de Biologia Ambiental/Departamento de Biologia Animal/, Faculdade de Ciências, Universidade de Lisboa, Lisboa, Portugal author Serrano, Artur R.M. Universidad de Murcia, Murcia, Spain author Serrano, Jose https://orcid.org/0000-0002-1565-5216 2 Departamento de Zoología y Antropología Física, Campus de Espinardo, Universidad de Murcia, Murcia, Spain author Galian, Jose https://orcid.org/0000-0002-1415-3767 2011-05-05 2011-05-05 2011 Comparative Cytogenetics 1993-078X 1993-0771 5 1 47 59 2011 Karyological analysis of Cylindera trisignata (Latreille, Dejean, 1822) from Portugal (Coleoptera, Cicindelidae). MJ Collares-Pereira author 1990 text Genetica 1990 82 79 83 JA Coyne author 2004 Speciation. 2004 Meiosis in male tiger beetle Cicindela catena Fabr. (Cicindelidae: Coleoptera). J Dasgupta author 1967 text Science Culture 1967 33 491 493 Physical mapping of rDNA genes in the ground beetle Carabus and related genera (Coleoptera: Carabidae). P De la Rúa author 1996 text Journal of Zoological Systematics and Evolutionary Research 1996 34 95 101 Non-allopatric speciation in animals. DJ Futuyma author 1980 text Systematic Zoology 1980 29 254 271 Phylogenetic relationships in West Mediterranean Scaritina inferred from mitochondrial COI sequences and karyotype analysis. J Galián author 1999 text Journal of Zoological Systematics and Evolutionary Research 1999 37 85 92 The origin of multiple sex chromosomes in tiger beetles. J Galián author 2002 text Molecular Biology and Evolution 2002 19 1792 1796 Cytogenetic analysis of Australian tiger beetles (Coleoptera: Cicindelidae): chromosome number, sex-determining system and localisation of rDNA gene. J Galián author 1999 text Journal of Zoological Systematics and Evolutionary Research 1999 37 1 6 Karyotypes of nine species of Cicindelini and cytotaxonomic notes on Cicindelinae (Coleoptera, Carabidae). J Galián author 1990 text Genetica 1990 82 17 24 Localisation and activity of rDNA genes in tiger beetles (Coleoptera: Cicindelidae). J Galián author 1995 text Heredity 1995 74 524 530 Evolutionary dynamics of autosomal-heterosomal rearrangements in a multiple-X chromosome system of tiger beetles (Cicindelidae). J Galián author 2007 text BMC Evolutionary Biology 2007 7 Die Nicht-Homologen-Assoziation multipler Geschlechtschromosomen in der Spermatogenese von Cicindela hybrida (Coleoptera). E Giers author 1977 text Ph.D. Dissertation 1977 Mos as a tool for genome-wide insertional mutagenesis in Caenorhabditis elegans: results of a pilot study. L Granger author 2004 text Nucleic Acids Research 2004 32 Hétérochromosomes de Cicindèles. HA Guénin author 1952 text Revue Suisse de Zoologie 1952 59 277 282 Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: 1-step method. WM Howell author 1980 text Experientia 1980 36 M King author 1993 Species evolution the role of chromosome change. 1993 Chromosomal evolution and speciation: a recombination-based approach. K Livingstone author 2004 text New Phytologist 2004 161 107 112 W Lorenz author 2005 Systematic list of extant ground beetles of the world. (Insecta Coleoptera “Geadephaga”: Trachypachidae and Carabidae incl. Paussinae, Cicindelinae, Rhysodinae). Second Edition. 2005 A quantitative analysis of species descriptions of tiger beetles (Coleoptera: Cicindelidae), from 1758 to 2004, and notes about related development in biodiversity studies. DL Pearson author 2005 text Coleopterists’ Bulletin 2005 59 184 193 Chromosome evolution in the genus Cicindela: Physical mapping and activity of rDNA loci in the tiger beetles species Cicindela littoralis and C. Flexuosa. SJR Proença author 2003 text Journal of Zoological Systematics and Evolutionary Research 2003 41 227 232 First record on the cytotaxonomy of cicindelids (Insecta, Coleoptera) from an Afrotropical region. SJR Proença author 1999 text Caryologia 1999 52 37 47 Cytogenetic variability in genus Odontocheila(Coleoptera, Cicindelidae): karyotypes, C-banding, NORs and localisation of ribosomal genes of O. confusa and O. nodicornis. SJR Proença author 2002a text Genetica 2002a 114 237 245 An unusual karyotype with low chromosome number in Megacephalini, a basal group of tiger beetles (Coleoptera, Cicindelidae): cytogenetic characterisation by C-banding and location of rDNA genes. SJR Proença author 2002b text Hereditas 2002b 137 202 207 Chromosome evolution in tiger beetles: Karyotypes and localization of 18S rDNA loci in Neotropical Megacephalini (Coleoptera, Cicindelidae). SJR Proenca author 2005 text Genetics and Molecular Biology 2005 28 725 733 Chromosomal rearrangements and speciation. LH Rieseberg author 2001 text Trends in Ecology and Evolution 2001 16 351 358 Variability in rDNA loci in Iberian species of the genus Zabrus (Coleoptera: Carabidae) detected by fluorescence in situ hybridization. JF Sánchez-Gea author 2000 text Genome 2000 43 22 28 Cytotaxonomic study of Cephalota hispanica (Gory, 1833) and Spiralia maura (Linnaeus, 1758), two cicindelids from Portugal (Coleoptera). ARM Serrano author 1989 text Genetica 1989 79 69 75 Further analysis of the cytotaxonomy of tiger beetles (Coleoptera: Cicindelidae) from South Portugal. ARM Serrano author 1992 text Nucleus 1992 35 19 24 A review of karyotypic evolution and phylogeny of carabid beetles (Coleoptera, Carabidae). J Serrano author GE Ball author 1998 text Phylogeny and Classification of Caraboidea. 1998 191 228 Cicindelid beetles without multiple sex chromosomes (Coleoptera, Caraboidea). J Serrano author 1986 text Canadian Journal of Genetics and Cytology 1986 28 235 239 The role of chromosomal change in speciation. F Spirito author DJ Howard author 1998 text Endless forms: species and speciation. 1998 320 329 MJD White author 1978 Modes of Speciation. 1978 J Wiesner author 1992 Checklist of the Tiger Beetles of the World. 1992 Cytogenetic analysis of Ctenostomini by C-banding and rDNA localization and its relevance to the knowledge of the evolution of tiger beetles (Coleoptera: Cicindelidae). AA Zacaro author 2004 text Genetica 2004 122 261 268 10.3897/compcytogen.v5i1.962 https://compcytogen.pensoft.net/article/1712/ https://compcytogen.pensoft.net/article/1712/download/pdf/ The ribosomal clusters of six Paleartic taxa belonging to the tiger beetle genera Cephalota Dokhtourow, 1883 and Cylindera Westwood, 1831, with multiple sex chromosomes (XXY, XXXY and XXXXY) have been localised on mitotic and meiotic cells by fluorescence in situ hybridization (FISH), using a PCR-amplified 18S rDNA fragment as a probe. Four patterns of rDNA localization in these tiger beetles were found: 1. Two clusters located in one autosomal pair; 2. Two clusters located in one autosomal pair and one in an X chromosome; 3. Three clusters located in three heterosomes (XXY); 4. Two clusters located in one autosomal pair and two in the heterosomes (one of the Xs and the Y). These results illustrate that ribosomal cistrons have changed their number and localization during the evolution of these genera, showing a dynamic rather than a conservative pattern. These changes in rDNA localization are uncoupled with changes in the number of autosomes and/or heterosomes. A mechanism that involves transposable elements that carry ribosomal cistrons appears to be the most plausible explanation for these dynamics that involve jumping from one location in the genome to another, in some cases leaving copies in the original location. text/html en_US Pensoft Publishers Cephalota Cylindera Cicindelini Coleoptera FISH ribosomal genes chromosome evolution Patterns of rDNA chromosomal localization in Palearctic Cephalota and Cylindera (Coleoptera: Carabidae: Cicindelini) with different numbers of X-chromosomes Research Article

10.3897/compcytogen.v5i1.1141 2011-05-05 compcytogen

Universidade Federal do Rio Grande do Norte, Brazil author Lima, Lorena Universidade Federal do Rio Grande do Norte, Brazil author Martinez, Pablo Universidade Federal do Rio Grande do Norte, Natal - RN, Brazil author Molina, Wagner https://orcid.org/0000-0002-6695-0952 2011-05-05 2011-05-05 2011 Comparative Cytogenetics 1993-078X 1993-0771 5 1 61 69 2011 Cytogenetic analysis of coral reefs fishes from Brazil (Families Pomacanthidae and Chaetodontidae). PRAM Affonso author 2001 text Cytologia 2001 66 379 384 Karyotypic diversification in two Atlantic species of Holocentridae (Pisces, Beryciformes). TOF Bacurau author 2004 text Caryologia 2004 57 300 304 Patterns of genome size evolution in tetraodontiform fishes. EL Brainerd author 2001 text Evolution 2001 55 2363 2368 Characterization of the pufferfish (Fugu) genome as a compact model vertebrate genome. S Brenner author 1993 text Nature 1993 336 265 268 Cytogenetics of nine species of Mediterranean blennies and additional evidence for an unusual multiple sex-chromosome system in Parablennius tentacularis (Perciformes, Blenniidae). V Caputo author 2001 text Chromosome Res. 2001 9 3 12 Characterization and repeat analysis of the compact genome of the freshwater pufferfish Tetraodon nigroviridis. HR Crollius author 2000 text Genome Res. 2000 10 939 949 The mitochondrial genome of the pufferfish, Fugu rubripes, and ordinal teleostean relationships. C Elmerot author 2002 text Gene 2002 295 163 172 Ecology and zoogeography of deep reef fishes in northeastern Brazil. BM Feitoza author 2005 text B. Mar. Sci. 2005 76 725 742 C Fischer author 2000 2000 KA Frickhinger author 1995 1995 JR Gold author 1990 1990 F Grützner author 1999 1999 Mapping of the 18S and 5S ribosomal genes in the fish Prochilodus argenteus Agassiz, 1829 (Characiformes, Prochilodontidae). 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M Nirchio author 2009 text Genetica 2009 135 1 1 5 E Pauls author 1993 1993 AM Pendás author 1993 1993 Cytogenetic analysis using quantitative, high sensitivity, fluorescence hybridization. D Pinkel author 1986 text Proc. Natl. Acad. Sci. USA. 1986 83 2934 2938 LA Rocha author 2003 2003 Sex chromosomes in Stephanolepis hispidus (Monacanthidae, Tetraodontiformes). LG Sá-Gabriel author 2004 text Cytologia 2004 69 447 452 Karyotype diversification in fishes of the Balistidae, Diodontidae and Tetraodontidae (Tetraodontiformes). LG Sá-Gabriel author 2005 text Caryologia 2005 58 229 237 F Santini author 2003 2003 D Schweizer author 1980 1980 At Sumner author 1972 1972 MR Vicari author 2003 2003 R Winterbottom author 1974 1974 Y Yamanoue author 2008 2008 10.3897/compcytogen.v5i1.1141 https://compcytogen.pensoft.net/article/1713/ https://compcytogen.pensoft.net/article/1713/download/pdf/ The Tetraodontiformes are the most derived group of teleostean fish. Among other apomorphies, they are characterized by a high degree of fusions or significant bone loss in the head and body. In the early phylogenetic proposals presented for this order, the families Balistidae and Monacanthidae have been unanimously considered to be closely related. Although they have moderate species diversity, they are scarcely known in cytogenetic aspect and chromosomal pattern comparisons between these groups have yet to be established. The species Cantherhines macrocerus (Hollard,1853), C. pullus (Ranzani, 1842) (Monacanthidae) and Melichthys niger (Bloch, 1786) (Balistidae) were cytogenetically analyzed using conventional (Ag-impregnation, C-banding, CMA3- and DAPI-fluorescence) and molecular (FISH with an 18S rDNA probe) cytogenetic protocols. The karyotypes of all three species were very similar possessing diploid chromosome numbers 2n = 40 and composed exclusively of acrocentric chromosomes. Single NOR-bearing pair as well as positive heterochromatic blocks at pericentromeric regions were identified in the karyotypes of the three species studied. NOR-bearing sites were positively labeled after Ag-impregnation, C-banding, CMA3-fluorescence and FISH with an 18S rDNA probe but were negative after DAPI-fluorescence. Such remarkable shared conspicuous chromosomal characters corroborate either close phylogenetic relationship of these families, previously established by morphological and molecular data, or rather conservative nature of karyotype differentiation processes. The later hypothesis, however, appears less probable due to centric or in tandem fusions documented for another Balistoidea species. text/html en_US Pensoft Publishers Balistoidea fish cytogenetics karyotype evolution Tetraodontiformes Cytogenetic characterization of three Balistoidea fish species from the Atlantic with inferences on chromosomal evolution in the families Monacanthidae and Balistidae Research Article

10.3897/compcytogen.v5i2.961 2011-07-01 compcytogen

Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Bahia, Ilhéus, Brazil author Menezes, Rodolpho https://orcid.org/0000-0002-6612-3543 Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Bahia, Ilhéus, Brazil author Carvalho, A.F. Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Bahia, ilheus, Brazil author Silva, Janisete https://orcid.org/0000-0002-5570-5484 Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Bahia, Brazil author Costa, M. A. 2011-07-01 2011-07-01 2011 Comparative Cytogenetics 1993-078X 1993-0771 5 71 80 2011 The B chromosome system of Trypoxylon (Trypargilum) albitarse (Hymenoptera, Sphecidae) I. Banding analysis. SMSR Araújo author 2000 text Cytobios 2000 101 7 13 Genetic load caused by variation in the amount of rDNA in a wasp. SMR Araújo author 2002 text Chromosome Research 2002 10 607 613 Karyotypes and heterochromatin distribution (C-band patterns) in three species of Microstigmus wasps (Hymenoptera, Sphecidae, Pemphredoninae). MA Costa author 1993 text Brazilian Journal of Genetics 1993 16 923 926 RE Coville author 1982 Wasps of the genus Trypoxylon subgenus Trypargilum in North America 1982 Myrmecia pilosula, an ant with only one pair of chromosomes MWJ Crosland author 1986 text Science 1986 231 Karyotype characterization of Trigona fulviventris, Guérin, 1835 (Hymenoptera, Meliponini) by C banding and fluorochrome staining: report of a new chromosome number in the genus. AMT Domingues author 2005 text Genetics and Molecular Biology 2005 28 390 393 Occurrence of multiple nucleolus organizer regions and intraspecific karyotype variation in Scaptotrigona xanthotricha Moure (Hymenoptera, Meliponini). OM Duarte author 2009 text Genetics and Molecular Biology 2009 8 3 831 839 Structural dynamics of eukaryotic chromosome evolution. EE Eichler author 2003 text Science 2003 301 793 797 The last twenty years of parasitic Hymenoptera karyology: an update and phylogenetic implications. VE Gokhman author 1995 text Journal of Hymenoptera Research 1995 4 41 63 Chromosomes of parasitic wasps of the genus Metaphycus (Hymenoptera: Chalcidoidea: Encyrtidae). VE Gokhman author 2010 text Comparative Cytogenetics 2010 4 1 21 25 Cytogenetic analysis of three species of Trypoxylon (Trypoxylon) (Hymenoptera, Sphecidae, Larrinae). LF Gomes author 1995 text Brazilian Journal of Genetics 1995 18 173 176 Karyotype evolution in wasps of the genus Trypoxylon (subgenus Trypargilum) (Hymenoptera, Sphecidae). LF Gomes author 1997 text Brazilian Journal of Genetics 1997 20 177 183 Functional genetics and genomics in ants (Hymenoptera: Formicidae): The interplay of genes and social life. MAD Goodisman author 2008 text Myrmecological news 2008 11 107 117 M Guerra author 2002 Como Observar Cromossomos: Um Guia de Técnicas em Citogenética Vegetal Animal e Humana. 2002 The sphecid wasps (Sphecidae) PE Hanson author PE Hanson author 1995 text The Hymenoptera of Costa Rica 1995 621 649 Karyotype evolution in the social wasps (Hymenoptera, Vespidae). H Hoshiba author 1989 text Japanese Journal of Genetics 1989 64 209 222 Chromosome evolution of bees and wasps (Hymenoptera, Apocrita) on the basis of C-banding pattern analyses. H Hoshiba author 1993 text Japanese Journal of Genetics 1993 61 3 465 492 Karyotype evolution in Australian ants. HT Imai author 1977 text Chromosoma 1977 53 341 393 Theoretical bases for karyotype evolution. 1. The minimum-interaction hypothesis. HT Imai author 1986 text The American Naturalist 1986 128 900 920 Modes of spontaneous chromosomal mutation and karyotype evolution in ants with reference to the minimum interaction hypothesis. HT Imai author 1988a text Japanese Journal of Genetics 1988a 63 159 185 Theoretical bases for karyotype evolution. II. The fusion burst in man and mouse. HT Imai author 1988b text Japanese Journal of Genetics 1988b 63 313 342 Chromosomal polymorphisms involving telomere fusion, centromeric inactivation and centromere shift in the ant Myrmecia (pilosula) n = 1. HT Imai author 1989 text Chromosoma 1989 98 456 460 Mutability of constitutive heterocromatin (C-bands) during eukaryotic chromossomal evolution and their cytological meaning. HT Imai author 1991 text Japanese Journal of Genetics 1991 66 653 661 Experimental bases for the minimum interaction theory. I. Chromosome evolution in ants of the Myrmecia pilosula species complex (Hymenoptera: Formicidae: Myrmeciinae). HT Imai author 1994 text Japanese Journal of Genetics 1994 69 137 182 A GC-rich satellite DNA and karyology of the bivalve mollusk Donax trunculus: a dominance of GC-rich heterochromatin. V Petrović author 2009 text Cytogenetic and Genome Research 2009 124 63 71 Cytogenetics of Brazilian Polybiini wasps (Hymenoptera, Vespidae, Polistinae). SG Pompolo author 1987 text Brazilian Journal of Genetics 1987 10 483 496 Chromosome numbers and C-banding in two wasp species of the genus Polistes (Hymenoptera, Polistinae, Polistini). SG Pompolo author 1990 text Insectes Sociaux 1990 37 251 257 DNA characterization and karyotypic evolution in the bee genus Melipona (Hymenoptera, Meliponini). MP Rocha author 2002 text Hereditas 2002 136 19 27 Phylogeny of the Hymenoptera (Insecta): The state of the art. F Ronquist author 1999 text Zoologica Scripta 1999 28 1–2 3 11 Phylogenomic analysis reveals bees and wasps (Hymenoptera) at the base of the radiation of Holometabolous insects. J Savard author 2006 text Genome Research 2006 16 1334 1338 Evolutionary dynamics of the karyotype of the wasp Trypoxylon (Trypargilum) nitidum (Hymenoptera, Sphecidae) from the Rio Doce state Park, Minas Gerais, Brazil. R Scher author 2003 text Genetics and Molecular Biology 2003 26 307 311 Simultaneous fluorescent staining of R bands and specific heterochromatic regions (DA-DAPI bands) in human chromosomes. D Schweizer author 1980 text Cytogenetics and Cell Genetics 1980 27 190 193 The evolution of CMA bands in Citrus and related genera. AEB Silva author 2010 text Chromosome Research 2010 18 503 514 A simple technique for demonstration of centromeric heterochromatin. AT Sumner author 1972 text Experimental Cell Research 1972 75 304 306 10.3897/compcytogen.v5i2.961 https://compcytogen.pensoft.net/article/1719/ https://compcytogen.pensoft.net/article/1719/download/pdf/ Previous cytogenetic analyses in Trypoxylon Latreille, 1796 have been basically restricted to C-banding. In the present study, base-specific CMA3 and DAPI fluorochrome staining were used to characterize the constitutive heterochromatin in three Trypoxylon species. The heterochromatin was GC-rich in all the species studied; however, in Trypoxylon nitidum F. Smith, 1856 the molecular composition of the heterochromatin was different among chromosome pairs. Conversely, the euchromatin was AT-rich in the three species. These results suggest high conservatism in the euchromatic regions as opposed to the heterochromatic regions that have a high rate of changes. In this study, we report the karyotype of Trypoxylon rugifrons F. Smith, 1873 which has the lowest chromosome number in the genus and other characteristics of the likely ancestral Trypoxylon karyotype. text/html en_US Pensoft Publishers Comparative cytogenetics heterochromatin CMA3/DAPI Molecular characterization of constitutive heterochromatin in three species of Trypoxylon (Hymenoptera, Crabronidae, Trypoxylini) by CMA3/DAPI staining Research Article

10.3897/compcytogen.v5i2.1151 2011-07-01 compcytogen

Institute of Vertebrate Biology, Academy of Sciences of the Czech republic, Brno, Czech Republic author Zima, Jan Department of Biology, Faculty of Science and Arts, Selcuk University, author Arslan, Atilla https://orcid.org/0000-0002-4766-4969 2011-07-01 2011-07-01 2011 Comparative Cytogenetics 1993-078X 1993-0771 5 81 89 2011 Standard G-, Q-, and R-banded ideograms of the domestic sheep (Ovis aries): homology with cattle (Bos taurus). Report of Committee for the standardization of the sheep karyotype. HA Ansari author 1999 text Cytogenetics and Cell Genetics 1999 87 134 142 Translocations of acrocentric chromosomes and their implications in the evolution of sheep (Ovis). TD Bunch author 1976 text Cytogenetics and Cell Genetics 1976 17 122 136 Chromosome number of Severtsov’s sheep (Ovis ammon severtsovi): G-banded karyotype comparisons within Ovis. TD Bunch author 1998 text Journal of Heredity 1998 89 266 269 Cytogenetics, morphology and evolution of four subspecies of the giant sheep argali (Ovis ammon) of Asia. TD Bunch author 2000 text Mammalia 2000 64 199 207 RI Freshney author 1990 Culture of animal cells. A manual of basic technique. 2nd ed 1990 P Grubb author 2005 Order Artiodactyla. Wilson DE, Reeder DM (Eds). In: Mammal species of the world. A taxonomic and geographical reference. Volume 1. 3rd Ed 2005 637 722 Molecular analysis of wild and domestic sheep questions current nomenclature and provides evidence for domestication from two different subspecies S Hiendleder author 2002 text Proceedings of the Royal Society London 2002 B269 893 904 The complete mitochondrial DNA sequence of the domestic sheep (Ovis aries) and comparison with the other major ovine haplotypes. S Hiendleder author 1998a text Journal of Molecular Evolution 1998a 47 441 448 Analysis of mitochondrial DNA indicates that domestic sheep are derived from two different ancestral maternal sources. No evidence for contribution from urial and argali sheep. S Hiendleder author 1998b text Journal of Heredity 1998b 89 113 120 Controlled silver staining of nucleolar organizer regions with a protective colloidal developer: a 1-step method WM Howell author 1980 text Experientia 1980 36 1014 1015 International system for chromosome nomenclature of domestic bovids. author 2000 text Cytogenetics and Cell Genetics 2000 92 283 299 The morphology, increase in weight, development of teeth and horns in Anatolian wild sheep, Ovis orientalis anatolica Valenciennes, 1856, living in Bozdağ Konya MA Kaya author 1991 text Turkish Journal of Zoology 1991 15 135 149 General characteristics and taxonomy of the Turkish mouflon Ovis gmelini anatolica Valenciennes, 1856 MA Kaya author A Náhlik author 2000 text International Mouflon Symposium, October 27–29, 2000 2000 92 95 On Konya wild sheep, Ovis orientalisanatolica, in the Bozdag protected area MA Kaya author 2004 text Mammalia 2004 68 229 232 A study on the chromosomes of Konya wild sheep (Ovis orientalis spp.): Case report K Kırıkçi author 2003 text Turkish Journal of Veterinary and Animal Sciences 2003 27 281 283 Chromosomes of the Siberian snow sheep, Ovis nivicola, and implications concerning the origin of amphiberingian wild sheep (subgenus Pachyceros). KV Korobitsyna author 1974 text Quaternary Research 1974 4 235 245 B Kryštufek author 2009 Mammals of Turkey and Cyprus. Rodentia II. Cricetinae, Muridae, Spalacidae, Calomyscidae, Capromyidae, Hystricidae, Castoridae 2009 Chromosomal sets and taxonomical position of Ovis ammon severtzovi Nasonov EA Lyapunova author 1997 text Zoologicheskii Zhurnal (Zoological journal) 1997 76 1083 1093 Cytogenetic differentiation, geographic distribution and domestication of Palaearctic sheep (Ovis). CF Nadler author 1973a text Zeitschrift für Säugetierkunde 1973a 38 109 125 G-band patterns as chromosomal markers, and the interpretation of chromosomal evolution in wild sheep (Ovis). CF Nadler author 1973b text Experientia 1973b 29 117 119 A rapid banding technique for human chromosomes M Seabright author 1971 text Lancet 1971 2 971 972 . Turkish mouflon (Ovis gmelini anatolica) in Central Anatolia: Population viability under scenarios of harvesting for trophy Z Sezen author HR Akcakaya author 2004 text Species Conservation and Management: Case Studies. Section VI, Chapter 41, Mammals 2004 459 467 DM Shackleton author 1997 Wild sheep and goats and their relatives. Status survey and conservation action plan for Caprinae 1997 The karyotype and chromosome banding in mouflon in the Czech Republic. M Slavíčková author 1985 text Veterinary Medicine (Czech) 1985 30 681 686 A simple technique for demonstrating centromeric heterochromatin. AT Sumner author 1972 text Experimental Cell Research 1972 75 304 306 Evolution of wild sheep in Iran. R Valdez author 1978 text Evolution 1978 32 56 72 Cytogenetic differentiation and species borders in true Palaearctic sheeps (Ovis s. str.) NN Vorontsov author 1972a text Zoologicheskii Zhurnal 1972a 51 1109 1123 Chromosomi dikich baranov i proiskhozhdenie domashnikh ovets NN Vorontsov author 1972b text Priroda 1972b 3 74 82 Comparative geometrical morphometries on the mandibles of Anatolian wild sheep (Ovis gmelini anatolica) and Akkaraman sheep (Ovis aries). H Yalçın author 2010 text Kafkas Universitesi Veterinarer Fakultesi Dergisi 2010 16 55 61 10.3897/compcytogen.v5i2.1151 https://compcytogen.pensoft.net/article/1718/ https://compcytogen.pensoft.net/article/1718/download/pdf/ The karyotype, C-banding, and nucleoar organizer regions (NORs) of eight specimens of Konya wild sheep from Turkey were examined. The complement included six large metacentric autosomes, 46 acrocentric autosomes of decreasing size, a medium-sized acrocentric X chromosome, and a small bi-armed Y chromosome (the diploid chromosome number 2n=54, the number of autosomal arms NFa=58, the number of chromosome arms NF=61). G-banding allowed reliable identification of all the chromosome pairs and the pairing of homologous elements. All the autosomes possessed distinct centromeric or pericentromeric C-positive bands. The X chromosome had a pericentromeric C-positive band, and the Y chromosome was entirely C-heterochromatic. The NORs were located in the terminal regions of the long arms of three metacentric and two acrocentric autosomes. The karyotype of the Konya wild sheep and its banding patterns are quite similar to chromosome complement reported in domestic sheep and European mouflon. text/html en_US Pensoft Publishers cytogenetics Ovis sheep domestication Turkey Banded karyotype of the Konya wild sheep (Ovis orientalis anatolica Valenciennes, 1856) from Turkey Research Article

10.3897/compcytogen.v5i2.968 2011-07-01 compcytogen

Ivan Franko Zhytomyr State University, Zhytomyr, Ukraine author Vlasenko, R.P. Schmalhausen Institute of Zoology NAS of Ukraine, Kiev, Ukraine author Меzhzherin, S Ivan Franko Zhytomyr State University, Zhytomyr, Ukraine author Garbar, A Ivan Franko Zhytomyr State University, Zhytomyr, Ukraine author Kotsuba, I. Yu. 2011-07-01 2011-07-01 2011 Comparative Cytogenetics 1993-078X 1993-0771 5 91 103 2011 UB Byzova author 1987 Quantitative methods in the soil zoology 1987 A Cywinska author 2002 text Journal of Evolutionary Biology 2002 15 1 134 142 Karyotypes of three species of the genus Aporrectodea Örley (Oligochaeta: Lumbricidae) from the Ukraine. AV Garbar author 2007 text Comparative Cytogenetics 2007 1 1 59 62 On the enolution of clonal diversity in parthenogenetic earthworms. J Jaenike author 1982 text Pedobiologia 1982 23 4 304 309 Genetic polymorphism and evolution in parthenogenetic animals. XI. Genetic differention in parthenogenetic populations. J Lokki author 1980 text Hereditas 1980 92 275 278 Genetic structure and morphological peculiarities of Aporrectodea (superspecies) caliginosa (Oligochaeta: Lumbricidae) on the territory of Ukraine SV Mezhzherin author 2007 text Procedings of the III International Oligochaete Taxonomy Meeting. Platres, Cyprus, April 2- 6, 2007 2007 The analysis of clone diversity of two apomictic earthworm species (Lumbricidae: Aporrectodea) and the problems of large and small specimens variability SV Mezhzherin author 2007 text Reports of National Academy of Sciences of Ukraine 2007 8 151 156 The chromosomes of the earthworms I. The evolution of polyploidy. S Muldal author 1952 text Heredity 1952 6 55 76 Caryology of the Lumbricidae. P Omodeo author 1952 text Caryologia 1952 4 173 275 Serum protein electrophoresis in acrilamyde gel patterns from normal human subjects. FC Peacock author 1965 text Science 1965 147 1451 1455 TS Perel author 1979 Range and regularities in the distribution of earthworms of the USSR fauna 1979 New results of researches of Benedictia (Gastropoda, Pectinibranchia, Benedictiidae) TJ Sitnikova author AA Linevich author 1990 text Morphology and evolution of invertebrates. Collected papers 1990 266 281 Genetic and morphological variation of parthenogenetic earthworm Aporrectodea rosea in southern Finland (Oligochaeta, Lumbricidae). J Terhivuo author 1993 text Annales Zoologici Fennici 1993 30 13 215 224 Clone pool structure and morphological variation in endogeic and epigeic North-European parthenogenetic earthworm (Oligochaeta: Lumbricidae). J Terhivuo author 1996 text Pedobiologia 1996 40 3 226 239 Dispersal and clonal diversity of North-European parthenogenetic earthworms. J Terhivuo author 2006 text Biological Invasions 2006 8 1205 1218 A Vedovini author 1973 Systematique, caryologie et ecologie des oligochetes terrestres de la region Provençale 1973 Polyploid race variety in the earthworms family Lumbricidae AG Victorov author 1993 text Uspekhi sovremennoĭ biologii 1993 113 3 304 312 Clonal structure, karyological and morphological analysis of the isolated settlement of hipervariable earthworms species Aporrectodea rosea (Oligochaeta: Lumbricidae) RP Vlasenko author 2007 text Science Bulletin of Uzhgorod University. Series biological 2007 21 187 191 TS Vsevolodova-Perel author 1997 The earthworms in the fauna of Russia. Cadastre and the key 1997 Polyploid races of earthworms (Lumbricidae, Oligochaeta) in the East European Plain and Siberia. TS Vsevolodova-Perel author 2008 text Biological Bulletin 2008 35 4 385 388 10.3897/compcytogen.v5i2.968 https://compcytogen.pensoft.net/article/1717/ https://compcytogen.pensoft.net/article/1717/download/pdf/ Four chromosomal races (2n=36, 3n=54, 6n=108, 8n=144) and 96 clones have been revealed among 224 specimens of the earthworm A. rosea over the territory of Ukraine by means of karyological analysis and biochemical genetic marking. Each population has been showed by several clones at least; moreover the clones from different places have never been identical. The clones in the range of one population can be identified with the set of quantitative and qualitative parameters. text/html en_US Pensoft Publishers earthworms Aporrectodea rosea polyploidy genetic structure morphology Polyploid races, genetic structure and morphological features of earthworm Aporrectodea rosea (Savigny, 1826) (Oligohaeta, Lumbricidae) in Ukraine Research Article

10.3897/compcytogen.v5i2.969 2011-07-01 compcytogen

Necmettin Erbakan University, Faculty of Science, Department of Biotechnology, Konya, Konya, author Martin, Esra Harran University, Faculty of Science and Arts, Department of Biology, Sanlıurfa, author Akan, H. Gazi University, Faculty of Science and Arts, Department of Biology, Ankara, author Ekici, M. Gazi University, Faculty of Science and Arts, Department of Biology, Ankara, author Aytac, Z. 2011-07-01 2011-07-01 2011 Comparative Cytogenetics 1993-078X 1993-0771 5 105 121 2011 Cytological studies in the genus Trigonella L. K Agarwal author 1983 text Cytologia 1983 48 771 779 Effects of fenugreek seeds and its extracts on plasma lipid profile: A study on rabbits. M Al-Habori author 1998 text Phytotherapy Research 1998 12 572 575 SB Astanova author 1981 Chromosome numbers of Leguminosae of Flora in Tajikistan, (pp. 24: 61- 63) Dokl. Akad. Nauk Tadziksk. SSR. 1981 Ş Bal author 1990 Trigonella foeanum-graecum’da karyotip analizleri. 1990 A classification of the generic limits of Trigonella and Medicago. BR Baum author 1968 text Canadian Journal of Botany 1968 46 741 749 Inter-and intraspecific chromosomal variations in four species of Trigonella L. L Bidak author 1996 text Journal Union Arab Biology 1996 3 203 215 A phenolic-taxometric study of Medicago (Leguminosae). D Classen author 1982 text Canadian Journal of Botany 1982 60 2477 2495 Contributions to the flora of Israel and Sinae. V. Trigonella sibthorpii Boiss., a new record from Israel. A Danin author 1989 text Israel Journal of Botany 1989 38 121 124 CD Darlington author 1955 Chromosome Atlas of Flowering Plants. 1955 AJ Duke author 1986 Handbook of Legumes of World Economic Importance. 1986 Chromosome number of Trigonella spinosa. AK Ghosh author 1980 text Current Science 1980 49 154 155 Trigonella L. In Davis PH (ed.) Flora of Turkey and the East Aegean Islands. A Huber-Murath author 1970 text Edinburgh University Press 1970 3 452 482 Localization and activity of rRNA genes on fenugreek(Trigonella foenum-graecum L.) chromosomes by fluorescent in situ hybridization and silver staining. G Ladizinsky author 1986 text Plant Systematics and Evolution: 1986 1 5 DJ Mabberly author 1997 The plant-book: A Portable Dictionary of the Higher Plants. 1997 Karyomorphological studies on section Bucerates Boiss. of Trigonella L. (Leguminosae) from Turkey. E Martin author 2008 text Caryologia 2008 61 225 236 Über die Verbreitung einiger Papilionacean-Gattun. H Meusel author 1962 text Kulturpflanze Beih 1962 3 249 262 Medicinal foodstuffs. 17. Fenugreek seed. (3): Structures of new furostanol-type steroid saponins, trigoneosides 10a, 10b, 11b, 12a, 12 bands 13a from the seeds of Egyptian Trigonella foenum-graceum L. T Murakami author 2000 text Chemical Pharmaceutical Bulletin 2000 48 994 1000 R Oncina author 2000 Bioproduction of diosgenin incallus cultures of Trigonella foenum-graecum L.Departemento de Biologia Vegetal, Facultad de Biologia, Universidad de Murcia, Campus de Espinardo, Murcia, Spain. 2000 Mediterranean chromosome number reports 6 (762–766). D Pavlova author 1996 text Flora Mediterranea 1996 6 323 328 GA Petropoulos author 2002 Fenugreek, the Genus Trigonella, Medicinal and Aromatic Plants-Indutsrial Profiles. 2002 Effect of level of phosporus and potash on the performance of seedvield of “Pusa Early bunching” fenugreek(Trigonella foenum-graceum). D Ram author 2000 text Indian Journal of Agriculture 2000 12 866 868 Seperation and characterisation of fenugreek seeds proteins. DM Sabir author 2002 text Deut lebensm-rundsch 2002 1 14 16 Thermal efficiency of fenugreek(Trigonella foenum-graceum) genotypes under different sowing environments and phosphoruslevels. RS Sheoran author 1999 text Indian Journal of Agriculture 1999 12 830 832 Die Entwicklungsgeschichte der Gattung Trigonella (Suppl. Ad “Generis Trigonella revisio critica”). Bull. Assoc. Russe. Rech. Sci. G Sırjaev author 1935 text Prague 1935 9 135 162 Pollen-ovule patterns in tribe Trifolieae (Leguminosae). E Small author 1988 text Plant Systematics of Evolution 1988 160 195 205 The taxonomic value of floral characters in tribe Trigonelleae (Leguminosae), with special reference to Medicago. E Small author 1981 text Canadian Journal of Botany 1981 59 1578 1598 A note on Trigonella (Melilotus) bicolor. E Small author 1989 text Canadian Journal of Botany 1989 67 1604 1606 Remarkable asymmetries in trifoliolate leaves with particular reference to Medicago. E Small author 1981 text Canadian Journal of Botany 1981 59 662 671 Trigonella foenum graceum (Fenugreek) Seed extract as an Antineoplastic Agent. P Sur author 2001 text Phytotherapy Research 2001 3 257 259 TG Tutin author 1964 Flora Europaea, I & II. 1964 Karyological study on six Trigonella L. species (Leguminosae) in Turkey. A Yılmaz author 2009 text Caryologia 2009 62 89 94 10.3897/compcytogen.v5i2.969 https://compcytogen.pensoft.net/article/1716/ https://compcytogen.pensoft.net/article/1716/download/pdf/ Karyotypes of ten sections of genus Trigonella Linnaeus, 1753 (Fabaceae) from Turkey were investigated. Somatic chromosome numbers of examined species were determined as 2n=14 and 16. The karyotype analyses of the species were carried out and somatic chromosome numbers of Trigonella plicata Boiss., 1872, T. brachycarpa (Fisch.) Moris, 1833, T. rostrata Boiss., 1872, T. lunata Boiss., 1843, T. isthmocarpa Boiss. et Balansa 1856, T. rhytidocarpa Boiss. et Balansa, 1859, T. spicata Sibth. et Sm., 1813, T. cephalotes Boiss. et Balansa 1856, T. capitata Boiss., 1843 and T. gladiata Steven, 1808 were reported for the first time. Two pairs of satellite metaphase chromosomes were observed in T. cariensis Boiss., 1843 and one pair in T. lunata. Moreover, 2 B-chromosomes were found only in T. procumbens Rchb., 1830 among all studied species. The aims of this study are to provide karyological data for a significant pool of the taxa, to show differences among them in the number, size and morphology of somatic chromosomes, to verify previous reports or represent numbers which are different from those cited previously. text/html en_US Pensoft Publishers Somatic chromosome Turkey Fabaceae karyotype Karyotype analysis of ten sections of Trigonella (Fabaceae) Research Article

10.3897/compcytogen.v5i2.960 2011-07-01 compcytogen

Departamento de Genética, Centro de Ciências Biológicas/CCB, Universidade Federal de Pernambuco/ UFPE, Pernambuco, Brazil author Carvalho, D.B. Instituto de Ciências Biológicas/ICB, Universidade de Pernambuco /UPE, Recife, Pernambuco, Brazil author Rocha, M.F. Departamento de Genética, Centro de Ciências Biológicas/CCB, Universidade Federal de Pernambuco/ UFPE, Pernambuco, Brazil author Loreto, V. Departamento de Botânica, Centro de Ciências Biológicas/CCB, Universidade Federal de Pernambuco/ UFPE, Brazil author Silva, A.E.B. Departamento de Genética, Centro de Ciências Biológicas/CCB, Universidade Federal de Pernambuco/ UFPE, Pernambuco, Brazil author Souza, M. J. 2011-07-01 2011-07-01 2011 Comparative Cytogenetics 1993-078X 1993-0771 5 123 132 2011 Controlled silver staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. WM Howell author 1980 text Experientia 1980 36 1014 1015 Karyotype, constitutive heterochromatin and nucleolar organizer regions in Belosacris coccineipes (Acrididae-Leptysminae). V Loreto author 2000 text Genetic and Molecular Biology 2000 23 1 5 A comparative cytogenetic analysis between the grasshopper species Chromacris nuptialis and C. speciosa (Romaleidae): constitutive heterochromatin variability and rDNA sites. V Loreto author 2005 text Genetica 2005 125 253 260 Comparative analysis of rDNA location in five Neotropical gomphocerine grasshopper species. V Loreto author 2008 text Genetica 2008 132 95 101 Cytological studies in the family Ommexechidae (Orthoptera, Ommexechidae). A Mesa author 1977 text Acrida 1977 6 261 271 Cariologia de los Acridoideos Neotropicales: Estado actual de su conocimiento y nuevas contribuciones. A Mesa author 1982 text Annals Society Entomology of France 1982 18 507 526 The karyotype of the grasshopper Spathalium helios Rehn 1918. (Orthoptera, Acridoidea, Ommexechidae). A Mesa author 1990 text Revista Brasileira de Genética 1990 13 705 710 The use of combined FISH/GISH in conjunction with DAPI counterstaining to identify chromosomes containing transgene inserts in amphidiploids tobacco. EA Moscone author 1996 text Chromosoma 1996 105 231 236 Nature and distribution of constitutive heterochromatin and NOR location in the grasshopper Phaeoparia megacephala (Romaleidae, Orthoptera). LG Pereira author 2000 text Cytobios 2000 103 101 119 Karyotype variability in the genus Radacridium (Orthoptera, Romaleidae, Romaleinae). MF Rocha author 1997 text Cytologia 1997 62 53 60 Karyotypic analysis, constitutive heterochromatin and NOR distribution in five grasshopper species of the subfamily Leptysminae (Acrididae). MF Rocha author 2004 text Caryologia 2004 57 107 116 La familia Ommexechidae (Orthoptera, Acridoidea). RA Ronderos author 1979 text Acrida 1979 8 241 273 NOR and nucleolus in the spermatogenesis of acridoid grasshoppers. JS Rufas author 1985 text Genetica 1985 66 139 144 Variability of constitutive heterochromatin in karyotypes of representatives of the family Romaleidae (Orthoptera). MJ Souza author 1995 text Brazilian Journal of Genetics 1995 18 517 520 Constitutive heterocrhomatin, NOR localition and FISH in the grasshopper Xyleus angulatus (Romaleidae). MJ Souza author 1998 text Caryologia 1998 51 73 80 Karyotype, C-and fluorescence banding patterns NOR location and FISH in the grasshopper Xestotrachelus robustus (Romaleidae). MJ Souza author 2003 text Caryologia 2003 56 261 267 Chromosome study in Schistocerca (Orthoptera- Acrididae-Cyrtacanthacridinae): Karyotypes and distribution patterns of constitutive heterochromatin and nucleolus organizer regions (NORs). MJ Souza author 2007 text Genetic and Molecular Biology 2007 30 54 59 A simple technique for demostrating centromeric heterochromatin. AT Sumner author 1972 text Experimental Cell Research 1972 75 304 306 Cytogenetics of the parthenogenetc grasshopper Warramba virgo and its bisexual relatives. X. Pattern of fluorescent banding. D Schweizer author 1983 text Chromosoma 1983 88 227 236 10.3897/compcytogen.v5i2.960 https://compcytogen.pensoft.net/article/1715/ https://compcytogen.pensoft.net/article/1715/download/pdf/ Chromosomes of Ommexecha virens and Descampsacris serrulatum (Ommexechidae) were analyzed through conventional staining, C-banding, base specific fluorochromes, silver nitrate impregnation (AgNO3), and fluorescent in situ hybridization (FISH) with probe for 45S rDNA. The two species presented diploid number 2n= 23,X0 in males and acrocentric autosomes, except the pair one that presented submetacentric morphology. The X chromosome has distinct morphology in the two analyzed species, being a medium acrocentric in O. virens and large submetacentric in D. serrulatum. The C-banding revealed pericentromeric blocks of constitutive heterochromatin (CH) in all the chromosomes of D. serrulatum. For O. virens it was evidenced that the blocks of CH are preferentially located in the pericentromeric area (however some bivalents presents additional blocks) or in different positions. The staining with CMA3/DA/DAPI showed GC rich CH blocks (CMA3+) in some chromosomes of the two species. The nucleolar organizer regions (NORs) were located in the bivalents L2, S9, S10 of O. virens and M5, M6, M7, S11 of D. serrulatum. The FISH for rDNA showed coincident results with the pattern of active NORs revealed by AgNO3. This work presents the first chromosomal data, obtained through differential cytogenetics techniques in Ommexechidae, contributing to a better characterization of karyotypic evolution for this grasshopper family. text/html en_US Pensoft Publishers FISH grasshopper Ommexechidae rDNA Ommexecha virens Descampsacris serrulatum Ommexecha virens (Thunberg, 1824) and Descampsacris serrulatum (Serville, 1831) (Orthoptera, Ommexechidae): karyotypes, constitutive heterochromatin and nucleolar organizing regions Research Article

10.3897/compcytogen.v5i2.964 2011-07-01 compcytogen

Universidade Federal de São Carlos. Depto. Genética e Evolução. São Carlos, Brazil author Mendes-Neto, Ernani de Oliveira Universidade Estadual de Ponta Grossa. Depto. Biologia Estrutural, Molecular e Genética. Ponta Grossa, Brazil author Vicari, Marcelo Ricardo Universidade Estadual de Ponta Grossa. Depto. Biologia Estrutural, Molecular e Genética. Ponta Grossa, Ponta Grossa, Brazil author Artoni, Roberto Universidade Federal de São Carlos. Depto. Genética e Evolução. São Carlos, Brazil author Moreira-Filho, Orlando 2011-07-01 2011-07-01 2011 Comparative Cytogenetics 1993-078X 1993-0771 5 133 142 2011 Karyotypic relationships among the tribes of Hypostominae (Siluriformes: Loricariidae) with description of X0 sex chromosome system in a Neotropical fish species. AL Alves author 2006 text Genetica 2006 128 1 9 Phylogenetic relationships of the suckermouth armoured catfishes (Loricariidae) with emphasis on the Hypostominae and the Ancistrinae. JW Armbruster author 2004 text Zoological Journal of the Linnean Society 2004 141 1 80 Cytogenetic studies on Hypostominae (Pisces, Siluriformes, Loricariidae). Considerations on karyotype evolution in the genus Hypostomus. RF Artoni author 1996 text Caryologia 1996 49 81 90 Nature and distribution of constitutive heterochromatin in fishes, genus Hypostomus (Loricariidae). RF Artoni author 1999 text Genetica 1999 106 209 214 Trends in the karyotype evolution of Loricariidae fish (Siluriformes). RF Artoni author 2001 text Hereditas 2001 134 201 210 Heterochromatin analysis in the fish species Liposarcus anisitsi (Siluriformes) and Leporinus elongatus (Characiformes). RF Artoni author 1999 text Genetics and Molecular Biology 1999 22 1 6 Astyanax aff. fasciatus Cuvier, 1819 (Teleostei; Characidae): evidences of a species complex in the upper rio Tibagi basin (Paraná, Brazil). RF Artoni author 2006 text Neotropical Ichthyology 2006 4 2 197 202 Karyotype diversity and fish conservation of southern field from South Brazil. RF Artoni author 2009 text Review in Fish Biology and Fisheries 2009 19 393 401 Cytotaxonomic considerations on Hoplias lacerdae (Pisces, Erythrinidae). LAC Bertollo author 1978 text Brazilian Journal of Genetics 1978 1 2 103 120 Two new species of the genus Hemiancistrus Bleeker (Teleostei: Siluriformes: Loricariidae) from the upper Rio Uruguai basin. AR Cardoso author 2004 text Neotropical Ichthyology 2004 2 1 1 8 Família Loricariidae: Hypostominae M Carvalho author PA Buckup author 2007 text Catálogo das espécies de peixes de água doce do Brasil 2007 91 98 Karyotype description of two species of Hypostomus (Siluriformes, Loricariidae) of the Planalto da Bodoquena, Brazil. SS Cereali author 2008 text Genetics and Molecular Research 2008 7 3 583 591 Checklist of catfishes, recent and fossil (Osteichthyes: Siluriformes), and catalogue of siluriform primary types. Jr. Ferraris CJ author 2007 text Zootaxa 2007 1418 1 628 Mapping of the 18S and 5S ribosomal RNA genes in the fish Prochilodus argenteus, Agassiz, 1829 (Characiformes, Prochilodontidae). TE Hatanaka author 2004 text Genetica 2004 122 239 244 Controled silver-staining of nucleolus organizer regions with the protective coloidal developer: a 1-step method. WM Howell author 1980 text Experientia 1980 36 1014 1015 Classification and catalogue of the mailed Loricariidae (Pisces, Siluriformes). IJH Isbrücker author 1980 text Verslagen en Technische Gegevens, Universiteit van Amsterdan 1980 22 1 181 Karyotypic diversity and evolution of Loricariidae (Pisces, Siluriformes). KF Kavalco author 2005 text Heredity 2005 94 180 186 Nomenclature for centromeric position of chromosomes. A Levan author 1964 text Hereditas 1964 52 201 220 Chromosomal localization of 5S rDNA genes in Leporinus fish (Anostomidae, Characiformes). C Martins author 1999 text Chromosome Research 1999 7 363 367 Different cytotypes in fishes of the genus Hypostomus Lacépède, 1803, (Siluriformes: Loricariidae) from Xingu river (Amazon region, Brazil). SSR Milhomem author 2010 text Comparative Cytogenetics 2010 4 45 54 Historical biogeography of the catfish genus Hypostomus (Siluriformes: Loricariidae), with implications on the diversification of Neotropical ichthyofauna. JI Montoya-Burgos author 2003 text Molecular Ecology 2003 12 1855 1867 A poorly known case of watershed transposition between the São Francisco and upper Paraná river basins. O Moreira-Filho author 2005 text Neotropical Ichthyology 2005 3 3 449 452 On the diploid state of the fish order Ostariophys. JI Muramoto author 1968 text Chromosoma 1968 24 59 66 Mechanisms of chromosomal evolution and its possible relation to natural history characteristics in Ancistrus catfishes (Siluriformes: Loricariidae). RR Oliveira author 2009 text Journal of Fish Biology 2009 75 2209 2225 Genetic variability of Hypostomus (Teleostei, Loricariidae) from the Ribeirão Maringá, a stream of the upper Rio Paraná basin, Brazil. S Paiva author 2005 text Genetics and Molecular Biology 2005 28 370 375 Isbrueckerichthys epakmos, a new species of loricariid catfish from the rio Ribeira de Iguape basin, Brazil (Teleostei: Siluriformes). EHL Pereira author 2003 text Neotropical Ichthyology 2003 1 1 3 9 Cytogenetic analysis using quantitative, high-sensitivity, fluorescent hybridization. D Pinkel author 1986 text Proceedings of the National Academy of Sciences 1986 83 2934 2938 RE Reis author 2003 Check list of the freshwater fishes of South and Central America 2003 Delturinae, a new loricariid catfish subfamily (Teleostei, Siluriformes), with revision of Delturus and Hemipsilichthys. RE Reis author 2006 text Zoological Journal of the Linnean Society 2006 147 277 299 A simple technique for demonstrating centromeric heterocromation. AT Sumner author 1972 text Experimental Cell Research 1972 75 304 306 Subfamily Hypostominae (Armored Cartfishes) C Weber author RE Reis author 2003 text Check list of the freshwater fishes of South and Central America 2003 351 372 10.3897/compcytogen.v5i2.964 https://compcytogen.pensoft.net/article/1714/ https://compcytogen.pensoft.net/article/1714/download/pdf/ The paper represents a comparative cytogenetic analysis of three populations of Hypostomus regani in Brazil. Two populations belong to the Upper Paraná River Basin and the third one, the karyotype of which is described for the first time, was probably introduced into the São Francisco River Basin through transposition from the Piumhi River. Karyotype features of populations of H. regani from the Piracicaba and Tietê River Basins are also discussed. The occurrence of H. regani in the São Francisco River Basin is reported for the first time here. The study also revealed distinct differences in the location of the Ag-NORs between the analyzed populations that enable individuals from the Piumhi River, Mogi-Guaçu River and Tietê River to be distinguished from one another. Thus, the data obtained indicate the possibility of geographic variation fixing different karyotypes even in the same basin of origin. text/html en_US Pensoft Publishers citotaxonomy karyotype diversification rDNA Description of karyotype in Hypostomus regani (Ihering, 1905) (Teleostei, Loricariidae) from the Piumhi river in Brazil with comments on karyotype variation found in Hypostomus Research Article

10.3897/compcytogen.v5i3.1359 2011-08-24 compcytogen

Punjabi University, Patiala, Patiala, India author Kumar, Puneet https://orcid.org/0000-0002-7674-006X PUNJABI UNIVERSITY, Patiala, India author Singhal, Vijay 2011-08-24 2011-08-24 2011 Comparative Cytogenetics 1993-078X 1993-0771 5 143 161 2011 Morphological variation and distribution of cytotypes in the diploid-tetraploid complex of the genus Dactylis L. (Poaceae) from Algeria N Amirouche author 2007 text Plant Systematics and Evolution 2007 264 157 174 Beiträge zur morphologie der gymnospermen. IV. Was sind die “Keimbläschen” oder “Hofmeisters-Körperchen” in der Eizelle der Abietineen?. W Arnoldy author 1900 text Flora 1900 87 194 204 BS Aswal author 1994  Flora of Lahaul-Spiti (a cold desert in northwest Himalaya). 1994 Cytomixis in pollen mother cells of Papaver dubium L. JR Bahl author 1988 text Cytologia 1988 53 771 775 Cytomixis in pollen mother cells of Urochloa panicoides P. Beauv. (Poaceae). D Basavaiah author 1987 text Cytologia 1987 52 69 74 Cytomixis in pollen mother cells of Medicago sativa L M Bellucci author 2003 text Journal of Heredity 2003 94 512 516 SOCGI plant chromosome number reports-II. SS Bir author 1984 text Journal of Cytology and Genetics 1984 19 114 115 Chromosomal studies in certain members of Ranunculaceae and Menispermaceae. SS Bir author 1987 text Proceedings of the Indian Science Congress Association 1987 74 184 185 Cytomixis in maize microsporocytes. CM Caetano-Pereira author 1997 text Cytologia 1997 62 351 355 Genome size variation and morphological differentiation within Ranunculus parnassifolius group (Ranunculaceae) from calcareous screes in the Northwest of Spain E Cires author 2009 text Plant Systematics and Evolution 2009 281 193 208 CD Darlington author 1955 Chromosome altas of flowering plants. 1955 DS Dhaliwal author 1999 Flora of Kullu district (Himachal Pradesh). 1999 E Falistocco author 1995 text Journal of Heredity 1995 86 448 453 Pollen formation in Oenothera gigas. RR Gates author 1911 text Annals of Botany 1911 25 909 940 P Goldblatt author 1981, 1984, 1985, 1988 Index to plant chromosome numbers  1975–1978, 1979–1981, 1982–1983, 1984–1985. Monographs in Systematic Botany from the Missouri Botanical Garden USA 1981, 1984, 1985, 1988 5, 8, 13, 23 Ecological, morphological and allozymic differentiation between diploid and tetraploid knapweeds (Centaurea jacea) from a contact zone in the Belgian Ardenes OJ Hardy author 2000 text New Phytologist 2000 146 281 290 Cytomixis in pollen mother cells of Polygonum tomentosum Schrank. SA Haroun author 1995 text Cytologia 1995 60 257 260 Cytomixis in the microsporogenesis of Vicia faba L. (Fabaceae) SA Haroun author 2004 text Cytologia 2004 69 7 11 Impact of chromatin transfer and spindle abnormalities on pollen fertility and pollen size in Plantago lanceolata L P Himshikha, Kumar author 2010 text Cytologia 2010 75 421 426 PK Holmgren author 1998 Index Herbariorum: A Global Directory of Public Herbaria and Associated Staff. New York Botanical Garden’s Virtual Herbarium 1998 Ethnobotany of certain medicinal plants used by tribals of India against skin diseases. RS Iyer author 1992 text Ancient Science of Life 1992 11 143 152 SK Jain author 1991 Dictionary of Indian Folk Medicine and Ethnobotany. 1991 Chromosome numbers of some alpine-subalpine taxa of Kashmir Himalaya. V Jee author 1983a text Herba Hungarica 1983a 22 23 31 Chromosome numbers reports LXXIX V Jee author 1983b text Taxon 1983b 32 Chromatin extrusion and cytomixis in pollen mother cells of Hordeum OP Kamra author 1960 text Hereditas 1960 46 592 600 Male meiosis, microsporogenesis and pollen grain study in Inula cuspidata C.B. Clarke (Asteraceae) D Kaur author 2010 text Cytologia 2010 75 363 368 Male meiotic studies in some plants of Polypetalae from Dalhousie hills (Himachal Pradesh) S Kaur author 2010 text Cytologia 2010 75 289 297 Comparison of common cytotypes of Andropogon gerardii (Andropogoneae, Poaceae). KH Keeler author 1999 text American Journal of Botany 1999 86 974 979 S Khatoon author 1993 Chromosome atlas of the angiosperms of Pakistan. 1993 M Koernicke author 1901 Uber ortsveranderung von Zellkarnern S B Niederhein Ges Natur-U Heilkunde Bonn A 1901 14 25 AK Koul author 1973 text Phyton 1973 15 57 66 Morphological and ploidy level variation of Centaruea phrygia agg. (Asteraceae) in the Czech Republic, Slovakia and Ukraine P Koutecký author 2007 text Folia Geobotanica 2007 42 77 102 A Kumar author 2010 Ethnobotany role in relation to medicinal plants in India 2010 Induced cytomictic variations through abiotic stresses in grasspea (Lathyrus sativus L.). G Kumar author 2008 text The Indian Journal of Genetics and Plant Breeding 2008 68 58 64 Cytology of Caltha palustris L. (Ranunculaceae) from cold regions of Western Himalayas P Kumar author 2008 text Cytologia 2008 73 137 143 Cytomixis induced meiotic abnormalities in pollen mother cells of Clematis flammula L. (Ranunculaceae) P Kumar author 2008a text Cytologia 2008a 73 381 385 Meiotic studies in species from the cold deserts of Lahaul-Spiti and adjoining areas (Northwest Himalayas) P Kumar author 2008b text Cytologia 2008b 73 463 470 P Kumar author 2010 Cytomixis and associated meiotic abnormalities affecting pollen fertility in Clematis orientalis 2010 54 181 184 Cytology of Ranunculus laetus Wall. ex Royle from cold desert regions and adjoining hills of North-west Himalayas. P Kumar author 2011 text Caryologia 2011 64 25 32 Impaired male meiosis due to irregular synapsis coupled with cytomixis in a new diploid cytotype of Dianthus angulatus Royle (Caryophyllaceae) from Indian cold deserts P Kumar author in press text Folia Geobotanica in press V Kumar author 1986 Chromosome atlas of flowering plants of the Indian sub-continent. 1986 Cytomixis in Lamiaceae. AK Kundu author 1988 text Cytologia 1988 53 469 474 Cytomixis impairs meiosis and influences reproductive success in Chlorophytum comosum (Thunb.) Jacq. – an additional strategy and possible implications SK Lattoo author 2006 text Journal of Biosciences 2006 31 629 637 Genetic diversity in diploid vs. tetraploid Rorippa amphibian (Brassicaceae) PC Luttikhuizen author 2007 text Molecular Ecology 2007 16 3544 3553 P Maheshwari author 1950 An introduction to the embryology of angiosperms. 1950 Cytomixis and its possible evolutionary role in a Kuwait population of Diplotaxis harra (Boraginaceae) GA Malallah author 2003 text Botanical Journal of the Linnaean Society 2003 143 169 175 Cytological study of some Himalayan Ranunculaceae PN Mehra author 1963 text Proceedings of the Indian Science Congress 1963 50B 453 454 Cytology of some West Himalayan Ranunculaceae. PN Mehra author 1972 text Cytologia 1972 37 281 296 EJ Mendes author 1951 A new interpretation for “Cytomixis”. Portugaliae Acta Biologica(SeriesA). 1951 3 211 288 Cytological and morphological variations in wild populations of Avena canariensis from the Canary Islands T Morikawa author 1996 text Genes & Genetic Systems 1996 71 15 21 Cytomixis in pollen mother cells of Ononis (Leguminosae). P Morisset author 1978 text Candian Journal of Genetics and Cytology 1978 20 383 388 Polyploidy in phenotypic space and invasion context: a morphometric study of Centaurea stoebe S.L. P Mráz author 2011 text International Journal of Plant Sciences 2011 172 386 402 An ultrastructural study of cytomixis in tobacco pollen mother cells SR Mursalimov author 2011 text Protoplasma 2011 Ploidy, geographical distribution and morphological differentiation of Parasenecio auriculata (Senecioneae, Asteraceae) in Japan M Nakagawa author 2006 text Journal of Plant Research 2006 119 51 61 Chromosomal and morphological studies of diploid and polyploid cytotypes of Stevia rebaudiana (Bertoni) Bertoni (Eupatorieae, Asteraceae) VM de Oliveira author 2004 text Genetics and Molecular Biology 2004 27 215 222 Floristic composition and biological spectrum of vegetation in alpine meadows of Kedarnath: Garhwal Himalaya. K Pharswan author 2010 text Nature and Science 2010 8 109 115 Cytotaxonomic studies in Indian Ranunculaceae. SC Roy author 1971 text The Nucleus 1971 14 132 143 SYA Semyarkhina author 1974 text ANBSSE Ser Biyal 1974 4 43 47 Cytomixis in Vigna glaberscens TTK (Wild). O Sen author 1988 text Cytologia 1988 53 437 440 Cytogenetic abnormalities caused by extremely low frequency electromagnetic fields in canola A Shabrangi author 2010 text Science Asia 2010 36 292 296 Annual Report of Cytogenetics Laboratory Department of Botany, University of Calcutta. AK Sharma author 1970 text Research Bulletin 1970 2 1 50 BD Sharma author 1993 Flora of India. Vol. 1. 1993 Meiotic studies of some Stipa (Poaceae) species and populations in Iran M Sheidai author 2005 text Cytologia 2005 70 23 31 H Singh author 2006 Flora of Chamba district (Himachal Pradesh). 2006 Cytomixis in some woody species. VK Singhal author 1985 text Biologica 1985 1 168 175 Spontaneous occurrence of meiotic spindle abnormalities in ‘Long Headed Poppy’ (Papaver dubium L.) from Indian cold regions VK Singhal author 2009 text Cytologia 2009 74 385 389 Impact of cytomixis on meiosis, pollen viability and pollen size in wild populations of Himalayan poppy (Meconopsis aculeata Royle) VK Singhal author 2008a text Journal of Biosciences 2008a 33 371 380 Cytomixis during microsporogenesis in the diploid and tetraploid cytotypes of Withania somnifera (L.) Dunal, 1852 (Solanaceae). VK Singhal author 2008b text Comparative Cytogenetics 2008b 2 85 92 Variable sized pollen grains due to impaired male meiosis in the cold desert plants of North West Himalayas (India). VK Singhal author JK Benjamin author 2010 text New York, USA, Nova Science Publishers 2010 101 126 Status of chromosomal diversity in the hardwood tree species of Punjab state. VK Singhal author 2007 text Journal of Cytology and Genetics 2007 8 67 83 Effect of cytomixis on the pollen size in ‘Seabuckthorn’ (Hippophae rhamnoides L., Elaeagnaceae) VK Singhal author 2008 text Cytologia 2008 73 167 172 Aberrant male meiosis, pollen sterility and variable sized pollen grains in Clematis montana Buch.-Ham. ex DC. from Dalhousie hills, Himachal Pradesh VK Singhal author 2010 text Cytologia 2010 75 31 36 Chromatin transfer during male meiosis resulted into heterogeneous sized pollen grains in Anemone rivularis Buch.- Ham. ex DC. from Indian cold deserts VK Singhal author 2009a text Cytologia 2009a 74 229 234 New detection of haploid chromosomes, pollen size and sterility in  Lychnis indica Benth.var. fimbriata Wall VK Singhal author 2009b text Chromosome Botany 2009b 4 53 56 Persistent occurrence of meiotic abnormalities in a new hexaploid cytotype of Thalictrum foetidum L. from Indian cold deserts VK Singhal author 2011 text Biologia 2011 66 458 464 Syncytes during male meiosis resulting into ’2n’ pollen grains formation in Lindelofia longiflora (Royle ex Benth.) Baill. var. falconeri (Cl.) Brand VK Singhal author in press text Journal of Systematics and Evolution in press Diploid and tetraploid cytotypes of Centaurea stoebe (Asteraceae) in central Europe: morphological differentiation and cytotype distribution patterns S Španiel author 2008 text Folia Geobotanica 2008 43 131 158 Revision of genus Ranunculus L. (Ranunculaceae) in India. SK Srivastava author 2010 text Taiwania 2010 55 273 314 Chromosome numbers and morphology in the Capsella bursa-pastoris complex (Brassicaceae) in Greece S Svensson author 1983 text Willdenowia 1983 13 267 276 Traditional use of medicinal plants among the tribal communities of Chhota Bhangal, Western Himalaya SKr Uniyal author 2006 text Journal of Ethnobiology and Ethnomedicine 2006 2 10.3897/compcytogen.v5i3.1359 https://compcytogen.pensoft.net/article/1727/ https://compcytogen.pensoft.net/article/1727/download/pdf/ In this study, we examined the chromosome number, detailed male meiosis, microsporogenesis, pollen fertility and morphological features and distribution of 2× and 4× cytotypes of Ranunculus hirtellus Royle, 1834. The majority of the populations scored now from cold regions of the northwest Himalayas showed tetraploid (n=16) meiotic chromosome count and one of the populations studied from the Manimahesh hills existed at diploid level (n=8). The individuals of diploid cytotype exhibited perfectly normal meiotic course resulting in 100% pollen fertility and pollen grains of uniform sizes. On the other hand, the plants of the tetraploid cytotype from all the populations in spite of showing normal bivalent formation and equal distribution to the opposite poles at anaphases showed various meiotic abnormalities. The most prominent among these meiotic abnormalities was the cytomixis which involved inter PMC (pollen mother cell) chromatin material transfer at different stages of meiosis-I. The phenomenon of cytomixis induced various meiotic abnormalities which include chromatin stickiness, pycnotic chromatin, laggards and chromatin bridges, out of plate bivalents at metaphase-I, disoriented chromatin material at anaphase/telophase and micronuclei. Consequently, these populations exhibited varying percentages of pollen sterility (24 - 77 %) and pollen grains of heterogeneous sizes. Analysis of various morphometric features including the stomata in 2× and 4× cytotypes showed that increase in ploidy level in the species is correlated with gigantism of vegetative and floral characters and the two cytotypes can be distinguished from each other on the basis of morphological characters. The distribution patterns of the 2× and 4× cytotypes now detected and 2×, 3×, 4× cytotypes detected earlier by workers from other regions of the Indian Himalayas have also been discussed. text/html en_US Pensoft Publishers Chromosome number cytotype cytomixis Lahaul-Spiti Manali hills Manimahesh hills meiotic abnormalities stomata. Male meiosis, morphometric analysis and distribution pattern of 2× and 4× cytotypes of Ranunculus hirtellus Royle, 1834 (Ranunculaceae) from the cold regions of northwest Himalayas (India) Research Article

10.3897/compcytogen.v5i3.1724 2011-08-24 compcytogen

University of Modena and Reggio Emilia, Italy author Monti, Valentina University of Modena and Reggio Emilia, Italy author Manicardi, Gian Carlo University of Modena and Reggio Emilia, Modena, Italy author Mandrioli, Mauro 2011-08-24 2011-08-24 2011 Comparative Cytogenetics 1993-078X 1993-0771 5 163 172 2011 Chromosome analysis and molecular characterization of highly repeated DNAs in the aphid Acyrthosiphon pisum (Aphididae, Hemiptera). D Bizzaro author 2000 text Genetica 2000 108 197 202 Chromosome numbers in the Aphididae and their taxonomic significance. RL Blackman author 1980 text Systematic Entomology 1980 5 7 25 Reproduction, cytogenetics and development. RL Blackman author AK Minsk author 1987 text Vol A. Elsevier, Amsterdam 1987 163 195 Behaviour of the X chromosomes during growth and maturation of parthenogenetic eggs of Amphorophora tuberculata (Homoptera, Aphididae) in relation to sex determination. RL Blackman author 1986 text Chromosoma 1986 94 59 64 Ribosomal DNA is frequently concentrated on only one X chromosome in permanently apomictic aphids, but this does not inhibit male determination. RL Blackman author 1996 text Chromosome Research 1996 4 314 320 Egg viability and sex determination in Megoura viciae (Homoptera, Aphididae). R Crema author 1979 text Entomologia Experimentalis & Applicata 1979 26 152 156 Lethal genes in a parthenogenetic strain of Megoura viciae. R Crema author 1981 text Bollettino Zoologico 1981 48 37 89 Methyl green is a substitute for distamycin A in the formation of distamycin A/DAPI C-bands. TA Donlon author 1983 text Human Genetics 1983 65 144 146 Strong biases in the transmission of sex chromosomes in the aphid Rhopalosiphum padi. A Frantz author 2005 text Genetical Research 2005 85 111 117 Phylogenetic distribution of TTAGG telomeric repeats in insects. R Frydrychová author 2004 text Genome 2004 47 163 178 Cytogenetic analysis on the holocentric chromosomes of the cabbage aphid Brevicoryne brassicae. S Giannini author 2003 text Caryologia 2003 56 143 147 Precocene causes male determination in the aphid Myzus persicae. DF Hales author 1983 text Journal of Insect Physiology 1983 29 819 823 The chromosomes of Scoutedenia lutea (Homoptrea, Aphididae, Greenidinae) with an account of meiosis in the male. D Hales author 1989 text Chromosoma 1989 98 295 300 Spatial organization of chromosomes in the salivary gland nuclei of Drosophila melanogaster. M Hochstrasser author 1986 text Journal of Cell Biology 1986 102 112 115 Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1 step-method. WM Howell author 1980 text Experientia 1980 36 1014 1015 Improved telomere detection using a telomere repeat probe (TTAGGG)n generated by PCR JW Ijdo author 1991 text Nucleic Acids Research 1991 19 Localization of tandemly repeated DNA sequences in beetle chromosomes by fluorescent in situ hybridization. C Juan author 1993 text Chromosome Research 1993 1 167 174 A preliminary note on the mitotic chromosome aberrations in embryos of whole-body X-irradiated parthenogenetic viviparous Aphis gossypii (Homoptera, Aphididae). AR Khuda-Buksh author 1981 text Chromosome Information Service 1981 30 4 5 Nucleolar organizing regions in aphid chromosomes. V Kuznetsova author 1991 text Tsitologya 1991 33 41 47 C-heterochromatin and NORs in X-chromosomes of aphid Dysaphis anthrisci Born. VG Kuznetsova author 1993 text Karyosystematics of Invertebrate Animals 1993 2 16 18 Structure, subnuclear distribution and nuclear matrix association of the mammalian telomeric complex. ME Luderus author 1996 text Journal of Cell Biology 1996 135 867 881 What genes and chromosomes say about the origin and evolution of insects and other arthropods. VA Lukhtanov author 2010 text Russian Journal of Genetics 2010 46 1115 1121 Cytogenetic characterization of telomeres in the holocentric chromosomes of the lepidopteran Mamestra brassicae. M Mandrioli author 2002 text Chromosome Research 2002 9 279 286 Composition and epigenetic markers of heterochromatin in the aphid Aphis nerii (Hemiptera: Aphididae). M Mandrioli author 2011 text Cytogenetics and Genome Research 2011 133 67 77 Molecular cytogenetic characterization of a highly repeated DNA sequence in the peach potato aphid Myzus persicae. M Mandrioli author 1999a text Chromosoma 1999a 108 436 442 The role of rDNA genes in X chromosomes association in the aphid Acyrthosiphon pisum. M Mandrioli author 1999b text Genome 1999b 42 381 386 Heterochromatin heterogeneity in the holocentric X chromatin of Megoura viciae (Homoptera, Aphididae). GC Manicardi author 1996 text Genome 1996 39 465 470 Chromosome banding in Aphids: G, C, AluI, and HaeIII banding patterns in Megoura viciae (Homoptera, Aphididae). GC Manicardi author 1991 text Genome 1991 34 661 665 Silver staining as a new banding technique to identify aphid chromosomes. GC Manicardi author 1998 text Chromosome Research 1998 6 55 57 Cytogenetic and molecular analysis of heterochromatic areas in the holocentric chromosomes of different aphid species GC Manicardi author RG Sobti author 2002 text Chromosome Structure and Function 2002 47 56 Karyotype of Psyllina (Homoptera). I. New data and check-list. A Maryańska-Nadachowska author 1992 text Folia biologica 1992 40 15 25 Chromosome banding in Cacopsylla mali (Schmidberger) and C. sorbi (Linnaeus) (Homoptera, Psyllidae) with polymorphic sex chromosomes. A Maryańska-Nadachowska author 2001 text Folia biologica 2001 49 157 161 Presence of a functional (TTAGG)n telomere-telomerase system in aphids V Monti author 2011 text Chromosome Research 2011 Evolutionary dynamics of rDNA clusters on chromosomes of moths and butterflies (Lepidoptera). P Nguyen author 2010 text Genetica 2010 138 343 354 Karyotype analysis of the Russian aphid Diuraphis noxia (Hemiptera: Aphididae) reveals a large X chromosome with rRNA and histone gene families. J Novotná author 2011 text Genetica 2011 139 281 289 Sex determination in Megoura viciae Bukton (Homoptera, Aphididae). E Orlando author 1974 text Monitore Zoologico Italiano 1974 8 61 70 SIR3 and SIR4 proteins are required for the positioning and integrity of yeast telomeres. F Palladino author 1993 text Cell 1993 75 543 555 Non-relationship between nucleolus and sex chromosome XY in Chelymorpha variubilis Boheman (Coleoptera: Chrysomelidae). A Postiglioni author 1988 text Genetica 1988 77 137 141 Chromosome ends: all the same under their caps. FE Pryde author 1997 text Current Opinion in Genetics & Development 1997 7 822 828 What is the (aphid) species? R Rakauskas author JM Nieto Nafria author 1998 text Universidad de Leon (Secretariado de Publicaciones), Lein 1998 451 455 Touch and go pairing in chromosomes. F Schrader author 1940 text Proceedings of the National Academy of Science USA 1940 26 634 636 Reverse fluorescent chromosome banding with chromomycin and DAPI. D Schweizer author 1976 text Chromosoma 1976 58 307 324 A simple technique for demonstrating centromeric heterochromatin. AT Sumner author 1972 text Experimental Cell Research 1972 75 304 306 Random loss of X chromosome at male determination in an aphid, Sitobion near fragariae, detected using an X-linked polymorphic microsatellite marker. ACC Wilson author 1997 text Genetical Research 1997 69 233 236 Cytogenetic of holokinetic chromosomes and inverted meiosis: keys to the evolutionary success of mites with generalization on eukaryotes. DL Wrensch author MA Houck author 1994 text Ecological and evolutionary analysis of life-history patterns. Chapman & Hall, New York, London 1994 282 343 10.3897/compcytogen.v5i3.1724 https://compcytogen.pensoft.net/article/1726/ https://compcytogen.pensoft.net/article/1726/download/pdf/ https://compcytogen.pensoft.net/article/1726/download/xml/ Cytogenetic and molecular investigations on the holocentric chromosomes of the aphid Macrosiphum euphorbiae (Thomas, 1878) have been carried out using silver staining and C-banding (followed by chromomycin A3 and DAPI staining) in order to improve our knowledge about the structure of aphid chromosomes. The C-banding pattern is peculiar since only the two X chromosomes and a single pair of autosomes presented heterochromatic bands. Silver staining and FISH with the 28S rDNA probe localized the rDNA genes on one telomere of each X chromosome that were also brightly fluorescent after chromomycin A3 staining of C-banded chromosomes, whereas all other heterochromatic bands were DAPI positive. Interestingly, a remarkable nucleolar organizing region (NOR) heteromorphism was present making the two X chromosomes easily distinguishable. Southern blotting and FISH assessed the presence of the (TTAGG)n repeat at the ends of all the M. euphorbiae chromosomes. Karyotype analysis showed that all males possessed the X chromosome with the larger amount of rDNA suggesting a non-Mendelian inheritance of the two X chromosomes. text/html en_US Pensoft Publishers aphid holocentric chromosomes telomeres heterochromatin NOR heteromorphism Cytogenetic and molecular analysis of the holocentric chromosomes of the potato aphid Macrosiphum euphorbiae (Thomas, 1878) Research Article

10.3897/compcytogen.v5i3.1185 2011-08-24 compcytogen

Royal holloway, University of London, United Kingdom author Angus, Robert https://orcid.org/https://orcid.org/0000-0002-3860-5617 University of London, United Kingdom author Tatton, A 2011-08-24 2011-08-24 2011 Comparative Cytogenetics 1993-078X 1993-0771 5 173 190 2011 Further karyosystematic investigations of the Stictotarsus griseostriatus (De Geer) group of sibling species (Coleoptera: Dytiscidae). Comparative Cytogenetics. RB Angus author 2008 text 2008 2 2 151 156 A third karyosystematic investigation of the Stictotarsus griseostriatus (De Geer) group of sibling species (Coleoptera: Dytiscidae). RB Angus author 2010a text Comparative Cytogenetics 2010a 4 1 13 20 Boreonectes gen. n., a new genus for the Stictotarsus griseostriatus (De Geer) group of sibling species (Coleoptera: Dytiscidae), with additional karyosystematic data on the group. RB Angus author 2010b text Comparative Cytogenetics 2010b 4 2 123 131 A revision of the Nebrioporus carinatus species complex (Coleoptera, Dytiscidae). RB Angus author 1992 text Nouvelle Revue d’Entomologie 1992 9 287 303 On Deronectes depressus, Fab., and elegans, Panzer WAF Balfour-Browne author 1919 text Annals and Magazine of Natural History 1919 3 393 308 The aquatic Coleoptera of the county of Angus, with a preliminary list for the county of Kincardine; and with further reference to the Deronectes depressus-elegans complex WAF Balfour-Browne author 1934 text Scottish Naturalist 1934 1934 15 25 A karyosystematic investigation of a group of sibling species related to Stictotarsus griseostriatus (De Geer) (Coleoptera: Dytiscidae). LA Dutton author 2007 text Comparative Cytogenetics 2007 1 3 16 Deronectes depressus F. und elegans Panz. Als Rassen einer Art. P Franck author 1935 text Entomologische Blätter für Biologie und Systematik der Käfer 1935 31 234 240 AN Nilsson author 2001 Dytiscidae (Coleoptera). World Catalogue of Insects3. 2001 AN Nilsson author 2011 Catalogue of Palaearctic Dytiscidae (Coleoptera). Internet version 2011–01–01 2011 A reclassification of the Deronectes-group of genera (Coleoptera: Dytiscidae) based on a phylogenetic study. AN Nilsson author 1992 text Entomologica Scandinavica 1992 23 275 288 Are Iberian endemics Iberian? A case-study using water beetles of the family Dytiscidae (Coleoptera). I Ribera author 2003 text Graellsia 2003 59 475 502 Chromosomal analysis of some Egyptian diving beetles (Coleoptera: Dytiscidae). R Saleh Ahmed author 2000 text Egyptian Journal of Biology 2000 2 76 84 A chromosomal investigation of five European species of Anacaena Thomson (Coleoptera: Hydrophilidae). FA Shaarawi author 1991 text Entomologica Scandinavica 1991 21 1990 415 426 DB Shirt author 1983 Studies on the Potamonectes depressus (Fabricius) complex of aquatic Coleoptera (Dytiscidae) 1983 AT Sumner author 2003 Chromosomes: organization and function. 2003 Revision in part of the genus Nebrioporus Regimbart, 1906, with emphasis on the Nebrioporus laeviventris-group (Coleoptera: Dytiscidae). M Toledo author 2009 text Zootaxa 2009 2040 1 111 10.3897/compcytogen.v5i3.1185 https://compcytogen.pensoft.net/article/1725/ https://compcytogen.pensoft.net/article/1725/download/pdf/ https://compcytogen.pensoft.net/article/1725/download/xml/ An account is given of the karyotypes of five species and one additional subspecies of Deronectes Sharp, 1882, three species of Stictotarsus Zimmermann, 1919, one species of Trichonectes Guignot, 1941, four species of Scarodytes Gozis, 1914 and 17 species of Nebrioporus Régimbart, 1906. Deronectes species are characterised by a neo-Xy system of sex chromosomes and autosome numbers ranging from 60 (D. ferrugineus Fery et Brancucci, 1987 and D. wewalkai Fery et Fresneda, 1988) through 48 (D. latus (Stephens, 1829), D. angusi Fery et Brancucci, 1990) to 28 (D. costipennis Brancucci, 1983, D. costipennis gignouxi Fery et Brancucci, 1989 and D. platynotus (Germar, 1834)). The three species of Stictotarsus, S. duodecimpustulatus (Fabricius, 1792), S. procerus (Aubé, 1838) and S. bertrandi (Legros, 1956), all belonging to the S. duodecimpustulatus group of species, have karyotypes comprising 54 autosomes and neo-Xy sex chromosomes. Trichonectes otini Guignot, 1941 has 48 autosomes and an X0 system of sex chromosomes, an arrangement shared with the 17 species of Nebrioporus Régimbart. The four Scarodytes species, S. halensis (Fabricius, 1787), S. nigriventris (Zimmermann, 1919), S. fuscitarsis (Aubé, 1838) and S. malickyi Wewalka, 1997, all have 54 autosomes and X0 sex chromosomes. The karyotypes of the various species are found to be distinctive and to support separation of these species from one another. In two cases (Nebrioporus martinii (Fairmaire, 1858) and N. sardus (Gemminger et Harold, 1868), and Scarodytes halensis and S. fuscitarsis) the karyotypes require the recognition of the taxa as full species, not subspecies. The implications of these data for the generic classification are considered. The data are found to be compatible with the DNA-based phylogeny proposed by Ribera (2003), where the enlarged Stictotarsus proposed by Nilsson and Angus (1992) is found to be unsatisfactory. text/html en_US Pensoft Publishers Chromosomes karyotypes Dytiscidae Deronectes Stictotarsus Scarodytes Trichonectes Nebrioporus species phylogeny. A karyosystematic analysis of some water beetles related to Deronectes Sharp (Coleoptera, Dytiscidae) Research Article

10.3897/compcytogen.v5i3.1730 2011-08-24 compcytogen

University of Florida, Gainesville, United States of America author Sourakov, Andrei Biodiversity Institute of Ontario, University of Guelph, Canada author Zakharov, Evgeny 2011-08-24 2011-08-24 2011 Comparative Cytogenetics 1993-078X 1993-0771 5 191 210 2011 Rapid morphological radiation and convergence among races of the butterfly Heliconius erato inferred from patterns of mitochondrial DNA evolution. AVZ Brower author 1994 text Proceedings of the National Academy of Sciences 1994 91 6491 6495 The status of Calisto hysius batesi (Lepidoptera, Satyridae) with the description of a new species of Calisto from Hispaniola. JC Correa author 1986 text Biological Sciences 1986 49 12 17 PJ De Vries author 1987 The Butterflies of Costa Rica and their Natural History. 1987 N Deer author 1949 The history of sugar. Vol. 1. 1949 DNA barcoding: a taxonomic point of view. RE DeWalt author 2011 text Journal of the North American Benthological Society 2011 30 1 174 181 Assembling DNA barcodes: analytical protocols JR deWaard author C Martin author 2008 text Methods in molecular biology: environmental genetics 2008 275 293 Confidence limits on phylogenies: An approach using the bootstrap. J Felsenstein author 1985 text Evolution 1985 39 783 791 Phylogeny of the Nymphalidae (Lepidoptera). AVL Freitas author 2004 text Systematic Biology 2004 53 363 383 Five new species of Calisto (Lepidoptera: Satyridae) from Hispaniola. F Gali author 1985 text Contributions in biology and geology, Milwaukee Public Museum 1985 63 1 16 Three new species and one new subspecies of the grannus complex of Hispaniolan Calisto (Lepidoptera: Satyridae). FL González author 1987 text Bulletin of the Allyn Museum 1987 108 1 17 DNA barcodes distinguish species of tropical Lepidoptera. M Hajibabaei author 2006 text Proceedings of the National Academy of Sciences 2006 103 968 971 Extinction and biogeography in the Caribbean: new evidence from a fossil riodinid butterfly in Dominican amber. JPW Hall author 2004 text Proceedings of the Royal Society of London B, 2004 271 1541 797 801 BioEdit: a user-friendly biological sequence alignment editor and analyses program for Windows 95/98/NT. TA Hall author 1999 text Nucleic Acids Symposium Series 1999 41 95 98 DNA barcodes for 1/1000 of the animal kingdom. PDN Hebert author 2010 text Biology Letters 2010 6 3 359 362 Insect pests of sugar cane. The leaf eating insects. Army worms, cutworms, leaf miners, grasshoppers and others. TE Holloway author 1933 text Facts About Sugar, New York, 1933 28 5 216 219 MRBAYES: Bayesian inference of phylogeny. JP Huelsenbeck author 2001 text Bioinformatics 2001 17 754 755 DH Huson author 2007 BMC Bioinformatics 2007 22 8(1) Three new species of Calisto from southwestern Haiti (Lepidoptera: Nymphalidae: Satyrinae). K Johnson author 1998 text Tropical Lepidoptera 1998 9 2 45 53 A new species of Calisto from Hispaniola with a review of the female genitalia of Hispaniolan congeners (Satyridae). K Johnson author 1987 text Journal of Research on the Lepidoptera 1987 25 2 73 82 Phylogeny of Agrodiaetus Hübner 1822 (Lepidoptera: Lycaenidae) inferred from mtDNA sof COI and COII and nuclear sequences of EF1-α: karyotype diversification and species radiation. NP Kandul author 2004 text Systematic Biology 2004 53 2 278 298 Early stages of the milkweed butterflies (Lepidoptera: Danainae). IJ Kitching author 1985 text Zoological Journal of Linnaean Society 1985 85 1 97 Effects of food plant on phenotypic plasticity in the tropical butterfly B. anynana. RE Kooi author 1996 text Entomologeia Experimentalis et Applicata 1996 80 149 151 . Nymphalidae, Satyrinae, Satyrini, Pronophilina. Checklist: Part 4A. Hesperioidea – Papilionoidea. G Lamas author JB Heppner author 2004 text Vol. 5A. Association for Tropical Lepidoptera, Scientific Publishers, Gainesville 2004 206 215 KJ Liebherr author 1988 (Ed) Zoogeography of Caribbean Insects. 1988 Analysis of an evolutionary species–area relationship. JB Losos author 2000 text Nature 2000 408 847 850 Adaptation, speciation, and convergence: a hierarchical analysis of adaptive radiation in Caribbean Anolis lizards. JB Losos author 2006 text Annals of the Missouri Botanical Garden 2006 93 1 24 33 DNA barcoding Central Asian butterflies: increasing geographical dimension does not significantly reduce the success of species identification. VA Lukhtanov author 2009 text Molecular Ecology Resources: 2009 9 1302 1310 The higher classification, phylogeny and zoogeography of the Satyridae (Lepidoptera). LD Miller author 1968 text Memoirs of American Entomological Society 1968 24 1 174 The biogeography of West Indian butterflies (Lepidoptera: Papilionidea, Hesperioidea): a vicariance model. LD Miller author CA Woods author 1989 text Gainesville: Sandhill Crane Press 1989 29 262 The systematics of Calisto (Lepidoptera: Satyridae) with remarks on the evolutionary and zoogeographic significance of the genus. EG Munroe author 1951 text Journal of the New York Entomological Society 1951 58 211 240 DL Murray author 2001 Systematic of euptychiine butterflies (Nymphalidae: Satyrinae: Euptychiina) based on larval morphology and DNA sequence data and the evolution of life history traits 2001 M Nei author 2000 Molecular Evolution and Phylogenetics. 2000 Revisiting the Insect Mitochondrial Molecular Clock: The Mid-Aegean Trench Calibration. A Papadopoulou author 2010 text Molecular Biology and Evolution 2010 27 7 1659 1672 The radiation of Satyrini butterflies (Nymphalidae: Satyrinae): a challenge for phylogenetic methods. C Peña author 2011 text Zoological Journal of the Linnaean Society 2011 161 64 87 Prehistorical climate change increased diversification of a group of butterflies. C Peña author 2008 text Biology Letters 2008 4 274 278 Higher level phylogeny of Satyrinae butterflies (Lepidoptera: Nymphalidae) based on DNA sequence data. C Peña author 2006 text Molecular Phylogenetics and Evolution 2006 40 29 49 A phylogenetic reassessment of Thisbe and Uraneis butterflies (Riodinidae, Nymphidiini). CM Penz author 2001 text Contributions in Science Los Angeles 2001 485 1 27 Evolution of the Gulf of Mexico and the Caribbean. JL Pindell author SK Donovan author 1994 text The University of the West Indies Publishers’ Association, Kingston 1994 13 39 NR Riley author 1975 A Field Guide to the Butterflies of the West Indies 1975 MRBAYES3: Bayesian phylogenetic inference under mixed models. F Ronquist author 2003 text Bioinformatics 2003 19 1572 1574 DS Smith author 1994 The Butterflies of the West Indies and South Florida. 1994 To keep out cane butterfly HEG Smyth author 1920 text Journal of Economic Entomology 1920 13 149 A Schwartz author 1989 Butterflies of Hispaniola. 1989 Five new species of Calisto (Satyridae) from Hispaniola. A Schwartz author 1984 text Bulletin of the Allyn Museum 1984 85 1 18 Systematics, Evolutionary Biology and Population Genetics of the Cercyonis pegala Group (Nymphalidae: Satyrinae). A Sourakov author 1995 text Holarctic Lepidoptera 1995 2 1 1 21 Notes on the genus Calisto, with descriptions of the immature stages (Part I) (Lepidoptera: Nymphalidae: Satyrinae) A Sourakov author 1996 text Tropical Lepidoptera 1996 7 1 91 112 A Sourakov author 1997 Morphology of immature stages in satyrinae (Lepidoptera:Nymphalidae) and its application to systematics 1997 Notes on the genus Calisto, with descriptions of the immature stages (Part II) (Lepidoptera: Nymphalidae: Satyrinae). A Sourakov author 2000 text Tropical Lepidoptera 2000 10 2 73 79 MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. K Tamura author 2007 text Molecular Biology and Evolution 2007 24 1596 1599 How common are dot-like distribution ranges? Taxonomical oversplitting in Western European Agrodiaetus (Lepidoptera, Lycaenidae) revealed by chromosomal and molecular markers. R Vila author 2010 text Biological Journal of the Linnean Society 2010 101 130 154 AL Viloria author 1998 Studies on the Systematics and Biogeography of Some Montane Satyrid butterflies (Lepidoptera) 1998 Does the DNA barcoding gap exist?- a case study in blue butterflies (Lepidoptera: Lycaenidae) M Wiemers author 2007 text Frontiers in Zoology 2007 4 Higher-level phylogeny of the Ithomiinae (Lepidoptera: Nymphalidae): classification, patterns of larval hostplant colonization and diversification. KR Willmott author 2006 text Cladistics 2006 22 297 368 Status of Calisto pulchella darlingtoni Clench (Lepidoptera: Satyridae). RW Wisor author 1985 text Florida Scientist 1985 48 1 7 13 (Ed) Woods CA author 1989 Biogeography of the West Indies: Past, Present, and Future. 1989 10.3897/compcytogen.v5i3.1730 https://compcytogen.pensoft.net/article/1724/ https://compcytogen.pensoft.net/article/1724/download/pdf/ The genus Calisto Hübner, 1823 is the only member of the diverse, global subfamily Satyrinae found in the West Indies, and by far the richest endemic Caribbean butterfly radiation. Calisto species occupy an extremely diverse array of habitats, suggestive of adaptive radiation on the scale of other classic examples such as the Galápagos or Darwin’s finches. However, a reliable species classification is a key requisite before further evolutionary or ecological research. An analysis of 111 DNA ‘barcodes’ (655 bp of the mitochondrial gene COI) from 29 putative Calisto species represented by 31 putative taxa was therefore conducted to elucidate taxonomic relationships among these often highly cryptic and confusing taxa. The sympatric, morphologically and ecologically similar taxa C. confusa Lathy, 1899 and C. confusa debarriera Clench, 1943 proved to be extremely divergent, and we therefore recognize Calisto debarriera stat. n. as a distinct species, with Calisto neiba Schwartz et Gali, 1984 as a junior synonym syn. n. Species status of certain allopatric, morphologically similar sister species has been confirmed: Calisto hysius (Godart, 1824) (including its subspecies C. hysius aleucosticha Correa et Schwartz, 1986, stat. n.), and its former subspecies C. batesi Michener, 1943 showed a high degree of divergence (above 6%) and should be considered separate species. Calisto lyceius Bates, 1935/C. crypta Gali, 1985/C. franciscoi Gali, 1985 complex, also showed a high degree of divergence (above 6%), confirming the species status of these taxa. In contrast, our data suggest that the Calisto grannus Bates, 1939 species complex (including Calisto grannus dilemma González, 1987, C. grannus amazona González, 1987, stat. n., C. grannus micrommata Schwartz et Gali, 1984, stat. n., C. grannus dystacta González, 1987, stat. n., C. grannus phoinix González, 1987, stat. n., C. grannus sommeri Schwartz et Gali, 1984, stat. n., and C. grannus micheneri Clench, 1944, stat. n.) should be treated as a single polytypic species, as genetic divergence among sampled populations representing these taxa is low (and stable morphological apomorphies are absent). A widely-distributed pest of sugar cane, Calisto pulchella Lathy, 1899 showed higher diversification among isolated populations (3.5%) than expected, hence supporting former separation of this species into two taxa (pulchella and darlingtoni Clench, 1943), of which the latter might prove to be a separate species rather than subspecies. The taxonomic revisions presented here result in Calisto now containing 34 species and 17 subspecies. Three species endemic to islands other than Hispaniola appear to be derived lineages of various Hispaniolan clades, indicating ancient dispersal events from Hispaniola to Puerto Rico, Cuba, and Jamaica. Overall, the degree of intrageneric and intraspecific divergence within Calisto suggests a long and continuous diversification period of 4–8 Myr. The maximum divergence within the genus (ca. 13.3%) is almost equivalent to the maximum divergence of Calisto from the distant pronophiline relative Auca Hayward, 1953 from the southern Andes (14.1%) and from the presumed closest relative Eretris Thieme, 1905 (14.4%), suggesting that the genus began to diversify soon after its split from its continental sister taxon. In general, this ‘barcode’ divergence corresponds to the high degree of morphological and ecological variation found among major lineages within the genus. text/html en_US Pensoft Publishers COI biogeography DNA barcoding islands intraspecific variation Lepidoptera Nymphalidae Satyrinae speciation taxonomy “Darwin’s butterflies”? DNA barcoding and the radiation of the endemic Caribbean butterfly genus Calisto (Lepidoptera, Nymphalidae, Satyrinae) Research Article

10.3897/compcytogen.v5i3.1435 2011-08-24 compcytogen

Moscow State University, Moscow, Russia author Gokhman, Vladimir https://orcid.org/0000-0001-9909-7559 Texas A&M University, United States of America author Johnston, Spencer The City College and The Graduate Center of The City University of New York, United States of America author Small, Chiyedza The City College and The Graduate Center of The City University of New York, United States of America author Rajwani, Roma Department of Entomology, Texas A&M University, United States of America author Hanrahan, Shawn The City College and The Graduate Center of The City University of New York, New York, United States of America author Govind, Shubha 2011-08-24 2011-08-24 2011 Comparative Cytogenetics 1993-078X 1993-0771 5 211 221 2011 Phylogeny of six African Leptopilina species (Hymenoptera: Cynipoidea, Figitidae), parasitoids of Drosophila, with description of three new species. R Allemand author 2002 text Annales de la Société entomologique de France 2002 38 319 332 An expansion of the genome size dataset for the insect order Hymenoptera, with a first test of parasitism and eusociality as possible constraints AM Ardila-Garcia author 2010 text Insect Molecular Biology 2010 19 337 346 Rapid SNP discovery and genetic mapping using sequenced RAD markers NA Baird author 2008 text PLoS One 2008 3 Analysis of Drosophila species genome size and satellite DNA content reveals significant differences among strains as well as between species G Bosco author 2007 text Genetics 2007 177 1277 1290 Identification and immuno-electron microscopy localization of p40, a protein component of immunosuppressive virus-like particles from Leptopilina heterotoma, a virulent parasitoid wasp of Drosophila H Chiu author 2006 text Journal of General Virology 2006 87 461 470 FlyBase: genomes by the dozen MA Crosby author 2007 text Nucleic Acids Research 2007 35 D486 D491 RH Crozier author 1975 Hymenoptera. 1975 Flybase author A Database of Drosophila Genes & Genomes, FB2011_04 Karyotype evolution in parasitic Hymenoptera VE Gokhman author 2004 text Entomological Review 2004 84 S41 S49 VE Gokhman author 2009 Karyotypes of Parasitic Hymenoptera 2009 Chromosomes of parasitic wasps of the genus Metaphycus (Hymenoptera: Chalcidoidea: Encyrtidae) VE Gokhman author 2010 text Comparative Cytogenetics 2010 4 21 25 Genome size diversity in the family Drosophilidae TR Gregory author 2008 text Heredity 2008 101 228 238 Widespread lateral gene transfer from intracellular bacteria to multicellular eukaryotes JCD Hotopp author 2007 text Science 2007 317 1753 1756 Modes of spontaneous chromosomal mutation and karyotype evolution in ants with reference to the minimum interaction hypothesis. HT Imai author 1988 text Japanese Journal of Genetics 1988 63 159 185 Tiny genomes and endoreduplication in Strepsiptera JS Johnston author 2004 text Insect Molecular Biology 2004 13 581 585 Evolution of host resistance and parasitoid counter-resistance AR Kraaijeveld author 2009 text Advances in Parasitology 2009 70 257 280 Hymenoptera: their biodiversity, and their impact on the diversity of other organisms. J LaSalle author J LaSalle author 1993 text CAB International, Wallingford 1993 1 26 Postembryonic hematopoiesis in Drosophila R Lanot author 2001 text Developmental Biology 2001 230 243 257 Virulence factors and strategies of Leptopilina spp.: selective responses in Drosophila hosts MJ Lee author 2009 text Advances in Parasitology 2009 70 123 145 Nomenclature for centromeric position on chromosomes. A Levan author 1964 text Hereditas 1964 52 201 220 Developmental analysis of Ganaspis xanthopoda, a larval parasitoid of Drosophila melanogaster. JP Melk author 1999 text Journal of Experimental Biology 1999 202 1885 1896 Role for sumoylation in systemic inflammation and immune homeostasis in Drosophila larvae I Paddibhatla author 2010 text PLoS Pathogens 2010 6 Cytology of Wolbachia-induced parthenogenesis in Leptopilina clavipes (Hymenoptera: Figitidae) BA Pannebakker author 2004 text Genome 2004 47 299 303 Morphological and molecular phylogenetics in the genus Leptopilina (Hymenoptera: Cynipoidea: Eucoilidae) M Schilthuizen author 1998 text Systematic Entomology 1998 23 253 264 Contrasting infection strategies in generalist and specialist wasp parasitoids of Drosophila melanogaster TA Schlenke author 2007 text PLoS Pathogens 2007 3 Phylogeny and classification of Hymenoptera. M Sharkey author 2007 text Zootaxa 2007 1668 521 548 Genetic analysis of contributions of dorsal group and JAK-Stat92E pathway genes to larval hemocyte concentration and the egg encapsulation response in Drosophila RP Sorrentino author 2004 text Genetics 2004 166 1343 1356 Inc StatSoft author 1995 STATISTICA for Windows. Computer Program Manual. 1995 The evolution of genome size in ants ND Tsutsui author 2008 text BMC Evolutionary Biology 2008 8 Drosophila-parasitoid communities as model systems for host-Wolbachia interactions F Vavre author 2009 text Advances in Parasitology 2009 70 299 331 10.3897/compcytogen.v5i3.1435 https://compcytogen.pensoft.net/article/1723/ https://compcytogen.pensoft.net/article/1723/download/pdf/ Drosophila melanogaster Meigen, 1830 has served as a model insect for over a century. Sequencing of the 11 additional Drosophila Fallen, 1823 species marks substantial progress in comparative genomics of this genus. By comparison, practically nothing is known about the genome size or genome sequences of parasitic wasps of Drosophila. Here, we present the first comparative analysis of genome size and karyotype structures of Drosophila parasitoids of the Leptopilina Förster, 1869 and Ganaspis Förster, 1869 species. The gametic genome size of Ganaspis xanthopoda (Ashmead, 1896) is larger than those of the three Leptopilina species studied. The genome sizes of all parasitic wasps studied here are also larger than those known for all Drosophila species. Surprisingly, genome sizes of these Drosophila parasitoids exceed the average value known for all previously studied Hymenoptera. The haploid chromosome number of both Leptopilina heterotoma (Thomson, 1862) and L. victoriae Nordlander, 1980 is ten. A chromosomal fusion appears to have produced a distinct karyotype for L. boulardi (Barbotin, Carton et Keiner-Pillault, 1979) (n = 9), whose genome size is smaller than that of wasps of the L. heterotoma clade. Like L. boulardi, the haploid chromosome number for G. xanthopoda is also nine. Our studies reveal a positive, but non linear, correlation between the genome size and total chromosome length in Drosophila parasitoids. These Drosophila parasitoids differ widely in their host range, and utilize different infection strategies to overcome host defense. Their comparative genomics, in relation to their exceptionally well-characterized hosts, will prove to be valuable for understanding the molecular basis of the host-parasite arms race and how such mechanisms shape the genetic structures of insect communities. text/html en_US Pensoft Publishers Drosophila parasitoid genome size karyotype evolution Genomic and karyotypic variation in Drosophila parasitoids (Hymenoptera, Cynipoidea, Figitidae) Research Article

10.3897/compcytogen.v5i3.1375 2011-08-24 compcytogen

Instituto de Biociências de Botucatu - UNESP, Botucatu, Brazil author Scacchetti, Priscilla Universidade Estadual Paulista (UNESP), Botucatu, Brazil author Pansonato-Alves, José Carlos Universidade Estadual Paulista (UNESP), Botukatu, Brazil author Utsunomia, Ricardo Universidade Estadual Paulista, Botucatu, Brazil author Oliveira, Claudio Universidade Estadual Paulista (UNESP), Botucatu, Brazil author Foresti, Fausto https://orcid.org/0000-0002-0862-0445 2011-08-24 2011-08-24 2011 Comparative Cytogenetics 1993-078X 1993-0771 5 223 235 2011 Chromosomal diversification of reef fishes from genus Centropyge (Perciformes, Pomacanthidae). PRAM Affonso author 2005 text Genetica 2005 123 227 233 Seven new species of the Neotropical electric fish Gymnotus (Teleostei, Gymnotiformes) with a redescription of G. carapo (Linnaeus). JS Albert author 2003 text Zootaxa 2003 287 1 54 Diversity and phylogeny of Neotropical electric fishes (Gymnotiformes). JS Albert author TH Bullock author 2005 text Springer New York 2005 360 403 Phylogenetic systematics and historical biogeography of the Neotropical electric fish Gymnotus (Teleostei: Gymnotidae). JS Albert author 2005 text Systematics and Biodiversity 2005 4 375 417 Karyotypic diversity between allopatric population of the group Hoplias malabaricus (Characiformes, Erythrinidae): evolutionary and biogeographic considerations. DR Blanco author 2010 text Neotropical Ichthyology 2010 8 361 368 Telomere shortening and tumor formation by mouse cells lacking telomerase RNA. MA Blasco author 1997 text Cell 1997 91 25 34 Evidence of chromosome fusion in Gymnotus sylvius Albert and Fernandes-Matioli, 1999 (Teleostei: Gymnotiformes) detected by telomeric probes and R-banding. FL Claro author 2010 text Caryologia 2010 63 134 141 Chromosome spreading of associated transposable elements and ribosomal DNA in the fish Erythrinus erythrinus. Implications for genome change and karyoevolution in fish. MB Cioffi author 2010 text BMC Evolutionary Biology 2010 10 271 280 Three new species from a diverse and sympatric assemblage of the electric fish Gymnotus (Ostariophysi: Gymnotidae) in the lowland Amazon Basin, with notes on ecology. WGR Crampton author 2005 text Copeia 2005 1 82 99 Molecular drive in multigene families: How biological novelties arise, spread and are assimilated. GA Dover author 1986 text Trends in Genetics 1986 2 159 165 Chromosomal changes and adaptation of Cichlid fishes during evolution. E Feldberg author AL Val author 2003 text New Delhi and New York. Science Publishers 2003 285 308 Extensive nucleolus organizer region polymorphism in Gymnotus carapo (Gymnotoidei, Gymnotidae). FMC Fernandes-Matioli author 1997 text Cytogenetics Cell Genetics 1997 78 236 239 High intraspecific karyological conservation in four species of Gymnotus (Pisces: Gymnotiformes) from Southeastern Brazilian basins. FMC Fernandes-Matioli author 1998 text Caryologia 1998 3 221 234 A molecular phylogenetic analysis in Gymnotus species (Pisces: Gymnotiformes) with inferences on chromosome evolution. FMC Fernandes-Matioli author 2001 text Caryologia 2001 54 23 30 Polymorphic nature of nucleolus organizer regions on fishes. F Foresti author 1981 text Cytogenetics Cell Genetics 1981 31 137 144 A method for chromosome preparations from large specimens of fishes using in vitro short treatment with colchicine. F Foresti author 1993 text Experientia 1993 49 810 813 R Froese author 2011 FishBase. World Wide Web electronic publication 2011 Chromomycin A3 stains nucleolus organizer regions of fish chromosomes. JR Gold author 1986 text Copeia 1986 1 226 231 Controlled silver staining of nucleolus organizer regions with a protective colloidal developer: A 1-step method. WM Howell author 1980 text Experientia 1980 36 1014 1015 Cytogenetic Analysis of Three Sympatric Gymnotus Species (Teleostei: Gymnotidae) from the Fundo Stream, MG, Brazil. MCV Lacerda author 2007 text Cytologia 2007 72 89 93 Nomenclature for centromeric position on chromosomes. A Levan author 1964 text Hereditas 1964 52 201 220 Electric fishes of the continental waters of America. Biblioteca de la Academia de Ciencias Fisicas, Matematicas y Naturales. F Mago-Leccia author 1994 text Caracas 1994 29 1 206 Cytogenetic analysis of three sympatric Gymnotus (Gymnotiformes, Gymnotidae) species verifies invasive species in the Upper Paraná River basin, Brazil. VP Margarido author 2007 text Journal of Fish Biology 2007 70 155 164 Chromosomal localization of 5s rDNA genes in Leporinus fish (Anostomidae, haraciformes). C Martins author 1999 text Chromosome Research 1999 7 363 367 Two rDNA arrays in Neotropical fish species: is it a general rule for fishes? C Martins author 2001 text Genetica 2001 111 439 446 Gymnotus capanema, a new species of electric knife fish (Gymnotidae-Gymnotiformes)PageBreak from eastern Amazonia, with comments on an unusual karyotype SSR Milhomem author in press text Journal of Fish Biology in press Chromosomal evidence for a cryptic species in the Gymnotus carapo species-complex (Gymnotiformes, Gymnotidae) SSR Milhomem author 2008 text BMC Evolutionary Biology 2008 9 75 Multiple rearrangements in cryptic species of electric knifefish, Gymnotus carapo (Gymnotidae, Gymnotiformes) revealed by chromosome painting CY Nagamachi author 2010 text BMC Genetics 2010 11 Karyotypic conservatism in samples of Characidium cf. zebra (Teleostei, Characiformes, Crenuchidae): Physical mapping of ribosomal genes and natural triploidy. JC Pansonato-Alves author 2011b text Genetics and Molecular Biology 2011b 34 208 213 Interspecific chromosomal divergences in the genus Characidium (Teleostei, Characiformes, Crenuchidae). JC Pansonato-Alves author 2010 text Neotropical Ichthyology 2010 8 77 86 Chromosomal diversification in populations of Characidium cf. gomesi (Teleostei, Crenuchidae). JC Pansonato-Alves author 2011a text Journal of Fish Biology 2011a 78 183 194 Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. D Pinkel author 1986 text Procedures of the National Academy of Sciences 1986 83 2934 2938 Reverse fluorescent chromosome banding with chromomycin and DAPI. D Schweizer author 1976 text Chromosoma 1976 58 307 324 An X1X1X2X2/X1X2Y multiple sex chromosome system in a new species of the genus Gymnotus (Pisces, Gymnotiformes). EB Silva author 2005 text Environmental Biology of Fishes 2005 73 293 297 A simple technique for demonstrating centromeric heterochromatin. AT Sumner author 1972 text Experimental Cell Research 1972 75 304 306 Variation in satellite DNA profiles-causes and effects. D Ugarkovic author 2002 text The EMBO Journal 2002 21 5955 5959 Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics TJ White author 1990 text PCR Protocols: A Guide to Methods and Applications 1990 312 315 10.3897/compcytogen.v5i3.1375 https://compcytogen.pensoft.net/article/1722/ https://compcytogen.pensoft.net/article/1722/download/pdf/ Conventional (Giemsa, C-Banding, Ag-NORs, CMA3) and molecular (5S rDNA, 18S rDNA, telomeric sequences) cytogenetic studies were carried out in specimens of ten distinct fish populations of the genus Gymnotus (G. sylvius Albert and Fernandes-Matioli, 1999, G. inaequilabiatus Valenciennes, 1839, G. pantherinus Steindachner, 1908, and G. cf. carapo Linnaeus, 1758) from different Brazilian hydrographic basins. G. sylvius presented a diploid number of 40 chromosomes (22m+12sm+6st), G. pantherinus presented 52 chromosomes (32m+18sm+2st), while G. inaequilabiatus (42m+10sm+2a) and G. cf. carapo (38m+12sm+4st) presented 54 chromosomes. The C-banding technique revealed centromeric marks in all chromosomes of all species. Besides that, conspicuous blocks of heterochromatin were found interstitially on the chromosomes of G. inaequilabiatus, G. cf. carapo, and G. pantherinus. All four species showed single nucleolus organizing regions confirmed by results obtained through Ag-NORs and FISH experiments using 18S rDNA probes, which showed the NORs localized on the first chromosome pair in G. inaequilabiatus, G. cf. carapo, and G. pantherinus, and on pair 2 in G. sylvius. CMA3 staining revealed additional unrelated NORs marks in G. sylvius and G. pantherinus. The 5S rDNA probes revealed signals on one pair in G. sylvius and two pairs in G. pantherinus; G. inaequilabiatus had about seventeen pairs marked, and G. cf. carapo had about fifteen pairs marked. It is considered that the high amount of heterochromatin identified in the chromosomes of G. inaequilabiatus and G. cf. carapo could have facilitated the dispersion of 5S rDNA in these species. Interstitial signals were detected on the first metacentric pair of G. sylvius by telomeric probes (TTAGGG)n indicating the possible occurrence of chromosomal fusions in this species. The present study reveals valuable cytotaxonomic markers for this group and allows a more precise evaluation of the processes involved in the karyotype differentiation and the interrelationships among different species of the genus Gymnotus. text/html en_US Pensoft Publishers FISH rDNA cytogenetic heterochromatin chromosomal rearrangements. Karyotypic diversity in four species of the genus Gymnotus Linnaeus, 1758 (Teleostei, Gymnotiformes, Gymnotidae): physical mapping of ribosomal genes and telomeric sequences Research Article

10.3897/compcytogen.v5i3.1235 2011-08-24 compcytogen

Departamento de Biologia Geral, CCB, Universidade Estadual de Londrina, Londrina, Brazil author Pacheco, R. B. Universidade Estadual de Maringá, Parana, Brazil author Rosa, Renata https://orcid.org/0000-0003-4258-7244 Universidade Estadual de Londrina, Londrina, Brazil author Giuliano-Caetano, L. Departamento de Biologia Celular e Genética, Universidade Estadual de Maringá, Maringá, Brazil author Júlio Jr., H. F. Departamento de Biologia Geral, CCB, Universidade Estadual de Londrina, Londrina, Brazil author Dias, Ana Lúcia 2011-08-24 2011-08-24 2011 Comparative Cytogenetics 1993-078X 1993-0771 5 237 246 2011 E Abelini author 2007 Análise citogenética em três espécies do gênero Astyanax (Pisces, Characiformes) 2007 Conservation of the 5S-bearing chromosome pair and co-localization with major rDNA clusters in five species of Astyanax (Pisces, Characidae) LF Almeida–Toledo author 2002 text Cytogenetic and Genome Research 2002 97 3–4 229 233 Nature and distribution of constitutive heterochromatin in fishes, genus Hypostomus (Loricariidae) RF Artoni author 1999 text Genetica 1999 106 3 209 214 LAC Bertollo author 1978 text Brazilian Journal of Genetics 1978 1 2 103 120 Chromosome R-banding pattern and conservation of a marker chromosome in four species, genus Astyanax (Characidae, Tetragonopterinae). MFZ Daniel-Silva author 2001 text Caryologia 2001 54 3 209 215 Cytogenetic and comparative morphology of two allopatric populations of Astyanax altiparanae Garutti et Britski, 2000 (Teleostei: Characidae) from upper rio Paraná basin MS Domingues author 2007 text Neotropical Ichthyolology 2007 5 1 37 44 Cytogenetic studies in two populations of Astyanax altiparanae (Pisces, Characiformes) CA Fernandes author 2004 text Hereditas 2004 3 141 332 Mapping of the 18S and 5S ribosomal RNA genes in Astyanax altiparanae Garutti et Britski, 2000 (Teleostei, Characidae) from the upper Paraná river basin, Brazil CA Fernandes author 2006a text Genetics and Molecular Biology 2006a 29 1 1 5 Chromosomal location of 5S and 18S rRNA genes in three sympatric cytotypes of Astyanax scabripinnis (Characiformes, Characidae) from the Ivaí river basin, state of Paraná, Brazil. CA Fernandes author 2006b text Caryologia (Firenze) 2006b 59 3 253 259 Comparative cytogenetics among populations of Astyanax altiparanae (Characiformes, Characidae, Incertae sedis) M Ferreira Neto author 2009 text Genetics and Molecular Biology 2009 32 4 792 796 Descrição de uma espécie nova de Astyanax (Teleostei: Characidae) da bacia do alto rio Paraná e considerações sobre as demais espécies do gênero na bacia. Comunicações do Museu de Ciência e Tecnologia. V Garutti author 2000 text Série Zoologia 2000 13 65 88 Mapping of the 18S and 5S ribosomal RNA genes in the fish Prochilodus argenteus Agassiz, 1829 (Characiformes, Prochilodontidae) T Hatanaka author 2004 text Genetica (The Hague) 2004 122 3 239 244 Controlled silver-staining of nucleolus organizer regions with the protective coloidal developer: a 1-step method WM Howell author 1980 text Experientia 1980 36 8 1014 1015 Nomenclature for centromeric position of chromosomes A Levan author 1964 text Hereditas 1964 52 201 220 Genera Incertae sedis in Characidae FCT Lima author RE Reis author 2003 text Check list of the freshwater fishes of South and Central America 2003 106 109 Accentuated polymorphism of heterochromatin and nucleolar organizer regions in Astyanax scabripinnis (Pisces, Characidae): tools for understanding karyotypic evolution M Mantovani author 2000 text Genetica 2000 109 3 161 168 Conserved 5S and variable 45S rDNA chromosomal localization revealed by FISH in Astyanax scabripinnis (Pisces, Characidae) M Mantovani author 2005 text Genetica 2005 3 123 216 Chromosomal localization of 5S rDNA genes in Leporinus fish (Anostomidae, Characiformes) C Martins author 1999 text Chromosome Research 1999 7 5 363 367 Two 5S rDNA arrays in Neotropical fish species: is it a general rule for fishes C Martins author 2001 text Genetica 2001 111 1–3 439 446 Cytogenetics considerations on the genus Astyanax (Pisces, Characidae), I Karyotypic variability. S Morelli author 1983 text Caryologia 1983 36 3 235 244 RB Pacheco author 2001 Estudos citogenéticos em diferentes populações de Astyanax altiparanae (Pisces, Tetragonopterinae) 2001 Occurrence of cytotypes and multiple NORs in an Astyanax altiparanae population (Pisces, Tetragonopterinae). RB Pacheco author 2001 text Chromosome Science 2001 5 2 109 114 HH Paganelli author 1990 Diversidade cromossômica no gênero Astyanax, com especial referência a Astyanax bimaculatus. (Linnaeus, 1758). Considerações citotaxonômicas e evolutivas 1990 Chromosome polymorphism in Astyanax fasciatus (Teleostei, Characidae).1. Karyotype analysis, Ag-NORs and mapping of the 18S and 5S ribosomal genes in sympatric karyotypes and their possible hybrid forms R Pazza author 2006 text Cytogenetic Genome Research 2006 112 3–4 313 319 Physical mapping of the 18S and 5S ribosomal genes in nine Characidae species (Teleostei, Characiformes) WAM Peres author 2008 text Genetics and Molecular Biology 2008 31 1 222 226 Cytogenetic analysis of Astyanax laticeps (Cope, 1894) (Ostariophysi: Characidae) from the laguna dos Patos system R Rosa author 2009 text Neotropical Ichthyology 2009 7 4 601 605 Reverse fluorescent chromosome banding with chromomycin and DAPI D Schweizer author 1976 text Chromosoma 1976 4 58 324 Non-radom distribution of the GC-rich heterochromatin and nuclear rDNA sites on Astyanax scabripinnis chromosomes. IL Souza author 2001 text Cytologia 2001 66 1 85 91 Cytogenetic Characterization of the Large South American Siluriform Fish Species Zungaro zungaro (Pisces, Pimelodidae). AC Swarça author 2001 text Chromosome Science 2001 5 1 51 55 10.3897/compcytogen.v5i3.1235 https://compcytogen.pensoft.net/article/1721/ https://compcytogen.pensoft.net/article/1721/download/pdf/ Two populations of Astyanax altiparanae (Garutti et Britski, 2000) of the Água dos Patos stream/SP and lake Igapó/PR were analyzed. All individuals showed 2n = 50, however, different karyotypic formulae were observed. The population of the Água dos Patos stream showed 8m +24sm+6st+12a (NF=88) and the population of lake Igapó, 8m+28sm+4st+10a (NF=90). Nucleolus organizing regions (AgNORs) were observed in the terminal position on the short and long arm of different chromosomes of both populations, showing a variation from 3 to 4 chromosomes. Fluorescent in situ hybridization (FISH) using 18S rDNA probes revealed only one pair of chromosomes with fluorescent signals in the terminal site on the short arm in the Igapó lake population, while the population of Água dos Patos stream showed 4 fluorescence terminal signals, characterizing a system of simple and multiple NORs, respectively. 5S rDNA fluorescent signals were detected in the interstitial position of a pair of chromosomes in the two studied populations. Some AgNOR sites revealed to be GC-rich when stained with Chromomycin A3 (CMA3), however, AT positive regions were not observed. The data obtained show that, despite the conservation of the diploid number and location of 5S DNAr, differences in both the distribution of 18S rDNA and karyotypic formula among the populations were found, thus corroborating the existing data on chromosome variability in Astyanax altiparanae that can be significant for cytotaxonomy in this group. text/html en_US Pensoft Publishers Incertae sedis FISH karyotypic formulae NORs Cytogenetic comparison between two allopatric populations of Astyanax altiparanae Garutti & Britski, 2000 (Teleostei, Characidae), with emphasis on the localization of 18S and 5S rDNA Research Article

10.3897/compcytogen.v5i3.1429 2011-08-24 compcytogen

University of Rome "Sapienza", Rome, Italy author Gornung, Ekaterina https://orcid.org/0000-0003-3963-3318 Fundação Oswaldo Cruz, IOC, Rio de Janeiro, Brazil author Bezerra, Alexandra M. R. https://orcid.org/0000-0002-7972-5535 University of Rome La Sapienza, Roma, Italy author Castiglia, Riccardo https://orcid.org/0000-0002-9011-302X 2011-08-24 2011-08-24 2011 Comparative Cytogenetics 1993-078X 1993-0771 5 247 257 2011 Characterization of the satellite DNA Msat-160 from species of Terricola (Microtus) and Arvicola (Rodentia, Arvicolinae). MJ Acosta author 2010 text Genetica 2010 138 1085 1098 Human intrachromosomal telomeric-like repeats: sequence organization and mechanisms of origin. CM Azzalin author 2001 text Chromosoma 2001 110 75 82 Heterogeneous heterochromatin and size variation in the sex chromosomes of Microtus cabrerae M Burgos author 1988 text Cytogenetics and Cell Genetics 1988 47 75 79 Systematics of the Microtus savii complex (Rodentia, Cricetidae) via mitochondrial DNA analyses: paraphyly and pattern of sex chromosome evolution. R Castiglia author 2008 text Molecular Phylogenetics and Evolution 2008 3 1157 64 Are ribosomal RNA gene clusters rearrangement hotspots? A case study in the genus Mus (Rodentia, Muridae) B Cazaux author 2011 text BMC Evolutionary Biloogy 2011 11 On subspecific taxonomy of Microtus savii (Rodentia, Arvicolidae). L Contoli author 2003 text Hystrix - Italian Journal of Mammalogy 2003 14 107 111 Microtus savii (de Sélys Longchamps, 1838). L Contoli author G Amori author 2008 text 44 Mammalia II Erinaceomorpha, Soricomorpha, Lagomorpha, Rodentia. Bologna 2008 538 540 Microtus brachycercus (von Lehmann, 1961) L Contoli author G Amori author 2008 text Fauna d’Italia 44. Mammalia II Erinaceomorpha, Soricomorpha, Lagomorpha, Rodentia 2008 535 538 Caratteri generali dei Microtus (Terricola) del “gruppo savii” L Contoli author G Amori author 2008 text Fauna d’Italia 44. Mammalia II Erinaceomorpha, Soricomorpha, Lagomorpha, Rodentia 2008 525 535 Purposes Council of Europe: European Convention for the Protection of Vertebrate Animals used for Experimental and other Scientific author 1986 Strasbourg: Council of Europe. 1986 Finely orchestrated movements: evolution of the ribosomal RNA genes. TH Eickbush author 2007 text Genetics 2007 175 477 485 Karyology of the Savi pine vole, Microtus savii (De Sélys-Longchamps, 1838) (Rodentia, Arvicolidae): G-, C-, DA/DAPI-, and AluI-bands. L Galleni author 1992 text Cytogenetics and Cell Genetics 1992 59 290 292 Taxonomy of Microtus savii (Rodentia, Arvicolidae) in Italy: cytogenetic and hybridization data. Journal of. L Galleni author 1994 text Mammalogy 1994 75 1040 1044 Biogeographical and karyological data of Microtus savii group (Rodentia Arvicolidae) in Italy. L Galleni author 1998 text Bonner Zoologische Beiträge 1998 47 277 282 Comparative cytogenetic study of two sister species of Iberian ground voles, Microtus(Terricola) duodecimcostatus and Microtus (T.) lusitanicus (Rodentia, Cricetidae). E Gornung author 2011 text Cytogenetic and Genome Research 2011 132 144 150 Genomic dispersion of 28S rDNA during karyotypic evolution in the ant genus Myrmecia (Formicidae). H Hirai author 1996 text Chromosoma 1996 105 190 196 Molecular phylogeny of the specious vole genus Microtus (Arvicolinae, Rodentia) inferred from mitochondrial DNA sequences. M Jaarola author 2004 text Molecular Phylogenetics and Evolution 2004 33 647 663 Chromosomal evolution of Arvicolinae (Cricetidae, Rodentia). III. Karyotype relationships of ten Microtus species. N Lemskaya author 2010 text Chromosome Research 2010 18 459 471 In situ hybridization to human metaphase chromosomes using DIG- or biotinlabeled DNA probes and detection with fluorochrome conjugates P Lichter author 1992 text Nonradioactive in situ Hybridization (Application manual) 1992 25 27 X chromosome painting in Microtus: Origin and evolution of the giant sex chromosomes. JA Marchal author 2004 text Chromosome Research 2004 12 767 776 Karyotypic relationships of the Tatra vole (Microtus tatricus). N Martínková author 2004 text Folia Zoolica 2004 53 279 284 Comparative analysis of chromosomes in Microtus transcaspicus and Microtus subarvalis (Arvicolidae, Rodentia): high-resolution G-banding and localisation of NORs. NA Mazurok author 1996 text Hereditas 1996 124 243 250 Comparative chromosome and mitochondrial DNA analyses and phylogenetic relationships within common voles (Microtus, Arvicolidae). NA Mazurok author 2001 text Chromosome Research 2001 9 107 120 Pattern of X–Y chromosome pairing in the Taiwan vole, Microtus kikuchii. K Mekada author 2001 text Genome 2001 44 27 31 Heterochromatin study and geographical distribution of Microtus species (Rodentia, Arvicolinae) from Greece. GP Mitsainas author 2009 text Mammalian Biology 2009 75 261 26 C-banding analyses and the evolution of heterochromatin among arvicolid rodents. WS Modi author 1987 text Journal of Mammalogy 1987 68 704 714 Superfamily Muroidea. GG Musser author DE Wilson author 2005 text Baltimore 2005 894 1531 Insertion of telomeric repeats at intrachromosomal break sites during primate evolution. SG Nergadze author 2004 text Genome Research 2004 14 1704 1710 Comparative mapping of X chromosomes in vole species of the genus Microtus. TB Nesterova author 1998 text Chromosome Research 1998 6 41 48 Reorganization of the X chromosome in voles of the genus Microtus. NB Rubtsov author 2002 text Cytogenetic and Genome Research 2002 99 323 329 A simple technique for demonstrating centromeric heterochromatin. AT Sumner author 1972 text Experimental Cell Research 1972 75 304 306 Karyotypic variability and systematics of the Arvicolini (Rodentia) 2 Correlation pattern of chromosomal numbers IV Zagorodnyuk author 1992 text Vestnik Zoologii 1992 1992 36 45 Karyotypes of European mammals II. J Zima author 1984 text Acta Scientiarum Naturalium Academiae Scientiarum Bohemoslovace Brno 1984 18 3 62 10.3897/compcytogen.v5i3.1429 https://compcytogen.pensoft.net/article/1720/ https://compcytogen.pensoft.net/article/1720/download/pdf/ The Microtus (Terricola) savii s. l. complex is a group of five species/subspecies of the Italian pine voles, which diverged at different times either with or without chromosomal differentiation. The evidence of chromosomal diversification has so far concerned the shape of the sex chromosomes, especially the X chromosome. Three taxa of the group, M. savii savii, M. s. nebrodensis, and M. s. tolfetanus have identical karyotypes with metacentric X chromosomes. The X chromosomes of M. brachycercus and M. b. niethammericus are, respectively, subtelocentric and acrocentric in shape. The M. savii complex has been long an object of conventional karyological studies, but comparative molecular cytogenetic data were completely missing. Therefore, we conducted a comparative chromosomal mapping of rRNA genes (rDNA) and telomeric repeats in three of the five taxa of the group: Microtus s. savii, M. s. nebrodensis, and M. b. niethammericus, each of which belongs to a distinct mitochondrial clade. The survey revealed that differentiation of the clades was accompanied by remarkable changes with regard to the number and locations of the rDNA sites. Thus, M. s. savii and M. s. nebrodensis have especially high numbers of rDNA sites, which are located in the centromeric regions of, correspondingly, 18 and 13 chromosome pairs, whereas M. b. niethammericus shows variable (8–10) and heteromorphic rDNA sites on both centromeric and telomeric regions. Interstitial telomeric sites (ITS), which are believed to indicate possible breakpoints of recurring chromosomal rearrangements, are present on the largest biarmed chromosomes and on the metacentric X chromosomes in M. s. savii and M. s. nebrodensis. These preliminary results are discussed in the context of recent advances in phylogeny of the group, as well as the rDNA genomic organization and X chromosome rearrangements in the genus Microtus. text/html en_US Pensoft Publishers Arvicolinae chromosomal evolution sex chromosomes interstitial telomeric sequences (ITS) rDNA NORs Comparative chromosome mapping of the rRNA genes and telomeric repeats in three Italian pine voles of the Microtus savii s.l. complex (Rodentia, Cricetidae) Research Article

10.3897/compcytogen.v5i4.1834 2011-11-09 compcytogen

Universidade Federal do Rio Grande do Norte, Brazil author Galvão, Tatiana Universidade Federal de São Carlos, Brazil author Bertollo, Luiz Universidade Federal do Rio Grande do Norte, Natal - RN, Brazil author Molina, Wagner https://orcid.org/0000-0002-6695-0952 2011-11-09 2011-11-09 2011 Comparative Cytogenetics 1993-078X 1993-0771 5 259 275 2011 PRAM Affonso author 2000 Caracterização citogenética de peixes de recifes de corais da família Pomacanthidae (Perciformes). Master thesis, Universidade Federal de São Carlos, São Carlos, SP, Brasil. 2000 Cytogenetic analysis of coral reef fishes from Brazil (families Pomacanthidae and Chaetodontidae). PRAM Affonso author 2001 text Cytologia 2001 66 379 384 Evolutionary aspects of gobioid fishes based upon a phylogenetic analysis of mitochondrial cytochrome b genes. A Akihito author 2000 text Gene 2000 259 5 15 Phylogenetic relationships of the north-eastern Atlantic and Mediterranean blenniids. 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Oviedo, 1994, Oviedo, 66. 1994 Karyotypes of five species of Blennioidea (Pisces, Perciformes). R Vitturi author 1986 text Caryologia 1986 39 273 279 Multiple chromosome polymorphism in the gobiid fish Gobius niger jozo L. 1758 (Pisces, Gobiidae). R Vitturi author 1989 text Cytologia 1989 54 231 235 Molecular phylogeny of gobioid fishes (Perciformes: Gobioidei) based on mitochondrial 12S rRNA sequences. HY Wang author 2001 text Molecular Phylogenetics and Evolution 2001 20 309 408 Karyology of the Indo-Pacific Parioglossus raoi (Herre) (Teleostei: Gobioidei) from Fiji. CJ Webb author 1986 text Australian Journal of Marine and Freshwater Research 1986 37 347 351 Genetic evidence against panmixia in European eel. T Wirth author 2001 text Nature 2001 409 1037 1040 An observation of the chromosomes in the embryonic cells of a goby, Chaenogobius urotaenia (Hilgendorf). J Yamada author 1967 text Bulletin of the Faculty of Fisheries Hokkaido University 1967 18 183 187 10.3897/compcytogen.v5i4.1834 https://compcytogen.pensoft.net/article/1735/ https://compcytogen.pensoft.net/article/1735/download/pdf/ A remarkable degree of chromosomal conservatism (2n=48, FN=48) has been identified in several families of Perciformes. However, some families exhibit greater karyotypic diversity, although there is still scant information on the Atlantic species. In addition to a review of karyotypic data available for representatives of the suborders Blennioidei and Gobioidei, we have performed chromosomal analyses on Atlantic species of the families Blenniidae, Ophioblennius trinitatis Miranda-Ribeiro, 1919 (2n=46; FN=64) and Scartella cristata (Linnaeus, 1758) (2n=48; FN=50), Labrisomidae, Labrisomus nuchipinnis (Quoy & Gaimard, 1824) (2n=48; FN=50) and Gobiidae, Bathygobius soporator (Valenciennes, 1837) (2n=48; FN=56). Besides variations in chromosome number and karyotype formulas, Ag-NOR sites, albeit unique, were located in different positions and/or chromosome pairs for the species analyzed. On the other hand, the heterochromatic pattern was more conservative, distributed predominantly in the centromeric/pericentromeric regions of the four species. Data already available for Gobiidae, Blenniidae and Labrisomidae show greater intra- and interspecific karyotypic diversification when compared to other groups of Perciformes, where higher uniformity is found for various chromosome characteristics. Evolutionary dynamism displayed by these two families is likely associated with population fractionation resulting from unique biological characteristics, such as lower mobility and/or specific environmental requirements. text/html en_US Pensoft Publishers Chromosomal evolution marine fish Bleniidae Gobiidae Labrisomidae Chromosomal complements of some Atlantic Blennioidei and Gobioidei species (Perciformes) Research Article

10.3897/compcytogen.v5i4.1796 2011-11-09 compcytogen

North Eastern Hill University, India author Hynniewta, Marlykynti National Bureau of Plant Genetic Resources, India author Malik, Surendra Kumar North Eastern Hill University, Shillong, India author Rama Rao, Satyawada 2011-11-09 2011-11-09 2011 Comparative Cytogenetics 1993-078X 1993-0771 5 277 287 2011 Observacoes citologicas em Citrus: I. Numero de cromosomios de algumas especies e variedades. O Bacchi author 1940 text Journol de Agronomia 1940 3 249 258 A numerical taxonomic study of affinity relationships in cultivated Citrus and its close relatives. HC Barrett author 1976 text Systematic Botany 1976 1 105 136 Colchicine induced polyploidy in Citrus. HC Barrett author 1974 text Botanical Gazette 1974 135 29 41 The evolution of CMA bands in Citrus and related genera. AE Barros e Silva author 2010 text Chromosome Research 2010 18 503 514 HVL Bathla author 2001 Agricultural Research Data Book 2001. IASRI and ICAR, New Delhi, 113 p. 2001 Classifications of Citrus fruits of Assam. SC Bhattacharya author 1956 text ICAR Science Monograph 1956 20 1 110 Chromosome morphology and terminology. E Battaglia author 1955 text Caryologia 1955 6 179 187 JW Cameron author 1969 Characters of new populations of Citrus polyploids, and the relation between tetraploidy in the pollen parent and hybrid tetraploid progeny. Proceedings of the first International Citrus Symposium1, June 1969 Riverside, California, USA, 199–205. 1969 Phylogenic relationships within the genus Citrus (Rutaceae) and related genera as revealed by RFLP and RAPD analysis. CT Federici author 1998 text Theoretical Applied Genetics 1998 96 812 822 Tetraploidy in Citrus. HB Frost author 1925a text Proceedings of National Academy Science of USA 1925a 11 535 537 The chromosomes of Citrus. HB Frost author 1925b text Journal Washington Academy of Science 1925b 15 1 3 Chromosome number and secondary constriction variation in 51 accessions of a Citrus germplasm bank. M Guerra author 1997 text Brazilian Journal of Genetics 1997 20 489 496 IA Khan author 2007 Citrus Genetics, Breeding and Biotechnology. CAB International 160 pp. 2007 Chromosome numbers in the subfamily Aurantioideae with special reference to the genus Citrus. CA Krug author 1943 text Botanical Gazzetts 1943 104 602 611 Triploid varieties of Citrus. CA Krug author 1943 text Journal of Heredity 1943 34 277 283 WK Lapin author 1937 Investigations on polyploidy in Citrus. U.S.S.R. All-Union Science Research Institute for Humid Sub-tropic Works 1(4): 1–68. [In Russian with English summary] 1937 Nomenclature for centromeric position on chromosome. A Levan author 1964 text Hereditas 1964 52 201 220 Polycarpy, polyspory and polyploidy in Citrus and Citrus relatives. AE Longley author 1925 text Journal Washington Academy of Science 1925 15 347 351 AI Luss author 1935 Citrus introduction and selection in the U.S.S.R., Soviet Sub-tropics 11: 17–22 [Cited in Plant Breeding Abstract 6: entry 1372] 1935 Collection and characterization of Citrus indica Tanaka and C. macroptera Montr.: wild endangered species of northeastern India. SK Malik author 2006 text Genetic Resources and Crop Evolution 2006 53 1485 1493 J McPhee author 1967 Oranges. New York, 76 pp. 1967 Citrus phylogeney and genetic origin of important species as investigated by molecular markers. E Nicolosi author 2000 text Theoretical Applied Genetics 2000 100 1155 1166 P Osbeck author 1757 Dagbok ofwer en Ostindisk Resa, p.98. Lor Ludv Grefing, Stockholm. 1757 I Oiyama author 1991 Use of pollen from a somatic hybrid between Citrus and Poncirus in the production of triploids. Horticulural Science26:1082. 1991 Studies on polyploid breeding in Citrus. (3) Triploid progenies from small seeds of diploid sweet orange cultivars. I Oiyama author 1980 text Journal of the Japanese Society of Horticultural Sciences 1980 3 52 53 Production of Colchicine-induced autotetraploid plants through micrografting in monoembryonic citrus cultivars. I Oiyama author 1986 text Japanese Journal of Breeding 1986 36 371 376 A critical review and a new proposal of karyotype asymmetry indices. B Paszko author 2006 text Plant Systematics and Evolution, 2006 258 39 48 On the history and origin of Citrus. RW Scora author 1975 text Bulletin of the Torrey Botanical Club 1975 102 369 375 Genetic resources of Citrus of north-eastern India and their potential use. BD Sharma author 2004 text Genetic Resources and Crop Evolution 2004 51 411 418 HP Singh author 1993 Genetic Resources of Citrus: In: Advances in Horticulture, Vol.1 (Eds) Malhotra Publishing House, New Delhi. 164 pp. 1993 WT Swingle author 1967 The botany of Citrus and its wild relatives. In: Reuther W, Batchelor LD, Webber HJ (Eds) The Citrus Industry, University of California press, Berkeley, 190–340. 1967 Taxonomie de la Citriculture tropicale. T Tanaka author 1930 text Revue de Botanique Applique 1930 10 114 119 Fundamental discussion of Citrus classification. T Tanaka author 1977 text Studia Citrologica 1977 14 1 6 RC Upadhayay author 1998 Crop gene pools in the North-East Indian Himalayan and threats. In: Pratap T, Sthapit B (Eds) Managing Agrobiodiversity-Farmers Changing Perspective and Institutional Responses in the Hindu Kush-Himalayan Region ICIMOD&IPGRI, Kathmandu, Nepal, 167–173. 1998 Seed development in relation to ploidy of zygotic embryo and endosperm in polyembryonic Citrus. A Wakana author 1981 text Proceedings International Society of Citriculture 1981 1 35 39 CMA banding patterns of Chromosomes in major Citrus species. M Yamamoto author 2007 text Journal of JapaneseSociety of Horticultural Science 2007 76 1 36 40 10.3897/compcytogen.v5i4.1796 https://compcytogen.pensoft.net/article/1734/ https://compcytogen.pensoft.net/article/1734/download/pdf/ https://compcytogen.pensoft.net/article/1734/download/xml/ Ten Citrus (Linnaeus, 1753) species of North-East India have been karyo-morphologically analysed. All studied species had 2n=18 chromosomes without any evidence of numerical variation. All the chromosomes were found to be of metacentric and sub-metacentric in all the species; the morphology of the chromosomes showing size difference only. Symmetrical karyotype which does not have much difference in the ratio of longest to shortest chromosome in all the species was observed. Three species, C. grandis (Osbeck, 1757), C. reticulata (Blanco, 1837) and C. medica (Linnaeus, 1753) are identified as true basic species from asymmetry studies of karyotypes as they reflect on the primitive nature of their genomes. C. indica (Tanaka, 1937) occupies a special taxonomic position within the genus Citrus as a progenitor for other cultivated species text/html en_US Pensoft Publishers Citrus karyotype genetic variability asymmetry index Karyological studies in ten species of Citrus (Linnaeus, 1753) (Rutaceae) of North-East India Research Article

10.3897/compcytogen.v5i4.1757 2011-11-09 compcytogen

Instituto Federal de Educação Ciencia e Tecnologia de Mato Grosso, Cuiaba, Brazil author Mariotto, Sandra Universidade Federal do Mato Grosso, Brazil author Centofante, Liano Universidade Estadual de Ponta Grossa, Ponta Grossa, Brazil author Vicari, Marcelo https://orcid.org/0000-0003-3913-9889 Universidade Estadual de Ponta Grossa. Depto. Biologia Estrutural, Molecular e Genética. Ponta Grossa, Ponta Grossa, Brazil author Artoni, Roberto Universidade Federal de São Carlos, Brazil author Moreira-Filho, Orlando 2011-11-09 2011-11-09 2011 Comparative Cytogenetics 1993-078X 1993-0771 5 289 300 2011 Karyotype variability in eight species of the subfamilies Loricariinae and Ancistrinae (Teleostei, Siluriformes, Loricariidae). AL Alves author 2003 text Caryologia 2003 56 57 63 Phylogenetic relationships of the suckermouth armoured catfishes (Loricariidae) with emphasis on the Hypostominae and the Ancistrinae. JW Armbruster author 2004 text Zoological Journal of the Linnean Society 2004 141 1 80 Trends in the karyotype evolution of Loricariidae fish (Siluriformes). RF Artoni author 2001 text Hereditas 2001 134 201 210 Chromossomal markers and phylogenetic inferences among species of Parodontidae. E Bellafronte author 2011 text Reviews in Fish Biology and Fisheries 2011 21 559 570 Citotaxonomic considerations on Hoplias lacerdae (Pisces, Erythrinidae). LAC Bertollo author 1978 text Brazilian Journal of Genetics 1978 1 103 120 V Bueno author 2011 Trends in chromosome evolution in the genus Hypostomus Lacépède, 1803 (Osteichthyes, Loricariidae): a new perspective about the correlation between diploid number and chromosomes types. Reviews in Fish Biology and Fisheries. 2011 Interindividual size heteromorphism of NOR and Chromosomal location of 5S rDNA genes in Iheringichthys labrosus (Pisces, Pimelodidae). RA Carvalho author 2007 text Brazilian Archives of Biology and Technology 2007 50 141 146 Cytogenetic characterization and description of an XX/XY1Y2 sex chromosome system in catfish Harttia carvalhoi (Siluriformes, Loricariidae). L Centofante author 2006 text Cytogenetic and Genome Research 2006 112 320 324 Chromosome painting in farm, pet and wild animal species. BP Chowdhary author 2001 text Methods in Cell Science 2001 23 37 55 Molecular phylogeny of the Neoplecostominae and Hypoptopomatinae (Siluriformes: Loricariidae) using multiple genes. CA Cramer author 2011 text Molecular Phylogenetics and Evolution 2011 59 43 52 Karyotype characterization and ZZ/ZW sex chromosomes heteromorphism in two species of the catfish genus Ancistrus Kner, 1854 (Siluriformes:Loricariidae) from the Amazon basin. RR de Oliveira author 2007 text Neotropical Ichthyology 2007 5 301 306 Karyotype description of three species of Loricariidae (Siluriformes) and ocurrence of the ZZ/ZW sexual system in Hemiancistrus spilomma Cardoso & Lucinda, 2003. RR de Oliveira author 2006 text Neotropical Ichthyology 2006 4 93 97 Chromosome diversity in neotropical fishes: NOR studies. PM Galetti author 1998 text Italian Journal of Zoology 1998 65 53 56 C Garcia author 2008 Localization of ribosomal genes in three Pimelodus species (Siluriformes, Pimelodidae) of the São Francisco River: 5S genes as species markers and conservation of the 18S rDNA sites. Genetics and Molecular Biology 31(suppl): 261–264. 2008 Mapping of the 18S and 5S ribosomal RNA genes in the fish Prochilodus argenteus Agassiz 1829 (Characiformes, Prochilodontidae). T Hatanaka author 2004 text Genetica 2004 122 239 244 Controlled silver-staining of nucleolus organizer regions whit a protective coloidae developer: a l-step method. WM Howell author 1980 text Experientia 1980 36 1014 1015 Classification and catalogue of the mailed Loricariidae (Pisces, Siluriformes). IJH Isbrücker author 1980 text Verslagen en Technische Gegevens 1980 22 1 181 Chromosomal location and distribution of As51 satellite DNA in five species of the genus Astyanax (Teleostei, Characidae, Incertae sedis). DLZ Kantek author 2009 text Journal of Fish Biology 2009 75 408 421 Heterochromatin characterization of four fish species of the family Loricariidae (Siluriformes). KF Kavalco author 2004 text Hereditas 2004 141 237 242 Repeated genes in eukaryotes. EO Long author 1980 text Annual Review of Biochemistry 1980 49 727 764 TC Machado author 2011 Chromosomal painting and ZW sex chromosomes differentiation in Characidium (Characiformes, Crenuchidae). BMC Genetics 12: 65. 2011 Occurrence of sexual chromosome, of the type ZZ/ZW, in Ancistrus cf. dubius (Loricariidae, Ancistrinae) of the Paraguay River Basin, Mato Grosso, Brazil. S Mariotto author 2004 text Caryologia 2004 57 327 331 Chromosome polymorphism in Ancistrus cuiabae Knaack, 1999 (Siluriformes, Loricariidae, Ancistrini). S Mariotto author 2009 text Neotropical Ichthyology 2009 7 595 600 Ancistrus cf. dubius (Siluriformes, Ancistrinae), a complex of species. 1. Chromosomal characterization of four populations and occurrence of sex chromosomes of the type XX/XY, in the Pantanal Basin of Mato Grosso, Brazil. S Mariotto author 2006 text Caryologia 2006 59 299 304 Cytogenetical analyses in two endemic fish species from the São Francisco river basin: Conorhynchus conirostris and Lophiosilurus alexandri (Siluriformes). MBA Marques author 2008 text Genetics and Molecular Biology 2008 31 215 221 Chromosomal localization of 5S rDNA genes in Leporinus elongatus fish (Anostomidae, Characiformes). C Martins author 1999 text Chromosome Research 1999 7 363 367 Chromosomal polymorphism in Steindachneridion melanodermatum Garavello, 2005 (Siluriformes, Pimelodidae): a reappraisal the existence of sex chromosome system in the species. DA Matoso author 2011 text Reviews in Fish Biology and Fisheries 2011 21 497 508 Description of karyotype in Hypostomus regani (Ihering, 1905) (Teleostei, Loricariidae) from the Piumhi river in Brazil with comments on karyotype variation found in Hypostomus. EOM Mendes Neto author 2011 text Comparative Cytogenetics 2011 5 133 142 Different cytotypes in fishes of the genus Hypostomus Lacépède, 1803, (Siluriformes: Loricariidae) from Xingu river (Amazon region, Brazil). SSR Milhomem author 2010 text Comparative Cytogenetics 2010 4 11 12 Karyotype variability in neotropical catfishes of the family Pimelodidae (Teleostei: Siluriformes). A Moraes Neto author 2011 text Neotropical Ichthyology 2011 9 97 105 Chromossomal mapping and nucleotide sequence of two tandem repeats of Atlantic Salmo 5S rDNA. AM Pendás author 1994 text Cytogeneticand Cell Genetics 1994 67 31 36 Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. D Pinkel author 1986 text Proceedings of the National Academy of Sciences USA 1986 83 2934 2938 Delturinae, a new loricariid catfish subfamily (Teleostei, Siluriformes), with revisions of Delturus and Hemipsilichthys. RE Reis author 2006 text Zoological Journal of the Linnean Society 2006 147 277 299 Natural triploidy in Rhamdia quelen identified by cytogenetic monitoring in Iguaçu basin, southern Brazil. M Silva author 2011 text Environmental Biology of Fishes 2011 91 361 366 Cytogenetic analysis and chromosomal characteristics of the polymorphic 18S rDNA in the fish Prochilodus lineatus (Characiformes, Prochilodontidae). MR Vicari author 2006 text Genetics and Molecular Biology 2006 29 621 625 Co-localization of repetitive DNAs and silencing of major rDNA genes. A case report in the fish, Astyanax janeiroensis. MR Vicari author 2008 text Cytogenetic and Genome Research 2008 122 67 72 Satellite DNA in Neotropical fishes: Methods, applications and perspectives. MR Vicari author 2010 text Journal of Fish Biology 2010 76 1094 1116 K Ziemniczak author 2011 Estudo citogenético em espécies de Loricariidae (Pisces, Siluriformes) das nascentes dos rios Ribeira e Tibagi, Ponta Prossa – PR. 2011 10.3897/compcytogen.v5i4.1757 https://compcytogen.pensoft.net/article/1733/ https://compcytogen.pensoft.net/article/1733/download/pdf/ https://compcytogen.pensoft.net/article/1733/download/xml/ Populations of seven Ancistrus species were analyzed from streams and rivers of three hydrographic Brazilian basins. All populations showed different diploid numbers (2n), fundamental numbers (FNs), and karyotypes. Some representatives of Loricariidae have 2n = 54 chromosomes, which is very likely an ancestral cytotaxonomic characteristic, but many other representatives show extensive karyotype diversification. In the Ancistrus species studied, extensive karyotypic differentiation, which is generally associated with chromosome number reduction and rearrangement of the ribosomal RNA gene (rDNA) sites, was verified. Chromosomal locations of 18S and 5S rDNA were jointly detected using fluorescence in situ hybridization (FISH). In all the Ancistrus species analyzed, 18S rDNA sites were detected only on one chromosome pair, though this differed among species. 5S rDNA was located on 1–3 chromosome pairs either separately or in synteny with 18S rDNA in four of the seven species/populations. Hence the karyotype differentiation in Ancistrus species could be associated with a morphological speciation process, suggesting that chromosome fusions, inversions, deletions, duplications, and heterochromatination could contribute to the karyotype evolution of these neotropical armored catfishes. text/html en_US Pensoft Publishers karyotype evolution Robertsonian rearrangement Ag-NORs 18S rDNA 5S rDNA Chromosomal diversification in ribosomal DNA sites in Ancistrus Kner, 1854 (Loricariidae, Ancistrini) from three hydrographic basins of Mato Grosso, Brazil Research Article

10.3897/compcytogen.v5i4.1650 2011-11-09 compcytogen

Universidade Estadual de Londrina/Paraná/Brazil, Brazil author Sampaio, Tatiane Ramos Instituto nacional da Amazônia - INPA, Brazil author Gravena, Waleska Universidade Estadual de Londrina/Paraná/Brazil, Brazil author Gouveia, Juceli Gonzales Universidade Estadual de Londrina, Londrina, Brazil author Giuliano-Caetano, L. Departamento de Biologia Geral, CCB, Universidade Estadual de Londrina, Londrina, Brazil author Dias, Ana Lúcia 2011-11-09 2011-11-09 2011 Comparative Cytogenetics 1993-078X 1993-0771 5 301 313 2011 Cytotaxonomic considerations on Hoplias lacerdae (Pisces, Erythrinidae). LAC Bertollo author 1978 text Brazilian Journal of Genetics 1978 1 2 103 120 Study on karyotype and occurrence of B chromosomes in two enedemic species of the genus Pimelodus (Siluriformes, Pimelodidae) from the River Iguaçu. LA Borin author 2004 text Hereditas 2004 140 201 209 Comparative cytogenetics studies of Curimatidae (Pisces, Characiformes) from the middle Paraná River (Argentina). MS Brassesco author 2004 text Genetics and Molecular Research 2004 3 2 293 301 B-chromosome evolution. JPM Camacho author 2000 text Philosophical Transactions of the Royal Society of London B: Biological Sciences 2000 355 1394 163 178 Cytogenetic analysis of three species of the families Characidae and Curimatidae (Teleostei, Characiformes) from the Acre River. ML Carvalho author 2001 text Chromosome Science 2001 5 91 96 B chromosomes: an update about their occurrence in freshwater Neotropical fishes (Teleostei). RA Carvalho author 2008 text Journal of Fish Biology 2008 72 1907 1932 Cytogenetic analyses of two Curimatidae species (Pisces; Characiformes) from the Paranapanema and Tietê Rivers. LV De Rosa author 2007 text Brazilian Journal of Biology 2007 67 2 333 338 Identification and description of distinct B chromosomes in Cyphocharax modestus (Characiformes, Curimatidae). LV De Rosa-Santos author 2008 text Genetics and Molecular Biology 2008 31 1 265 269 Synapsis in supernumerary chromosomes of Prochilodus lineatus (Teleostei: Prochilodontidae). AL Dias author 1998 text Caryology 1998 51 2 105 113 Karyotype evolution in Curimatidae (Teleostei, Characiformes) of the Amazon region. I. Studies on the genera Curimata, Psectrogaster, Steindachnerina and Curimatella. E Feldberg author 1992 text Brazilian Journal of Genetics 1992 15 2 369 383 Karyotype evolution in Curimatidae (teleostei, Characiformes) from the Amazon region. II. Centric fissions in the genus Potamorhina. E Feldberg author 1993 text Genome 1993 36 372 376 B chromosomes in Amazon cichlid species. E Feldberg author 2004 text Cytogenetics and Genome Research 2004 106 195 198 A cytogenetic survey of the fish fauna from Argentina. AS Fenocchio author 2003 text Caryologia 2003 2 197 204 W Gravena author 2007 Occurrence of B chromosomes in Cyphocharax modestus (Fernández-Yépez., 1948) and Steindachnerina insculpta (Fernández-Yépez, 1948) (Characiformes, Curimatidae) from the Tibagi River basin (Paraná State, Brazil). Brazilian Journal of Biology 67(4 Suppl): 905–908. 2007 RN Jones author 1982 B chromosomes. 1982 Rapid chromosome preparations from solid tissues of fishes. AD Kligerman author 1977 text Journal of the Fisheries Research Board of Canada 1977 34 266 269 Nomenclature for centromeric position on chromosomes. A Levan author 1964 text Hereditas 1964 52 201 220 Occurrence of a B chromosome in Cyphocharax modesta (Pisces, Curimatidae). C Martins author 1996 text Cytobios 1996 85 247 253 VPO Moraes author 2009 B Chromosomes in Four Different Populations of Rhamdia quelen (Siluriformes, Heptapteridae): A Comparative Study of Frequency and Distribution. Folia Biologica (Kraków) 57: 3–4: 165–169. 2009 B chromosomes in the fish Astyanax scabripinnis (Characidae, Tetragonopterinae): overview in natural populations. O Moreira-Filho author 2004 text Cytogenetics and Genome Research 2004 106 2–4 230 234 Cytogenetic analysis of four curimatids from the Paraguay Basin, Brasil (Pisces: Characiformes, Curimatidae). MC Navarrete author 1997 text Cytologia 1997 62 241 247 Occurrence of supernumerary microchromosomes in Steindachnerina insculpta (Pisces, Characiformes, Curimatidae). C Oliveira author 1993 text Cytobios 1993 76 183 186 Possible origins of B chromosomes in Rinelocaria pentamaculata (Loricariidae, Silurifomes) from the Paraná River basin. FE Porto author 2010 text Genetics and Molecular Research 2010 9 3 1654 1659 Karyotypic analysys in two species of fishes of the family Curimatidae: AgNO3, CMA3 and FISH with 18S probe. R Teribele author 2008 text Caryologia 2008 61 3 211 215 Natural triploidy and chromosome B in the fish Curimata modesta (Curimatidae, Characiformes). PC Venere author 1985 text Brazilian Journal of Genetics 1985 8 681 687 Chromosome evolution and phylogenetic relationships of some neotropical Characiformes of the family Curimatidae. PC Venere author 1989 text Brazilian Journal of Genetics 1989 12 17 25 Multiple longitudinal bands in fish chromosomes: comparison of structural G-banding and replication R bands among curimatids. PC Venere author 1995 text Cytobios 1995 84 71 78 New cases of supernumerary chromosomes in Characiform fishes. PC Venere author 1999 text Genetics and Molecular Biology 1999 22 3 345 349 Recent chromosome diversification in the evolutionary radiation of the freshwater fish family Curimatidae (Characiformes). PC Venere author 2008 text Journal of Fish Biology 2008 72 1976 1989 10.3897/compcytogen.v5i4.1650 https://compcytogen.pensoft.net/article/1732/ https://compcytogen.pensoft.net/article/1732/download/pdf/ https://compcytogen.pensoft.net/article/1732/download/xml/ In the present work, six curimatid species were analyzed: Cyphocharax voga (Hensel, 1870), C. spilotus (Vari, 1987), C. saladensis (Meinken, 1933), C. modestus (Fernández-Yépez, 1948), Steindachnerina biornata (Braga & Azpelicueta, 1987) and S. insculpta (Fernández-Yépez, 1948) collected from two hydrographic basins. All samples presented 2n=54 meta-submetacentric (m-sm) chromosomes and FN equal to 108, and 1 or 2 B microchromosomes in the mitotic and meiotic cells of the six sampled populations showing inter-and intraindividual variation. The analysis of the meiotic cells in C. saladensis, C. spilotus, and C. voga showed a modal number of 54 chromosomes in the spermatogonial metaphases and 27 bivalents in the pachytene, diplotene, diakinesis and in metaphase I stages, and 27 chromosomes in metaphase II; in C. modestus, S. biornata, and S. insculpta, spermatogonial metaphases with 54 chromosomes and pachytene and metaphase I with 27 bivalents were observed. The B microchromosome was observed as univalent in the spermatogonial metaphase of C. spilotus, in the pachytene stage in the other species, with the exception of C. saladensis, and S. biornata in metaphase I. New occurrences of the B microchromosome in C. voga, C. saladensis and S. biornata were observed, confirming that the presence of this type of chromosome is a striking characteristic of this group of fish. text/html en_US Pensoft Publishers B microchromosome meiosis curimatids. B microchromosomes in the family Curimatidae (Characiformes): mitotic and meiotic behavior Research Article

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Private, Australia author Porter, David The University of Melbourne, Parkville, Australia author Martin, Jon 2011-11-09 2011-11-09 2011 Comparative Cytogenetics 1993-078X 1993-0771 5 315 328 2011 Origin and evolution of animal hybrid species. L Bullini author 1994 text Trends in Ecology and Evolution 1994 9 422 426 CD Darlington author 1962 The handling of chromosomes. 4th Edn. London, 262 pp. 1962 Plain water as a rinsing agent preferable to sulfurous acid after Feulgen reaction. P Demalsy author 1967 text Stain Technology 1967 42 133 136 JA Downes author 1962 What is an Arctic insect? Canadian Entomologist 94: 143–162. 1962 Phylogenetic utility of five genes for dipteran phylogeny: A test case in the Chironomidae leads to generic synonymies. T Ekrem author 2010 text Molecular Phylogenetics and Evolution 2010 57 561 571 Birth and death of genes linked to chromosomal inversion. Y Furuta author 2011 text Proceedings of the National Academy of Sciences 2011 108 1501 1506 Polyploidy in animals. TR Gregory author TR Gregory author 2005 text San Diego 2005 427 517 Some aspects of natural selection in arrhenotokous populations. DL Hartl author 1971 text American Zoologist 1971 11 309 325 The salivary gland chromosomes of Chironomus oppositus Walker (Diptera: Nematocera). Australian. J Martin author 1969 text Journal of Zoology 1969 17 473 486 Polytene chromosomes and phylogenetic relationships of Chironomus atrella (Diptera: Chironomidae) in North America. J Martin author 2006 text Genome 2006 49 1384 1392 The polytene chromosomes and their significance to the systematics of the family Chironomidae, Diptera. P Michailova author 1989 text Acta Zoologica Fennica 1989 186 1 107 Sex ratios of some high Arctic Chironomidae (Diptera). DR Oliver author 1972 text Canadian Entomologist 1972 104 1413 1417 Oogenesis and chromosomal heterozygosity in the thelytokous midge, Lundstroemia parthenogenetica (Diptera, Chironomidae). DL Porter author 1971 text Chromosoma 1971 32 332 342 Die Chromosomen parthenogenetischer Mücken. H Scholl author 1956 text Naturwissenschaften 1956 43 91 92 Die oogenese einiger parthenogenetischer Orthocladiinen (Diptera). H Scholl author 1960 text Chromosoma 1960 11 380 401 Phylogenetic relationships between parthenogens and their sexual relatives: the possible routes to parthenogenesis in animals. J-C Simon author 2003 text Biological Journal of the Linnaean Society 2003 79 151 163 Importance of parthenogenesis in the evolution of insects. E Suomalainen author 1962 text Annual Review of Entomology 1962 7 349 366 Laboratory culture of chironomid species from the Hula Nature Preserve, Israel. D Wool author 1968 text Annales zoologici fennici 1968 5 153 154 Die Verwendung der imaginalscheiben zur Bestimmung des Entwicklungszustandes von Chironomus-larven. W Wülker author 1968 text Zeitschrift für Morphologie und Ökologie der Tiere 1968 62 363 388 P Yang author 2008 Assembly and significance of centrosome during parthenogenesis of insects. Chinese Journal of Cell Biology 2008–03. 2008 10.3897/compcytogen.v5i4.1356 https://compcytogen.pensoft.net/article/1731/ https://compcytogen.pensoft.net/article/1731/download/pdf/ https://compcytogen.pensoft.net/article/1731/download/xml/ Micropsectra sedna (Oliver, 1976) is a parthenogenetic midge from the Canadian Arctic. The parthenogenetic mechanism is apomictic thelytoky, with a restitutional division during oogenesis, as found in other parthenogenetic Chironomidae. It is triploid, with two similar chromosome sets, and the third is relatively dissimilar, pairing little with the diploid set. Two karyotypes were observed: a single individual with eight polytene elements in the salivary glands (3n=12), considered standard, while the majority of larvae showed only seven polytene chromosomes (3n=11). Hybrid speciation is considered likely, although chromosomal recombination following the origin of thelytoky has played some part in karyotype evolution. A single morphologically distinct larva was also found, which might be the donor of the haploid chromosome set. The apomictic restitutional system is compared to that of the other, independently derived, parthenogenetic Chironomids to assess the extent of similarity between species. text/html en_US Pensoft Publishers Chironomidae parthenogenesis polytene chromosomes hybridization chromosome recombination Cytogenetics of a parthenogenetic Arctic species of Micropsectra (Diptera, Chironomidae) Research Article

10.3897/compcytogen.v5i4.1149 2011-11-09 compcytogen

UEL, Brazil author Bitencourt, Jamille Universidade Estadual do Sudoeste da Bahia, Jequié, Brazil author Affonso, Paulo Roberto Universidade Estadual de Londrina, Londrina, Brazil author Giuliano-Caetano, L. Departamento de Biologia Geral, CCB, Universidade Estadual de Londrina, Londrina, Brazil author Dias, Ana Lúcia 2011-11-09 2011-11-09 2011 Comparative Cytogenetics 1993-078X 1993-0771 5 329 344 2011 Chromosomal diversification of reef fishes from genus Centropyge (Perciformes, Pomacanthidae). PRAM Affonso author 2005 text Genetica 2005 123 3 227 233 Improving Salmo salar karyotype: restriction enzyme and replication banding. M Albuín author 1994 text Cytobios 1994 78 143 152 A heteromorphic ZZ/ZW sex chromosome system in fish, genus Hypostomus (Loricariidae). RF Artoni author 1998 text Cytologia 1998 63 421 425 Nature and distribution of constitutive heterochromatin in fishes, genus Hypostomus (Loricariidae). RF Artoni author 1999 text Genetica 1999 106 3 209 214 Cytotaxonomic considerations on Hoplias lacerdae (Pisces, Erythrinidae). LAC Bertollo author 1978 text Brazilian Journal of Genetics 1978 1 103 120 The mechanism and pattern of banding induced by restriction endonucleases in human chromosomes. MS Bianchi author 1985 text Chromosoma 1985 91 2 131 136 JA Bitencourt author 2010 2010 karotypic characterization of turbot (Scophthalmus maximus) with conventional, fluorochrome and restriction endonuclease-banding techniques. C Bouza author 1994 text Marine Biology 1994 120 4 609 613 DNA-protein interactions and chromosome banding. GD Burkholder author 1977 text Experimental Cell Research 1977 106 2 251 262 Karyotypic characterization of Iheringichthys labrosus (Pisces, Pimelodidae): C-, G- and restriction endonuclease banding. RA Carvalho author 2005 text Genetics and Molecular Research 2005 4 4 663 667 The characterization of Muraena helena L. mitotic chromosomes: karyotype, C-banding, nucleolar organizer regions, and in situ digestion with restriction endonucleases. A Cau author 1988 text Cytogenetics and Cell Genetics 1988 47 4 223 226 Karyotype description of two species of Hypostomus (Siluriformes, Loricariidae) of the Planalto da Bodoquena, Brazil. SS Cereali author 2008 text Genetics and Molecular Research 2008 7 3 583 591 Physical chromosome mapping of repetitive DNA sequences in Nile tilapia Oreochromis niloticus: evidences for a differential distribution of repetitive elements in the sex chromosomes. IA Ferreira author 2008 text Micron 2008 39 4 411 418 Correlation between constitutive heterochromatin and restriction enzyme resistant chromatin in Arcyptera tornosi (Orthoptera). J Gosálvez author 1986 text Heredity 1986 59 173 180 Biogeography and comparative cytogenetics between two populations of Hoplias malabaricus (Bloch, 1794) (Ostariophysi, Erythrinidae) from coastal basins in the State of Bahia, Brazil. UP Jacobina author 2009 text Neotropical Ichthyology 2009 7 4 617 622 Improving beetle karyotype analysis: restriction endonuclease banding of Tenebrio molitor chromosomes. C Juan author 1990 text Heredity 1990 65 157 162 Restriction enzyme banding of mouse metaphase chromosomes. M Kaelbling author 1984 text Chromosoma 1984 90 2 128 132 Cytotaxonomy, heterochromatic polymorphism and natural triploidy of a species of Astyanax (Pisces, Characidae) endemic to the Iguaçu River Basin. DLZ Kantek author 2007 text Brazilian Archives ofBiology and Technology 2007 50 1 67 74 Heterochromatin characterization of four fish species of the family Loricariidae (Siluriformes). KF Kavalco author 2004 text Hereditas 2004 141 3 237 242 Karyotypic diversity and evolution of Loricariidae (Pisces, Siluriformes). KF Kavalco author 2005 text Heredity 2005 94 180 186 Yeast stimulation of bone marrow mitosis for cytogenetic investigations. MR Lee author 1980 text Cytogenetics and Cell Genetics 1980 26 36 40 A nomenclature for centromeric position on chromosomes. A Levan author 1964 text Hereditas 1964 52 201 220 Action of restriction endonucleases on the DNA and chromosomes of Muntiacus muntjak. A Lima-de-Faria author 1980 text Hereditas 1980 92 267 273 Restriction endonuclease banding of rainbow trout chromosomes. MA Lloyd author 1988 text Chromosoma 1988 96 2 171 177 Restriction endonuclease chromosome banding in Tapinoma nigerrimum (Hymenoptera, Formicidae). P Lorite author 1999 text Hereditas 1999 131 197 201 An analysis of coho salmon chromatin by means of C-banding, AG- and fluorochrome staining, and in situ digestion with restriction endonucleases. R Lozano author 1991 text Heredity 1991 66 403 409 R- and G-band patterns in Astyanax scabripinnis paranae (Pisces, Characiformes, Characidae). EL Maistro author 1999 text Genetics and Molecular Biology 1999 22 2 201 204 Cytogenetic analysis of A and B chromosomes of Prochilodus lineatus (Teleostei, Prochilodontidae) using different restriction enzyme banding and staining methods. EL Maistro author 2000 text Genetica 2000 108 2 119 125 Restriction endonuclease digestion and chromosome banding in the mosquito, Culiseta longiareolata (Diptera: Culicidae). A Marchi author 1988 text Heredity 1988 60 21 26 Inter- and intraspecific heterochromatin variation detected by restriction endonuclease digestion in two sibling species of the Anopheles maculipennis complex. A Marchi author 1990 text Heredity 1990 65 135 142 Chromatin organization and restriction nuclease activity on human methaphase chromosomes. R Mezzanotte author 1983 text Cytogenetics and Cell Genetics 1983 36 3 562 566 Some factors affecting the action of restriction endonucleases on human metaphase chromosomes. R Mezzanote author 1985 text Experimental Cell Research 1985 161 1 247 253 Q- and C-band chromosome markers in inbred strains of Mus musculus. DA Miller author 1976 text Genetics 1976 81 1 67 75 Chromosome localization of highly repetitive human DNAs and amplified ribosomal DNA with restriction enzymes. DA Miller author 1983 text Science 1983 219 4583 395 397 Different cytotypes in fishes of the genus Hypostomus Lacépède, 1803 (Siluriformes: Loricariidae) from Xingu river (Amazon region, Brazil. SSR Milhomem author 2010 text Comparative cytogenetics 2010 4 1 45 54 An alternative method for mitotic stimulation in fish cytogenetics. WF Molina author 2001 text Chromosome Science 2001 5 3 149 152 Chromosomal evidence of population subdivision in the freshwater fish Leporinus elongatus in the Upper Paraná River basin. WF Molina author 2008 text Genetics and Molecular Biology 2008 31 1 270 274 Analysis of constitutive heterochromatin by fluorochromes and in situ digestion with restriction enzymes in species of the group Callithrix argentata (Callitrichidae, Primates). JC Pieczarka author 1996 text Cytogenetics and Cell Genetics 1996 72 4 325 330 Cytogenetic characterization of Hypostomus nigromaculatus (Siluriformes: Loricariidae). M Rubert author 2008 text Neotropical Ichthyology 2008 6 1 93 100 Analysis of the structure and variability of nucleolar organizer regions of Salmo trutta by C-, Ag-, and restriction endonuclease banding. L Sánchez author 1990 text Cytogenetics and Cell Genetics 1990 54 1–2 6 9 Chromosomal heterochromatin differentiation in Salmo trutta with restriction enzymes. L Sánchez author 1991 text Heredity 1991 66 241 249 A simple technique for demonstrating centromeric heterochromatin. AT Sumner author 1972 text Experimental Cell Research 1972 75 304 306 A model for heterochromatin dispersion and the evolution of C-bands patterns. D Schweizer author 1987 text Chromosome Today 1987 9 61 74 First chromosome data on Steindachneridion scripta (Pisces, Siluriformes, Pimelodidae) from Brazilian rivers: Giemsa, CBG, G, and RE banding. AC Swarça author 2005 text Genetics and Molecular Research 2005 4 4 734 741 10.3897/compcytogen.v5i4.1149 https://compcytogen.pensoft.net/article/1730/ https://compcytogen.pensoft.net/article/1730/download/pdf/ https://compcytogen.pensoft.net/article/1730/download/xml/ Cytogenetic analyses using C-banding and chromosomal digestion by several restriction enzymes were carried out in four populations (named A, B, C and D) of Hypostomus prope unae (Loricariidae, Hypostominae) from Contas river basin, northeastern Brazil. These populations share 2n=76 and single NORs on the second metacentric pair but exclusive karyotype forms for each locality. Populations A and B presented conspicuous terminal and interstitial heterochromatic blocks on most of acrocentric chromosomes and equivalent to NORs with differences in both position and bearing pair. Population D showed evident marks at interstitial regions and interspersed with nucleolar region while population C presented interstitial and terminal heterochromatin segments, non-coincident with NORs. The banding pattern after digestion with the endonucleases Alu I, Bam HI, Hae III and Dde I revealed a remarkable heterogeneity within heterochromatin, allowing the identification of distinctive clusters of repeated DNA in the studied populations, besides specific patterns along euchromatic regions. The analysis using restriction enzymes has proved to be highly informative, characterizing population differences and peculiarities in the genome organization of H. prope unae. text/html en_US Pensoft Publishers C-banding heterochromatin ichthyofauna restriction enzymes Heterochromatin heterogeneity in Hypostomus prope unae (Steindachner, 1878) (Siluriformes, Loricariidae) from Northeastern Brazil Research Article

10.3897/compcytogen.v5i4.965 2011-11-09 compcytogen

Department of Agricultural Biotechnology, CSK Himachal Pradesh Agricultural University, Palampur, Palampur, India author Chahota, R. K. Laboratory of Plant Molecular Genetics, Division of Natural Sciences, Osaka, Japan author Mukai, Y. Molecular Cytogenetics and Tissue Culture Lab, Department of Crop Improvement, CSK, Himachal Pradesh Agricultural University, Palampur, India author Chaudhary, H.K. Molecular Cytogenetics and Tissue Culture Lab, Department of Crop Improvement, CSK, Himachal Pradesh Agricultural University, Palampur, India author Kishore, Naval Department of Agricultural Biotechnology, CSK Himachal Pradesh, India author Sharma, T.R. 2011-11-09 2011-11-09 2011 Comparative Cytogenetics 1993-078X 1993-0771 5 345 353 2011 Variation in rDNA locus number and position among legume species and detection of 2 linked rDNA loci in the model Medicago truncatula by FISH. M Abirached-Darmency author 2005 text Genome 2005 48 556 561 Genome differentiation in Aegilops 2. Physical mapping of 5S & 18S-26S rDNA gene families in diploid species. ED Badaeva author 1996 text Genome 1996 39 1150 1158 Phylogenetic and genetic relationships in Setaria italica and its close relatives based on the molecular diversity and chromosomal organisation of 5S and 18S-5.8S-25S rDNA genes. A Benabdelmouna author 2001 text Theoretical And Applied Genetics 2001 103 668 667 Physical mapping of 5S and 18S-25S rDNA and repetitive DNA sequences in Aegilops umbellulata. A Castilho author 1995 text Genome 1995 38 91 96 Physical mapping of repetitive DNA sequences and 5S & 18S-26S rDNA in five wild species of the genus Hordeum. A De Bustos author 1996 text Chromosome Research 1996 4 491 499 The structure and control of expression of ribosomal RNA genes. RB Flavell author 1986 text Oxf Surv Plant Molecular and Cell Biology 1986 3 252 274 Variability in rDNA loci in the genus Oryza detected through fluorescence in situ hybridisation. K Fukui author 1994 text Theoretical And Applied Genetics 1994 103 839 845 Cloning and characterization of ribosomal RNA genes from wheat and barley. WL Gerlach author 1979 text Nucleic Acids Research 1979 7 1869 1885 Chromosomal localization of 18S and 5S rNA genes in garlic, Alium sativum. M Hizume author 1994 text Chromosome Information Service 1994 56 6 8 Nucleotide sequence of a highly repeated DNA sequence and its chromosomal localization in Allium fistulosum. K Irifune author 1995 text Theoretical And Applied Genetics 1995 90 312 316 . Physical and genetic mapping in the grasses Lolium perenne and Festuca pratensis. L King author 2002 text Genetics 2002 161 315 324 FISH mapping of the 5S and 18S-28rDNA loci in different species of Glycine. P Krishnan author 2001 text Journal of Heredity 2001 92 282 287 Somatic chromosome karyotype of tomato based on in situ hybridization on the TAGI satellite repeat. NLV Lapitan author 1989 text Genome 1989 32 992 998 In situ chromosomal localization of rDNA sites in “Safed Musli” chlorophytum Ker-Gawl and their physical measurement by Fiber FISH. UC Lavania author 2005 text Journal of Heredity 2005 96 155 160 Localization of tandemly repeated DNA sequences in Arabidopsis thaliana. J Maluszynska author 1991 text The Plant Journal 1991 1 159 166 Trends in site number change of rDNA loci during polyploid evolution in Sanguisorba (Rosaceae). M Mishima author 2002 text Chromosoma 2002 110 550 558 Perspectives in molecular cytogenetics of wheat. Memorial issue. Y Mukai author 2005 text Wheat Information Services 2005 100 17 30 Physical mapping of the 5S rDNA multigene family in common wheat. Y Mukai author 1990 text Journal of Heredity 1990 81 290 295 Physical mapping of the 18S.27S rRNA multigene family in common wheat: identification of a new locus. Y Mukai author 1991 text Chromosoma 1991 100 71 78 The correlation between rDNA copy number and genome size in eukaryotes. CD Prokopowich author 2003 text Genome 2003 46 48 50 Detection of variable number of 18S-5.8S-26S and 5S rDNA loci by fluorescent in situ hybridization in diploid and tetraploid Arachis species. SN Raina author 1999 text Genome 1999 42 52 59 Identification of a tandemly repeated DNA sequence using combined RAPD and FISH in Welsh onion (Allium fistulosum). BB Seo author 1999 text Korean Journal of Biological Science 1999 3 69 72 Repetitive DNA sequences from polyploid Elymus trachycaulus and the diploid progenitor species-detection and genomic affinity of Ellymus chromatin added to wheat. H Tsujimoto author 1991 text Genome 1991 34 782 789 Physical mapping of the 18S ribosomal RNA gene of peach (Prunus persica (L) Batshc) chromosomes by fluorescence in situ hybridization. M Yamamoto author 1999 text Breeding Science 1999 49 49 51 Molecular cytogenetic characterization of the Antirrhinum majus genome. D Zhang author 2005 text Genetics 2005 169 325 335 10.3897/compcytogen.v5i4.965 https://compcytogen.pensoft.net/article/1729/ https://compcytogen.pensoft.net/article/1729/download/pdf/ https://compcytogen.pensoft.net/article/1729/download/xml/ The fluorescent in situ hybridization (FISH) technique has been applied to somatic chromosomes in the medicinally important species, Bunium persicum, to elucidate its karyotypes. The bicolour FISH technique involving 18S-5.8S-26S and 5S ribosomal RNA genes as probes was used to assign physical localization and measurement of rDNA sites on homologous pairs of chromosomes. The two 18S-5.8S-26S rRNA gene sites were at the terminal regions of the short arms of the chromosomes 1 and 2 involving NOR region of chromosome 1. The 5S rDNA sites were found on subtelomeric region of the long arm of the chromosome number 5 and at interstitial regions of the short arm of chromosome 7. Based on direct visual analysis of chromosome length, morphology and position of FISH signals, a pioneer attempt has been made to construct metaphase karyotype in B. persicum, an endangered medicinal plant of North Western Himalayas. text/html en_US Pensoft Publishers FISH Karyotypes Bunium persicum black cumin Karyotyping and in situ chromosomal localization of rDNA sites in black cumin Bunium persicum (Boiss) B. Fedtsch,1915 (Apiaceae) Research Article

10.3897/compcytogen.v5i4.2307 2011-11-09 compcytogen

Bulgarian Academy of Sciences, Sofia, Bulgaria author Grozeva, Snejana https://orcid.org/0000-0003-3196-8068 Zoological Institute RAS, author Kuznetsova, Valentina Zoological Institute RAS, St.Petersburg, author Anokhin, Boris https://orcid.org/0000-0002-4110-6704 2011-11-09 2011-11-09 2011 Comparative Cytogenetics 1993-078X 1993-0771 5 355 374 2011 Chromosomal location of heterochromatin and 45S rDNA sites in four South American triatomines (Heteroptera: Reduviidae). VB Bardella author 2010 text Comparative Cytogenetics 2010 4 2 141 149 Heterochromatin heteromorphism in Holhymenia rubiginosa (Heteroptera, Coreidae). MJ Bressa author 2008 text European Journal of Entomology 2008 105 65 72 Sex chromosome evolution in cotton stainers of the genus Dysdercus (Heteroptera: Pyrrhocoridae). MJ Bressa author 2009 text Cytogenetic and Genome Research 2009 125 292 305 H Busch author 1970 The Nucleolus. Academic Press, NewYork. 1970 Male meiotic behaviour and nucleolus organizing regions in Camptischium clavipes (Fabr.) (Coreidae, Heteroptera) analyzed by fluorescent banding and in situ hybridization. MV Cattani author 2004 text Caryologia 2004 57 267 273 Methyl green is a substitute for distamycin A in the formation of distamycin A/DAPI C-bands. TA Donlon author 1983 text Human Genetics 1983 65 144 146 Phylogenetic distribution of TTAGG telomeric repeats in insects. R Frydrychová author 2004 text Genome 2004 47 163 78 Das Heterochromatin der Geschlechtschromosomen bei Heteropteren. L Geitler author 1939 text Chromosoma 1939 1 197 229 The occurrence of m-chromosomes in the karyotypes of six species of the Oxycareninae (Heteroptera, Lygaeidae). SM Grozeva author 1995 text Folia biologica (Kraków) 1995 43 55 59 A model bug species Oxycarenus lavaterae (Insecta, Heteroptera, Lygaeidae) chronically treated with Pb ions. S Grozeva author 2004 text Acta Entomologica Bulgarica 2004 10 19 22 Bed bug cytogenetics: karyotype, sex chromosome system, FISH mapping of 18S rDNA, and male meiosis in Cimex lectularius Linnaeus, 1758 (Heteroptera: Cimicidae). S Grozeva author 2010 text Comparative Cytogenetics 2010 4 151 160 First evidence of sex chromosomes pre-reduction in male meiosis in the Miridae bugs (Heteroptera). S Grozeva author 2006 text Folia biologica (Kraków) 2006 54 9 12 Cytogenetic Studies of Bryocorinae true bugs (Heteroptera, Miridae). S Grozeva author 2008a text Acta Zoologica Bulgarica Suppl 2008a 2 61 70 S Grozeva author 2008b Cytotaxonomy of two Cremnocephalus species (Heteroptera: Miridae). In: S Grozeva, N Simov (Eds) Advances in Heteroptera Research: Festschrift in Honour of 80th Anniversary of Michail Josifov, Pensoft Publishers, Sofia-Moscow, 171–179. 2008b Karyotaxonomy of some European Macrolophus species(Heteroptera, Miridae). S Grozeva author 2007 text Mainzer Naturwissenschaftliches Archiv/Beiheft 2007 31 81 87 Geographical, spatial and temporal variability in the balanced polymorphism of Philaenus spumarius. O Halkka author 1964 text Heredity 1964 19 383 401 Über Spermatogenese und deren Beziehung zur Eientwicklung bei Pyrrhocoris apterus L. H Henking author 1891 text Zeit schrift für wissenschaftliche Zoologie 1891 51 685 736 Silver staining as an indicator of active ribosomal genes. HR Hubbell author 1985 text Stain Technology 1985 60 285 294 VG Kuznetsova author 2011 Cytogenetic characterization of the true bug infraorder Cimicomorpha (Heteroptera): a review. Zookeys (in press). 2011 Cytotaxonomy of Miridae and Nabidae (Hemiptera). D Leston author 1957 text Chromosoma 1957 8 609 616 Repeated genes in eukaryotes. EO Long author 1980 text Annual Review of Biochemistry 1980 49 727 764 What genes and chromosomes say about the origin and evolution of insects and other arthropods. VA Lukhtanov author 2010 text Russian Journal of Genetics 2010 46 1115 1121 Differential characterization of holokinetic chromosomes in triatomines (Heteroptera, Triatominae) using different staining techniques and fluorescent in situ hybridization. A Morielle-Souza author 2007 text Genetics and Molecular Research 2007 6 713 720 Achiasmatic male meiosis of collochore type in the heteropteran family Miridae. S Nokkala author 1986 text Hereditas 1986 105 193 197 Holocentric chromosomes in meiosis. I. Restriction of the number of chiasmata in bivalents. S Nokkala author 2004 text Chromosome Research 2004 12 733 739 Identification of a pentanucleotide telomeric sequence, (TTAGG)n, in the silkworm Bombyx mori and in other insects. S Okazaki author 1993 text Molecular and Cell Biology 1993 13 1424 1432 Cytogenetics and Genome Evolution in the Subfamily Triatominae (Hemiptera, Reduviidae). F Panzera author 2010 text Cytogenetic and Genome Research 2010 128 77 87 Classical and molecular cytogenetics in Heteroptera. AG Papeschi author 2006 text Research Advances in Entomology 2006 1 1 9 Behaviour of ring bivalents in holocentric systems: Alternative sites of spindle attachment in Pachylis argentinus and Nezara viruda (Heteroptera). AG Papeschi author 2003 text Chromosome Research 2003 11 725 733 Cytogenetic analysis using quantitative, high sensitivity, fluorescence hybridization. D Pinkel author 1986 text In: Proceedings of the National Academy of Sciences of the United States of America (PNAS) 1986 83 2934 2938 Male meiosis, heterochromatin characterization and chromosomal location of rDNA in Microtomus lunifer (Berg, 1900) (Hemiptera, Reduviidae, Hammacerinae). MG Poggio author 2011 text Comparative Cytogenetics 2011 5 1 22 Meiosis and fluorescent banding in Edessa meditabunda and Eurydema rufomarginata (Heteroptera: Pentatomidae: Edessinae). P Rebagliati author 2003 text European Journal of Entomology 2003 100 11 18 TTAGG telomeric repeats in chromosomes of some insects and other arthropods. K Sahara author 1999 text Chromosome Research 1999 7 449 460 Abhandlungen über haploide Chromosomengarnituren in den Samendrüsen der Hemiptera. F Schachow author 1932 text Anatomischer Anzeiger 1932 75 1 46 T Schwarzacher author 2000 Practical in situ Hybridization. BIOS. Scientific Publishers Ltd. Oxford, UK. 338 pp. 2000 Reverse fluorescent chromosome banding with chromomycin and DAPI. D Schweizer author 1976 text Chromosoma 1976 58 307 324 Localization of rDNA sites in holocentric chromosomes of three species of triatomines (Heteroptera, Triatominae). GD Severi-Aguiar author 2005 text Genetics and Molecular Research 2005 4 704 709 Meiosis aspects and nucleolar activity in Triatoma vitticeps (Triatominae, Heteroptera). GD Severi-Aguiar author 2006 text Genetica 2006 126 141 151 N Ueshima author 1979 Hemiptera II: Heteroptera. Animal Cytogenetics 3: Insecta 6. 1979 The evolutionary origin of insect telomeric repeats, (TTAGG)n. M Vítková author 2005 text Chromosome Research 2005 13 145 156 Systematics and Evolution of Heteroptera: 25 Years of Progress. C Weirauch author 2011 text Annual Review of Entomology 2011 56 487 510 Cariologia compartada de laguns Hemipteros Heteropteros Pentatomideos e Coreideos). A Xavier author 1945 text Memórias e Estudos do Museu Zoológico da Universidade de Coimbra 1945 163 1 105 10.3897/compcytogen.v5i4.2307 https://compcytogen.pensoft.net/article/1728/ https://compcytogen.pensoft.net/article/1728/download/pdf/ https://compcytogen.pensoft.net/article/1728/download/xml/ Eight species belonging to five true bug families were analyzed using DAPI/CMA3-staining and fluorescence in situ hybridization (FISH) with telomeric (TTAGG)n and 18S rDNA probes. Standard chromosomal complements are reported for the first time for Deraeocoris rutilus (Herrich-Schäffer, 1838) (2n=30+2m+XY) and D. ruber (Linnaeus, 1758) (2n=30+2m+XY) from the family Miridae. Using FISH, the location of a 18S rDNA cluster was detected in these species and in five more species: Megaloceroea recticornis (Geoffroy, 1785) (2n=30+XY) from the Miridae; Oxycarenus lavaterae (Fabricius, 1787) (2n=14+2m+XY) from the Lygaeidae s.l.; Pyrrhocoris apterus (Linnaeus, 1758) (2n=22+X) from the Pyrrhocoridae; Eurydema oleracea (Linnaeus, 1758) (2n=12+XY) and Graphosoma lineatum (Linnaeus, 1758) (2n=12+XY) from the Pentatomidae. The species were found to differ with respect to location of a 18S rRNA gene cluster which resides on autosomes in O. lavaterae and P. apterus, whereas it locates on sex chromosomes in other five species. The 18S rDNA location provides the first physical landmark of the genomes of the species studied. The insect consensus telomeric pentanucleotide (TTAGG)n was demonstrated to be absent in all the species studied in this respect, D. rutilus, M. recticornis, Cimex lectularius Linnaeus, 1758 (Cimicidae), E. oleracea, and G. lineatum, supporting the hypothesis that this motif was lost in early evolution of the Heteroptera and secondarily replaced with another motif (yet unknown) or the alternative telomerase-independent mechanisms of telomere maintenance. Dot-blot hybridization analysis of the genomic DNA from C. lectularius, Nabis sp. and O. lavaterae with (TTAGG)n and six other telomeric probes likewise provided a negative result. text/html en_US Pensoft Publishers Karyotypes meiosis FISH 18S rDNA telomeres dot-blot Deraeocoris Megaloceroea Nabis Cimex Oxycarenus Pyrrhocoris Eurydema Graphosoma Heteroptera Karyotypes, male meiosis and comparative FISH mapping of 18S ribosomal DNA and telomeric (TTAGG)n repeat in eight species of true bugs (Hemiptera, Heteroptera) Research Article

10.3897/compcytogen.v5i5.2116 2011-12-22 compcytogen

Zoological Institute, Russian Academy of Sciences, St. Petersburg, author Gavrilov-Zimin, Ilya https://orcid.org/0000-0003-1993-5984 2011-12-22 2011-12-22 2011 Comparative Cytogenetics 1993-078X 1993-0771 5 375 390 2011 Lecanoid chromosome behavior in three more families of the Coccoidea (Homoptera). SW Brown author 1959 text Chromosoma (Berlin) 1959 10 278 300 The Comstockiella system of chromosome behavior in the armored scale insects (Coccoidea: Diaspididae). SW Brown author 1963 text Chromosoma (Berlin) 1963 14 360 406 Chromosomal survey of the armored and palm scale insects (Coccoidea: Diaspididae and Phoenicococcidae). SW Brown author 1965 text Hilgardia 1965 36 189 294 Chromosome systems of the Eriococcidae (Coccoidea-Homoptera). 1. A survey of several genera. SW Brown author 1967 text Chromosoma (Berlin) 1967 22 126 150 Meiosis in the male of Puto albicans (Coccoidea-Homoptera). SW Brown author 1968 text Chromosoma (Berlin) 1968 24 210 232 Mealybugs of the genera Planococcus Ferris and Crisicoccus Ferris (Sternorrhyncha: Pseudococcidae) of Russia and adjacent countries. EM Danzig author 2010 text Zoosystematica Rossica 2010 19 1 39 49 On chromosomal sets in some coccids (Homoptera, Coccoidea). EM Drozdovsky author 1966 text Entomologicheskoe Obozrenie 1966 45 4 712 714 Taxonomic and cytogenetic studies of scale insects (Homoptera: Coccinea) of European Russia. IA Gavrilov author 2004 text Proceedings of Zoological Institute of Russian Academy of Sciences. 2004 300 77 82 IA Gavrilov author 2005 Systematics and cytogenetics of scale insects (Homoptera: Coccinea) of European Russia. 2005 A catalogue of chromosomal numbers and genetic systems of scale insects (Homoptera: Coccinea) of the world. 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E Tremblay author 1961 text Portici 1961 19 215 262 10.3897/compcytogen.v5i5.2116 https://compcytogen.pensoft.net/article/1741/ https://compcytogen.pensoft.net/article/1741/download/pdf/ https://compcytogen.pensoft.net/article/1741/download/xml/ New cytogenetic data are reported for 17 species from 15 genera of the families Pseudococcidae, Eriococcidae, Kermesidae, and Coccidae. Twelve species and 6 genera (Peliococcopsis Borchsenius, 1948, Heterococcopsis Borchsenius, 1948, Heliococcus Šulc, 1912, Trabutina Marchal, 1904, Lecanopsis Targioni Tozzetti, 1868, and Anapulvinaria Borchsenius, 1952) were studied cytogenetically for the first time. The taxonomic problems in the genera Trionymus Berg, 1899, Acanthopulvinaria Borchsenius, 1952 and Rhizopulvinaria Borchsenius, 1952 are discussed based on karyotype characters. Two chromosomal forms (cryptic species) of Acanthopulvinaria orientalis (Nasonov, 1908), 2n=18 and 2n=16 were discovered. text/html en_US Pensoft Publishers scale insects karyotypes chromosome numbers karyosystematics New cytogenetic data for some Palaearctic species of scale insects (Homoptera, Coccinea) with karyosystematic notes Research Article

10.3897/compcytogen.v5i5.2015 2011-12-22 compcytogen

Faculty of Sciences - University of Lisbon, Lisbon, Portugal author Rampin, Massimiliano Department of Biological Applications and Technology, University of Ioannina, Ioannina, Greece author Gkenas, Christos Department of Environmental Sciences, Informatics and Statistics, Ca’ Foscari University of Venice, Venice, Italy author Malavasi, Stefano CNR-Institute of Marine Sciences, Venice, Italy author Libertini, Angelo 2011-12-22 2011-12-22 2011 Comparative Cytogenetics 1993-078X 1993-0771 5 391 396 2011 Animal Genome Size Database author 2009 http://www.genomesize.com 2009 Nucleolar organizer (NOR) location and cytotaxonomic implications in six species of gobiid fishes (Perciformes, Gobiidae). V Caputo author 1998 text Italian Journal of Zoology 1998 65 93 99 AJ Crivelli author 2006 Economidichthys pygmaeus. In: IUCN 2008. 2008 IUCN Red List of Threatened Species. 2006 PS Economidis author 1991 Check list of freshwater fishes of Greece: recent status of threats and protection. 1991 Freshwater fishes of Greece. PS Economidis author P Legakis A and Maragkou author 2009 text Hellenic Zoological Society, Athens, Greece 2009 86 159 An overview of marine fish cytogenetics. PM Jr Galetti author 2000 text Hydrobiologia 2000 420 55 62 Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. WM Howell author 1980 text Cellular and Molecular Life Sciences 1980 36 1014 1015 Paleoclimatic history and vicariant speciation in the “sand goby” group (Gobiidae, Teleostei). T Huyse author 2004 text Molecular Phylogenetics and Evolution 2004 32 324 336 M Klinkhardt author 1995 Database of fish chromosomes. 1995 Fish fauna in a protected Greek lake: biodiversity, introduced fish species over a 80-year period and their impacts on the ecosystem. ID Leonardos author 2008 text Ecology of Freshwater Fish 2008 17 165 173 Nomenclature for centromeric position on chromosomes. A Levan author 1964 text Hereditas 1964 52 201 220 A cytogenetic study of the common sole, Solea solea, from the Northern Adriatic Sea. A Libertini author 2003 text Chromosome Science 2003 6 63 66 Classical and molecular cytogenetic characterization of allochthonous European bitterling Rhodeus amarus (Cyprinidae, Acheilognathinae) from Northern Italy. A Libertini author 2008 text Genes and Genetic Systems 2008 83 5 417 422 Interspecific variation of acoustic signals in Mediterranean gobies (Perciformes, Gobiidae): comparative analysis and evolutionary outlook. S Malavasi author 2008 text Biological Journal of the Linnean Society 2008 93 4 763 778 The affinities of European sand gobies (Teleostei: Gobiidae). SI McKay author 1997 text Journal of Natural History 1997 31 1457 1482 PJ Miller author 1990 The endurance of endemism: the Mediterranean freshwater gobies and their prospects for survival. Journal of Fish Biology 37 (Suppl. A):145–156. 1990 Arrangements and rearrengements of sequences flanking the two types of rDNA insertion in D. melanogaster. H Roiha author 1981 text Nature 1981 290 49 53 Reverse fluorescent chromosome banding with chromomycin and DAPI. D Schweizer author 1976 text Chromosoma 1976 58 307 324 10.3897/compcytogen.v5i5.2015 https://compcytogen.pensoft.net/article/1740/ https://compcytogen.pensoft.net/article/1740/download/pdf/ https://compcytogen.pensoft.net/article/1740/download/xml/ A cytogenetic study was carried out on the chromosomes and the nuclear DNA content of the freshwater goby Economidichthys pygmaeus (Pisces, Gobiidae). The species is characterized by a 2n=46 karyotype consisting of 12 submetacentric and 11 subtelocentric chromosome pairs (NF=70). Major (45S) rDNA genes are terminal-centromeric located on the short arm of a single medium-small sized submetacentric pair as assessed by in situ hybridization, CMA3 staining, and Ag-NOR banding. The haploid (C-value) nuclear DNA content is 0.93±0.003 picograms. The cytogenetical data of E. pygmaeus were compared with those ones already available for other related gobies. text/html en_US Pensoft Publishers Goby fish karyotype fluorescent in situ hybridization genome size A cytogenetical study on Economidichthys pygmaeus Holly, 1929 (Pisces, Gobiidae), an endemic freshwater goby from Western Greece Short Communication

10.3897/compcytogen.v5i5.1589 2011-12-22 compcytogen

Universidade Federal de São Carlos, São Carlos, Brazil author Rubert, Marceléia https://orcid.org/0000-0002-2832-5929 Universidade Estadual de Maringá, Parana, Brazil author Rosa, Renata https://orcid.org/0000-0003-4258-7244 Pontifícia Universidade Católica, Brazil author Jerep, Fernando Camargo Universidade Federal de São Carlos, Brazil author Bertollo, Luis Antônio Carlos Universidade Estadual de Londrina, Londrina, Brazil author Giuliano-Caetano, L. 2011-12-22 2011-12-22 2011 Comparative Cytogenetics 1993-078X 1993-0771 5 397 410 2011 AL Alves author 2000 Análise da evolução dos gêneros da subfamilia Hemipsilichthiinae (Ostariophysi, Siluriformes, Loricariidae) com base em caracteres cromossômicos e de DNA mitocondrial. Ph.D. Dissertation, Botucatu, Brazil: Instituto de Biociências, Universidade Estadual Paulista. 2000 Karyotypic relationships among the tribes of Hypostominae (Siluriformes: Loricariidae) with description of XO sex chromosome system in a Neotropical fish species. AL Alves author 2006 text Genetica 2006 128 1 9 Phylogeneticrelationshipsofsuckermoutharmouredcatfishes(Loricariidae) with emphasis on the Hypostominae and Ancistrinae. JW Armbruster author 2004 text Zoological Journal of the Linnean Society 2004 141 1 80 CytogeneticstudiesonHypostominae(Pisces,Siluriformes,Loricariidae).Considerationsonkaryotypeevolutioninthegenus Hypostomus. RF Artoni author 1996 text Caryologia 1996 49 81 90 Nature and distribution of constitutive heterochromatinin fishes, genus Hypostomus (Loricariidae). RF Artoni author 1999 text Genetica 1999 106 209 214 Trends in the karyotype evolution of Loricariidae fish(Siluriformes). RF Artoni author 2001 text Hereditas 2001 134 201 210 A heteromorphic ZZ/ZW sex chromosomesystem in fish, genus Hypostomus (Loricariidae). RF Artoni author 1998 text Cytologia 1998 63 421 425 Cytotaxonomic considerations on Hoplias lacerdae (Pisces, Erythrinidae). LAC Bertollo author 1978 text Brazilian Journalof Genetics 1978 1 103 120 FM Camilo author 2004 Estudos citogenéticos em algumas espécies de peixes da família Loricariidae pertencentes à bacia do rio Piracicaba. Ph.D. Dissertation, São Carlos, Brazil: Departamento de Genética e Evolução, Universidade Federal de São Carlos. 2004 A centromeric satellite DNA may be involved in heterochromatin compactness in gobiid fishes. A Canapa author 2002 text Chromosome Research 2002 10 297 304 SS Cereali author 2006 Estudos citogenéticos de Loricariidae (Siluriformes) do Planalto da Bodoquena – Mato Grosso do Sul. Ph.D. Dissertation, Londrina, Brazil: Departamento de Biologia Geral, Universidade Estadual deLondrina. 2006 Karyotype description of two species of Hypostomus (Siluriformes, Loricariidae) of the Planalto da Bodoquena, Brazil. SS Cereali author 2008 text Genetics and Molecular Research 2008 7 583 591 Nuclear DNA content in 20 species of Siluriformes (Teleostei, Ostaryophysi) from the Neotropical region. PC Fenerich author 2004 text Genetics and Molecular Biology 2004 27 3 350 354 CMA3-banding pattern and fluorescence in situ hybridization with 18S rRNA genes in zebrafish chromosomes. E Gornung author 1997 text Chromosome Research 1997 5 40 46 Mapping of 18S and 5S ribosomal RNA genes in the fish Prochilodus argenteus Agassiz, 1829 (Characiformes, Prochilodontidae). T Hatanaka author 2004 text Genetica 2004 122 239 244 Controled silver staining of nucleous organizer regionswith a protective colloidal developer: a 1-step method. WM Howell author 1980 text Experientia 1980 36 1014 1015 Heterochromatin characterization of four fish species of the family Loricariidae (Siluriformes). KF Kavalco author 2004 text Hereditas 2004 141 237 242 Karyotypic diversity andevolution of Loricariidae (Pisces, Siluriformes). KF Kavalco author 2005 text Heredity 2005 94 180 186 EO Mendes Neto author 2008 Estudos citogenéticos em algumas espécies de Loricariidae (Teleostei, Siluriformes) da região de transposição do rio Piumhi para o rio São Francisco. Ph.D. Dissertation, São Carlos, Brazil: Departamento de Genética e Evolução, Universidade Federal de São Carlos. 2008 Karyotypic studies of some species of thefamily Loricariidae (Pisces). JL Michele author 1977 text Cytologia 1977 42 539 546 Phylogenetic relationships of the Loricariidae (Siluriformes) based on mitochondrial rRNA gene sequences. JI Montoya-Burgos author LR Malabarba author 1998 text Porto Alegre 1998 363 374 On the diploid state of the fish order Ostariophysi. J Muramoto author 1968 text Chromosoma 1968 24 59 66 Localization of repetitive DNAs to zebrafish (Danio rerio) chromosomes by fluorescence in situ hybridization (FISH). RB Phillips author 2000 text Chromosome Research 2000 8 27 35 Chromosomal characteristics of ribosomal DNA in the primitive semionotiform fish, longnose gar Lepisosteus osseus. P Ráb author 1999 text Chromosome Research 1999 7 475 480 Delturinae, a new loricariid catfish subfamily (Teleostei, Siluriformes), with revisions of Delturus and Hemipsilichthys. RE Reis author 2006 text Zoological Journal of the Linnean Society 2006 147 277 299 Cytogenetic characterization of Hypostomus nigromaculatus (Siluriformes: Loricariidae). M Rubert author 2008 text Neotropical Ichtyhology 2008 6 93 100 Simultaneous fluorescent staining of R bands and specific heterochromatic regions (DA-DAPI bands) in human chromosomes. D Schweizer author 1980 text Cytogenetics and Cell Genetics 1980 27 190 193 Asimpletechiniquefordemonstratingcentromericheterochromatin. AT Sumner author 1972 text Experimental Cell Research 1972 75 304 306 Heteromorphism of rDNA size in Pinirampus pirinampu (Pisces: Pimelodidae) detected by in situ hybridization. AC Swarça author 2001 text Cytologia 2001 66 275 278 Subfamily Hypostominae. C Weber author RE Reis author 2003 text Porto Alegre 2003 351 372 Allozyme relationships in hypostomines (Teleostei: Loricariidae) from the Itaipu Reservoir, Upper Rio Paraná basin, Brazil. CH Zawadzki author 2005 text Genetica 2005 123 271 283 Two new species of Hypostomus Lacépède (Teleostei: Loricariidae) from the upper rio Paraná basin, Central Brazil. CH Zawadzki author 2008 text Neotropical Ichtyhology 2008 6 403 412 10.3897/compcytogen.v5i5.1589 https://compcytogen.pensoft.net/article/1739/ https://compcytogen.pensoft.net/article/1739/download/pdf/ https://compcytogen.pensoft.net/article/1739/download/xml/ Cytogenetic analyses were performed on fishes of the genus Hypostomus (H. ancistroides (Ihering, 1911), H. strigaticeps (Regan, 1908), H. regani (Ihering, 1905), and H. paulinus (Ihering, 1905)) from the seven tributaries of the Paranapanema River Basin (Brazil) by means of different staining techniques (C-, Ag-, CMA3- and DAPI-banding) and fluorescence in situ hybridization (FISH) to detect 18S rDNA sites. All species showed different diploid numbers: 2n=68 (10m+26sm+32st-a) in H. ancistroides, 2n=72 (10m+16sm+46st-a) in H. strigaticeps, 2n=72 (10m+18sm+44st-a) in H. regani and 2n=76 (6m+16sm+54st-a) in H. paulinus. Ag-staining and FISH revealed various numbers and locations of NORs in the group. NORs were usually located terminally on the subtelocentric/acrocentric chromosomes: on the long arm in H. strigaticeps (2 to 4) and H. paulinus (2); and on the short arm in H. ancistroides (2 to 8) and H. regani (2 to 4). Conspicuous differences in heterochromatin distribution and composition were found among the species, terminally located in some st-a chromosomes in H. ancistroides, H. strigaticeps, and H. paulinus, and interstitially dispersed in most st-a chromosomes, in H. regani. The fluorochrome staining indicated that different classes of GC and/or AT-rich repetitive DNA evolved in this group. Our results indicate that chromosomal rearrangements and heterochromatin base-pair composition were significant events during the course of differentiation of this group. These features emerge as an excellent cytotaxonomic marker, providing a better understanding of the evolutionary mechanisms underlying the chromosomal diversity in Hypostomus species. text/html en_US Pensoft Publishers loricariid catfishes chromosome banding NORs fluorochromes fluorescence in situ hybridization (FISH) Cytogenetic characterization of four species of the genus Hypostomus Lacépède, 1803 (Siluriformes, Loricariidae) with comments on its chromosomal diversity Research Article

10.3897/compcytogen.v5i5.1562 2011-12-22 compcytogen

Universidade Federal do Amazonas, Manaus, Amazonas, Brazil author Souza, Eduardo Instituto Nacional de Pesquisas da Amazônia - INPA, Brazil author Ribeiro, Leila Instituto Nacional de Pesquisas da Amazônia, Manaus, Brazil author Feldberg, Eliana https://orcid.org/0000-0002-2962-4541 Universidade Federal do Amazonas - UFAM, Brazil author Farias, Izeni Federal University of Amazonas, Manaus, Brazil author Hrbek, Tomas https://orcid.org/0000-0003-3239-7068 Univ Fed Amazonas, Inst Ciencias Biol, Lab Genet Anim, Manaus, Amazonas Brazil, Manaus, Brazil author Gross, Maria 2011-12-22 2011-12-22 2011 Comparative Cytogenetics 1993-078X 1993-0771 5 411 422 2011 Morphologically differentiated sex chromosomes in neotropical freshwater fish. LF Almeida-Toledo author 2001 text Genetica 2001 111 91 100 VV Arkhipchuk author 1999 Chromosome database. Database of Dr. Victor Arkipchuk. 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WJEM Costa author LR Malabarba author 1998 text Edipucrs, Porto Alegre 1998 537 560 Fluviphylax palikur: A new poeciliid from the Rio Oiapoque basin, northern Brazil (Cyprinodontiformes: Cyprinodontoidei), with comments on miniaturization in Fluviphylax and other neotropical freshwater fishes. WJEM Costa author 1999 text Copeia 1999 1999 1027 1034 Cytogenetic investigation in livebearing toothcarps (Pisces, Poeciliidae). In: Oliveira C, LF Almeida-Toledo, F Forest, HA Britski, AS Toledo-Filho (1988) Chromosome forumulae of neotropical freshwater fishes. W Feichtinger author 1988 text Revista Brasileira de Genética 1988 11 577 624 JS Fonseca author 1982 Curso de estatística. Atlas, São Paulo, 1–286. 1982 Chromosome studies in Poecilia latipunctata with NOR polymorphism as shown by silver nitrate and chromomycin A3 staining. 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JJ Scheel author 1972 text Journal of Zoological Systematic and Evolution Research 1972 10 180 209 L Sola author 1990 Cytogenetics of bisexual/unisexual Poecilia. I. C-bands, AgNOR polymorphisms and sex chromosomes in three populations of Poecilia latipinna. Cytogenetics and Cell Genetics53 148–154. 1990 Cytogenetics of bisexual/unisexual species of Poecilia. IV. Sex chromosomes, sex chromatin compositions and Ag-NOR polymorphisms in Poecilia latipinna: a population from Mexico. L Sola author 1993 text Heredity 1993 70 67 71 ER Souza author 2008 Filogeografia do gênero neotropical Fluviphylax (Cyprinodontiformes: Poeciliidae) das bacias do Amazonas e do Orinoco. 2008 A simple technique for demonstrating centromeric heterochromatin. AT Sumner author 1972 text Experimental Cell Research 1972 75 304 306 Esterase-D and chromosome patterns in Central Amazon piranha (Serrasalmus rhombeus Linnaeus, 1766) from lake Catalão. AS Teixeira author 2006 text Genetics and Molecular Biology 2006 29 498 502 M Völker author 2006 text Cytogenetic Genome and Research 2006 115 70 83 10.3897/compcytogen.v5i5.1562 https://compcytogen.pensoft.net/article/1738/ https://compcytogen.pensoft.net/article/1738/download/pdf/ https://compcytogen.pensoft.net/article/1738/download/xml/ The genus Fluviphylax Whitley, 1965 is comprized of five valid species (F. pygmaeus Myers et Carvalho, 1955, F. zonatus, F. simplex, F. obscurus Costa, 1996, and F. palikur Costa et Le Bail, 1999), which are endemic to the Amazon region. These fishes are the smallest known South American vertebrates and among the smallest know vertebrates on Earth. All species but the type F. pygmaeus have been described in late 1990’s, and much remains unknown about the biology, taxonomy and systematics of this group of fishes. The aims of the present study were to establish the diploid and haploid number of F. zonatus and F. simplex, and to find species-specific markers for the discrimination of taxa. The diploid number for both species was 48 chromosomes, with no sex chromosome heteromorphism. Fluviphylax zonatus exhibited the karyotypic formula 4m+8sm+22st+14a and FN=82, and F. simplex exhibited 4m+16sm+18st+10a and FN=86. The determination of the total mean length of the chromosomes and their grouping into five size classes demonstrated different chromosome composition of the two species. This difference was further supported by the distribution of constitutive heterochromatin. The meiotic analysis revealed 24 bivalents in both species, but F. zonatus exhibited chromosomes with late pairing of the telomeric portions in the pachytene. These data reveal that cytogenetic characterization is useful and important for the discrimination of these species. Our study further indicates that this method could be employed in the analysis of other species of small fishes that are difficult to distinguish using traditional morphological traits or are morphologically cryptic. text/html en_US Pensoft Publishers Chromosomes heterochromatin killifish meiosis mitosis Comparative cytogenetics of two of the smallest Amazonian fishes: Fluviphylax simplex Costa, 1996 and Fluviphylax zonatus Costa, 1996 (Cyprinodontiformes, Poeciliidae) Research Article

10.3897/compcytogen.v5i5.1252 2011-12-22 compcytogen

Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow, author Lavrenchenko, Leonid Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow, Russia author Nadjafova, Rena Institute of Ecology and Evolution, Moscow, Moscow, Russia author Bulatova, Nina https://orcid.org/0000-0003-1225-2195 2011-12-22 2011-12-22 2011 Comparative Cytogenetics 1993-078X 1993-0771 5 423 431 2011 P-O Barome author 2001 Phylogeny of Acomys spinosissimus (Rodentia, Muridae) from north Malawi and Tanzania: evidence from morphological and molecular analysis. Biological Journal of the Linnean Society 73: 321–340. http://www.idealibrary.com 2001 N Bulatova author 2009 Field protocols for the genomic era. Comparative Cytogenetics 3(1): 57–62. http://www.zin.ru/journals/compcyt/abstract.asp?k=54 2009 M Corti author 2005 Cytotaxonomy of rodent species from Ethiopia, Kenya, Tanzania and Zambia. Belgian Journal of Zoology 135 (supplement): 197–216. 2005 Rodents from Adrar des Iforas, Mali. Chromosomal data. G Dobigny author 2001 text Mammalia 2001 65 215 220 A cytotaxonomic survey of Rodents from Niger: implications for systematics, biodiversity and biogeography. G Dobigny author 2002 text Mammalia 2002 66 495 523 The Rodent fauna of Tanzania: a cytotaxonomic report from the Maasai Steppe (1999). C Fadda author 2001 text Rendiconti Lincei 2001 9 12 29 49 A colchicine, hypotonic citrate squash sequence for mammalian chromosomes. CE Ford author 1956 text Stain Technology 1956 31 247 251 New data on the evolution of the Cretan spiny mouse, Acomys minous (Rodentia: Murinae), shed light on the phylogenetic relationships in the cahirinus group. EB Giagia-Athanasopoulou author 2011 text Biological Journal of the Linnean Society 2011 102 498 509 The importance of cytotaxonomy in understanding the biogeography of African rodents: Lake Chad murids as an example. L Granjon author 2003 text Mammal Review 2003 33 77 91 LA Lavrenchenko author 2009 The mammals of the Ethiopian Plateau: general trends and peculiarities in forming of a tropical montane fauna. Dr. Sci. Dissertation, Moscow. Russian Federation: Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences. 302 pp. 2009 Karyotype of Acomys cilicicus Spitzenberger, 1978 (Rodentia, Muridae). M Macholán author 1995 text Mammalia 1995 59 3 397 402 La formule chromosomique de quelques Murinae (Muridae - Rodentia - Mammalia). R Matthey author 1956 text Archiv der Julius Klaus-Sitftung für Vererbungsforschung 1956 31 294 306 Cytogénétique et taxonomie du genre Acomys: A. percivali Dollmann et A. wilsoni Thomas, espèces d’Abyssinie. R Matthey author 1968 text Mammalia 1968 32 621 627 Phylogeography of spiny mice (genus Acomys, Rodentia: Muridae) from the south-western margin of the Sahara with taxonomic implications. V Nicolas author 2009 text Biological Journal of the Linnean Society 2009 98 29 46 A karyological study of the spiny mouse Acomys Geoffroy 1838 (Rodentia, Muridae) along the Ethiopian Rift Valley. VE Sokolov author 1993 text Tropical Zoology 1993 6 227 235 Karyotypes of seven rodents from Jordan (Mammalia: Rodentia). M Sözen author 2008 text Zoology in the Middle East 2008 44 3 10 Species delimitation in the Acomys cahirinus–dimidiatus complex (Rodentia, Muridae) inferred from chromosomal and morphological analyses. V Volobouev author 2007 text Biological Journal of the Linnean Society 2007 91 203 214 Chromosome evolution in the genus Acomys (Rodentia, Muridae): chromosome banding analysis in Acomys cahirinus. V Volobouev author 1996 text Mammalia 1996 60 217 222 Chromosome evolution in the genus Acomys: chromosome banding analysis of Acomys cf. dimidiatus (Rodentia, Muridae). V Volobouev author 1991 text Bonner zoologischer Beiträge 1991 42 3–4 253 260 Hybridization in nature between two chromosome forms of spiny mice. J Wahrman author 1972 text Chromosomes Today 1972 3 228 237 J Wahrman author 1953 Intra-generic difference in chromosome numbers of spiny mice (Rodentia: Murinae). Bulletin of Research Council of Israel 3: 265. 1953 Chromosomal banding pattern in the Cyprus spiny mouse, Acomys nesiotes. J Zima author 1999 text Folia Zoologica 1999 48 149 152 10.3897/compcytogen.v5i5.1252 https://compcytogen.pensoft.net/article/1737/ https://compcytogen.pensoft.net/article/1737/download/pdf/ https://compcytogen.pensoft.net/article/1737/download/xml/ Three new karyotypes (2n=40, 44, 52) are described revealing what are probably new cryptic species of Ethiopian spiny mice. Two other diploid numbers have already been reported for the country (2n=36 and 68) and, overall, the five known karyotypic forms constitute a common lineage differentiated by a Robertsonian process. Such arrays of karyotypic forms are known as a ‘Robertsonian fan’. This view of the situation in Ethiopian Acomys I. Geoffroy, 1838 is based on standard chromosomal morphology that reveals a constant FN (68) and needs further investigation of chromosome homology by differential staining and/or molecular cytogenetic techniques as well as further molecular phylogenetic analysis. text/html en_US Pensoft Publishers mammalian karyotype Robertsonian variation Acomys Three new karyotypes extend a Robertsonian fan in Ethiopian spiny mice of the genus Acomys I. Geoffroy, 1838 (Mammalia, Rodentia) Research Article

10.3897/compcytogen.v5i5.1720 2011-12-22 compcytogen

A.N. Severtsov Institute of Ecology and Evolution, Moscow, author Pavlova, Svetlana V. https://orcid.org/0000-0001-7823-9618 Russian Academy of Sciences, Moscow, author Tchabovsky, A.V. 2011-12-22 2011-12-22 2011 Comparative Cytogenetics 1993-078X 1993-0771 5 433 440 2011 About the nature of the sand-flows on Olkhon. Izvestiya RAN. BP Agafonov author 2006 text Seriya geograficheskaya 2006 5 101 108 MI Baskevich author 2008 New data on chromosomal variability and distribution of sibling species Microtus arvalis sensu lato (Rodentia, Arvicolinae) in Lower Volga Region. Zoologicheskii Zhurnal 87(11): 1382-1390. [In Russian] 2008 Pattern of X-Y chromosome pairing in microtine rodents. PM Borodin author 1995 text Hereditas 1995 123 17 23 Field protocols for the genomic era. NSh Bulatova author 2009 text CompCytogen 2009 3 1 57 62 Common Vole: The sibling species author 1994 Microtus arvalis Pallas, 1779, M. rossiaemeridionalis Ognev, 1924. Nauka Publishing House, Moscow, 429 pp. 1994 A colchicine, hypotonic citrate, squash sequence for mammalian chromosomes. CE Ford author 1956 text Stain Technology 1956 31 247 251 The ‘common vole’ in Svalbard identified as Microtus epiroticus by chromosome analysis. K Fredga author 1990 text Polar Research 1990 8 283 290 Geological progress of Baikal rift. GI Galazij author NS Ovchinnikova author 2005 text Irkutsk 2005 16 20 IV Kartavtseva author 2011 New species of common vole in territory of Far East of Russia. Abstracts of International Meeting “Theriofauna of Russia and adjacent territories”, 1–4 February 2011, Moscow, Russia. Moscow, 2011 2011 On the distribution of voles of the group arvalis (Rodentia) in Kazakhstan. YuM Kovalskaya author 1994 text Zoologicheskii Zhurnal 1994 73 3 120 125 Distribution of Microtus arvalis and M. epiroticus. B Král author 1980 text Acta Scientiarum Naturalia Brno 1980 14 9 1 31 B Král author 1975  Karyotypes of 46-chromosome Microtus arvalis (Microtidae, Rodentia). Zoologičke Listy 24(1): 1–11. 1975 VM Malygin author 1983 Systematics of the common vole. Nauka. Moscow, 208. 1983 Taxonomic nomenclature of sibling species of the common vole (Rodentia, Cricetidae). VM Malygin author 1986 text Zoologicheskii Zhurnal 1986 65 4 579 591 High-resolution G-banding of chromosomes in Microtus subarvalis (Rodentia, Arvicolidae). NA Mazurok author 1995 text  Hereditas 1995 123 47 52 Utilization of karyological, physiological and cytological analysis for the separation of new species of rodents (Rodentia, Mammalia). MN Meyer author 1969 text Doklady AN SSSR 1969 188 1411 1414 Sibling species in the group Microtus arvalis (Rodentia, Cricetidae). MN Meyer author 1972 text Zoologicheskii Zhurnal 1972 51 724 737 MN Meyer author 1996 Voles (subgenus Microtus Schrank) of Russia and adjacent territories. Sankt-Petersburg, 320 pp. 1996 Heterochromatin study and geographical distribution of Microtus species (Rodentia, Arvicolinae) from Greece. GP Mitsainas author 2010 text Mammalian Biological 2010 75 261 269 GI Shenbrot author 2005 Atlas of the Geographic Distribution of the Arvicoline Rodents of the World (Rodentia, Muridae: Arvicolinae). Pensoft Publishers, Sofia. 2005 A simple technique for demonstrating centromeric heterochromatin. AT Sumner author 1972 text Experimental Cell Research 1972 75 304 306 Ecological characterization of background rodent species in steppes of the lower reaches of the Volga and Don rivers. GI Tikhonova author 2005 text Povolzhskiy journal of ecology 2005 3 281 29 On karyotype variation in common vole Microtus rossiaemeridionalis (Rodentia, Cricetidae). LV Yakimenko author 1997 text Zoologicheskii Zhurnal 1997 76 3 375 378 A method for the differentiation of male meiotic chromosome stages. D Williams author 1971 text Journal of Heredity 1971 62 17 22 10.3897/compcytogen.v5i5.1720 https://compcytogen.pensoft.net/article/1736/ https://compcytogen.pensoft.net/article/1736/download/pdf/ https://compcytogen.pensoft.net/article/1736/download/xml/ We report a new finding of the 54-chromosome sibling species of the common vole in East Siberia - the first description from Olkhon Island (Lake Baikal). The karyotype of a male specimen revealed by routine staining and C-banding demonstrates the unambiguous presence of Microtus rossiaemeridionalis Ognev, 1924 (recently often regarded as as junior synonym of M. levis Miller, 1908). Comparison with conspecific specimens from the European part of the species range (from the left bank of the river Volga) shows that the vole of the island population has a smaller X-chromosome due to a reduced quantity of C-positive heterochromatin. This is just the third example of this type of X-chromosome variant with previous cases on an Arctic island (Svalbard) and the West Siberian lowland (Novosibirsk) and the only one on a lake island. Although M. rossiaemeridionalis is largely monomorphic in its karyotype, our data show that one specific type of X-chromosome variant is remarkably widespread, though rare. text/html en_US Pensoft Publishers chromosome sibling species common voles Microtus arvalis group Microtus rossiaemeridionalis Lake Baikal X-chromosome Presence of the 54-chromosome common vole (Mammalia) on Olkhon Island (Lake Baikal, East Siberia, Russia), and the occurrence of an unusual X-chromosome variant Research Article

10.3897/compcytogen.v6i1.1752 2012-01-24 compcytogen

Universidade Estadual de Maringá, Brazil author Mendes, Valéria Universidade Estadual de Maringá, Maringá, Brazil author Portela-Castro, Ana Luiza https://orcid.org/0000-0002-0881-0345 Universidade Estadual de Maringá, Brazil author Júlio-Júnior, Horácio 2012-01-24 2012-01-24 2012 Comparative Cytogenetics 1993-078X 1993-0771 6 1 1 16 2012 Karyotype and B chromosome of Rahmdia species (Pisces, Pimelodidae) endemic in the River Iguaçu basin. M Abucarma author 2001 text Cytologia 2001 66 299 306 LF Almeida-Toledo author 1978 Contribuição à Citogenética dos Gymnotoidae (Pisces, Ostariophysis). Doctoral Thesis, Instituto de Biociências, Universidade de São Paulo, São Paulo. 128pp. 1978 Constitutive heterochromatin and nucleolus organizer region in the knifefish, Apteronotus albifrons (Pisces, Apteronotidae). LF Almeida-Toledo author 1981 text Experientia 1981 37 953 954 Fluorescence in situ hybridization with rDNA probes on chromosomes of two nucleolus organizer phenotypes of a species of Eigenmannia (Pisces, Gymnotoidei, Sternopygidae). LF Almeida-Toledo author 1996 text Chromosome Research 1996 4 301 305 LF Almeida-Toledo author 2000a Sex chromosome evolution in fish: the formation of the neo-Y chromosome in Eigenmannia (Gymnotiformes) Chromosoma 109: 197–200. 2000a Sex chromosome evolution in fish: second occurrence of a X1X2Y sex chromosome system in Gymnotiformes. LF Almeida-Toledo author 2000b text Chromosome Research 2000b 8 335 340 XX:XY sex chromosomes system with X heterochromatinization; an early stage of sex chromosome differentiation in the Neotropic eletric eel Eigenmannia virescens. LF Almeida-Toledo author 2001 text Cytogenetics and Cell Genetics 2001 95 73 78 Chromosome variability in Gymnotiformes (Teleostei: Ostariophysi). LF Almeida-Toledo author E Pisano author 2007 text Science Publishers, Enfield 2007 17 39 R Arai author 2011 Fish karyotypes: a check list. 2011 Cytotaxonomic consideration on Hoplias lacerdae (Pisces, Erythrinidae). LAC Bertollo author 1978 text Brazilian Journal of Genetics 1978 1 2 103 120 B-chromosome evolution. JPM Camacho author 2000 text Philosophical Transactions of the Royal Society (B) 2000 55 163 178 Chromosomal characterization of two species of genus Steatogenys (Gymnotiformes: Rhamphichthyoidea: Steatogenini) from the Amazon bas AL Cardoso author phylogeny sex chromosomes and correlations with Gymnotiformes author 2011 text 2011 21 613 621 B chromosomes: an update about their occurrence in freshwater Neotropical fishes (Teleostei). RA Carvalho author 2008 text Journal of Fish Biology 2008 72 1907 1932 An ecological perspective on diversity and distribuitions. WRG Crampton author JS Albert author 2011 text University of California Press, Berkeley 2011 65 189 Mapping and organization of highly-repeated DNA sequences by means of simultaneous and sequential FISH and C-banding in 6x-triticale. AE Cuadrado author 1994 text Chromosome Research 1994 2 331 338 Possible origins of B chromosomes in Rineloricaria pentamaculata (Loricariidae, Siluriformes) from the Paraná River basin. F Errero-Porto author 2010 text Genetics and Molecular Research 2010 9 3 1654 1659 Supernumerary chromosomes in Rhamdia hilarii population (Pisces, Pimelodiadae). AS Fenocchio author 1990 text Genetica 1990 81 193 1998 Cytogenetic characterization of the strongly electric Amazonian eel, Electrophorus electricus (Teleostei, Gymnotiformes), from the Brazilian rivers Amazon and Araguaia. SB Fonteles author 2008 text Genetics and Molecular Biology 2008 31 227 230 Polymorphic nature of nucleolus organizer regions in fishes. F Foresti author 1981 text Cytogenetics and Cell Genetics 1981 31 137 144 Chromosome studies in Gymnotus carapo and Gymnotus sp. (Pisces, Gymnotidae). F Foresti author 1984 text Caryologia 1984 37 141 146 F Foresti author 1987 Estudos cromossômicos em Gymnotiformes (Pisces, Ostariophysis). 1987 Supernumerary chromosome system, C-banding pattern characterization and multiple nucleolus organizer regions in Moenkhausia sanctaefilomenae (Pisces, Characidae). F Foresti author 1989 text Genetica 1989 79 107 114 Brachyhypopomus gauderio,new species, a new example of underestimated species diversity of electric fishes in the southern South America (Gymnotiformes: Hypopomidae). J Giora author 2009 text Zootaxa 2009 2093 60 68 . In situ hybridization with automated chromosome denaturation. J Heslop-Harrison author 1991 text Technique 1991 3 109 116 Cellular DNA content and the evolution of teleostean fishes. R Hinegardner author 1972 text The AmericanNaturalist 1972 106 621 644 Somatic chromosomes of the black ghost knifefish Apteronotus albifrons (Pisces, Apteronotidae). WM Howell author 1972 text Copeia 1972 1 191 193 WM Howell author 1980 text Experientia 1980 36 1014 1015 Nomenclature for centromeric position on chromosomes. A Levan author 1964 text Hereditas 1964 52 201 220 Differences in karyotype between two sympatric species of Gymnotus (Gymnotiformes: Gymnotidae) from the eastern Amazon of Brazil. SSR Milhomem author 2007 text Zootaxa 2007 1397 55 62 B chromosomes in four different populations of Rhamdia quelen (Siluriformes, Heptapteridae); a comparative study of frequency and distribution. VPO Moraes author 2009 text Folia Biologica (Kraków) 2009 57 165 169 B chromosomes in the fish Astyanax scabripinnis (Characidae, Tetragonopterinae): an overview in natural populations. O Moreira-Filho author 2004 text Cytogenetics and Genome Research 2004 106 230 234 Occurrence of supernumerary microchromosomes in Steindachnerina insculpta (Pisces, Characiformes, Curimatidae). C Oliveira author 1993 text Cytobios 1993 76 183 186 Genetics of Neotropical fish: from chromosomes to populations. C Oliveira author 2009 text Fish Physiology and Biochemistry 2009 35 81 100 New occurrence of microchromosomes B in Moenkhausia sanctaefilomenae (Pisces, Characidae) from the Paraná River of Brazil: analysis of the synaptonemal complex. ALB Portela-Castro author 2001 text Genetica 2001 110 277 283 Apteronutus caudimaculosus sp. n. (Gymnotiformes: Apteronotidae), a sexually dimorphic black ghost knifefish from the Pantanal, Western Brazil, with a note on the monophyly of the A. albifrons species complex. CD Santana author 2003 text Zootaxa 2003 252 1 11 DS Silva author 2009 Cytogenetic studies in Eigenmannia virescens (Sternopygidae, Gymnotiformes) and new inferences on the origin of sex chromosomes in the Eigenmannia genus. BMC Genetics 10: 74. 2009 An X1X1X2X2/X1X2Y multiple sex chromosome system in a new species of the genus Gymnotus (Pisces, Gymnotiformes). EB Silva author 2005 text Environmental Biology of Fishes 2005 73 293 297 PC Silva author 2010 Estudos cromossômicos de peixes elétricos da família Rhamphichthydae (Pisces: Gymnotiformes). Master Dissertation, Universidade Federal do Pará, Belém, Brazil. 2010 A simple technique for demonstrating centromeric heterochromatin. AT Sumner author 1972 text Experimental Cell Research 1972 75 304 306 Heteromorphism of rDNA size in Pinirampus pirinampu (Pisces, Pimelodidae) detected in situ hybridization. AC Swarça author 2001 text Cytologia 2001 66 275 278 New cases of supernumerary chromosomes in Characiform fishes. PC Venere author 1999 text Genetics and Molecular Biology 1999 22 345 349 10.3897/compcytogen.v6i1.1752 https://compcytogen.pensoft.net/article/1748/ https://compcytogen.pensoft.net/article/1748/download/pdf/ https://compcytogen.pensoft.net/article/1748/download/xml/ Cytogenetic studies were performed on the species Apteronotus prope albifrons Linnaeus, 1766, Rhamphichthys hahni Meinken, 1937 and Brachyhypopomus gauderio Giora & Malabarba, 2009, collected in the upper Paraná River floodplain, Porto Rico (PR), Brazil. Apteronotus prope albifrons showed a diploid number of 2n=24 chromosomes for both sexes and a karyotype formula of 14m+2sm+2st+6a (FN=42). Besides the standard karyotype, three specimens had one to three extra microchromosomes with inter- and intra-individual variations, which suggested the occurrence of B chromosomes in the species. The chromosomal data of R. hahni, described here for the first time, consists of 50 chromosomes and a formula comprised of 20m+24sm+6a (FN=94). Brachyhypopomus gauderio specimens demonstrated 2n=42 chromosomes in females, all acrocentric, and 2n=41 chromosomes in males, with 40 acrocentric and 1 medium-sized metacentric chromosome. These differences concern with a multiple system of sex chromosome determination X1X1X2X2/X1X2Y (FN=42) in B. gauderio. The analysis of nucleolar organizer regions by Ag-NOR and FISH 18S banding revealed a simple NOR system in A. prope albifrons and R. hahni and a multiple NOR system in B. gauderio, that is unusual for Gymnotiformes fishes. Constitutive heterochromatin was mainly found in the pericentromere region in most of the chromosomes of the three species, although each species had its own peculiarities. The B chromosomes in Apteronotus prope albifrons demonstrated heterochromatin positioned in the centromeric and telomeric regions whereas R. hahni presented conspicuous blocks of heterochromatin on the long arms in three submetacentric pairs. Brachyhypopomus gauderio showed blocks of heterochromatin on the long arm in the interstitial and telomere positions. The finding of B chromosomes in A. prope albifrons represents the first description of these elements in the Gymnotiformes order. Although the karyotype of this species is similar with that described for populations in the Amazon basin, the presence of B chromosomes could represent a specific characteristic of this population. A comparative analysis of karyotypes of R. hahni with other species of the genus showed a relatively conservative structure suggesting 2n=50 as a common number in this group. The karyotype of B. gauderio, a new species, provides an important reference for future chromosome studies of the Brachyhypopmus Mago-Lecia, 1994, and it might be also significant for cytotaxonomy in this group. The cytogenetic data also demonstrate the need of more comparative cytogenetic studies in the families of the highly diversified and taxonomically difficult complex Gymnotiformes. text/html en_US Pensoft Publishers fish cytogenetics B chromosomes C-banding ribosomal DNA sex chromosomes First record of supernumerary (B) chromosomes in electric fish (Gymnotiformes) and the karyotype structure of three species of the same order from the upper Paraná River basin Research Article

10.3897/compcytogen.v6i1.1510 2012-01-24 compcytogen

Universidade Federal de Pernambuco, Recife, Brazil author de Lemos Pinto, Marcela Universidade Federal de Pernambuco, Recife, Brazil author da Silva Calixto, Merilane Universidade Federal de Pernambuco, Recife, Brazil author de Souza, Maria José Universidade Federal da Paraíba, João Pessoa, Brazil author de Araújo, Ana Paloma Tavares Universidade Federal da Paraíba, João Pessoa, Brazil author Langguth, Alfredo UFPE, Recife, Brazil author Santos, Neide 2012-01-24 2012-01-24 2012 Comparative Cytogenetics 1993-078X 1993-0771 6 1 17 28 2012 For morphometric analysis, forearm and cranial measurements were used to express the morphological differences between the species studied. P Araújo author 2010 text Chiroptera Neotropical 2010 16 2 715 722 Karyotypic evolution in bats: evidence of extensive and conservative chromosomal evolution in closely related taxa. RJ Baker author 1980 text Systematic Zoology 1980 29 239 253 Further studies on the sex-chromosome systems of the American leaf-nosed bats (Chiroptera: Phyllostomatidae). RJ Baker author 1970 text Cytogenetics 1970 9 131 138 Diversification among New World leaf-nosed bats: An evolutionary hypothesis and classification inferred from digenomic congruence of DNA sequence. RJ Baker author 2003 text Occasional Paper of the Museum of Texas Tech University 2003 230 1 32 Comparative cytogenetic analysis between Lonchorhina aurita and Trachops cirrhosus (Chiroptera: Phyllostomidae). HMDR Barros author 2009 text Genetics and Molecular Biology 2009 32 748 752 Cis-acting regulation of NOR cistrons in Eleutherine bulbosa (Iridaceae). M Dos Santos Guerra author 1991 text Genetica 1991 83 235 241 Karyotypic composition of bats from the Brazilian nuclear power plant, state of Rio de Janeiro. JP Garcia author 2010 text Chiroptera Neotropical 2010 16 1 617 628 Chromosomal Variation in Vampyressa and a Review of Chromosomal Evolution in the Phyllostomidae (Chiroptera). AL Gardner author 1977 text Systematic Zoology 1977 26 3 300 318 L Granjon author 2003 Chromosomally characterised murid rodents from the edges of Lake Chad: evidence for an African biogeographical crossroads, or a centre of endemism? Mammalian Review 33: 77–91. 2003 How did the platypus get its sex chromosome chain? A comparison of meiotic multiples and sex chromosomes in plants and animals. F Gruetzner author 2006 text Chromosoma 2006 115 75 88 Morphometrics in the quantification of character state identity for the assessment of primary homology: an analysis of character variation of the genus Artibeus (Chiroptera: Phyllostomidae). JA Guerrero author 2003 text Biological Journal of the Linnean Society 2003 80 45 55 Jr CO Handley author 1989 The Artibeus of Gray 1838. 1989 Mammalian species: Artibeus obscurus. MA Haynes author 2004 text American Society of Mammalogists 2004 752 1 5 Mammalian species: Artibeus planirostris. L Hollis author 2005 text American Society of Mammalogists 2005 775 1 6 Phylogenetics of the fruit-eating bats (Phyllostomidae: Artibeina) inferred from mitochondrial DNA sequences. SR Hoofer author 2008 text Occasional Paper of the Museum of Texas Tech University 2008 277 1 15 Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. WM Howell author 1980 text Experientia 1980 36 1014 1015 The multiple sex chromosome system of American leaf-nosed bats (Chiroptera, Phyllostomatidae). T Hsu author 1968 text Cytogenetics 1968 7 27 38 Taxonomic status of Andersen’s fruit-eating bat (Artibeus jamaicensis aequatorialis) and revised classification of Artibeus (Chiroptera: Phyllostomidae). PA Larsen author 2010 text Zootaxa 2010 2648 45 60 Karyotypic characterization of the bat species Molossus ater, M. molossus and Molossops planirostris (Chiroptera, Molossidae) using FISH and banding techniques. C Leite-Silva author 2003 text Hereditas 2003 138 94 100 Cranial differentiation of fruit-eating bats (genus Artibeus) based on size-standardized data. MR Marchán-Rivadeneira author 2010 text Acta Chiropterologica 2010 12 1 143 154 Variability of nucleolus organizer regions in phyllostomid bats. E Morielle author 1988 text Revista Brasileira de Genética 1988 11 853 871 Neo-XY body: an analysis of XY1Y2 meiotic behavior in Carollia (Chiroptera, Phyllostomidae) by chromosome painting. RR Noronha author 2009 text Cytogenetic and Genome Research 2009 124 1 37 43 Meiotic analyses of the sex chromosomes in Carolliinae-Phyllostomidae (Chiroptera): NOR separates the XY1Y2 into two independent parts. RR Noronha author 2004 text Caryologia 2004 57 1 1 9 Phylogenetic analyses of the bat subfamily Stenodermatinae (Mammalia: Chiroptera). RD Owen author 1987 text Special Publications of the Museum of Texas Tech University 1987 26 1 65 The systematic status of Dermanura concolor (Peters, 1865) (Chiroptera: Phyllostomidae) with description of a new genus. RD Owen author 1991 text Bulletin American Museum of Natural History 1991 206 18 25 Chromosome comparison between two species of Phyllostomus (Chiroptera – Phyllostomidae) from Eastern Amazonia, with some phylogenetic insights. LRR Rodrigues author 2000 text Genetics and Molecular Biology 2000 23 3 595 599 Comparative cytogenetics of two phyllostomids bats. A new hypothesis to the origin of the rearranged X chromosome from Artibeus lituratus (Chiroptera,Phyllostomidae). LRR Rodrigues author 2003 text Caryologia 2003 56 4 413 419 Characterization of the constitutive heterochromatin of Carollia perspicillata (Phyllostomidae, Chiroptera) using the base-specific fluorochromes, CMA3 (GC) and DAPI (AT). N Santos author 1998a text Caryologia 1998a 51 51 60 Use of fluorochromes chromomycin A3 and DAPI to study constitutive heterochromatin and NORs in four species of bats (Phyllostomidae). N Santos author 1998b text Caryologia 1998b 51 265 278 Comparative karyology of brazilian vampire bats Desmodus rotundus and Diphylla ecaudata (Phyllostomidae, Chiroptera): banding patterns, base-specific fluorochromes and FISH of ribossomal genes. N Santos author 2001 text Hereditas 2001 134 189 194 Localization of rRNA genes in Phyllostomidae bats revels silent NORs in Artibeus cinereus. N Santos author 2002 text Hereditas 2002 136 137 143 The karyotype of Macrophyllum macrophyllum (Schinz, 1821) (Phyllostomidae: Phyllostominae) from the state of Bahia, Brazil. IA Sbragia author 2010 text Chiroptera Neotropical 2010 16 1 600 602 Comparative cytogenetic analysis in the species Uroderma magnirostrum and U. bilobatum (cytotype 2n=42) (Phyllostomidae, Stenodermatinae) in the Brazilian Amazon. AM Silva author 2005 text Genetics and Molecular Biology 2005 28 248 253 NB Simmons author 2005 Mammal species of the world: A Taxonomic and geographic reference. Baltimore, The Johns Hopkins University Press, 3ªed (1): 312–529. 2005 Conservative pattern of the G-bands and diversity of C-banding patterns and NORs in Stenodermatinae (Chiroptera-Phyllostomatidae). MJ Souza author 1990 text Revista Brasileira de Genética 1990 13 255 268 A simple technique for demonstrating centromeric heterochromatin. AT Sumner author 1972 text Experimental Cell Research 1972 75 304 306 Distribuição geográfica e análise morfométrica comparativa em Artibeus obscurus (Schinz, 1821) e Artibeus fimbriatus Gray, 1838 (Mammalia, Chiroptera, Phyllostomidae). VA Taddei author 1998 text Ensaios e ciência 1998 2 71 127 RA Van Den Bussche author 1998 Phylogenetic accuracy, stability, and congruence: relationships within and among the New World bat genera Artibeus, Dermanura, and Koopmania, (59–71). In: T. H. Kunz and P. A. Racey (Eds) Bat biology and conservation. Smithsonian Institution Press, Washington, D.C. 365pp. 1998 A survey of cytogenetic data on Brazilian bats. M Varella-Garcia author 1989 text Revista Brasileira de Genética 1989 12 761 793 Phylogeny of phyllostomid bats (Mammalia: Chiroptera): Data from diverse morphological systems, sex chromosomes, and restriction sites. AL Wetterer author 2000 text Bulletin American Museum of Natural History 2000 248 1 200 10.3897/compcytogen.v6i1.1510 https://compcytogen.pensoft.net/article/1747/ https://compcytogen.pensoft.net/article/1747/download/pdf/ https://compcytogen.pensoft.net/article/1747/download/xml/ The genus Artibeus represents a highly diverse group of bats from the Neotropical region, with four large species occurring in Brazil. In this paper, a comparative cytogenetic study was carried out on the species Artibeus obscurus Schinz, 1821, A. fimbriatus Gray, 1838, A. lituratus Olfers, 1818 and A. planirostris Spix, 1823 that live sympatrically in the northeast of Brazil, through C-banding, silver staining and DNA-specific fluorochromes (CMA3 and DAPI). All the species had karyotypes with 2n=30,XX and 2n=31,XY1Y2, and FN=56. C-banding showed constitutive heterochromatin (CH) blocks in the pericentromeric regions of all the chromosomes and small CH blocks at the terminal region of pairs 5, 6, and 7 for all species. Notably, our C-banding data revealed species-specific autosomic CH blocks for each taxon, as well as different heterochromatic constitution of Y2 chromosomes of A. planirostris. Ag-NORs were observed in the short arms of chromosomes 5, 6 and 7 in all species. The sequential staining AgNO3/CMA3/DA/DAPI indicated a positive association of CH with Ag-NORs and positive CMA3 signals, thus reflecting GC-richness in these regions in A. obscurus and A. fimbriatus. In this work it was possible to identify interespecific divergences in the Brazilian large Artibeus species using C-banding it was possible provided a suitable tool in the cytotaxonomic differentiation of this genus. text/html en_US Pensoft Publishers C-banding Ag-NOR CMA3/DA/DAPI Cytotaxonomy Cytotaxonomy of the subgenus Artibeus (Phyllostomidae, Chiroptera) by characterization of species-specific markers Research Article

10.3897/compcytogen.v6i1.2151 2012-01-24 compcytogen

UNESP, Botucatu, Brazil author Ferreira-Neto, Maressa UEPG, Ponta Grossa, Brazil author Artoni, Roberto Universidade Estadual de Ponta Grossa, Ponta Grossa, Brazil author Vicari, Marcelo https://orcid.org/0000-0003-3913-9889 Universidade Federal de São Carlos, Brazil author Moreira-Filho, Orlando Universidad de Granada, Granada, Spain author Camacho, Juan Pedro M. Universidad de Granada, Granada, Spain author Bakkali, Mohammed Universidade Estadual Paulista, Botucatu, Brazil author Oliveira, Claudio Universidade Estadual Paulista (UNESP), Botucatu, Brazil author Foresti, Fausto https://orcid.org/0000-0002-0862-0445 2012-01-24 2012-01-24 2012 Comparative Cytogenetics 1993-078X 1993-0771 6 1 29 40 2012 Astyanax aff. fasciatus Cuvier, 1819 (Teleostei; Characidae): evidences of a species complex in the upper rio Tibagi basin (Paraná, Brazil). RF Artoni author 2006 text Neotropical Ichthyology 2006 4 197 202 M Bakkali author 2001 text Protoplasma 2001 217 185 190 Karyotypic diversity and distribution in Hoplias malabaricus (Pisces, Erythrinidae): Cytotypes with 2n = 40 chromosomes. LAC Bertollo author 1997 text Brazilian Journal of Genetics 1997 20 237 242 Geographical distribution of B chromosomes in the grasshopper Eyprepocnemis plorans along a river basin, is mainly shaped by non-selective historical events. J Cabrero author 1997 text Chromosome Research 1997 5 194 198 Location and expression of ribosomal RNA genes in grasshoppers: Abundance of silent and cryptic loci. J Cabrero author 2008 text Chromosome Research 2008 16 595 607 B chromosomes. JPM Camacho author TR Gregory author 2005 text Elsevier, San Diego 2005 223 286 Chromosomal differentiation among allopatric populations of Hyphessobrycon anisitsi (Pisces, Tetragonopterinae). L Centofante author 2003 text Cytology 2003 68 283 288 Chromosomal evidence for a putative cryptic species in the Gymnotus carapo species-complex (Gymnotiformes, Gymnotidae). DS Crampton author 2008 text BMC Genetics 2008 9 75 85 “Ag-NORs” are not always true NORs: New evidence in mammals. G Dobigny author 2002 text Cytogenetic and Genome Research 2002 98 75 77 Natural triploidy and B chromosomes in the neotropical fish genus Astyanax (Characidae). G Fauaz author 1994 text Brazilian Journal of Genetics 1994 17 157 163 Nucleolar organizing regions, 18S and 5S rDNA in Astyanax scabripinnis (Pisces, Characidae): Populations distribution and functional diversity. DAM Ferro author 2001 text Genetica 2001 110 55 62 Polymorphic nature of nucleolus organizer regions in fishes. F Foresti author 1981 text Cytogenetics and Cell Genetics 1981 31 137 144 Mapping of the 18S and 5S ribosomal rDNA genes in the fish Prochilodus argenteus, Agassiz, 1829 (Characiformes, Prochilodontidae). T Hatanaka author 2004 text Genetica 2004 122 239 244 Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. WM Howell author 1980 text Experientia 1980 36 1014 1015 Distribution of 18S-28S ribosomal genes in mammalian genomes. TC Hsu author 1975 text Chromosome 1975 53 25 36 Molecular cloning and characterization of the germline-restricted chromosome sequence in the zebra finch. Y Itoh author 2009 text Chromosoma 2009 118 527 536 Chromosomal location of 5S and 18S rRNA genes in Prochilodus lineatus (Characiformes, Prochilodontidae). CM Jesus author 2003 text Caryologia 2003 56 281 287 Nomenclature for centromeric position of chromosomes. A Levan author 1964 text Hereditas 1964 52 201 220 Occurrence of macro B chromosomes in Astyanax scabripinnis paranae (Pisces, Characiformes, Characidae). EL Maistro author 1992 text Genetica 1992 87 101 106 Chromosomal localization of 5S rDNA genes in Leporinus fish (Anostomidae, Characiformes). C Martins author 1999 text Chromosome Research 1999 7 363 367 Structural and functional evidence that a B chromosome in the characid fish Astyanax scabripinnis is an isochromosome. CA Mestriner author 2000 text Heredity 2000 85 1 9 SSR Milhomem author 2007 The callichthyid catfish, Corydoras aeneus is an unresolved diploid-tetraploid sibling species complex. Ichthyological Exploration of Freshwaters 3: 17. 2007 Macro and microchromosomes B in females of Astyanax scabripinnis (Pisces, Characidae). SMHN Mizoguchi author 1997 text Heredity 1997 127 249 253 Astyanax scabripinnis (Pisces, Characidae): A species complex. O Moreira-Filho author 1991 text Genetics and Molecular Biology 1991 14 331 357 Occurrence of a metacentric macrochromosome B in different species of the genus Astyanax (Pisces, Characidae, Tetragonopterinae). O Moreira-Filho author 2001 text Cytologia 2001 66 59 64 B chromosomes in the fish Astyanax scabripinnis (Characidae, Tetragonopterinae): an overview in natural populations. O Moreira-Filho author 2004 text Cytogenetic and Genome Research 2004 106 230 234 Cytogenetic considerations on the genus Astyanax (Pisces, Characidae) I Karyotypic variability. S Morelli author 1983 text Caryologia 1983 36 235 244 Chromosome polymorfism in Astyanax fasciatus (Teleostei, Characidae) 1. Karyotype analysis, Ag-NORs and mapping of 18S and 5S ribossomal genes in sympatric Karyotypes and their possible hybrid forms. R Pazza author 2006 text Cytogenetic and Genome Research 2006 112 313 319 The species complex Astyanax fasciatus Cuvier, 1819 (Teleostei, Characiformes): A multidisciplinary approach. R Pazza author 2008 text Journal of Fish Biology 2008 72 2002 2010 Ribosomal RNA genes are interspersed throughout heterochromatin chromosome arms in Atlantic salmon. AM Pendás author 1993 text Cytogenetics and Cell Genetics 1993 63 128 130 Cytogenetic analysis using quantitative, high sensitivity, fluorescence hybridization. D Pinkel author 1986 text Proceedings of the National Academy of Sciences USA 1986 83 2934 2938 AB Poletto author 2010 The B chromosomes of the African cichlid fish Haplochromis obliquidens harbour 18S rRNA gene copies. BMC Genetics 11: 1. 2010 B chromosomes in Astyanax scabripinnis (Pisces, Characidae). LB Salvador author 1992 text Heredity 1992 69 50 56 MJJ Silva author 2004 B chromosomes in Brazilian rodents. Cytogenetic and Genome Research106: gap 257–263. 2004 Inactive ribosomal cistrons are spread throughout the B chromosomes ofRattus rattus (Rodentia, Muridae). Implications for their origin and evolution. S Stitou author 2000 text Chromosome Research 2000 8 305 311 Nucleolus size variation during meiosis and NOR activity of a B chromosome in the grasshopper Eyprepocnemis plorans. M Teruel author 2007 text Chromosome Research 2007 15 755 765 Microdissection and chromosome painting of X and B chromosomes in Locusta migratoria. M Teruel author 2009 text Chromosome Research 2009 17 11 18 Chromosome mapping and molecular characterization of ribosomal RNA genes in Lebias fasciata (Teleostei, Cyprinodontidae). C Tigano author 2004 text Genetica 2004 121 95 100 Cytogenetic diversity in the Astyanax scabripinnis species complex (Pisces, Characidae). I. Allopatric distribution in a small stream. BJ Turner author 1995 text Cytologia 1995 60 1 11 Satellite DNA in Neotropical fishes: Methods, applications and perspectives. MR Vicari author 2010 text Journal of Fish Biology 2010 76 1094 1116 New insights on the origin of B chromosomes in Astyanax scabripinnis obtained by chromosome painting and FISH. MR Vicari author 2011 text Genetica 2011 139 1073 1081 Sex-ratio distortion associated with the presence of B chromosome in Astyanax scabripinnis (Teleostei, Characidae). VE Vicente author 1996 text Cytogenetics and Cell Genetics 1996 74 70 75 10.3897/compcytogen.v6i1.2151 https://compcytogen.pensoft.net/article/1746/ https://compcytogen.pensoft.net/article/1746/download/pdf/ https://compcytogen.pensoft.net/article/1746/download/xml/ Ninety individuals of the characid fish Astyanax fasciatus (Cuvier, 1819) were collected at Água da Madalena stream (Botucatu, São Paulo, Brazil) and analyzed for diploid chromosome number 2n and karyotype composition as well as for the chromosomal location of the 5S and 18S ribosomal DNA (rDNA). Whereas no chromosome differences were associated with sex, three different karyomorphs with diploid chromosome numbers 2n=46, 2n=48 and 2n=50 were found. No intermediate 2n numbers were discovered. The 2n=50 karyomorph showed some differences in 18S rDNA location compared to the two other karyomorphs. Finally, all specimens with the 2n=46 karyomorph showed the presence of a partly heterochromatic macro supernumerary chromosome, which was absent in all individuals with the two other karyomorphs. All these results suggest that indviduals of the three different karyomorphs are not likely to hybridize in the examined populations. Our findings strongly suggest the presence of three separate species (sensu biological species concept) easily diagnosed on the basis of differences in the diploid chromosome numbers and other chromosomal markers. text/html en_US Pensoft Publishers fish cytogenetics chromosome banding rDNA sympatric differentiation B chromosome Three sympatric karyomorphs in the fish Astyanax fasciatus (Teleostei, Characidae) do not seem to hybridize in natural populations Research Article

10.3897/compcytogen.v6i1.1873 2012-02-14 compcytogen

UNIPAR, Marialva, Brazil author Martinez, Emanuel EMBRAPA, Palmas, Brazil author Alves, Anderson Universidade Estadual Paulista (UNESP), Botucatu, Brazil author Silveira, Sara M Universidade Estadual Paulista (UNESP), Botucatu, Brazil author Foresti, Fausto https://orcid.org/0000-0002-0862-0445 Universidade Estadual Paulista, Botucatu, Brazil author Oliveira, Claudio 2012-02-14 2012-02-14 2012 Comparative Cytogenetics 1993-078X 1993-0771 6 1 41 51 2012 Problems of karyotypic variability in the family Characidae (Pisces, Characiformes) with the description of somatic karyotypes for six species of tetras. VA Arefjev author 1990 text Caryologia 1990 43 305 319 Relationships of the Characidiinae and phylogeny of characiform fishes (Teleostei: Ostariophisy). PA Buckup author LR Malabarba author 1998 text Edipucrs, Porto Alegre 1998 123 144 PA Buckup author 2007 Catálogo das Espécies de Peixes de Água Doce do Brasil. 2007 Nuclear content of thirty species of Neotropical fishes. ML Carvalho author 1998 text Genetics and Molecular Biology 1998 21 47 54 Cytogenetic analysis of five species of the subfamily Tetragonopterinae (Teleostei, Characiformes, Characidae). ML Carvalho author 2002a text Caryologia 2002a 55 181 188 Nuclear DNA content determination in Characiformes fish (Teleostei, Ostariophysi) from the Neotropical region. ML Carvalho author 2002b text Genetics and Molecular Biology 2002b 25 49 55 Chromosomal Differentiation among Allopatric Populations of Hyphessobrycon anisitsi (Pisces, Tetragonopterinae). L Centofante author 2003 text Cytologia 2003 68 283 288 Chromosome R-banding pattern and conservation of a marker chromosome in four species, genus Astyanax (Characidae, Tetragonopterinae). MFZ Daniel-Silva author 2001 text Caryologia 2001 54 209 215 Cytogenetic and comparative morphology of two allopatric populations of Astyanax altiparanae Garutti & Britski, 2000 (Teleostei, Characidae) from upper rio Paraná basin. MS Domingues author 2007 text Neotropical Ichthyology 2007 5 37 44 Cytogenetic studies in two populations of the Astyanax altiparanae (Pisces, Characiformes). CA Fernandes author 2004 text Hereditas 2004 141 328 332 Mapping of the 18S and 5S ribosomal RNA genes in Astyanax altiparanae Garutti & Britski, 2000 (Teleostei, Characidae) from the upper Paraná river basin, Brazil. CA Fernandes author 2006 text Genetics and Molecular Biology 2006 29 464 468 Polymorphic nature of nucleolus organizer regions in fishes. F Foresti author 1981 text Cytogenetic Cell Genetics 1981 31 137 144 Trends in chromosome evolution of Neotropical characiforms fishes. PM Galetti Jr author 1994 text Caryologia 1994 47 289 297 Descrição de uma nova espécie de Astyanax (Teleostei, Characidae) da bacia do Alto Paraná e considerações sobre as demais espécies do gênero na bacia. V Garutti author 2000 text Comunicações do Museu de Ciências e Tecnologia da Pucrs, Porto Alegre, Série Zoologia 2000 13 65 88 J Géry author 1977 Characoids of the World. 1977 WM Howell author 1980 Controlled silver staining of Nucleolus Organizer Regions with a protective colloidal developer: a 1-step method. Experientia 36: 1014 1015. 1980 Citogenética de Astyanax fasciatus e Astyanax bimaculatus (Characidae, Tetragonopterinae). SN Jin author 1975 text Ciência e Cultura 1975 27 1122 1124 Nomenclature for centromeric position on chromosomes. A Levan author 1964 text Hereditas 1964 52 201 220 Genera Incertae Sedis in Characidae. FCT Lima author RE Reis author 2003 text 1st edition. Edipucrs, Porto Alegre 2003 106 169 Weighted parsimony phylogeny of the family Characidae (Teleostei: Characiformes). JM Mirande author 2009 text Cladistics 2009 25 574 613 Cytogenetic considerations on the genus Astyanax (Pisces, Characidae). I. Kariotypic Variability. S Morelli author 1983 text Caryologia 1983 36 235 244 JS Nelson author 2006 Fishes of the World, Fourth Edition. 2006 MF Neto author 2009 Comparative cytogenetics among populations of Astyanax altiparanae (Characiformes, Characidae, Incertae sedis). Genetics and Molecular Biology 32. 2009 Cytotypes and Multiple NORs in an Astyanax altiparanae population (Pisces, Tetragonopterinae). RB Pacheco author 2001 text Chromosome Science 2001 5 109 114 RE Reis author 2003 Check List of the Freshwater Fishes of South and Central America. 2003 Evolucionary diversity reverse (R) fluorescent chromosomes bands in vertebrates. M Schmid author 1989 text Chromosoma 1989 97 101 114 Reverse fluorescent chromosome banding with Chromomycin and DAPI. D Schweiser author 1976 text Chromosoma 1976 92 143 148 JJ Sheel author 1973 Fish Chromosome and Their Evolution. 1973 A simple technique for demonstrating centromeric heterochromatin. AT Sumner author 1972 text Experimental Cell Research 1972 75 304 306 Higher level phylogenetic concepts within characiforms (Ostariophysi), a historical review. RP Vari author LR Malabarba author 1998 text Edipucrs, Porto Alegre 1998 111 122 Comparative cytogenetics among species of the Astyanax scabripinnis complex. Evolutionary and biogeographical inferences. MR Vicari author 2008 text Genetics and Molecular Biology 2008 31 173 179 10.3897/compcytogen.v6i1.1873 https://compcytogen.pensoft.net/article/1745/ https://compcytogen.pensoft.net/article/1745/download/pdf/ https://compcytogen.pensoft.net/article/1745/download/xml/ Cytogenetic analyses were accomplished in two populations of Astyanax altiparanae Garutti & Britzki, 2000 and one population of Hyphessobrycon eques Steindachner, 1882, considered incertae sedis in Characidae family. Two populations of A. altiparanae (Mogi-Guaçu and Tietê rivers) presented 2n=50, with the same karyotype formula: 6M+12SM+20ST+12A (FN=88). H. eques from Capivara river presented 2n=52 and karyotype formula 14M+16SM+4ST+18A (FN=86). In each karyotype, the nucleolus organizer regions were detected at the end of the short arm of a single medium-sized subtelocentric chromosome. The Chromomycin A3 (CMA3) marking is coincident for the NORs in chromosomes of the two species and present additionally in two different chromosomes of A. altiparanae thus showing interpopulation differences in this species. In H. eques, weak heterochromatic blocks in the position of centromeres and telomeres of most chromosomes and negative C-banding for the NOR bearing chromosome were visualized. The obtained results contribute both to the understanding of karyotype evolution of these species and to the clarifying their phylogenetic relationships. text/html en_US Pensoft Publishers Ag-NOR Chromomycin A3 chromosomes evolution Neotropical fish Cytogenetic analysis in the incertae sedis species Astyanax altiparanae Garutti and Britzki, 2000 and Hyphessobrycon eques Steindachner, 1882 (Characiformes, Characidae) from the upper Paraná river basin Research Article

10.3897/compcytogen.v6i1.2523 2012-02-14 compcytogen

University of Florida, Gainesville , United States of America author Majure, Lucas https://orcid.org/0000-0002-0369-1906 University of Florida, Gainesville, United States of America author Judd, Walter University of Florida, United States of America author Soltis, Pamela S. https://orcid.org/0000-0001-9310-8659 University of Florida, Gainesville, United States of America author Soltis, Doug 2012-02-14 2012-02-14 2012 Comparative Cytogenetics 1993-078X 1993-0771 6 1 53 77 2012 Polyploidy and genome evolution in plants. KL Adams author 2005 text Current Opinion in Plant Biology 2005 8 135 141 Population dynamics of Pinus taeda L. based on nuclear microsatellites. MA Al-Rabab’ah author 2002 text Forest Ecology and Management 2002 163 263 271 Examining genetic structure in a bogus yucca moth: sequential approach to phylogeography. 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SD Webb author RL Myers author 1990 text University Press of Florida, Gainesville 1990 70 100 Chromosome numbers in Chihuahuan Desert Cactaceae. Trans-Pecos, Texas. JF Weedin author 1978 text American Journal of Botany 1978 65 531 537 Chromosome numbers in Chihuahuan Desert Cactaceae. II. Trans-Pecos, Texas. JF Weedin author 1989 text The Southwestern Naturalist 1989 34 160 164 Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. TJ White author MA Innis author 1990 text Academic Press, San Diego 1990 315 322 Ulmus americana (Ulmaceae) is a polyploid complex. AT Whittemore author 2011 text American Journal of Botany 2011 98 754 760 The dynamic nature of apomixis in the angiosperms. J Whitton author 2008 text International Journal of Plant Sciences 2008 169 169 182 10.3897/compcytogen.v6i1.2523 https://compcytogen.pensoft.net/article/1744/ https://compcytogen.pensoft.net/article/1744/download/pdf/ https://compcytogen.pensoft.net/article/1744/download/xml/ Ploidy has been well studied and used extensively in the genus Opuntia to determine species boundaries, detect evidence of hybridization, and infer evolutionary patterns. We carried out chromosome counts for all members of the Humifusa clade to ascertain whether geographic patterns are associated with differences in ploidy. We then related chromosomal data to observed morphological variability, polyploid formation, and consequently the evolutionary history of the clade. We counted chromosomes of 277 individuals from throughout the ranges of taxa included within the Humifusa clade, with emphasis placed on the widely distributed species, O. humifusa (Raf.) Raf., 1820 s.l. and O. macrorhiza Engelm., 1850 s.l. We also compiled previous counts made for species in the clade along with our new counts to plot geographic distributions of the polyploid and diploid taxa. A phylogeny using nuclear ribosomal ITS sequence data was reconstructed to determine whether ploidal variation is consistent with cladogenesis. We discovered that diploids of the Humifusa clade are restricted to the southeastern United States (U.S.), eastern Texas, and southeastern New Mexico. Polyploid members of the clade, however, are much more widely distributed, occurring as far north as the upper midwestern U.S. (e.g., Michigan, Minnesota, Wisconsin). Morphological differentiation, although sometimes cryptic, is commonly observed among diploid and polyploid cytotypes, and such morphological distinctions may be useful in diagnosing possible cryptic species. Certain polyploid populations of O. humifusa s.l. and O. macrorhiza s.l., however, exhibit introgressive morphological characters, complicating species delineations. Phylogenetically, the Humifusa clade forms two subclades that are distributed, respectively, in the southeastern U.S. (including all southeastern U.S. diploids, polyploid O. abjecta Small, 1923, and polyploid O. pusilla (Haw.) Haw., 1812) and the southwestern U.S. (including all southwestern U.S. diploids and polyploids). In addition, tetraploid O. humifusa s.l., which occurs primarily in the eastern U.S., is resolved in the southwestern diploid clade instead of with the southeastern diploid clade that includes diploid O. humifusa s.l. Our results not only provide evidence for the polyphyletic nature of O. humifusa and O. macrorhiza, suggesting that each of these represents more than one species, but also demonstrate the high frequency of polyploidy in the Humifusa clade and the major role that genome duplication has played in the diversification of this lineage of Opuntia s.s. Our data also suggest that the southeastern and southwestern U.S. may represent glacial refugia for diploid members of this clade and that the clade as a whole should be considered a mature polyploid species complex. Widespread polyploids are likely derivatives of secondary contact among southeastern and southwestern diploid taxa as a result of the expansion and contraction of suitable habitat during the Pleistocene following glacial and interglacial events. text/html en_US Pensoft Publishers Cactaceae chromosome numbers Opuntia humifusa Opuntia macrorhiza Pleistocene refugia polyploid complex polyploidy Cytogeography of the Humifusa clade of Opuntia s.s. Mill. 1754 (Cactaceae, Opuntioideae, Opuntieae): correlations with pleistocene refugia and morphological traits in a polyploid complex Research Article

10.3897/compcytogen.v6i1.2349 2012-02-23 compcytogen

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, author Bolsheva, Nadezhda Moscow State University, Moscow, Russia author Gokhman, Vladimir https://orcid.org/0000-0001-9909-7559 Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, author Muravenko, Olga National Academy of Sciences of Ukraine, Ukraine author Gumovsky, Alex Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, author Zelenin, Alexander 2012-02-23 2012-02-23 2012 Comparative Cytogenetics 1993-078X 1993-0771 6 1 79 92 2012 Cytotaxonomic studies of Encarsia Förster (Hymenoptera: Aphelinidae). F Baldanza author 1999 text Bulletin of Entomological Research 1999 89 209 215 Visualization of polydnavirus sequences in a parasitoid wasp chromosome. E Belle author 2002 text Journal of Virology 2002 76 5793 5796 Common methylation characteristics of sex chromosomes in somatic and germ cells from mouse, lemur and human. 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JJFA Van Vugt author 2005 text Chromosoma 2005 114 410 419 DNA methylation in plants. BF Vanyushin author 2006 text Current topics in microbiology and immunology 2006 301 2 67 122 Disposition of ribosomal DNAs in the chromosomes of perennial oats (Poaceae: Aveneae). G Winterfeld author 2007 text Botanical Journal of the Linnean Society 2007 155 193 210 10.3897/compcytogen.v6i1.2349 https://compcytogen.pensoft.net/article/1743/ https://compcytogen.pensoft.net/article/1743/download/pdf/ https://compcytogen.pensoft.net/article/1743/download/xml/ Karyotypes of Entedon cionobius Thomson, 1878 and E. cioni Thomson, 1878 (Hymenoptera: Eulophidae) were studied using DNA-binding ligands with different base specificity (propidium iodide, chromomycin A3, methyl green and DAPI; all these ligands, except for the last one, were used for the first time in parasitic wasps), C-banding, fluorescence in situ hybridization (FISH) with a 45S rDNA probe and 5-methylcytosine immunodetection. Female karyotypes of both species contain five pairs of relatively large metacentric chromosomes and a pair of smaller acrocentric chromosomes (2n = 12). As in many other Hymenoptera, males of both Entedon Dalman, 1820 species have haploid chromosome sets (n = 6). Fluorochrome staining revealed chromosome-specific banding patterns that were similar between the different fluorochromes, except for the CMA3- and PI-positive and DAPI-negative band in the pericentromeric regions of the long arms of both acrocentric chromosomes. The obtained banding patterns were virtually identical in both species and allowed for the identification of each individual chromosome. C-banding revealed a pattern similar to DAPI staining, although centromeric and telomeric regions were stained more intensively using the former technique. FISH detected a single rDNA site in the same position on the acrocentric chromosomes as the bright CMA3-positive band. Immunodetection of 5-methylcytosine that was performed for the first time in the order Hymenoptera revealed 5-methylcytosine-rich sites in the telomeric, centromeric and certain interstitial regions of most of the chromosomes. text/html en_US Pensoft Publishers Hymenoptera Eulophidae Entedon chromosomes karyotypes base-specific fluorochromes fluorescence in situ hybridization (FISH) DNA methylation Comparative cytogenetic study on two species of the genus Entedon Dalman, 1820 (Hymenoptera, Eulophidae) using DNA-binding fluorochromes and molecular and immunofluorescent markers Research Article

10.3897/compcytogen.v6i1.2017 2012-03-02 compcytogen

Voronezh State University, Voronezh, author Belousov, Mikhail Voronezh State University, Voronezh, author Mashkina, Olga Voronezh State University, Voronezh, author Popov, Vasily 2012-03-02 2012-03-02 2012 Comparative Cytogenetics 1993-078X 1993-0771 6 1 93 106 2012 Cadmium and Copper Change Root Growth and Morphology of Pinus pinea and Pinus pinaster Seedlings. I Arduini author 1994 text Phisiologia Plantarum 1994 92 4 675 680 Nitrate lead influence on cytogenetic and some biochemical parameters of scotch pine seedlings. Vestnik Tambovskogo Universiteta. MV Belousov author 2011 text Seriya: Yestestvennye i tekhnicheskie nauki 2011 16 5 1321 1324 Cellular analysis of rootgrowth Lathyrus odoratus L. under the influence of heavy metals. VP Bessonova author 1991 text Tsitologiya i Genetika 1991 25 6 18 22 Analysis of sensitivity of different criteria in cytogenetic monitoring. AK Butorina author 2000 text Russian Journal of Ecology 2000 31 3 186 189 Cytogenetic monitoring of the Usmansky and Khrenovskoy autochtonic pine stands. AK Butorina author 2007 text Biological Bulletin 2007 34 4 423 426 Toxicity of some heavy metals in vivo and in vitro in Helianthus annus. B Chakravarty author 1992 text Mutation Research 1992 283 4 287 294 Molecular mechanisms of plant metal tolerance and homeostasis. S Clemens author 2001 text Planta 2001 212 4 475 486 How plants cope with cadmium: staking all on metabolism and gene expression. G DalCorso author 2008 text Journal of Integrative Plant Biology 2008 50 10 1268 1280 Genetic Analysis of black spruce (Picea mariana) populations from dry and wet areas of metal-contaminated region in Ontario (Canada) S Dobrzeniecka author 2011 text Water, Air & Soil Pollution 2011 215 1/4 117 125 Assessment of phyto- and cytotoxic activity of compounds of heavy metals and aluminum through the root apical meristem of onion. AN Dovgalyuk author 2001 text Tsitologiya i Genetika 2001 35 1 3 9 HA Dzhuvelikyan author 1999 Ecology and man. 1999 Patterns of induction of cytogenetic effects in plants under the influence of heavy metals. TI Evseeva author 2005 text Vestnik Instituta Biologii Komi 2005 87 1 4 13 Root growth inhibition, photosynthetic pigments production and metal accumulation in Sinapsis alba as the parameters for trace metals effect determination. A Fargašová author 1998 text Bulletin of Environmental Contamination and Toxicology 1998 61 6 762 769 The molecular mechanism of interaction of Et3Pb+ with tubulin. H Faulstich author 1984 text FEBS Letters 1984 174 1 128 132 Effect of heavy metal pollution of forest soil on radial growth of Scots pine. AL Fedorkov author 2007 text Forest Pathology 2007 37 2 136 142 Yadryshkovye organizatory khromosom kak adaptivnye elementy khvoinykh vidov TG Haydarova author 1999 text Tsitologiya 1999 41 12 Using the micronucleus test in screening and monitoring of mutagens. NN Ilyinskikh author 1998 text Tsitologiya i Genetika 1998 22 1 67 71 Effect of soil cadmium on Pinus sylvestris L. seedlings. CG Kim author 2003 text Plant and Soil 2003 257 443 449 Synthesis of Phytochelatins and Homo-Phytochelatins in Pisum sativum L. S Klapheck author 1995 text Plant Physiology 1995 107 2 515 521 Molecular-genetic basis of higher plants tolerance to, and accumulation of, cadmium. OA Kulaeva author 2010 text Ekologicheskaya genetika 2010 8 3 3 15 AP Kulaichev author 2006 Metody i sredstva kompleksnogo analiza dannykh 2006 VV Kuznetsov author 2006 Fiziologiya rasteny 2006 Cytogenetic response of seed progeny of scots pine to combined anthropogenic pollution in the area of Novolipetsk metallurgical combine. OS Mashkina author 2009 text Ekologicheskaya Genetika 2009 7 3 17 29 Assessment of toxic interactions of heavy metals in a multicomponent mixture using Lepidium sativum and Spirodela polyrrhisa. D Montvydienė author 2004 text Environmental Toxicology 2004 19 4 351 358 Additional chromosome gymnosperms EN Muratova author 1999 text Tsitologiya 1999 41 12 A common highly conserved cadmium detoxification mechanism from bacteria to humans. Heavy metal tolerance conferred by the ATP-binding cassette (ABC) transporter SpHMT1 requires GSH but not metal-chelating phytochelatin peptides. S Prévéral author 2009 text Journal of Biological Chemistry 2009 284 8 4936 4943 Heavy metals in forest soils within Usmanskiy forest. Vestnik Voronezhskogo Gosudarstvennogo Universiteta. Seriya: Khimiya. Biologiya. NA Protasova author 2011 text Farmatsiya 2011 1 117 126 Effects of heavy metal pollution on genetic variation and cytological disturbances in the Pinus sylvestris L. population. W Prus-Głowacki author 2006 text Journal of Applied Genetics 2006 47 2 99 108 Colcemid and the mitotic cycle. CL Rieder author 1992 text Journal of Cell Science 1992 102 3 387 392 Overexpression of Arabidopsis thaliana tryptophan synthase beta 1 (AtTSB1) in Arabidopsis and tomato confers tolerance to cadmium stress. Hsiao PY Sanjaya author 2008 text Plant, Cell & Environment 2008 31 8 1074 1085 Genotoxic ability of cadmium, chromium and nickel salts studied by kinetochore in the cytokinesis-blocked micronucleus assay. AI Seoane author 2001 text Mutation Research 2001 490 2 99 106 Physiological Aspects of Cadmium and Lead Toxic Effects on Higher Plants. IV Seregin author 2001 text Russian Journal of Plant Physiology 2001 48 4 523 544 Genotoxic effects of heavy metals: comparative investigation with plant bioassays. H Steinkellner author 1998 text Environmental and Molecular Mutagenesis 1998 31 2 183 191 Interaction of metal salts with cytoskeletal motor protein systems R Thier author 2003 text Toxicology letters 2003 140/141 75 81 AF Titov author 2007 Resistance of plants to heavy metals 2007 Genetic analysis of Pinus banksiana and P. resinosa populations from stressed sites in Ontario. KK Vandeligt author 2011 text Chemistry & Ecology 2011 27 4 369 380 Resumption of Mitotic Activity in Allium cepa Root Tips during Treatment with Lead Salts. M Wierzbicka author 1994 text Environmental and Experimental Botany 1994 34 2 173 180 The effects of the lead (PbCl2) on mitotic cell division of Anatolian Black Pine (Pinus nigra ssp. pallasiana). E Yücel author 2008 text Biological Diversity and Conservation 2008 1 2 124 129 Expression of BjMT2, a metallothionein 2 from Brassica juncea, increases copper and cadmium tolerance in Escherichia coli and Arabidopsis thaliana, but inhibits root elongation in Arabidopsis thaliana seedlings. A Zhigang author 2006 text Journal of Experimental Botany 2006 57 14 3575 3582 Cadmium-stress in nodulated white lupin: strategies to avoid toxicity. P Zornoza author 2002 text Plant Physiology and Biochemistry 2002 40 12 1003 1009 10.3897/compcytogen.v6i1.2017 https://compcytogen.pensoft.net/article/1742/ https://compcytogen.pensoft.net/article/1742/download/pdf/ https://compcytogen.pensoft.net/article/1742/download/xml/ We studied cytogenetic reactions of Scots pine seedlings to heavy metals – lead, cupric and zinc nitrates applied at concentrations 0.5 to 2000 µM. We determined the range of concentrations of heavy metals that causes mutagenic effect. Lead was found to cause the strongest genotoxicity as manifested by significant increase in the frequency of pathological mitosis, occurrence of fragmentations and agglutinations of chromosomes, various types of bridges, and a significant number of the micronuclei which were absent in the control. Possible cytogenetic mechanisms of the cytotoxic action of heavy metals are discussed. text/html en_US Pensoft Publishers Scots pine heavy metals lead nitrates zinc nitrates cupric nitrates mitosis chromosomal abnormalities micronuclei Cytogenetic response of Scots pine (Pinus sylvestris Linnaeus, 1753) (Pinaceae) to heavy metals Data Paper

10.3897/compcytogen.v6i2.2397 2012-03-16 compcytogen

Universidade Federal de Viçosa, Viçosa, Brazil author Cardoso, Danon Clemes https://orcid.org/0000-0002-2811-2536 Universidade Federal de Ouro Preto , Ouro Preto - Minas Gerais, Brazil author Cristiano, Maykon P https://orcid.org/0000-0001-7850-9155 Universidade Federal de Viçosa, Viçosa, Brazil author Barros, Luísa Universidade Federal de Viçosa, Viçosa, Brazil author Lopes, Denilce Universidade Federal de Viçosa, Viçosa, Brazil author Pompolo, Silvia 2012-03-16 2012-03-16 2012 Comparative Cytogenetics 1993-078X 1993-0771 6 107 114 2012 B Bolton author 1994 Indentification guide to the ant genera of the world. 1994 Estudos citogenéticos em formigas neotropicais do gênero Gnamptogenys Roger (Hymenoptera, Formicidae, Ectatomminae). DS Borges author 2004 text Revista Brasileira de Entomologia 2004 48 481 484 Cytogenetic data of Partamona peckolti (Hymenoptera, Apidae, Meliponini) by C banding and fluorochrome staining with DA/CMA3 and DA/DAPI. 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CDSF Mariano author 2008 text Insect Conservation and Diversity 2008 1 161 168 Contribution of cytogenetics to the debate on the paraphyly of Pachycondyla spp. (Hymenoptera, Formicidae, Ponerinae). CDSF Mariano author 2012 text Psyche 2012 2012 1 9 DNA characterization and karyotypic evolution in the bee genus Melipona (Hymenoptera, Meliponini). MP Rocha author 2002 text Hereditas 2002 136 19 27 Citogenética da tribo Meliponini (Hymenoptera, Apidae). MP Rocha author GAR Melo author 2003 text UNESC, Criciúma, Brazil 2003 311 320 Simultaneous fluorescent staining of R bands in a specific heterochromatin regions (DA/DAPI – bands) in human chromosomes. D Schweizer author 1980 text Cytogenetics and Cell Genetics 1980 27 190 193 A simple technique for demonstrating centromeric heterochromatin. AT Sumner author 1972 text Experimental Cell Research 1972 75 304 306 AT Sumner author 2003 Chromosomes: Organization and Function. 2003 Evolution of the Neotropical ant genus Linepithema. AL Wild author 2009 text Systematic Entomology 2009 34 49 62 10.3897/compcytogen.v6i2.2397 https://compcytogen.pensoft.net/article/1757/ https://compcytogen.pensoft.net/article/1757/download/pdf/ https://compcytogen.pensoft.net/article/1757/download/xml/ In this paper we present, for the first time, a detailed karyotype characterization of a species of the genus Azteca (Dolichoderinae, Formicidae). Cerebral ganglia from Azteca trigona Emery, 1893 were excised and submitted to colchicine hypotonic solution and chromosomal preparations were analyzed through conventional staining with Giemsa, C-banding, silver nitrate staining (AgNO3) and sequential base-specific fluorochromes. The analysis shows that A. trigona has a diploid number of 28 chromosomes. The karyotype consists of five metacentric pairs, seven acrocentric pairs and two pseudo-acrocentric pairs, which represents a karyotype formula 2K= 10M + 14A + 4AM and a diploid number of the arms 2AN = 38. The analysis of heterochromatin distribution revealed a positive block on distal region of the short arm of fourth metacentric pair, which was coincident with Ag-NOR band and CMA3 fluorochrome staining, meaning that rDNA sequences are interspaced by GC-rich base pairs sequences. The C-banding also marked short arms of other chromosomes, indicating centric fissions followed by heterochromatin growth. The karyotype analysis of A. trigona allowed the identification of cytogenetic markers that will be helpful in a difficult taxonomic group as Azteca and discussion about evolutionary aspects of the genome organization. text/html en_US Pensoft Publishers Karyotype chromosome number chromosome banding ants Azteca trigona First cytogenetic characterization of a species of the arboreal ant genus Azteca Forel, 1978 (Dolichoderinae, Formicidae) Research Article

10.3897/compcytogen.v6i2.2595 2012-03-16 compcytogen

Universidade Federal de São Carlos, São Carlos, Brazil author Traldi, Josiane Universidade Estadual de Ponta Grossa, Ponta Grossa, Brazil author Vicari, Marcelo Universidade Tecnológica Federal do Paraná, Medianeira, Brazil author Blanco, Daniel Universidade Federal de São Carlos, São Carlos, Brazil author Martinez, Juliana Universidade Estadual de Ponta Grossa. Depto. Biologia Estrutural, Molecular e Genética. Ponta Grossa, Ponta Grossa, Brazil author Artoni, Roberto Universidade Federal de São Carlos, Brazil author Moreira-Filho, Orlando 2012-03-16 2012-03-16 2012 Comparative Cytogenetics 1993-078X 1993-0771 6 115 125 2012 Karyotypic relationships among the tribes of Hypostominae (Siluriformes: Loricariidae) with description of XO sex chromosome system in a Neotropical fish species. AL Alves author 2006 text Genetica 2006 128 1 9 Molecular cytogenetic study of heterochromatin in Hisonotus leucofrenatus (Teleostei, Loricariidae, Hypoptopomatinae). 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CA Cramer author 2011 text Molecular Phylogenetics and Evolution 2011 59 43 52 A method for chromosome preparations from large specimens of fishes using ‘in vitro” short treatment with colchicine. F Foresti author 1993 text Experientia 1993 49 810 813 The use of clove oil as an anaesthetic and method for sampling intertidal rockpool fishes. SP Griffiths author 2000 text Journal of Fish Biology 2000 57 1453 1464 Chromosome polymorphism and constitutive heterochromatin in the Atlantic salmon, Salmo salar. SE Hartley author 1984 text Chromosoma 1984 89 377 380 Mapping of the 18S and 5S ribosomal RNA genes in the fish Prochilodus argenteus Agassiz, 1829 (Characiformes, Prochilodontidae). T Hatanaka author 2004 text Genetica 2004 122 239 244 Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. WM Howell author 1980 text Experientia 1980 36 1014 1015 A new species of Hypostomus Lacépède, 1803 (Siluriformes, Loricariidae) from the upper Rio Paraná basin, Southern Brazil. FC Jerep author 2007 text Neotropical Ichthyology 2007 5 435 442 Chromosomal location and distribution of As51 satellite DNA in five species of the genus Astyanax (Teleostei, Characidae, Incertaesedis). DLZ Kantek author 2009 text Journal of Fish Biology 2009 75 408 421 Gene mapping of 5S rDNA sites in eight fish species from the Paraiba do Sul river basin, Brazil. KF Kavalco author 2004 text Cytogenetic and Genome Research 2004 106 107 110 Karyotypic diversity and evolution of Loricariidae (Pisces, Siluriformes). KF Kavalco author 2005 text Heredity 2005 94 180 186 Nomenclature for centromeric position on chromosomes. A Levan author 1964 text Hereditas 1964 52 201 220 First description of B chromosomes in the family Auchenipteridae, Parauchenipterus galeatus (Siluriformes) of the São Francisco River basin (MG, Brazil). RL Lui author 2009 text Mícron 2009 40 552 559 TC Machado author 2011 Chromosomal painting and ZW sex chromosomes differentiation in Characidium (Characiformes, Crenuchidae). BMC Genetics 12: 65. 2011 Accentuated polymorphism of heterochromatin and nucleolar organizer regions in Astyanax scabripinnis (Pisces, Characidae): tools for understanding karyotipic evolution. M Mantovani author 2000 text Genetica 2000 109 161 168 Chromosomal diversification in ribosomal DNA sites in Ancistrus Kner, 1854 (Loricariidae, Ancistrini) from three hydrographic basins of Mato Grosso, Brazil. S Mariotto author 2011 text Comparative Cytogenetic 2011 5 289 300 Evidence for the inheritance of silver-stained nucleolus organizer regions. VD Markovic author 1978 text Human genetics 1978 41 181 187 Chromosomal localization of 5S DNA genes in Leporinus fish (Anostomidae, Characiformes). C Martins author 1999 text Chromosome Research 1999 7 363 367 Description of karyotype in Hypostomus regani (Iheringi, 1905) (Teleostei, Loricariidae) from the Piumhi river in Brazil with comments on karyotype variation found in Hypostomus. EO Mendes-Neto author 2011 text Comparative Cytogenetics 2011 5 2 133 142 Kariotypic studies of some species of the family Loricariidae (Pisces). JL Michelle author 1977 text Cytologia 1977 42 536 549 On the diploid state of the fish order Ostariophysi. I Muramoto author 1968 text Chromosoma 1968 24 59 66 Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. D Pinkel author 1986 text Proceedings of the National Academy of Sciences of the United States of America 1986 83 2934 2938 Delturinae, a new loricariid catfish subfamily (Teleostei, Siluriformes), with revisions of Delturus and Hemipsilichthys. RE Reis author 2006 text Zoological Journal of the Linnean Society 2006 147 277 299 Cytogenetic characterization of Hypostomus nigromaculatus (Siluriformes: Loricariidae). M Rubert author 2008 text Neotropical Ichthyology 2008 6 93 100 T Schwarzacher author 2000 Practical In Situ Hybridization. Oxford, 203 pp. 2000 Constitutive heterochromatin and Ag-NOR polymorphisms in the small characid fish Astyanax scabripinnis (Jenyns, 1842). IL Souza author 2007 text Cytologia 2007 72 63 69 A simple technique for demonstrating centromeric heterochromatin. AT Sumner author 1972 text Experimental Cell Research 1972 75 304 306 Heterochromatin polymorphism associated with 18S rDNA: a differential pathway among Hoplias malabaricus fish populations. MR Vicari author 2003 text Cytogenetic and Genome Research 2003 101 24 28 Subfamily Hypostominae. C Weber author RE Reis author 2003 text Porto Alegre, Edipucrs 2003 351 372 Genomic distribution of heterochromatic sequences in equids: implications to rapid chromosomal evolution. HA Wichman author 1991 text Journal of Heredity 1991 82 369 377 A new darksaddled species of Hypostomus (Siluriformes: Loricariidae) from the upper rio Paraguay basin. CH Zawadzki author 2010 text Neotropical Ichthyololgy 2010 8 719 725 K Ziemniczak author in press Comparative cytogenetics of Loricariidae (Actinopterygii: Siluriformes): emphasis in Neoplecostominae and Hypoptopomatinae. Italian Journal of Zoology. in press 10.3897/compcytogen.v6i2.2595 https://compcytogen.pensoft.net/article/1756/ https://compcytogen.pensoft.net/article/1756/download/pdf/ https://compcytogen.pensoft.net/article/1756/download/xml/ In this study, which is the first karyotype analysis of Hypostomus iheringii, nine specimens collected in Córrego da Lapa (tributary of the Passa-Cinco River) showed a diploid number of 80 chromosomes. Silver nitrate staining and fluorescence in situ hybridization (FISH) with an 18S rDNA probe revealed the presence of multiple nucleolus organizer regions (NORs) (chromosome pairs 13, 20, and 34). FISH with a 5S rDNA probe showed that this cistron was only present in chromosome pair 2. When the karyotypes of individual animals were compared, unique heterochromatic polymorphisms were detected on chromosome pairs 1 and 5. Specifically, specimens had heterochromatic blocks (h+h+) on both chromosomes, one chromosome with heterochromatic blocks (h+h-) or chromosomes that lacked heterochromatic blocks (h-h-). Considering that heteromorphic pattern is not correlated with variation in size, the process of heterochromatinization might act on the long arms of these chromosomes. In summary, all chromosomal markers indicate that the karyotype of H. iheringii is highly differentiated and that the heterochromatinization of chromosomal segments may have contributed to its karyotypic differentiation. text/html en_US Pensoft Publishers chromosome variation fish heterochromatinization Hypostomini First karyotype description of Hypostomus iheringii (Regan, 1908): a case of heterochromatic polymorphism Research Article

10.3897/compcytogen.v6i2.2575 2012-03-26 compcytogen

Royal holloway, University of London, United Kingdom author Angus, Robert https://orcid.org/https://orcid.org/0000-0002-3860-5617 (private citizen), Egham, United Kingdom author Edwards, David Gestión del Patrimonio Bioespeleológico, Santander, Spain author Luque, Carlos Gestión del Patrimonio Bioespeleológico, Santander, Spain author Labrada, Lucia 2012-03-26 2012-03-26 2012 Comparative Cytogenetics 1993-078X 1993-0771 6 127 139 2012 C Alegre author 1983 Étude cytogénetique de cinq espèces de Troglocharinus et de Speonomus delarouzeei (Col. Catopidae). Mémoires de Biospéologie 10: 363–367. 1983 Conservative chromosome number in the Cholevidae family of cave beetles. RA Buzila author 2000 text Mémoires de Biospéologie 2000 27 21 23 Étude cytogénétique de deux espèces de Speonomus (Coléoptères Bathysciinae). J Durand author 1980 text Mémoires de Biospéologie 1980 7 267 271 LA Dutton author 2007 A karyosystematic investigation of a group of sibling species related to Stictotarsus griseostriatus (De Geer) (Coleoptera: Dytiscidae). Comp. Cytogenet. 1: 3 – 16. 2007 J Fresneda author 2007 Phylogeny of the western Mediterranean Leptodirini, with emphasis on genital characters (Coleoptera: Leiodidae: Cholevinae). Systematic Entomology 32 (2): 332 – 358. 2007 L’édéage, initiation aux recherches sur la systématique des Coléoptères. R Jeannel author 1955 text Publications du Museum National d’Histoire Naturelle, Paris 1955 16 1 155 Phylogenetic problems, current classification and generic catalogue of world Leiodidae (including Cholevidae). AF Newton author PM Giachino author 1998 text Proceedings of XX International Congress of Entomology, Firenze, 1996. Atti Museo Regionale di Scienze Naturali, Torino 1998 41 178 Catalogue des Coléoptères Leiodidae Cholevinae et Platypsyllinae. M Perreau author 2000 text Mémoires de la Société Entomologique de France 2000 4 1 460 Leiodidae. M Perreau author I Löbl author 2004 text Volume 2. Apollo Books, Stenstrup 2004 133 203 I Ribera author 2010 Ancient origin of a Western Mediterranean radiation of subterranean beetles. BMC Evolutionary Biology 10:29. 2010 JM Salgado author 2008 Coleoptera, Cholevidae. Ramos MA et al. (Eds) In: Fauna Ibérica. Volume 31. 2008 Un nuevo género y nueva especie de Leptodirini troglobio de la Cordillera Cantábrica (Cantabria, España): Fresnedaella lucius n. gen., n. sp. (Coleoptera: Leiodidae: Cholevinae). JM Salgado author 2011 text Heteropterus Revista de Entomología 2011 11 1 1 12 JM Salgado author in press Revisión del género Cantabrogeus Salgado, 2000 con la descripción de tres nuevas especies hipogeas endémicas de la Cordillera Cantábrica (Coleoptera, Leiodidae, Cholevinae, Leptodirini). Animal Biodiversity and Conservation 35. in press SG Smith author 1950 The cyto-taxonomy of Coleoptera. Canadian Entomologist 82 (3): 58 – 68. 1950 SG Smith author 1978 Animal Cytogenetics 3: Insecta 5: Coleoptera. x + 366 pp. Gebrüder Borntraeger, Berlin – Stuttgart. 1978 A Stamatakis author 2008 A rapid bootstrap algorithm for the RAxML web servers. Systematic Biology57 (5): 758–771.  2008 10.3897/compcytogen.v6i2.2575 https://compcytogen.pensoft.net/article/1755/ https://compcytogen.pensoft.net/article/1755/download/pdf/ https://compcytogen.pensoft.net/article/1755/download/xml/ Karyotypes are shown for Leiodes calcarata (Erichson, 1845), Catops coracinus Kellner, 1846, Cantabrogeus luquei (Salgado 1993), Espanoliella luquei Salgado & Fresneda, 2005, Fresnedaella lucius Salgado, Labrada & Luque 2011, Notidocharis uhagoni (Sharp, 1872), Quaestus (Quaesticulus) pasensis Salgado, Labrada & Luque, 2010, all of which are shown to have a diploid number of 20 autosomes plus Xy (♂) or XX (♀) sex chromosomes, as well as an as yet undescribed triploid species of the genus Cantabrogeus Salgado, 2000. These results are contrasted with published information, all on Leptodirini, which lists 10 species asdiploid numbers of 22 + Xy or XX. It is shown that the higher chromosome number (n = 11 + X or y) previously reported refers exclusively to the more derived Leptodirini (“infraflagellates”) whereas the lower number (n = 10 + X or y) refers to the less derived surface-dwelling forms and the less derived Leptodirini (“supraflagellates”). text/html en_US Pensoft Publishers Chromosomes Leiodidae Leptodirini Leiodes Latreille 1796 Catops Paykull 1798 Cantabrogeus Salgado 2000 Espanoliella Georgiev 1976 Fresnedaella Salgado Labrada & Luque 2011 Notidocharis Jeannel 1956 Quaestus Schaufuss 1861 Cantabria trip A chromosomal investigation of some European Leiodidae (Coleoptera), with particular focus on Spanish subterranean Leptodirini Review Article

10.3897/compcytogen.v6i2.1769 2012-04-09 compcytogen

Universidade Estadual Paulista (UNESP), Brazil author Hashimoto, Diogo Universidade Estadual Paulista (UNESP), Brazil author Voltolin, Tatiana Universidade Estadual Paulista (UNESP), Brazil author Paes, Ana Universidade Estadual Paulista (UNESP), Botucatu, Brazil author Foresti, Fausto https://orcid.org/0000-0002-0862-0445 Universidade Estadual Paulista (UNESP), Brazil author Bortolozzi, Jehud Universidade Estadual Paulista, UNESP, Bauru, Brazil author Porto-Foresti, Fabio https://orcid.org/0000-0001-8845-3845 2012-04-09 2012-04-09 2012 Comparative Cytogenetics 1993-078X 1993-0771 6 141 151 2012 Population differences in the expression of nucleolus organizer regions in the grasshopper Eyprepocnemis plorans. M Bakkali author 2001 text Protoplasma 2001 217 185 190 Cytogenetic characterization of Metynnis maculatus (Teleostei; Characiformes): the description in Serrasalminae of a small B chromosome bearing inactive NOR-like sequences. S Baroni author 2009 text Caryologia 2009 2 95 101 New species of Moenkhausia Eigenmann, 1903 (Characiformes: Characidae) with comments on the Moenkhausia oligolepis species complex. RC Benine author 2009 text Neotropical Ichthyology  2009 7 161 168 Population dynamics of a selfish B chromosome neutralized by the standard genome in the grasshopper Eyprepocnemis plorans. JPM Camacho author 1997 text The American Naturalist 1997 149 1030 1050 B Chromosomes. JPM Camacho author TR Gregory author 2005 text Elsevier, San Diego 2005 223 286 Relationships between transcription, silver staining, and chromatin organization of nucleolar organizers in Secale cereale. AD Caperta author 2007 text Protoplasma 2007 232 55 59 B chromosomes: an update about their occurrence in freshwater Neotropical fishes (Teleostei). AR Carvalho author 2008 text Journal of Fish Biology 2008 72 1907 1932 Frequency increase and mitotic stabilization of a B chromosome in fish Prochilodus lineatus. ZI Cavallaro author 2000 text Chromosome Research 2000 8 627 634 Description of two new species of the genus Moenkhausia (Characiformes: Characidae) from the central Brazil. WJEM Costa author 1994 text Zoologischer Anzeiger 1994 232 21 29 Supranumerary chromosome system C-banding pattern characterization and multiple Nucleolus Organizing Region in Moenkhausia sanctaefilomenae (Pisces, Characidae). F Foresti author 1989 text Genetica 1989 79 107 114 A method for chromosome preparations from large specimens of fishes using in vitro short treatment with colchicine. F Foresti author 1993 text Experientia 1993 49 810 813 First report of a B chromosome in a natural population of Astyanax altiparanae (Characiformes, Characidae). DT Hashimoto author 2008 text Genetics and Molecular Biology 2008 31 275 278 Chromosomal features of nucleolar dominance in hybrids between the Neotropical fish Leporinus macrocephalus and Leporinus elongatus (Characiformes, Anostomidae). DT Hashimoto author 2009 text Genetica 2009 137 135 140 Chromosome mapping of H1 histone and 5S rRNA gene clusters in three species of Astyanax (Teleostei, Characiformes). DT Hashimoto author 2011 text Cytogenetic and Genome Research 2011 134 64 71 RNA viruses: hijacking the dynamic nucleolus. JA Hiscox author 2007 text Nature Reviews Microbiology 2007 5 119 127 Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. WM Howell author 1980 text Experientia 1980 36 1014 1015 Molecular characterization and chromosomal location of two families of satelite DNA in Prochilodus lineatus (Pisces, Prochilodontidae), a species with B chromosomes. CM Jesus author 2003 text Genetica 2003 118 25 32 Nomeclature for centromeric position on chromosomes. A Levan author 1964 text Hereditas 1964 52 201 220 Characidae – genera incertae sedis in Characidae. FCT Lima author RE Reis author 2003 text EDIPUCRS. Porto Alegre 2003 106 169  Astyanax scabripinnis (Pisces, Characidae): A species complex. O Moreira-Filho author 1991 text Brazilian Journal of Genetics 1991 14 331 357 B chromosomes in the fish Astyanax scabripinnis (Characidae, Tetragonopterinae): An overview in natural population. O Moreira-Filho author 2004 text Cytogenetic and Genome Research 2004 106 230 234 Morphological differentiation and possible origin of B chromosome in natural Brazilian population of Astyanax scabripinnis (Pisces, Characidae). DM Néo author 2000 text Genetica 2000 108 211 215 Chromosome formulae of neotropical freswater fishes. C Oliveira author 1988 text Brazilian Journal of Genetics 1988 11 577 624 C Oliveira author 1997 text Heredity 1997 79 473 476 Genetics of neotropical fish: from chromosomes to populations. C Oliveira author 2009 text Fish Physiology and Biochemistry 2009 35 81 100 The epigenetics of nucleolar dominance. CS Pikaard author 2000 text Trends in Genetics 2000 16 495 500 AB Poletto author 2010 The B chromosomes of the African cichlid fish Haplochromis obliquidens harbor 18S rRNA gene copies. BMC Genetics 11: 1. 2010 Considerations on the chromosome evolution of Tetragonopterinae. ALBS Portela author 1988 text Brazilian Journal of Genetics 1988 11 307 313 New occurrence of microchromosomes B in Moenkhausia sanctaefilomenae (Pisces, Characidae) from the Paraná River of Brazil: analysis of the synaptonemal complex. ALBS Portela-Castro author 2001 text Genetica 2001 110 277 283 Karyotype relationships among species of the subfamily Tetragonopterinae (Pisces, Characidae): Cytotaxonomic and evolution aspects. ALB Portela-Castro author 2002 text Cytologia 2002 67 329 336 rRNA gene silencing and nucleolar dominance: Insights into a chromosome-scale epigenetic on/off swich. S Preuss author 2007 text Biochimica et Biophysica Acta 2007 1769 383 392 The rDNA transcription machinery is assembled during mitosis in active NORs and absent in inactive NORs. P Roussel author 1996 text The Journal of Cell Biology 1996 133 235 246 A simple technique for demonstrating centromeric heterochromatin. AT Sumner author 1972 text Experimental Cell Research 1972 75 304 306 M Teruel author 2009 text Chromosoma 2009 119 291 301 Comparative cytogenetics among species of the Astyanax scabripinnis complex. evolutionary and biogeographical inferences. MR Vicari author 2008 text Genetics and Molecular Biology 2008 31 173 179 B-chromosome frequency stability in Prochilodus lineatus (Characiformes, Prochilodontidae). TA Voltolin author 2010 text Genetica 2010 138 281 284 Intraspecific crosses resulting in the first occurrence of eight and nine B chromosomes in Prochilodus lineatus (Characiformes, Prochilodontidae). TA Voltolin author 2011 text Genetics and Molecular Biology 2011 34 220 224 10.3897/compcytogen.v6i2.1769 https://compcytogen.pensoft.net/article/1754/ https://compcytogen.pensoft.net/article/1754/download/pdf/ https://compcytogen.pensoft.net/article/1754/download/xml/ The aim of this study was to characterize cytogenetically one population of the fish Moenkhausia sanctaefilomenae (Steindachner, 1907), with emphasis on the analysis of B chromosomes. The nucleolar activity in the B microchromosomes was characterized, and an analysis of mitotic instability of these microchromosomes was accomplished. The results showed a diploid chromosome number of 50 chromosomes. In all individuals, we observed the presence of B microchromosomes with intra- and inter-individual variability. The analysis of the nucleolus organizing regions (NORs) by silver nitrate staining demonstrated multiple NORs. We observed active sites of ribosomal DNA in the B microchromosomes, with a frequency of 20% in the analyzed cells, which shows gene activity in these chromosomal elements. The analysis of constitutive heterochromatin patterns showed that the B microchromosomes are heterochromatic or euchromatic, which demonstrates differentiation of DNA composition between these genomic elements. The calculation of the mitotic instability index implied that B chromosomes in this species might be in a final stage of instability. text/html en_US Pensoft Publishers fish cytogenetic NOR expression supernumerary chromosomes mitotic instability Cytogenetic analysis of B chromosomes in one population of the fish Moenkhausia sanctaefilomenae (Steindachner, 1907) (Teleostei, Characiformes) Research Article

10.3897/compcytogen.v6i2.1980 2012-04-09 compcytogen

Fundação Oswaldo Cruz, IOC, Rio de Janeiro, Brazil author Bezerra, Alexandra M. R. https://orcid.org/0000-0002-7972-5535 Faculdade Salesiana de Vitória, Vitória, Brazil author Pagnozzi, Juliana Universidade Federal de São Carlos, Sorocaba, Brazil author Carmignotto, Ana Paula Universidade de São Paulo, São Paulo, Brazil author Yonenaga-Yassuda, Yatiyo Universidade Federal de Minas Gerais, Belo Horizonte, Brazil author Rodrigues, Flávio 2012-04-09 2012-04-09 2012 Comparative Cytogenetics 1993-078X 1993-0771 6 153 161 2012 E Amante author 1975 Prejuízos causados pelo roedor Clyomys laticeps (Echimydae) em Pinus spp., em áreas reflorestadas de Sacramento, Minas Gerais. 27a ReuniãoAnual da Sociedade Brasileira para o Progresso da Ciência, São Paulo: 373–374. 1975 Taxonomia e evolução de Clyomys Thomas, 1916 (Rodentia, Echimyidae). FD Avila-Pires author 1981 text Revista Brasileira de Biologia 1981 4 529 534 Taxonomic implications of cranial morphometric variation in the genus Clyomys Thomas, 1916 (Rodentia: Echimyidae). AMR Bezerra author 2010 text Journal of Mammalogy 2010 91 1 260 272 An annotated list of caviomorph rodents collected in north-eastern Mato Grosso, Brazil. IR Bishop author 1974 text Mammalia 1974 38 489 502 Animais causando danos em plantios (Mammalia, Rodentia). CT Carvalho author 1975 text Silvicultura 1975 9 39 46 Cytogenetic variation in Ctenomys perrensi (Rodentia, Octodontidae). L Garcia author 2000 text Biological Journal of the Linnean Society 2000 71 615 624 GMT author 2009 Mercator Projection. Avaiable in http://gmt.soest.hawaii.edu/ 2009 Macrogeographic patterns of genetic differentiation in the pocket gopher Thomomys umbrinus. MS Hafner author 1987 text Systematic Zoology 1987 36 18 34 Microhabitat use among small mammals in the Brazilian Pantanal. Jr TE Lacher author 1989 text Journal of Mammalogy 1989 70 396 401 Microhabitat use among small mammals in the Brazilian Pantanal. ERRBP Leal-Mesquita author 1992 text Caryologia 1992 45 197 212 JO Moojen author 1952 A new Clyomys from Paraguay (Rodentia: Echimyidae). Journal of the Washington Academy Science 42: 102. 1952 Genetic diversity and its ecological correlates in nature: comparisons between subterranean, fossorial, and aboveground small mammals. E Nevo author E Nevo author 1990 text Progress in Clinical and Biological Research, Vol. 355. Wiley-Liss, New York 1990 347 366 An overview of evolution, systematics, population biology, cytogenetics, molecular biology and speciation in Ctenomys. OA Reig author E Nevo author 1990 text Progress in Clinical and Biological Research, Vol. 355. Wiley-Liss, New York 1990 71 96 A rapid banding technique for human chromosomes. M Seabright author 1971 text Lancet 1971 2 971 972 G- and C- band patterns and nucleolus organizer regions in somatic chromosomes of Clyomys laticeps laticeps (Rodentia, Echimyidae). MJ Souza author 1984 text Experientia 1984 40 96 97 Geomys breviceps. JM Sulentich author 1991 text Mammalian Species 1991 383 1 4 A simple technique for demonstrating centromeric heterochromatin. AT Sumner author 1972 text Experimental Cell Research 1972 75 304 306 M Svartman author 1989 Levantamento cariotípico de roedores da região do Distrito Federal. 1989 Notes on some South American Mammals, with descriptions of new species. O Thomas author 1909 text Annals and Magazine of Natural History 1909 4 230 242 Some notes on Echimyinae. O Thomas author 1916 text Annals and Magazine of Natural History 1916 18 194 303 Dynamics of a rodent assemblage in a Cerrado of Southeast Brazil. MV Vieira author 1997 text Revista Brasileira de Biologia 1997 57 99 107 Infraorder Hystricognathi Brandt, 1855. CA Woods author DE Wilson author 2005 text 3rd edition, Vol. 2. John Hopkins University Press, Baltimore, Maryland 2005 1538 1600 Karyotypes and chromosome polymorphism in Brazilian rodents. Y Yonenaga author 1975 text Caryologia 1975 28 269 286 10.3897/compcytogen.v6i2.1980 https://compcytogen.pensoft.net/article/1753/ https://compcytogen.pensoft.net/article/1753/download/pdf/ https://compcytogen.pensoft.net/article/1753/download/xml/ Clyomys Thomas, 1916 is a semifossorial rodent genus of spiny rats represented by only one species, C. laticeps, which inhabits the tropical savannas and grasslands of central Brazil and eastern Paraguay. Here we describe a new karyotype of C. laticeps found in populations of Emas National Park, Goiás state, Brazil. The four analyzed specimens had a diploid number (2n) of 32 and a fundamental autosome number (FN) of 54. Cytogenetic data include conventional staining, CBG and GTG-banding. The karyotype presents 12 meta/submetacentric pairs (1 to 12) and 3 pairs of acrocentrics (13 to 15) with gradual decrease in size. The X chromosome is a medium submetacentric and the Y is a medium acrocentric. The semifossorial habits together with habitat specificity could have contributed to the karyological variations found on this genus. text/html en_US Pensoft Publishers Clyomys Cerrado cytogenetic Echimyidae taxonomy semifossorial habit A new karyotype for the spiny rat Clyomys laticeps (Thomas, 1909) (Rodentia, Echimyidae) from Central Brazil Research Article

10.3897/compcytogen.v6i2.1739 2012-04-27 compcytogen

Universidade Estadual Paulista, Botucatu, Brazil author Valente, Guilherme Universidade Federal do Mato Grosso, Brazil author Vitorino, Carla Universidade Estadual Paulista, Brazil author Cabral-de-Mello, Diogo Universidade Estadual Paulista, Botucatu, Brazil author Oliveira, Claudio Universidade Federal do Mato Grosso, Pontal do Araguaia MT, Brazil author Souza, Issakar Universidade Estadual Paulista, Brazil author Martins, Cesar Universidade Federal do Mato Grosso, Pontal do Araguaia, Brazil author Venere, Paulo 2012-04-27 2012-04-27 2012 Comparative Cytogenetics 1993-078X 1993-0771 6 163 181 2012 Tetraploidy and the evolution of Salmonid fishes. FW Allendorf author BJ Turner author 1984 text Plenum, New York 1984 1 53 Sex chromosome evolution in fish: the formation of the neo-Y chromosome in Eigenmannia (Gymnotiformes). LF Almeida-Toledo author 2000 text Chromosoma 2000 109 3 197 200 Complex sex chromosome system in Eigenmannia sp. (Pisces, Gymnotiformes). LF Almeida-Toledo author 1984 text Genetica 1984 64 3 165 169 BrdU replication patterns demonstrating chromosome homeologies in two fish species, genus Eigenmannia. LF Almeida-Toledo author 1988 text Cytogenetic and Cell Genetic 1988 48 2 117 120 Epifluorescence and light microscopy evidencing structural and functional polymorphism of ribosomal DNA in fish (Teleostei: Astyanax fasciatus). RF Artoni author 2008 text Micron 2008 39 8 1156 1159 A “hot spot” of recombination coincides with interstitial telomeric sequence in the Armenian hamster. T Ashley author 1993 text Cytogenetic and Cell Genetic 1993 62 2–3 169 171 The X1X2Y sex chromosome system in the fish Hoplias malabaricus. I. G-, C- and chromosome replication banding. LAC Bertollo author 1997 text Chromosome Research 1997 5 7 493 499 Chromosome evolution in the erythrinid fish, Erythrinus erythrinus (Teleostei: Characiformes). LAC Bertollo author 2004 text Heredity 2004 93 228 233 Cytotaxonomic consideration on Hoplias lacerdae (Pisces, Erythrinidae) LAC Bertollo author 1978 text Brazilian Journal of Genetics 1978 1 2 103 120 Multiple sex chromosomes in the genus Hoplias (Pisces, Erythrinidae) LAC Bertollo author 1983 text Cytologia 1983 48 1 1 12 Cytotaxonomy and karyoevolution of the genus Crenicichla (perciformes, cichlidae). DC Benzaquem author 2008 text Genetics and Molecular Biology 2008 31 1 250 255 Gene mapping of 28S and 5S rDNA sites in the spined loach Cobitis taenia (Pisces, Cobitidae) from a diploid population and a diploid-tetraploid population. A Boron author 2006 text Genetica 2006 128 1–3 71 79 Karyological evidence for interspecific hybridization between Cichla monoculus and C. temensis (perciformes, cichlidae) in the amazon. MNA Brinn author 2004 text Hereditas 2004 141 3 252 257 Something from nothing: the evolution and utility of satellite repeats. AK Csink author 1998 text Trends in Genetics 1998 14 5 200 204 Chromosomal changes and adaptation of cichlid fishes during evolution. E Feldberg author AL Val author 2003 text Science Publishers INC, New Delhi, New York 2003 285 308 Age of cichlids: new dates for ancient lake fish radiations. MJ Genner author 2007 text Molecular Biology and Evolution 2007 24 5 1269 1282 Heterochromatin revisited. SIS Grewal author 2007 text Nature Reviews Genetics 2007 8 35 46 Intriguing evidence of translocations in Discus fish (Symphysodon, Cichlidae) and report of the largest meiotic chromosomal chain observed in vertebrates. MC Gross author 2009a text Heredity 2009a 102 435 441 Comparative Cytogenetic Analysis of the Genus Symphysodon (Discus Fishes, Cichlidae): Chromosomal Characteristics of Retrotransposons and Minor Ribosomal DNA. MC Gross author 2009b text Cytogenetic and Genome Research 2009b 127 1 43 53 Variability inter and intraspecific of 18S rDNA locus among Symphysodon fishes: chromosomal rearrangements. MC Gross author 2010 text Journal of Fish Biology 2010 76 5 1117 1127 Chromosome-specific alpha-satellite DNA from the centromere of chimpanzee chromosome 4. T Haaf author 1997 text Chromosoma 1997 106 4 226 232 Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. WM Howell author 1980 text Cellular and Molecular Life Sciences 1980 36 8 1014 1015 Adaptive evolution and explosive speciation: the cichlid fish model. TD Kocher author 2004 text Nature Reviews Genetics 2004 5 4 288 298 Biochemical and cytological differentiation among cichlid fishes of the Sea of Galilee IL Kornfield author 1979 text Evolution 1979 33 1 1 14 African cichlid fishes: Model system for evolutionary biology. I Kornfield author 2000 text Annual Review of Ecology and Systematics 2000 31 163 196 A phylogeny and classification of the South American Cichlidae (Teleostei: Perciformes). SO Kullander author LR Malabarba author 1998 text EDIPUCRS, Porto Alegre 1998 461 498 Family Cichlidae. SO Kullander author RE Reis author 2003 text EDIPUCRS, Porto Alegre 2003 605 654 Relative DNA content of somatic nuclei and chromosomal studies in three genera, Tilapia, Sarotherodon, and Oreochromis of the tribe Tilapiini (Pisces, Cichlidae). KC Majumdar author 1986 text Genetica 1986 68 3 175 188 Chromosome analysis of 5 species of the Cichlidae family (Pisces, Perciformes) from the Paraná River IC Martins author 1995 text Cytologia 1995 60 3 223 231 Chromosomal polymorphism and speciation in Laetacara cf. dorsigera (Teleostei, Perciformes, Cichlidae) from the river Paraná PR Brazil IC Martins-Santos author 2005 text Caryologia 2005 58 2 95 101 J Mazzuchelli author 2009 text Genetica 2009 136 3 461 469 Chromosomal variability in the wild ornamental fish Symphysodon spp. (Perciformes, Cichlidae) from Amazon. DR Mesquita author 2008 text Neotropical Ichthyology 2008 6 2 181 190 Chromosomal evidence for a putative cryptic species in the Gymnotus carapo species-complex (Gymnotiformes, Gymnotidae) SSR Milhomem author 2008 text BMC Genetics 2008 9 1 75 Cytogenetic characterization of Crenicichla (Pisces, Perciformes) of the Iguaçu River SMHK Mizoguchi author 2007 text Genetics and Molecular Research 2007 6 3 650 656 Centromeres take flight: alpha satellite and the quest for the human centromere. TD Murphy author 1998 text Cell 1998 93 3 317 320 Cytogenetic analysis on Pterophyllum scalare (perciformes, cichlidae) from Jari river, Pará state A Nascimento author 2006 text Caryologia 2006 59 3 138 143 JS Nelson author 2006 Fishes of the world. 2006 Chromosomal inversions and the reproductive isolation of species. MA Noor author 2001 text Proceedings of the National Academy of Sciences of the United States of America 2001 98 21 12084 12088 Ribosomal RNA genes are interspersed throughout a heterochromatic chromosome arm in Atlantic salmon. AM Pendás author 1993 text Cytogenetic and Cell Genetics 1993 63 2 128 130 Chromosomal studies in Crenicichla lepidota and Australoheros facetus (Cichlidae, Perciformes) from extreme Southern Brazil G Perazzo author 2010 text Reviews in Fish Biology and Fisheries 2010 21 3 509 515 Karyotype similarities among two populations of Geophagus brasiliensis (Perciformes, Cichlidae) from the Tibagi River basin/PR/Brazil LB Pires author 2008 text Caryologia 2008 61 2 135 138 Cytogenetic characterization of Geophagus brasiliensis and two species of Gymnogeophagus (Cichlidae:Geophaginae) from Guaíba Lake, RS, Brazil. LB Pires author 2010 text Folia biologica 2010 58 1–2 29 34 Chromosome differentiation patterns during cichlid fish evolution AB Poletto author 2010 text BMC Genetics 2010 11 1 50 Cichlids in aquaculture. RSV Pullin author MHA Keenleyside author 1991 text Chapman & Hall, London 1991 280 309 Chromosomal rearrangements and speciation. LH Rieseberg author 2001 text Trends in Ecology & Evolution 2001 16 7 351 358 Cytogenetic Studies and Evolutive Considerations on Fishes of the Family Cichlidae (Perciformes) from Parana River (Argentina). HA Roncati author 2007 text Cytologia 2007 72 4 379 384 Reverse fluorescent chromosome banding with chromomycin and DAPI. D Schweizer author 1976 text Chromosoma 1976 58 4 307 324 An X1X1X2X2/X1X2Y multiple sex chromosome system in a new species of the genus Gymnotus (Pisces, Gymnotiformes). EB Silva author 2005 text Environmental Biology of Fishes 2005 73 3 293 297 Phylogeny, taxonomy, and evolution of Neotropical cichlids (Teleostei: Cichlidae: Cichlinae). WL Smith author 2008 text Cladistics 2008 24 5 625 641 Molecular cytogenetics of Salminus fish (Characiformes) based on 5S and 18S rRNA genes hybridization, fluorochrome staining and C-banding. IL Souza author 2008 text Micron 2008 39 7 1036 1041 A simple technique for demonstrating centromeric heterochromatin. AT Sumner author 1972 text Experimental Cell Research 1972 75 1 304 306 Organization of repeated DNA elements in the genome of the cichlid fish Cichla kelberi and its contributions to the knowledge of fish genomes. WG Teixeira author 2009 text Cytogenetic and Genome Research 2009 125 3 224 234 A complex chromosomal polymorphism in Gobius fallax (Gobiidae, Perciformes). G Thode author 1988 text Genetica 1988 76 1 65 71 Reinterpreting pericentromeric heterochromatin. CN Topp author 2006 text Current Opinion in Plant Biology 2006 9 6 647 653 Multiple sex chromosomes in a Mexican Cyprinodontidae fish. T Uyeno author 1971 text Nature 1971 231 452 453 Second discovery of multiple sex chromosome among fishes. T Uyeno author 1972 text Experientia 1972 28 2 223 225 Cytogenetic Mapping of the Retroelements Rex1, Rex3 and Rex6 among Cichlid Fish: New Insights on the Chromosomal Distribution of Transposable Elements. GT Valente author 2011 text Cytogenetic and Genome Research 2011 133 1 34 42 Recent chromosome diversification in the evolutionary radiation of the freshwater fish family Curimatidae (Characiformes). PC Venere author 2008 text Journal of Fish Biology 2008 72 8 1976 1989 Basic and molecular cytogenetics in freshwater Cichlidae (Osteichthyes, Perciformes). Karyotypic conservadorism and divergence MR Vicari author 2006 text Caryologia 2006 59 3 260 266 Mapping 18S ribosomal genes in fish of the genus Brycon (Characidae) by fluorescence in situ hybridization (FISH). AP Wasko author 2000 text Genetics and Molecular Biology 2000 23 1 135 138 10.3897/compcytogen.v6i2.1739 https://compcytogen.pensoft.net/article/1752/ https://compcytogen.pensoft.net/article/1752/download/pdf/ https://compcytogen.pensoft.net/article/1752/download/xml/ Cichlids represent one of the most species-rich families of fishes and have attracted the attention of evolutionary biologists due to the rapid radiation occurring in some groups and the importance of some species in the world aquaculture. Cytogenetic analysis was conducted in 10 cichlid species from the Araguaia River, Amazon Basin, Brazil. The chromosome number was 2n=48 for all analyzed species except for Laetacara araguaiae Ottoni et Costa, 2009 (2n=44). Chromosomal polymorphism was detected only in Geophagus proximus (Castelnau, 1855), which exhibits an extra large submetacentric and and a dot-like chromosomes. Moreover, the C-banding revealed a general pericentromeric heterochromatic pattern and some additional blocks for some species. The heterochromatic blocks corresponding to AgNOR bearing regions were observed in all species and also corresponded to CMA3 positive blocks, which were observed in terminal regions. Besides the general conserved chromosomal and heterochromatin patterns for South American cichlids, the presence of GC-rich heterochromatin was quite different in the species Biotodoma cupido (Heckel, 1840), Geophagus proximus, Retroculus lapidifer (Castelnau, 1855), Crenicichla strigata Günther, 1862 and Heros efasciatus Heckel, 1840. The results suggest that independent events of heterochromatin modification occurred during chromosome evolution in the group, regardless of the conservation of macro-chromosomal structure. text/html en_US Pensoft Publishers chromosome evolution fish chromosomes genome Cichlidae Comparative cytogenetics of ten species of cichlid fishes (Teleostei, Cichlidae) from the Araguaia River system, Brazil, by conventional cytogenetic methods Research Article

10.3897/compcytogen.v6i2.2950 2012-04-27 compcytogen

Armenian Academy of Sciences, Yerevan, Armenia author Karagyan, Gayane https://orcid.org/0000-0001-7708-2145 Department of Entomology, Institute of Zoology Jagiellonian University, Krakow, Poland author Lachowska, Dorota https://orcid.org/0000-0001-9072-1677 Institute of Zoology of Scientific Center of Zoology and Hydroecology, National Academy of Sciences of Armenia, Yerevan, Armenia author Kalashian, Mark 2012-04-27 2012-04-27 2012 Comparative Cytogenetics 1993-078X 1993-0771 6 183 197 2012 Cytogenetic analysis of Epicauta atomaria (Meloidae) and Palembus dermestoides (Tenebrionidae) with Xyp sex determination system using standard staining, C-bands, NOR and synaptonemal complex microspreading techniques. MC Almeida author 2000 text Hereditas 2000 133 147 157 Karyotype characterization, constitutive heterochromatin and nucleolus organizer regions of Paranaita opima (Coleoptera, Chrysomelidae, Alticinae). MC Almeida author 2006 text Genetics and Molecular Biology 2006 29 3 475 481 Cytogenetic characterization of Eurysternus caribaeus (Coleoptera: Scarabaeidae): evidence of sex-autosome fusion and diploid number reduction prior to species dispersion. AP Arcanjo author 2009 text Journal of Genetics 2009 88 2 177 182 Genetic load caused by variation in the amount of rDNA in a wasp. SMR Araújo author 2002 text Chromosome Research 2002 10 607 613 Nuevo método para el estudio de cromosomas en Coleoptera a partir de hemocitos de estados larvarios. R Baragaño author 1978 text Boletín del Servicio de Plagas 1978 4 23 33 CL Bellamy author 2008a A world catalogue and bibliography of the jewel beetles (Coleoptera: Buprestoidea). Volume 1: Introduction; Fossil Taxa; Schizopodidae; Buprestidae: Julodinae - Chrysochroinae: Poecilonotini. 2008a CL Bellamy author 2008b A world catalogue and bibliography of the jewel beetles (Coleoptera: Buprestoidea). Volume 2: Chrysochroinae: Sphenopterini through Buprestinae: Stigmoderini. 2008b CL Bellamy author 2008c A world catalogue and bibliography of the jewel beetles (Coleoptera: Buprestoidea). Volume 3: Buprestinae: Pterobothrini through Agrilinae: Rhaeboscelina. 2008c CL Bellamy author 2008d A world catalogue and bibliography of the jewel beetles (Coleoptera: Buprestoidea). Volume 4: Agrilinae: Agrilina through Trachyini. 2008d CL Bellamy author 2009 A world catalogue and bibliography of the jewel beetles (Coleoptera: Buprestoidea). Volume 5: Appendices, Bibliography, Indices. 2009 A study of the constitutive heterochromatin and nucleolus organizer regions of Isocopris inhiata and Diabroctis mimas (Coleoptera: Scarabaeidae, Scarabaeinae) using C-banding, AgNO3 staining and FISH techniques. E Bione author 2005a text Genetics and Molecular Biology 2005a 28 1 111 116 Karyotype, C-and fluorescence banding pattern, NOR location and FISH study of five Scarabaeidae (Coleoptera) species. 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C Juan author 1989 text Genome 1989 32 834 839 Localization of tandemly repeated DNA sequences in beetle chromosomes by fluorescent in situ hybridization. C Juan author 1993 text Chromosome Research 1993 1 167 174 Ag-banded karyotypes in six species of jewel-beetles (Buprestidae, Coleoptera). G Karagyan author 2001 text Folia biologica (Kraków) 2001 49 1–2 42 48 Chromosome numbers and sex chromosome systems in buprestid beetles (Coleoptera, Buprestidae). Entomological Review 80(1): 38–49. GH Karagyan author 2000 text Translated from Entomologicheskoe Obozrenie 2000 79 1 96 106 New cytogenetic data on Armenian buprestids (Coleoptera, Buprestidae) with a discussion of karyotype variation within the family. G Karagyan author 2004 text Folia biologica (Kraków) 2004 52 3–4 151 158 GH Karagyan author 2007 New karyotypes of five species of jewel-beetles and presumptive ancestral state of karyotypes of the subfamilies Polycestinae, Chrysochroinae and Buprestinae (Coleoptera: Buprestidae). 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N Virkki author 1984 text Canadian Journal of Genetics and Cytology 1984 26 326 330 Staining of substances adjacent to the Xyp sex bivalent of some weevils (Coleoptera: Curculionidae). N Virkki author 1990 text Journal of Agriculture of the University of Puerto Rico 1990 74 405 418 Silver staining of the coleopteran Xyp sex bivalent. N Virkki author 1991 text Cytobios 1991 67 45 63 Ribosomal DNA location in the scarab beetle Thorectes intermedius (Costa) (Coleoptera, Geotrupidae) using banding and fluorescent in-situ hybridization. R Vitturi author 1999 text Chromosome Research 1999 7 255 260 10.3897/compcytogen.v6i2.2950 https://compcytogen.pensoft.net/article/1751/ https://compcytogen.pensoft.net/article/1751/download/pdf/ https://compcytogen.pensoft.net/article/1751/download/xml/ The male karyotypes of Acmaeodera pilosellae persica Mannerheim, 1837 with 2n=20 (18+neoXY), Sphenoptera scovitzii Faldermann, 1835 (2n=38–46), Dicerca aenea validiuscula Semenov, 1895 – 2n=20 (18+Xyp) and Sphaerobothris aghababiani Volkovitsh et Kalashian, 1998 – 2n=16 (14+Xyp) were studied using conventional staining and different chromosome banding techniques: C-banding, AgNOR-banding, as well as fluorochrome Chromomycin A3 (CMA3) and DAPI. It is shown that C-positive segments are weakly visible in all four species which indicates a small amount of constitutive heterochromatin (CH). There were no signals after DAPI staining and some positive signals were discovered using CMA3 staining demonstrating absence of AT-rich DNA and presence of GC-rich clusters of CH. Nucleolus organizing regions (NORs) were revealed using Ag-NOR technique; argentophilic material mostly coincides with positive signals obtained using CMA3 staining. text/html en_US Pensoft Publishers Coleoptera Buprestidae karyotypes Ag-banding C-banding CMA3-staining DAPI-staining Karyotype analysis of four jewel-beetle species (Coleoptera, Buprestidae) detected by standard staining, C-banding, AgNOR-banding and CMA3/DAPI staining Research Article

10.3897/compcytogen.v6i2.3107 2012-05-07 compcytogen

University of Pisa, Pisa, Italy author Bedini, Gianni https://orcid.org/0000-0002-4802-0057 University of Pisa, Pisa, Italy author Garbari, Fabio University of Pisa, Pisa, Italy author Peruzzi, Lorenzo https://orcid.org/0000-0001-9008-273X 2012-05-07 2012-05-07 2012 Comparative Cytogenetics 1993-078X 1993-0771 6 191 211 2012 Phytokaryon: a karyological database of European and Mediterranean plants. P Bareka author 2008 text Flora Mediterranea 2008 18 109 116 G Bedini author 2010 onwards Chrobase.it – chromosome numbers for the Italian flora. 2010 onwards G Bedini author 2012a Karyological knowledge of Italian vascular flora as inferred by the analysis of Chrobase.it. Plant Biosystems 145. 2012a Does chromosome number count? Mapping karyological knowledge on Italian flora in a phylogenetic framework. G Bedini author 2012b text Plant Systematics and Evolution 2012b 298 739 750 F Conti author 2005 An annotated checklist of the Italian vascular flora. 2005 Integrazioni alla checklist della flora vascolare italiana. F Conti author 2007 text Natura Vicentina 2007 10 2006 5 74 Maximum likelihood inference implies a high, not a low, ancestral haploid chromosome number in Araceae, with a critique of the bias introduced by “x”. N Cusimano author 2012 text Annals of Botany (London) 2012 109 4 681 692 A database for mapping karyological data of European and Mediterranean orchids. C Del Prete author 1999 text Acta Botanica Fennica 1999 162 155 159 Chromosome numbers and polyploidy in Polish angiosperms. P Gacek author 2011 text Acta Biologica Cracoviensia, series Botanica 2011 53 2 37 49 F Garbari author 2012 Chromosome numbers of the Italian flora. From the Caryologia foundation to present. Caryologia 65. (In press). 2012 Chromosome numbers in plant cytotaxonomy: concepts and implications. M Guerra author 2008 text Cytogenetics and Genome Research 2008 120 339 350 M Guerra author 2012 Cytotaxonomy: the end of childhood. Plant Biosystems 146(3). (In press). 2012 DA Levin author 2002 The role of chromosomal change in plant evolution. New York: 230, Oxford University Press, 230 pp. 2002 Probabilistic models of chromosome number evolution and the inference of polyploidy. I Mayrose author 2010 text Systematic Biology 2010 59 2 132 144 L Peruzzi author 2011 Chromosome number variation in two antipodean floras. AoB Plants plr020, 2011 Pathways, mechanisms, and rates of polyploid formation in flowering plants. J Ramsey author 1998 text Annual Review of Ecology and Systematics 1998 29 467 501 DG Rossiter author 2009 Technical note: Curve fitting with the R Environment for Statistical Computing. Enschede (NL): 17, International Institute for Geo-information Science & Earth Observations. 2009 TF Stuessy author 2009 Plant Taxonomy. 2009 10.3897/compcytogen.v6i2.3107 https://compcytogen.pensoft.net/article/1750/ https://compcytogen.pensoft.net/article/1750/download/pdf/ https://compcytogen.pensoft.net/article/1750/download/xml/ The Italian endemic vascular flora is composed of 1,286 specific and subspecific taxa. From the critical analysis of "Chrobase.it", 711 of them (about 55%) have been studied from a karyological point of view. These taxa belong to 52 out of 56 families and 204 out of 284 genera. These data suggest that endemic species are more studied than the flora as a whole. Mean chromosome number for Italian endemics is 2n = 30.68 ± 20.27 (median: 2n = 26, mode: 2n = 18). These values are very close to those known for the whole flora. Similar variation ranges, among endemics and species with wider distribution, are likely to reflect similar evolutionary trends. Known chromosome numbers in Italian endemics range from 2n = 8 to 2n = 182. About 9% of taxa show more than one cytotype and the frequency of Bs in the Italian endemic vascular flora is 3.3%. These values are slightly smaller compared with the whole Italian flora. Finally, for the basic chromosome numbers x = 7, 8, 9, the proportion of diploids (2n = 2x) to even polyploids (2n = 4x, 6x, 8x and 10x) can be described by the exponential function f(p) = e(5.539 – 0.637p) (R2 = 0.984). text/html en_US Pensoft Publishers B-chromosomes cytotaxonomy endemics evolution Italy polyploidy Chromosome number variation of the Italian endemic vascular flora. State-of-the-art, gaps in knowledge and evidence for an exponential relationship among even ploidy levels Research Article

10.3897/compcytogen.v6i2.1679 2012-05-23 compcytogen

Laboratório de Citogenética, ICB., Belém, Brazil author Gomes, A Universidade Federal do Para, Brazil author Nagamachi, Cleusa Laboratório de Genética and Biodiversidade, Santarém, Brazil author Rodrigues, L 4Laboratório de Genética e Biodiversidade, Brazil author Farias, Solange Laboratório de Citogenética, ICB, Belém, Brazil author Rissino, J Universidade Federal do Para, Belem, Brazil author Pieczarka, Julio 2012-05-23 2012-05-23 2012 Comparative Cytogenetics 1993-078X 1993-0771 6 213 225 2012 Karyotypes of bats of the subfamily Carolliinae (Mammalia; Phyllostomatidae) and their evolutionary implications. RJ Baker author 1971 text Experientia 1971 27 220 222 Chromosomal polymorphism in the phyllostomatid bat, Mimon crenulatum (Geoffroy). RJ Baker author 1972 text Experientia 1972 28 969 970 Evolutionary relationship of the Brachyphyllinae to Glossophaginae genera Glossophaga and Monophyllus. RJ Baker author 1979 text Journal of Mammalogy 1979 60 364 372 Karyology. 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RJ Baker author 2000 text Occasional Paper of the Museum of Texas Tech University 2000 202 1 16 Preparations of Mammalian karyotypes under field conditions. RJ Baker author 2003a text Occasional Paper of the Museum of Texas Tech University 2003a 228 1 7 Diversification among New World leaf-nosed bats: an evolutionary hypothesis and classification inferred from digenomic congruence of DNA sequence. RJ Baker author 2003b text Occasional Paper of the Museum of Texas Tech University 2003b 230 1 32 T Datzmann author 2010 Evolution of nectarivory in phyllostomid bats (Phyllostomidae Gray, 1825, Chiroptera: Mammalia). BMC Evolutionary Biology 10: 165. 2010 The comparative phylogeography of Neotropical mammals: patterns of intraspecific mitochondrial DNA variation among bats contrasted to nonvolant small mammals. AD Ditchfield author 2000 text Molecular Ecology 2000 9 1307 1318 Biogeographical karyotypic variation of Rhinophylla fischerae (Chiroptera, Phyllostomidae) suggests the occurrence of cryptic species. AJB Gomes author 2010 text Comparative Cytogenetics 2010 4 79 85 Cladistical analysis of G-banded chromosomes of nectar-feeding bats (Glossophaginae, Phyllostomidae). MW Haiduk author 1982 text Systematic Zoology 1982 31 252 265 Results of the Alcoa Foundation-Suriname expeditions. III. Chromosomal data for bats (Mammalia: Chiroptera) from Suriname. RL Honeycutt author 1980 text Annals of Carnegie Museum 1980 49 237 250 Controlled silver-staining of nucleolar organizer regions with protective colloidal developer: a 1-step method. WM Howell author 1980 text Experientia 1980 36 1014 1015 A phylogenetic supertree of the bats (Mammalia: Chiroptera). JK Jones author 2002 text Biological Reviews 2002 77 223 259 Karyotype similarity between two sympatric Schizodon fish species (Anostomidae, Characiformes) from Paraguay River basin. C Martins author 1998 text Genetics and Molecular Biology 1998 21 355 360 LJ McLellan author 2007 Subfamily Carolliinae Miller, 1924. In: A. L. Gardner (ed) Mammals of South America. Volume 1. University of Chicago Press, Chicago, 208–218. 2007 A technique to obtain fibroblast cells from skin biopsies of living bats (Chiroptera) for cytogenetic studies. R Moratelli author 2002 text Genetics and Molecular Research 2002 2 128 130 Relationships between patterns of chromosome banding and DNA synthetic sequences: a study on the chromosomes of the Seba’s fruit bat Carollia perspicilata. S Pathak author 1973 text Cytogenetics and Cell Genetics 1973 12 157 164 Chromosomal homology and evolution of phyllostomatoids bats. JC Patton author 1978 text Systematic Zoology 1978 27 449 462 Reciprocal chromosome painting between two South American bats: Carollia brevicauda and Phyllostomus hastatus (Phyllostomidae, Chiroptera). JC Pieczarka author 2005 text Chromosome Research 2005 13 349 347 Chromosome comparison between two species of Phyllsotomus (Chiroptera-Phyllostomidae) from Eastern Amazonia, with some phylogenetic insights. LRR Rodrigues author 2000 text Genetics and Molecular Biology 2000 3 593 599 Localization of rDNA genes in Phyllostomidae bats reveals silent NORs in Artibeus obscurus. N Santos author 2002 text Hereditas 2002 136 137 143 D Schweizer author 1980 Simultaneous fluorescent staining of R bands and specific heterochromatic region. l (DA-DAPI bands) in human chromosomes. Cytogenetics and Cell Genetics 27: 190–193. 1980 Mitochondrial DNA sequence, karyotypic, and Morphological variation in the Carollia castanea species complex (Chiroptera: Phyllostomidae) with description of the new species. S Solari author 2006 text Occasional Paper of the Museum of Texas Tech University 2006 254 1 16 Chromosome banding pattern homology and its phylogenetic implications in the bat genera Carollia and Choeroniscus. AD Stock author 1975 text Cytogenetics and Cell Genetics 1975 14 34 41 A simple technique for demonstrating centromeric heterochromatin. AT Sumner author 1972 text Experimental Cell Research 1972 75 304 306 LA Toledo author 1973 Estudos citogenéticos em morcego brasileiros (Mammalia Chiroptera). 1973 A survey of cytogenetic data on brazilian bats. M Varella-Garcia author 1989 text Revista Brasileira de Genética 1989 12 761 793 RS Verma author 1995 Human chromosomes: Principles and techniques. 2nd ed. 1995 ZOO-FISH analysis in a species of the order Chiroptera: Glossophaga soricina (Phyllostomidae). M Volleth author 1999 text Chromosome Research 1999 7 57 64 Phylogeny of phyllostomid bats (Mammalia: Chiroptera): data from diverse morphological systems, sex chromosomes and restriction sites. AL Wetterer author 2000 text Bulletin of the American Museum of Natural History, New York, 2000 248 1 200 Systematics of the genera Carollia and Rhinophylla based on the cytochrome-b gene. AJ Wright author 1999 text Journal of Mammalogy 1999 80 1202 1213 A comparative study of karyotypes of muntjacs by chromosome painting. F Yang author 1995 text Chromosoma 1995 103 642 652 Karyotypes of seven species of Brazilian bats. Y Yonenaga author 1969 text Caryologia 1969 22 63 78 10.3897/compcytogen.v6i2.1679 https://compcytogen.pensoft.net/article/1749/ https://compcytogen.pensoft.net/article/1749/download/pdf/ https://compcytogen.pensoft.net/article/1749/download/xml/ The family Phyllostomidae belongs to the most abundant and diverse group of bats in the Neotropics with more morphological traits variation at the family level than any other group within mammals. In this work, we present data of chromosome banding (G, C and Ag-NOR) and Fluorescence In Situ Hybridization (FISH) for representatives of Rhinophylla pumilio Peters, 1865 collected in four states of Brazil (Amazonas, Bahia, Mato Grosso and Pará). Two karyomorphs were found in this species: 2n=34, FN=64 in populations from western Pará and Mato Grosso states and 2n=34, FN=62 from Amazonas, Bahia, and northeastern Pará and Marajó Island (northern). Difference in the Fundamental Number is determined by variation in the size of the Nucleolar Organizer Region (NOR) accompanied with heterochromatin on chromosomes of pair 16 or, alternatively, a pericentric inversion. The C-banding technique detected constitutive heterochromatin in the centromeric regions of all chromosomes and on the distal part of the long arm of pair 15 of specimens from all localities. FISH with a DNA telomeric probe did not show any interstitial sequence, and an 18S rDNA probe and silver staining revealed the presence of NOR in the long arm of the pair 15, associated with heterochromatin, and in the short arm of the pair 16 for all specimens. The intra-specific analysis using chromosome banding did not show any significant difference between the samples. The comparative analyses using G-banding have shown that nearly all chromosomes of R. pumilio were conserved in the chromosome complements of Glossophaga soricina Pallas, 1766, Phyllostomus hastatus Pallas, 1767, Phyllostomus discolor Wagner, 1843 and Mimon crenulatum Geoffroy, 1801, with a single chromosomal pair unique to R. pumilio (pair 15). However, two chromosomes of M. crenulatum are polymorphic for two independent pericentric inversions. The karyotype with 2n=34, NF=62 is probably the ancestral one for the other karyotypes described for R. pumilio. text/html en_US Pensoft Publishers Biodiversity Amazon rainforest Chiroptera cytogenetics Karyotypic variation in Rhinophylla pumilio Peters, 1865 and comparative analysis with representatives of two subfamilies of Phyllostomidae (Chiroptera) Research Article

10.3897/compcytogen.v6i3.2432 2012-07-09 compcytogen

UFMG, Belo Horizonte, Brazil author Carvalho, Ana Heloisa Biodiversity Salvation, Piumhi, Brazil author Lopes, Maria Olimpia Universidade Federal de Minas Gerais, Belo Horizonte, Brazil author Svartman, Marta 2012-07-09 2012-07-09 2012 Comparative Cytogenetics 1993-078X 1993-0771 6 227 237 2012 Karyosystematics of Aepeomys and Rhipidomys (Rodentia, Cricetidae). M Aguilera author 1994 text Acta Científica Venezoelana 1994 45 247 248 Genetic studies in representatives of genus Rhipidomys (Rodentia, Sigmodontinae) from Brazil. J Andrades-Miranda author 2002 text Acta Theriologica 2002 47 125 135 Phylogeography of Rhipidomys (Rodentia, Cricetidae: Sigmodontinae) and description of two new species from southeastern Brazil. BMA Costa author 2011 text Journal of Mammalogy 2011 92 945 962 CR Bonvicino author 2008 Guia dos Roedores do Brasil, com chaves para gêneros baseadas em caracteres externos. 2008 A colchicine hypotonic citrate squash sequence for mammalian chromosome. CE Ford author 1956 text Stain Technology 1956 31 247 251 Karyotypic variation in Oryzomyini rodents (Cricetinae) with comments on chromosomal evolution in the Neotropical cricetinae complex. AL Gardner author 1976 text Occasional Papers of the Museum of Zoology LA State University 1976 9 1 48 Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. WM Howell author 1980 text Experientia 1980 36 1014 1015 Family Muridae. GG Musser author DE Wilson author 2005 text A Taxonomic and Geographic Reference. Baltimore, 3rd edition 2005 1189 1531 Composição cariotípica da fauna de roedores e marsupiais de duas áreas de Mata Atlântica do Espírito Santo, Brasil. R Paresque author 2004 text Boletim do Museu de Biologia Mello Leitão 2004 17 5 33 Mammals of Rio Juruá, Western Amazonian Brazil: Evolutionary and Ecological Diversification. JL Patton author 2000 text Bulletin American Museum Natural History 2000 244 1 306 Karyotype composition of some rodents and marsupials from Chapada Diamantina (Bahia, Brasil). LG Pereira author 2007 text Brazilian Journal of Biology 2007 67 509 518 Small mammals of the mid-Araguaia River in Central Brazil, with the description of a new species of climbing rat. RG Rocha author 2011 text Zootaxa 2011 2789 1 34 A rapid technique for human chromosomes. M Seabright author 1971 text Lancet 1971 2 971 972 Autosomal and sex chromosomal polymorphisms with multiple rearrangements and a new karyotype in the genus Rhipidomys (Sigmodontinae, Rodentia). MJJ Silva author 1999 text Hereditas 1999 131 211 220 Phylogenetics relationships and the radiation of Sigmodontinae rodents in South America: Evidence from cytochrome b. M Smith author 1999 text Journal of Mammalian Evolution 1999 6 89 128 A simple technique for demonstrating centromeric heterochromatin. AT Sumner author 1972 text Experimental Cell Research 1972 75 305 306 Pericentric inversion and X chromosome polymorphism in Rhipidomys sp. (Cricetidae, Rodentia) from Brazil. M Svartman author 1993 text Caryologia 1993 46 219 225 CJ Tribe author 1996 The Neotropical rodent genus Rhipidomys (Cricetidae, Sigmodontinae) – a taxonomic revision. 1996 VT Volobouev author 2000 Chromosome banding analysis (G-, R- and C-bands) of Rhipidomys nitela and a review of the cytogenetics of Rhipidomys (Rodentia, Sigmodontinae). Mammalia 64: 353–360. doi; 10.1515/mamm.2000.64.3.353 2000 Karyotypes of Brazilian species of Rhipidomys (Rodentia, Cricetidae). NIT Zanchin author 1992 text Journal of Mammalogy 1992 73 120 122 10.3897/compcytogen.v6i3.2432 https://compcytogen.pensoft.net/article/1766/ https://compcytogen.pensoft.net/article/1766/download/pdf/ https://compcytogen.pensoft.net/article/1766/download/xml/ In this work we present a new karyotype for Rhipidomys Tschudi, 1845 (Cricetidae, Rodentia) from Brazil. Our chromosome analyses included GTG- and CBG-banding patterns, the localization of the nucleolus organizer regions after silver staining (Ag-NORs) and fluorescence in situ hybridization (FISH) with a telomere probe. The new karyotype is composed of 44 chromosomes and has a fundamental number (number of autosomal arms) of 48. Most Rhipidomys species already karyotyped presented similar complements with 2n=44, but their fundamental numbers varied from FN=46 to 80, a variation that has been mainly attributed to pericentric inversions. The comparison of this new karyotype to those of other Rhipidomys already reported allowed us to conclude that it is a distinctive chromosome complement, which can be of great use as a tool for the very complicated taxonomic identification in this genus. text/html en_US Pensoft Publishers Rhipidomys chromosome banding FISH A new karyotype for Rhipidomys (Rodentia, Cricetidae) from Southeastern Brazil Research Article

10.3897/compcytogen.v6i3.3261 2012-07-10 compcytogen

Centre for Molecular Biotechnology, Russian State Agrarian University – Moscow Timiryazev Agricultural Academy, Moscow, author Alexandrov, Oleg Centre for Molecular Biotechnology, Russian State Agrarian University – Moscow Timiryazev Agricultural Academy, Moscow, author Divashuk, Mikhail https://orcid.org/0000-0001-6221-3659 Centre for Molecular Biotechnology, Russian State Agrarian University – Moscow Timiryazev Agricultural Academy, Moscow, author Yakovin, Nikolay Russian State Agrarian University - MTAA, Moscow, Russia author Karlov, Gennady https://orcid.org/0000-0002-9016-103X 2012-07-10 2012-07-10 2012 Comparative Cytogenetics 1993-078X 1993-0771 6 239 247 2012 Development of sex specific DNA marker for Japanese hop Humulus japonicus Siebold & Zucc. OS Alexandrov author 2011 text Russian Journal of Genetics 2011 47 8 1016 1020 Triploid Intersexes in Drosophila melanogaster. CB Bridges author 1921 text Science 1921 54 1394 252 254 Application of inter simple sequence repeat (ISSR) polymorphism for detection of sex-specific molecular markers in hop (Humulus lupulus L.). TV Danilova author 2006 text Euphytica 2006 151 1 15 21 Molecular cytogenetic mapping of Humulus lupulus sex chromosomes. MG Divashuk author 2011 text Cytogenetic and Genome Research 2011 134 3 213 219 C-Banding/DAPI and in situ hybridization reflect karyotype structure and sex chromosome differentiation in Humulus japonicus Siebold & Zucc. A Grabowska-Joachimiak author 2011 text Cytogenetic and Genome Research 2011 132 3 203 211 Genome size in Humulus lupulus L. and H. japonicus Siebold & Zucc. (Cannabaceae). A Grabowska-Joachimiak author 2006 text Acta Societatis Botanicorum Poloniae 2006 75 3 207 214 Evolution of neo-sex chromosomes in Silene diclinis. EC Howell author 2009 text Genetics 2009 182 4 1109 1115 Trinucleotide microsatellite repeat is tightly linked to male sex in hop (Humulus lupulus L.). J Jakse author 2008 text Molecular Breeding 2008 21 2 139 148 Molecular cytogenetic in hop (Humulus lupulus L.) and identification of sex chromosomes by DAPI-banding. GI Karlov author 2003 text Euphytica 2003 132 2 185 190 Organization of the KpnI family of chromosomal distal-end satellite DNA in Silene latifolia. Y Kazama author 2003 text Journal of Plant Research 2003 116 4 317 326 Karyotype analysis and physical mapping using two rRNA genes in dioecious plant, Humulus japonicus Siebold & Zucc. SY Kim author 2008 text Genes Genomics 2008 30 2 243 251 Molecular cytogenetic mapping of Cucumis sativus and C.melo using highly repetitive DNA sequences DH Koo author 2010 text Chromosome Research 2010 18 3 325 336 S Kubis author 1998 Repetitive DNA elements as a major component of plant genomes. Annals of Botany 82(Supplement A): 45–55. 1998 M Laínz author 1963 Aportaciones al conocimiento de la flora cántabro-astur, VII (1). Boletín del Instituto Estudios Asturianos. Suplemento de Ciencias 7: 45. 1963 C Linnaeus author 1753 Species plantarum: exhibentes plantas rite cognitas ad genera relatas. Tomus II. Holmiae. Salvii, 1028 pp. 1753 Sex chromosomes in flowering plants. R Ming author 2007 text American Journal of Botany 2007 94 2 141 150 Sex chromosomes in land plants. R Ming author 2011 text Annual Review of Plant Biology 2011 62 485 514 Molecular phylogeny of wild Hops, Humulus lupulus. A Murakami author 2006 text Heredity 2006 97 1 66 74 T Ono author 1955 Chromosomes of common hop and its relatives Bulletin of Brewing Science 2: 3–65. 1955 Dosage sex-chromosome systems in plants. JS Parker author 1991 text Plant Science 1991 80 1-2 79 92 Identification of sex in hop (Humulus lupulus) using molecular markers. A Polley author 1997 text Genome 1997 40 3 357 361 Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. SO Rogers author 1985 text Plant Molecular Biology Reports 1985 5 2 69 76 Cytogenetic and molecular analysis of the multiple sex chromosome system of Rumex acetosa. C Ruiz Rejón author 1994 text Heredity 1994 72 2 209 215 Male and female genetic linkage map of hops, Humulus lupulus. S Seefelder author 2000 text Plant Breeding 2000 119 3 249 255 PF Siebold author 1846 Florae Japonicae familae naturales adjectis generum et specierum exemplis selectis. Sectio altera. Plantae dicotyledoneae et monocotyledonae. Abhandelungen der mathematischphysikalischen Classe der Königlich Bayerischen Akademie der Wissenschaften 4(3): 213. 1846 Sexual development and sex chromosomes in hop. HL Shephard author 2000 text New Phytologist 2000 148 3 397 411 On the nature of sex chromosome in Humulus. O Winge author 1929 text Hereditas 1929 12 1–2 53 63 10.3897/compcytogen.v6i3.3261 https://compcytogen.pensoft.net/article/1765/ https://compcytogen.pensoft.net/article/1765/download/pdf/ https://compcytogen.pensoft.net/article/1765/download/xml/ Humulus japonicus Siebold & Zucc (Japanese hop) is a dioecious species of the family Cannabaceae. The chromosome number is 2n = 16 = 14 + XX for females and 2n = 17 = 14 + XY1Y2 for male. To date, no fluorescence in situ hybridization (FISH) markers have been established for the identification of H. japonicus sex chromosomes. In this paper, we report a method for the mitotic and meiotic sex chromosome differentiation in H. japonicus by FISH for HJSR, a high copy subtelomeric repeat. The signal is present in the subtelomeric region of one arm of the X chromosome. We demonstrate that males have two Y chromosomes that differ in FISH signal with the HJSR probe. Indeed, the HJSR probe hybridizes to a subtelomeric region on both arms of chromosome Y1 but not of chromosome Y2. The orientation and position of pseudoautosomal regions (PAR1 and PAR2) were also determined. text/html en_US Pensoft Publishers Humulus japonicus sex chromosomes sex determination in plants subtelomeric repeat fluorescence in situ hybridization Sex chromosome differentiation in Humulus japonicus Siebold & Zuccarini, 1846 (Cannabaceae) revealed by fluorescence in situ hybridization of subtelomeric repeat Research Article

10.3897/compcytogen.v6i3.2831 2012-08-09 compcytogen

Institute of Cytology and Genetics SB RAS, Novosibirsk, author Golygina, Veronika Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia author Kiknadze, Iya 2012-08-09 2012-08-09 2012 Comparative Cytogenetics 1993-078X 1993-0771 6 249 266 2012 Cytogenetic differentiation between Palearctic and Nearctic populations of Chironomus plumosus L. (Diptera, Chironomidae). MG Butler author 1999 text Genome 1999 42 5 797 815 Standartization of chromosome arms B, C and D in Chironomus (Diptera: Chironomidae). Gy Dévai author 1989 text Acta biologica Debrecina : supplementum Oecologica Hungarica 1989 2 1 79 92 VV Golygina author 1999 1999 New banding sequences in the Chironomus balatonicus karyofund (banding sequence pool). VV Golygina author 1996 text Tsitologiya 1996 38 8 869 883 Karyofund (banding species pool) of Chironomus plumosus (Diptera, Chironomidae) in Palearctic. VV Golygina author 2001 text Tsitologiya 2001 43 5 507 519 Chironomus suwai, a new species of the plumosus group (Diptera, Chironomidae) from Japan. VV Golygina author 2003 text Aquatic Insects 2003 25 3 177 189 VV Golygina author 2008 Chironomus sp. K – a new member of plumosus sibling-group from Japan. Contemporary Aquatic Entomological Study in East Asia - Proceedings of 3rd International Symposium on Aquatic Entomology in East Asia, Tianjin, China. Nankai University Press, 32–39. 2008 The revision of chromosome I (AB) mapping in Chironomus plumosus group (Diptera: CHironomidae). VV Golygina author 2008 text Comparative Cytogenetics 2008 2 1 37 55 Differentiation of the cytogenetic structure of natural populations in the plumosus group of sibling species Chironomus balatonicus, Chironomus entis, Chironomus muratensis, and Chironomus nudiventris (Chironomidae: Diptera). LI Gunderina author 1999a text Russian Journal of Genetics 1999a 35 5 506 513 Intraspecific differentiation of the cytogenetic structure in natural populations of Chironomus plumosus L., the central species in the group of sibling species (Chironomidae: Diptera). LI Gunderina author 1999b text Russian Journal of Genetics 1999b 35 2 142 150 Chromosomenevolution bei Chironomus. II. Chromosomenumbauten und phylogenetische Beziehungen der Arten. H-G Keyl author 1962 text Chromosoma 1962 13 4 464 514 The change of the pericentromeric heterochromatin mass is one of important ways of the chironomid evolution. II Kiknadze author 1991a text Tsitologiya 1991a 33 12 90 98 II Kiknadze author 1991b Karyotypes and morphology of larvae in the tribe Chironomini. Novosibirsk, 113 pp. 1991b The mapping of chromosomal arms C and D in the midge Chironomus balatonicus. II Kiknadze author 1996a text Tsitologiya 1996a 38 7 674 680 II Kiknadze author 1996b Chironomid karyofunds (banding species pools) of Yakutian cryolitozone (permafrost). Novosibirsk, 166 pp. 1996b Intercontinental karyotypic differentiation of Chironomus entis Shobanov, a Holarctic member of the C. plumosus group (Diptera, Chironomidae). II Kiknadze author 2000 text Genome 2000 43 5 857 873 Pattern of chromosomal polymorphism during population and species divergence in Chironomus (Diptera, Chironomidae). II Kiknadze author 2004 text Sibirskiy Ekologicheskiy Zhurnal 2004 11 5 635 652 A new Chironomus species of the plumosus-group (Diptera, Chironomidae) from China. II Kiknadze author 2005 text Aquatic Insects 2005 27 3 199 211 A new species of the genus Chironomus from the group plumosus - Chironomus usenicus sp. n. (Diptera, Chironomidae). NV Loginova author 1994 text Zoologicheskiy Zhurnal 1994 73 11 93 105 10.3897/compcytogen.v6i3.2831 https://compcytogen.pensoft.net/article/1764/ https://compcytogen.pensoft.net/article/1764/download/pdf/ https://compcytogen.pensoft.net/article/1764/download/xml/ A revision of the main and alternative banding sequences in chromosome II (CD) has been made for all 14 species of the Chironomus plumosus (Linnaeus, 1758) group. A new version of mapping has been suggested for 10 out of 18 banding sequences of arm C and 12 out of 22 banding sequences of arm D. Mapping of 7 banding sequences has been done for the first time according to the Keyl-Devai system. Phylogenetic relationships of banding sequences of chromosome II have been discussed. text/html en_US Pensoft Publishers Chironomus plumosus group karyotype banding sequence chromosome II mapping A revision of chromosome II (CD) mapping in Chironomus plumosus (Linnaeus, 1758) group (Diptera, Chironomidae) Research Article

10.3897/compcytogen.v6i3.3019 2012-08-20 compcytogen

Severtsov Institute of Ecology and Evolution, Moscow, Russia author Borisov, Yuri Russian Academy of Sciences, Moscow, author Orlov, Victor 2012-08-20 2012-08-20 2012 Comparative Cytogenetics 1993-078X 1993-0771 6 267 271 2012 A taxonomical reevaluation of the chromosome race Valais of the common shrew, Sorex araneus (Insectivora: Soricidae), from multiple, independent characters. H Brünner author 2002 text Acta Theriologica 2002 47 245 275 A Bukhnikashvili author 2008 Sorex satunini IUCN 2011. IUCN Red List of Threatened Species. Version 2011.1. www.iucnredlist.org 2008 Etude biométrique des mâchoires chez les Sorex du groupe araneus en Europe continentale (Mammalia, Insectivora). J Hausser author 1974 text Mammalia 1974 38 324 343 Somatic chromosomes of two species of the shrews in Caucasus. AI Kozlovsky author 1973 text Zoologicheski Zhurnal 1973 52 571 576 Chromosomal and allozyme characterization of the Caucasian shrew, Sorex satunini, from north-eastern Turkey. M Macholán author 1996 text Hereditas 1996 125 225 231 A new subspecies of the Caucasian shrew Sorex satunini (Mammalia) and phylogenetic relationships of the species inferred from mtDNA sequences and chromosomal markers. VN Orlov author 2010 text Journal of Ecology 2010 1 111 114 VA Osipova author 2006 Fossil history of shrews (Family Soricidae) in Caucasus. Ph.D. Dissertation, St. Peterburg, Russian Federation: Zoological Institute, Russian Academy of Sciences. 2006 MA Seabright author 1971 A rapid banding technique for human chromosomes. Lancet 2 (7731): 971–972. 1971 Nomenclature for the chromosomes of the common shrew (Sorex araneus). JB Searle author 2010 text Comparative Cytogenetics 2010 4 87 96 VE Sokolov author 1989 Mammals of the Caucasus. 1989 Distribution of cryptic shrew species of the genus Sorex (Mammalia) on the plain between the Don and Kuban rivers with molecular marker diagnostics. VV Stacheev author 2010 text Povolzhskiy Journal of Ecology 2010 4 396 403 The list of the chromosome races of the common shrew Sorex araneus (updated 2002). JM Wójcik author 2003 text Mammalia 2003 68 169 178 Evolution of the common shrew Sorex araneus: chromosomal and molecular aspects. JM Wójcik author J Gliwicz author 2002 text Białowieża, Polish Academy of Sciences. Acta Theriologica 47(Suppl. 1) 2002 139 167 I Zagorodniuk author 1996 Cladogenesis in the Sorex araneus group and its biogeographic interpretation. Abstracts of the ISACC Fourth International Meeting. Norr Malma and Uppsala, August 22-26, 1996. Uppsala University, Uppsala, 38. 1996 Chromosomal evolution in shrews. J Zima author JM Wójcik author 1998 text Mammal Research Institute, Polish Academy of Sciences, Białowieża 1998 175 218 10.3897/compcytogen.v6i3.3019 https://compcytogen.pensoft.net/article/1763/ https://compcytogen.pensoft.net/article/1763/download/pdf/ https://compcytogen.pensoft.net/article/1763/download/xml/ The G-banded karyotype of S. satunini was compared with the karyotype of Sorex araneus. Extensive homology was revealed. The major chromosomal rearrangements involved in the evolutionary divergence of these species have been identified as centric fusions and centromeric shifts. From the known palaeontological age of S. satunini it is obvious that the vast chromosomal polymorphism of the S. araneus group originated during the middle Pleistocene. text/html en_US Pensoft Publishers G-banding common shrew Sorex araneus Sorex satunini karyotype phylogeny A comparison of the chromosome G-banding pattern in two Sorex species, S. satunini and S. araneus (Mammalia, Insectivora) Research Article

10.3897/compcytogen.v6i3.3380 2012-08-20 compcytogen

Faculty of Science of Sfax, Sfax, Tunisia author Kharrat-Souissi, Amina Universite Paris-Sud 11, Orsay Cedex, France author Siljak-Yakovlev, Sonja University of Sarajevo, Bosnia and Herzegovina, Slovakia author Pustahija, Fatima Faculté des Sciences de Sfax, Sfax, Tunisia author Chaieb, Mohamed 2012-08-20 2012-08-20 2012 Comparative Cytogenetics 1993-078X 1993-0771 6 273 286 2012 Chromosomal location and reorganization of the 45S and 5S rDNA in the Brachyscome linearriloba complex (Asteraceae). J Adachi author 1997 text Journal Plant Research 1997 110 371 377 High-resolution physical mapping reveals that the apospory-specific genomic region (ASGR) in Cenchrus ciliaris is located on a heterochromatic and hemizygous region of a single chromosome. Y Akiyama author 2005 text Theoretical and Applied Genetics 2005 111 1042 1051 Estimation of genome size and physical mapping of ribosomal DNA in diploid and tetraploid guineagrass (Panicum maximum Jacq.). 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JS Cabral author 2006 text Genetics and Molecular Biology 2006 29 659 664 Impact of clipping on root systems of 3 grass species in Tunisia. M Chaieb author 1996 text Journal of Range Management 1996 49 336 339 Identification of homeologous chromosome pairs by in situ DNA hybridization to ribosomal RNA loci in meiotic chromosomes of cotton (Gossypium hirsutum). CF Crane author 1993 text Genome 1993 36 1015 1022 A D’Hont author 1998 Determination of basic chromosome numbers in the genus Saccharum by physical mapping of ribosomal RNA genes. Genome 41: 221–225. http://www.nrcresearchpress.com/doi/pdf/10.1139/g98-023 1998 Visualizing DNA domains and sequences by microscopy: a fifty-year history of molecular cytogenetics. H De Jong author 2003 text Genome 2003 46 943 946 Variability of the 5S and 45S rDNA sites in Passiflora L. species with distinct base chromosome number. 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K Mseddi author 2004 text African Journal of Range and Forage Science 2004 19 13 20 Physical mapping of the 18S.26S rRNA multigene family in common wheat: identification of a new locus. Y Mukai author 1991 text Chromosoma 1991 100 71 78 Does Lilium bosniacum merit species rank? A classical and molecular cytogenetic approaches. E Muratović author 2005 text Plant Systematic Evolution 2005 252 97 109 E Muratović author 2010 Speciation of European lilies from Liriotypus section based on karyotype evolution. Taxon 59: 165-175. http://www.ingentaconnect.com/content/iapt/tax/2010/00000059/00000001/art00016 2010 Molecular characterization of the genomic region linked with apomixis in Pennisetum/Cenchrus. P Ozias-Akins author 2003 text Functional and Integrative Genomics 2003 3 94 104 Origin and evolution of the South American endemic Artemisia species (Asteraceae): evidence from molecular phylogeny, ribosomal DNA and genome size data. 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Oxford University Press, New York, Oxford, 529–535. 2003 Apomictic embryo sac development in Cenchrus ciliaris (Panicoideae). NC Visser author 2000 text Bothalia 2000 30 103 110 L Watson author 1992 The Grass Genera of the World. 1992 Karyotype evolution in South American species of Hypochaeris (Asteraceae, Lactuceae). H Weiss-Schneeweiss author 2003 text Plant Systematic and Evolution 2003 241 171 184 Genome evolution in polyploids. JF Wendel author 2000 text Plant Molecular and Biology 2000 42 225 249 10.3897/compcytogen.v6i3.3380 https://compcytogen.pensoft.net/article/1762/ https://compcytogen.pensoft.net/article/1762/download/pdf/ https://compcytogen.pensoft.net/article/1762/download/xml/ The Buffelgrass (Cenchrus ciliaris L., Poaceae) is one of the most important pasturage grasses due to its high productivity and good forage qualities. This species possess a high adaptability to bioclimatic constraints of arid zones and may be used for the restoration of degraded arid ecosystems. Tunisian populations present three ploidy levels (4x, 5x and 6x) with a basic chromosome number x=9. This study reported for the first time the distribution of the ribosomal genes (rRNA) for pentaploid and hexaploid cytotypes of C. ciliaris. Molecular cytogenetic study using double fluorescence in situ hybridization has shown that the two rDNA families, 5S and 18S-5.8S-26S (18S), displayed intraspecific variation in number of loci among different ploidy levels. Each ploidy level was characterized by specific number of both 5S and 18S rDNA loci (two loci in tetraploid, five in pentaploid and six in hexaploid level). For three studied cytotypes (4x, 5x and 6x) all 5S rDNA loci were localized on the subcentromeric region of chromosomes, while 18S loci were situated on the telomeric region of short chromosome arms. Data of the FISH experiments show proportional increase of ribosomal loci number during polyploidization processes. text/html en_US Pensoft Publishers Buffelgrass Cenchrus ciliaris fluorescence in situ hybridization fluorochrome banding polyploidy rDNA organization Physical mapping of 5S and 18S-5.8S-26S RNA gene families in polyploid series of Cenchrus ciliaris Linnaeus, 1771 (Poaceae) Research Article

10.3897/compcytogen.v6i3.3543 2012-09-14 compcytogen

Faculty of Sciences - University of Lisbon, Lisbon, Portugal author Rampin, Massimiliano University of California, Museum of Vertebrate Zoology, Berkeley, United States of America author Bi, Ke University of Guelph, Department of Integrative Biology, Guelph, Canada author Bogart, James Fundacao da Faculdade de Ciencias da Universidade de Lisboa, Lisboa, Portugal author Collares Pereira, Maria Joao 2012-09-14 2012-09-14 2012 Comparative Cytogenetics 1993-078X 1993-0771 6 287 300 2012 Historical perspective of in situ hybridization for the analysis of genomic constitution of plants. FM Abbasi author 2010 text African Journal of Biotechnology 2010 9 9142 9147 Genomic in situ hybridization in plants with small genomes is feasible and elucidates the chromosomal parentage in interspecific Arabidopsis hybrids. BM Ali author 2004 text Genome 2004 47 954 960 Identification of intergenomic recombinations in unisexual salamanders of the genus Ambystoma by genomic in situ hybridization (GISH). K Bi author 2006 text Cytogenetic and Genome Research 2006 112 307 312 Intergenomic translocations in unisexual salamanders of the genus Ambystoma (Amphibia, Caudata). K Bi author 2007a text Cytogenetic and Genome Research 2007a 116 289 297 Two rare aneutriploids in the unisexual Ambystoma (Amphibia, Caudata) identified by GISH indicating two different types of meiotic errors. K Bi author 2007b text Cytogenetic and Genome Research 2007b 119 127 130 Genealogical relationships of southern Ontario polyploid unisexual salamanders (genus Ambystoma) inferred from intergenomic exchanges and major rDNA cytotypes. K Bi author 2008 text Chromosome Research 2008 16 275 289 An examination of intergenomic exchanges in A. laterale-dependent unisexual salamanders in the genus Ambystoma. 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C Sousa-Santos author 2005 text DNA Sequence 2005 16 462 467 Comparative cytogenetics of cichlid fishes through genomic in situ hybridization (GISH) with emphasis on Oreochromis niloticus. TG Valente author 2009 text Chromosome Research 2009 17 791 799 Nucleic Acids I: DNA-DNA Hybridization. SD Werman author DM Hillis author 1996 text 2nd Edition. Sinauer Associates, Sunderland, MA 1996 168 203 Kinetics of renaturation of DNA. JG Wetmur author 1968 text Journal of Molecular Biology 1968 31 349 70 Evidence for integrity of parental genomes in the diploid hybridogenetic water frog Pelophylax esculentus by Genomic in situ Hybridization. A Zalesna author 2011 text Cytogenetetic and Genome Research 2011 134 206 212 Identification of genome organization in the unusual allotetraploid form of Carassius auratus gibelio. H-P Zhu author 2007 text Aquaculture 2007 265 109 117 10.3897/compcytogen.v6i3.3543 https://compcytogen.pensoft.net/article/1761/ https://compcytogen.pensoft.net/article/1761/download/pdf/ https://compcytogen.pensoft.net/article/1761/download/xml/ Genomic In Situ Hybridization (GISH), a powerful tool to identify and to quantify genomic constituents in allopolyploids, has been widely used in plants but not in animals mainly due to technical problems in obtaining informative results. Using the allopolyploid Squalius alburnoides fish complex as a model system, we succeeded in overcoming methodological constraints when dealing with parental species with a small genome size. This hybridogenetic complex has biotypes with different genome compositions and ploidy levels, but parental chromosomes are small, morphologically very similar and therefore cannot be distinguished by conventional cytogenetic approaches. Specimens have a small genome (C-value = 1.2 pg) with a low level of highly and moderate repetitive sequences, mainly located at pericentromeric chromosome regions. Since it is well known that probe annealing depends on probe concentration and hybridization time to obtain uniform hybridization signals along the chromosome arms, we progressively increased the amount of labeled probes from 100ng up to 1µg per slide and the incubation time from overnight up to 72 h, among other minor improvements. Results showed a clear enhancement of signals with respect to previous data, allowing an accurate and reproducible assignment of the parental genomes in both diploid and triploid fish. It was thus evidenced that high probes’ concentrations and long incubation time are the key to obtain, without extra image editing, uniform and reliable hybridization signals in metaphase chromosomes of hybrid fish even involving parental species with small genome size. text/html en_US Pensoft Publishers Allopolyploids GISH C-value Squalius alburnoides complex Cyprinidae Vertebrates Identifying parental chromosomes and genomic rearrangements in animal hybrid complexes of species with small genome size using Genomic In Situ Hybridization (GISH) Research Article

10.3897/compcytogen.v6i3.3701 2012-09-14 compcytogen

Russian Academy of Sciences, Moscow, author Matveevsky, Sergey https://orcid.org/0000-0002-5808-7685 A.N. Severtsov Institute of Ecology and Evolution, Moscow, author Pavlova, Svetlana V. https://orcid.org/0000-0001-7823-9618 Chehen State University, Grosny, author Atsaeva, Maret M. Russian Academy of Sciences, Moscow, author Kolomiets, Oxana L. 2012-09-14 2012-09-14 2012 Comparative Cytogenetics 1993-078X 1993-0771 6 301 314 2012 New chromosome race and analysis of the hybrid zone between two karyomorphs of Sorex araneus (Insectivora, Soricidae). VM Aniskin author 1989 text Proceedings of the USSRAcademyofSciences 1989 309 1260 1262 Speciation by Monobrachial Centric Fusions. RJ Baker author 1986 text Proceedings of the National Academy of Sciences of USA 1986 83 8245 8248 Chromosomal evolution in Rhogeessa (Chiroptera: Vespertilionidae): Possible speciation by centric fusions. 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J Zima author JM Wójcik author 1998 text Białowieża 1998 175 218 10.3897/compcytogen.v6i3.3701 https://compcytogen.pensoft.net/article/1760/ https://compcytogen.pensoft.net/article/1760/download/pdf/ https://compcytogen.pensoft.net/article/1760/download/xml/ Immunocytochemical and electron microscopic analysis of synaptonemal complexes (SCs) was carried out for the first time in homozygotes and complex Robertsonian heterozygotes (hybrids) of the common shrew, Sorex araneus Linnaeus, 1758, from a newly discovered hybrid zone between the Moscow and the Neroosa chromosomal races. These races differ in four monobrachial homologous metacentrics, and closed SC tetravalent is expected to be formed in meiosis of a hybrid. Indeed, such a multivalent was found at meiotic prophase I in hybrids. Interactions between multivalent and both autosomes and/or the sex chromosomes were observed. For the first time we have used immunocytochemical techniques to analyse asynapsis in S. araneus and show that the multivalent pairs in an orderly fashion with complete synapsis. Despite some signs of spermatocytes arrested in the meiotic prophase I, hybrids had large number of active sperm. Thus, Moscow – Neroosa hybrid males that form a ring-of-four meiotic configuration are most likely not sterile. Our results support previous demonstrations that monobrachial homology of metacentrics of the common shrew does not lead to complete reproductive isolation between parapatric chromosomal races of the species. text/html en_US Pensoft Publishers Synaptonemal complex MSCI γH2AX centromeres Sorex araneus Synaptonemal complex analysis of interracial hybrids between the Moscow and Neroosa chromosomal races of the common shrew Sorex araneus showing regular formation of a complex meiotic configuration (ring-of-four) Research Article

10.3897/compcytogen.v6i3.2667 2012-09-26 compcytogen

EMBRAPA, Palmas, Brazil author Alves, Anderson Universidade Estadual Paulista, Rio CLaro, Brazil author de Borba, Rafael Univ Estadual Paulista, São Paulo, Brazil author Pozzobon, Allan Universidade Estadual Paulista, Botucatu, Brazil author Oliveira, Claudio Universidad de Oriente, Cumaná, Venezuela author Nirchio, Mauro Universidad de Oriente, Caicara del Orinoco, Venezuela author Granado, Ángel Universidade Estadual Paulista, Botucatu, Brazil author Foresti, Fausto 2012-09-26 2012-09-26 2012 Comparative Cytogenetics 1993-078X 1993-0771 6 315 321 2012 AL Alves author 2005 Análise da Evolução da Família Loricariidae (Telesotei, Siluriformes) Com Base em Caracteres Cromossômicos e de Seqüências de DNA. PhD Thesis, Botucatu (SP), Universidade Estadual Paulista. 2005 Karyotype variability in eight species of the subfamilies Loricariinae and Ancistrinae (Teleostei, Siluriformes, Loricariidae). AL Alves author 2003 text Caryologia 2003 56 57 63 Comparative cytogenetics analyses in eleven species of the subfamilies Neoplecostominae and Hypostominae (Siluriformes, Loricariidae). AL Alves author 2005 text Genetica 2005 124 127 136 Karyotypic relationships among the tribes of Hypostominae (Siluriformes: Loricariidae) with description of XO sex chromosome system in a Neotropical fish species. AL Alves author 2006 text Genetica 2006 128 1 9 Phylogenetic relationships of the suckermouth armoured catfishes (Loricariidae) with emphasis on the Hypostominae and the Ancistrinae. JW Armbruster author 2004 text Zoological Jounal Linnean Society 2004 141 1 80 Cytogenetic studies on Hypostominae (Pisces, Siluriformes, Loricariidae). Considerations on karyotype evolution in the genus Hypostomus. RF Artoni author 1996 text Caryologia 1996 49 1 81 90 Nature and distribution of constitutive heterochromatin in fishes, genus Hypostomus (Loricariidae). RF Artoni author 1999 text Genetica 1999 106 209 214 Identification of distinct evolutionary units in allopatric populations of Hypostomus cf. wuchereri Günther, 1864 (Siluriformes: Loricariidae): karyotypic evidence. JA Bitencourt author 2011 text Neotropical Ichthyology 2011 9 2 317 324 Chromosomal mapping of 18S-28S and 5S rRNA genes by two-colour fluoirescent in situ hybridization in six sturgeon species. F Fontana author 2003 text Genome 2003 46 473 477 Polymorphic nature of nucleolus organizer regions in fishes. F Foresti author 1981 text Cytogenetic and Cell Genetic 1981 31 137 144 A method for chromosome preparations from large specimens of fishes using in vitro short treatment with colchicine. F Foresti author 1993 text Experientia 1993 49 810 813 L Giuliano-Caetano author 1998 Polimorfismo cromossômico Robertsoniano em populações de Rineloricaria latirostris (Pisces, Loricariinae). PhD Thesis, São Carlos (SP), Departamento de Ciências Biológicas, Universidade Federal de São Carlos. 78 p. 1998 Heterochromatin characterization of four species of the family Loricariidae (Siluriformes). KF Kavalco author 2004 text Hereditas 2004 141 1 6 Karyotypic diversity and evolution of Loricariidae (Pisces, Siluriformes). KF Kavalco author 2005 text Heredity 2005 94 180 186 Description of karyotype in Hypostomus regani (Ihering, 1905) (Teleostei, Loricariidae) from the Piumhi river in Brazil with comments on karyotype variation found in Hypostomus. EO Mendes-Neto author 2011 text Comparative Cytogenetic 2011 5 2 133 142 A cytogenetic study of Diplomystes mesembrinus (Teleostei, Siluriformes, Diplomystidae) with a discussion of chromosome evolution in siluriformes. C Oliveira author 2000 text Caryologia 2000 53 1 31 37 Multiple NORs in Bryconamericus aff. exodon (Osteichthyes, Characidae, Tetragonopterinae). TR Paintner-Marques author 2002 text Hereditas 2002 137 107 112 NORs inheritance analysis in crossing including indivisuals from two stocks of rainbow trout (Oncorhynchus mykiss). F Porto-Foresti author 2002 text Hereditas 2002 136 227 230 RE Reis author 2003 Check list of the freshwater fishes of South America. Porto Alegre: Edipucrs, 729 pp. 2003 Cytogenetic characterization of Hypostomus nigromaculatus (Siluriformes: Loricariidae). M Rubert author 2008 text Neotropical Ichthyology 2008 6 1 93 100 Mapping 18S ribosomal genes in fish of the genus Brycon (Characidae) by fluorescence in situ hybridization (FISH). AP Wasko author 2000 text Genetic and Molecular Biology 2000 23 1 135 138 10.3897/compcytogen.v6i3.2667 https://compcytogen.pensoft.net/article/1759/ https://compcytogen.pensoft.net/article/1759/download/pdf/ https://compcytogen.pensoft.net/article/1759/download/xml/ The family Loricariidae with about 690 species divided into six subfamilies, is one of the world’s largest fish families. Cytogenetic studies conducted in the family showed that among 90 species analyzed the diploid number ranges from 2n=38 in Ancistrus sp. to 2n=96 in Hemipsilichthys gobio Luetken, 1874. In the present study, fluorescence in situ hybridization (FISH) was employed to determine the chromosomal localization of the 18S rDNA gene in four suckermouth armoured catfishes: Kronichthys lacerta (Nichols, 1919), Pareiorhaphis splendens (Bizerril, 1995), Liposarcus multiradiatus (Hancock, 1828) and Hypostomus prope plecostomus (Linnaeus, 1758). All species analyzed showed one chromosome pair with 18S rDNA sequences, as observed in the previous Ag-NORs analyses. The presence of size and numerical polymorphism was observed and discussed, with proposing a hypothesis of the Ag-NOR evolution in Loricariidae. text/html en_US Pensoft Publishers fish cytogenetics fluorescent in situ hybridization Loricariidae Localization of 18S ribosomal genes in suckermouth armoured catfishes Loricariidae (Teleostei, Siluriformes) with discussion on the Ag-NOR evolution Research Article

10.3897/compcytogen.v6i3.3637 2012-09-26 compcytogen

Unesp - Rio Claro, Rio Claro, Brazil author Silva, Edson https://orcid.org/0000-0002-4442-5137 Universidade Estadual Paulista, Rio CLaro, Brazil author de Borba, Rafael Universidade Federal do Mato Grosso, Brazil author Centofante, Liano UFABC, Diadema, Brazil author Miyazawa, Carlos Unesp - Rio Claro, Rio Claro, Brazil author Parise-Maltempi, Patricia 2012-09-26 2012-09-26 2012 Comparative Cytogenetics 1993-078X 1993-0771 6 323 333 2012 KT Abe author 2007 Filogenia molecular de Gasteropelecidae (Ostariophysi: Characiformes) e estudo de suas relações com outros Characiformes. Phd Dissertation, Universidade Estadual Paulista (UNESP), Botucatu, SP, Brazil. www.dominiopublico.gov.br/pesquisa/DetalheObraForm.do?select_action=&co_obra=43136 2007 Chromosome evolution in fish: sex chromosome variability in Eigenmannia virescens (Gymnotiformes. Sternopygidae). LF Almeida-Toledo author 2002 text Cytogenetic and Genome Research 2002 99 164 169 A heteromorphic ZZ/ZW sex chromosome system in fish, genus Hypostomus (Loricariidae). RF Artoni author 1998 text Cytologia 1998 63 421 425 An uncommon condition for a sex chromosome system in Characidae fish: distribution and differentiation of ZZ/ZW system in Triportheus. RF Artoni author 2001 text Chromosome Research 2001 9 449 456 Evolutionary aspects of the ZZ/ZW sex chromosome system in the Characidae fish, genus Triportheus: a monophyletic state and NOR location on the W chromosome. RF Artoni author 2002 text Heredity 2002 89 15 19 A highly differentiated ZZ/ZW sex chromosome system in a Characidae fish, Triportheus guentheri. LAC Bertollo author 1992 text Cytogenetic and Cell Genetic 1992 60 60 63 Description of a ZZ/ZW sex chromosome system in Thoracocharax cf. stellatus (Teleostei, Characiformes, Gasteropelecidae). ML Carvalho author 2002 text Genetic and Molecular Biology 2002 25 299 303 Comparative cytogenetics among sympatric species of Characidium (Pisces, Characiformes): diversity analysis with the description of ZW sex chromosome system and natural triploidy. L Centofante author 2001 text Caryologia 2001 54 253 260 A ZZ/ZW sex chromosome system in a new species of the genus Parodon (Pisces, Parodontidae). L Centofante author 2002 text Caryologia 2002 55 139 150 Chromosomal differentiation in allopatric population of Hyphessobrycon anisitsi (Pisces, Tetragonopterinae). L Centofante author 2003 text Cytologia 2003 68 283 288 CH Eigenmann author 1910 Catalogue of the freshwater fishes of tropical and south temperate America. III Zoology. Reports of the Princeton University Expeditions to Patagonia, 1896 –1899. Princeton, pp. 375–511. 1910 Polymorfic nature of nucleolus organizer regions in fishes. F Foresti author 1981 text Cytogenetic and Cell Genetic, 1981 31 137 144 J Géry author 1977 Characoids of the World. TFH publication, 672p. 1977 Cellular DNA content and evolution of teleostean fishes. R Hinegardner author 1972 text The American Naturalist 1972 106 621 644 Controlled silver staining nucleolus organizer regions with protective colloidal developer: a 1-step method. WM Howell author 1980 text Experientia 1980 36 1014 1015 Nomenclature for centromeric position on chromosomes. A Levan author 1964 text Hereditas 1964 52 201 220 Amplification of a GC-rich heterochromatin in the freshwater fish Leporinus desmotes (Characiformes, Anostomidae). VP Margarido author 2000 text Genetic and Molecular Biology 2000 23 569 573 Karyotypic differentiation through chromosome fusion and number reduction in Imparfinis hollandi (Ostariophysi, Heptapteridae). VP Margarido author 2008 text Genetic and Molecular Biology 2008 31 235 238 Extensive polymorphism and chromosomal characteristics of ribosomal DNA in the characid fish Triportheus venezuelenPageBreaksis (Characiformes, Characidae). M Nirchio author 2007 text Genetic and Molecular Biology 2007 30 25 30 Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. D Pinkel author 1986 text Proceedings of the National Academy of Sciences USA 1986 83 2934 2938 Constitutive heterochromatin, 5S and 18S rDNA genes in Apareiodon sp. (Characiformes, Parodontidae) with a ZZ/ZW sex chromosome system. R Rosa author 2006 text Genetica 2006 128 159 166 Cytogenetic characterization of Hypostomus nigromaculatus (Siluriformes: loricariidae). M Rubert author 2008 text Neotropical Ichthyology 2008 6 93 100 JJ Scheel author 1973 Fish chromosome and their evolution. Internal Report of Denmarks Akvarium, Charlottenlund, Denmark 22. 1973 Simultaneous fluorescent staining of R bands and specific heterochromatic regions (DA/DAPI bands) in human chromosomes. D Schweizer author 1980 text Cytogenetic and Cell Genetic 1980 27 190 193 Morphometrics analysis in Thoracocharax stellatus (Kner, 1858) (Characiformes, Gasteropelecidae) from different South American river basins. EL Silva author 2009 text Biota Neotropica 2009 9 71 76 Cytogenetic characterisation of the ornamental freshwater fish, Piabucus melanostomus (Iguanodectinae) from Brazilian wetlands and its relation with species of Characidae basal group. EL Silva author 2012 text Reviews in Fish Biology and Fishers 2012 22 477 484 A simple technique for demonstrating centromeric heterocromatin. AT Sumner author 1972 text Experimental Cell Research 1972 75 304 306 Comparative cytogenetics of Carnegiella marthae and Carnegiella strigata (Characiformes, Gasteropelecidae) and description of a ZZ/ZW sex chromosome system. ML Terêncio author 2008 text Genetic and Molecular Biology 2008 31 231 234 Occurrence of ZZ/ZW sex chromosomes in Thoracocharax stellatus fish (Characiformes, Gasteropelecidae) from the Araguaia River, South America. PC Venere author 2007 text Genetica 2007 133 109 112 Cytogenetics and biogeography: considerations about the natural origin of Hoplias malabricus on the Iguaçu River. MR Vicari author 2006 text Brazilian Archives of Biology and Technology 2006 49 297 303 Comparative cytogenetics among species of the Astyanax scabripinnis complex: Evolutionary and biogeographical inferences. MR Vicari author 2008 text Genetic and Molecular Biology 2008 31 173 179 The oesteology and relationships of the South American characoid fishes of the subfamily Gasteropelecinae. SH Weitzman author 1954 text Stanford Ichthyology Bulletin 1954 4 213 263 Further notes on the relationships of the South American characoid fishes of the subfamily Gasteropelecinae. SH Weitzman author 1960 text Stanford Ichthyology Bull 1960 7 217 239 Biogeography and evolutionary diversification. SH Weitzman author GT Prance author 1982 text Columbia University Press, New York 1982 403 422 SH Weitzman author 1996 Do freshwater hatchetfishes really fly? Tropical Fish Hobbyist 45: 195–206. 1996 SH Weitzman author 2003 Family Gasteropelecidae. In: Reis et al. (Eds) Checklist of the Freshwater fishes of South and Central America. Porto Alegre, Edipucrs, 101–103. 2003 10.3897/compcytogen.v6i3.3637 https://compcytogen.pensoft.net/article/1758/ https://compcytogen.pensoft.net/article/1758/download/pdf/ https://compcytogen.pensoft.net/article/1758/download/xml/ Thoracocharax stellatus (Characiformes, Gasteropelecidae) is a small Neotropical species of fish, widely distributed in several rivers of South America. Evidence for karyotype heteromorphysm in populations from different geographical regions has been reported for this species. In this way, populations of T. stellatus from the Paraguay River basin were cytogenetically characterized and the results were compared with other studies performed in the same species but from different basins. The results showed a diploid number of 2n = 54 for T. stellatus, with chromosomes arranged in 6 metacentric (m), 6 submetacentric (sm), 2 subtelocentric (st) and 40 acrocentric (a), for both sexes, with a simple Nucleolus Organiser Region (NOR) system reported by the techniques of silver nitrate impregnation and fluorescent in situ hybridisation (FISH) using 18S rDNA sequences as probe. The distribution of constitutive heterochromatin, observed by the C-band technique and Chromomycin A3 staining showed great similarity among the analyzed populations and consists mainly of discrete blocks in the pericentromeric and telomeric regions of most chromosomes. The presence of female heterogamety was also observed indicating a ZZ/ZW system with W chromosome almost totally heterochromatic. The results also show cytogenetic diversity of the group and are useful to understand the mechanisms of karyotype evolution of the family. text/html en_US Pensoft Publishers Thoracocharax stellatus Ag-NOR C-band FISH 18S rDNA Cytogenetic analysis in Thoracocharax stellatus (Kner, 1858) (Characiformes, Gasteropelecidae) from Paraguay River Basin, Mato Grosso, Brazil Research Article

10.3897/compcytogen.v6i4.3519 2012-10-05 compcytogen

Universitat de les Illes Balears, Palma de Mallorca, Spain author Petitpierre, Eduard Museo Nacional de Historia Natural, Santiago, Chile author Elgueta, Mario 2012-10-05 2012-10-05 2012 Comparative Cytogenetics 1993-078X 1993-0771 6 335 340 2012 Chromosome count in some representatives of the family Chrysomelidae (Coleoptera). L Barabás author 1978 text Biologia (Bratislava) 1978 33 8 621 625 M Daccordi author 1994 Notes for phylogenetic study of Chrysomelinae, with descriptions on new taxa and a list of all known genera (Coleoptera: Chrysomelidae, Chrysomelinae). In: Furth DG (Ed) Proceedings of the Third International Symposium on Chrysomelidae. Beijing 1992, Backhuys Publications, Leiden, 60–84. 1994 Sex chromosome rearrangements in Polyphaga beetles. AM Dutrillaux author 2009 text Sexual Development 2009 3 43 54 BD Farrell author 1998 “Inordinate fondness” explained: why are there so many beetles? Science 281: 555–559 1998 Karyotype analysis of four jewel- beetle species (Coleoptera, Buprestidae) detected by standard staining, C-banding, AgNOR-banding and CMA3/DAPI staining. G Karagyan author 2012 text Comparative Cytogenetics 2012 6 2 183 197 A new contribution to the cytogenetics and cytotaxonomy of the Chrysomelinae (Coleoptera, Chrysomelidae). E Petitpierre author 1988 text Cytobios 1988 54 153 159 A chromosome survey of three South African species of Chrysomelinae (Coleoptera: Chrysomelidae). E Petitpierre author 2004 text African Entomology 2004 12 1 123 124 A cytogenetic study of three Chilean species of Chrysomelinae (Coleoptera, Chrysomelidae). E Petitpierre author 2006 text Folia biologica (Krákow) 2006 54 3-4 87 91 Cytogenetics, cytotaxonomy and chromosomal evolution of Chrysomelinae revisited (Coleoptera, Chrysomelidae). E Petitpierre author 2011a text ZooKeys 2011a 157 67 79 Una nueva especie de coleóptero para la fauna chilena: Blaptea elguetai (Coleoptera: Chrysomelidae: Chrysomelinae). E Petitpierre author 2011b text Boletín del Museo Nacional de Historia Natural, Chile 2011b 60 63 66 A new genus of Chrysomelinae from Australia (Coleoptera: Chrysomelidae). CMA Reid author 2009 text Zootaxa 2009 2207 53 66 C-bands on chromosomes of 32 beetle species (Coleoptera: Elateridae, Cantharidae, Oedemeridae, Cerambycidae, Chrysomelidae and Curculionidae). M Rozek author 2004 text Hereditas 2004 140 1 10 SG Smith author 1978 Coleoptera. In: John B (Ed) Animal Cytogenetics vol 3: Insecta 5. 1978 N Virkki author 1984 Chromosomes in Evolution of Coleoptera. In: Sharma AK, Sharma A (Eds) Chromosomes in Evolution of Eukaryotic groups Vol 2, CRC Press, Boca Raton, Florida, 41–76. 1984 10.3897/compcytogen.v6i4.3519 https://compcytogen.pensoft.net/article/1778/ https://compcytogen.pensoft.net/article/1778/download/pdf/ https://compcytogen.pensoft.net/article/1778/download/xml/ Four species of Chilean leaf beetles in the subfamily Chrysomelinae have been cytogenetically analyzed, Blaptea elguetai Petitpierre, 2011, Henicotherus porteri Bréthes, 1929 and Jolivetia obscura (Philippi, 1864) show 2n = 28 chromosomes and a 13 + Xyp male meioformula, and Pataya nitida (Philippi, 1864) has the highest number of 2n = 38 chromosomes. The karyotype of H. porteri is made of mostly small meta/submetacentric chromosomes, and that of Jolivetia obscura displays striking procentric blocks of heterochromatin at pachytene autosomic bivalents using conventional staining. These findings are discussed in relation to previous cytogenetic data and current taxonomy of the subfamily. text/html en_US Pensoft Publishers Coleoptera Chrysomelidae Chrysomelinae karyotypes cytotaxonomy A chromosomal analysis of four species of Chilean Chrysomelinae (Coleoptera, Chrysomelidae) Research Article

10.3897/compcytogen.v6i4.4058 2012-10-05 compcytogen

Zoological Institute, St. Petersburg, St. Petersburg, author Kuznetsova, Valentina G. https://orcid.org/0000-0001-8386-5453 Bulgarian Academy of Sciences, Sofia, Bulgaria author Grozeva, Snejana https://orcid.org/0000-0003-3196-8068 Zoological Institute RAS, St.Petersburg, author Anokhin, Boris https://orcid.org/0000-0002-4110-6704 2012-10-05 2012-10-05 2012 Comparative Cytogenetics 1993-078X 1993-0771 6 341 346 2012 Repeated losses of TTAGG telomere repeats in evolution of beetles (Coleoptera). R Frydrychová author 2002 text Genetica 2002 115 179 187 Phylogenetic distribution of TTAGG telomeric repeats in insects. R Frydrychová author 2004 text Genome 2004 47 163 178 Telomere sequence localization and karyotype evolution in higher plants. J Fuchs author 1995 text Plant Systematics and Evolution 1995 196 227 241 Karyotypes, male meiosis and comparative FISH mapping of 18S ribosomal DNA and telomeric (TTAGG)n repeat in eight species of true bug (Hemiptera, Heteroptera). S Grozeva author 2011 text Comparative Cytogenetics 2011 5 4 355 374 S Grozeva author in press Sex chromosome “pre-reduction” in male meiosis of Lethocerus patruelis (Stål, 1854) (Heteroptera, Belostomatidae) with some notes on the distribution of the species. ZooKeys. in press Cytogenetics of the true bug infraorder Cimicomorpha (Hemiptera, Heteroptera): a review. VG Kuznetsova author 2011 text ZooKeys 2011 154 31 70 What Genes and Chromosomes Say about the Origin and Evolution of Insects and Other Arthropods. VA Lukhtanov author 2010 text Russian Journal of Genetics 2010 46 9 1115 1121 Systema Cryptoceratorum Phylogeneticum (Insecta, Heteroptera). M Mahner author 1993 text Zoologica 1993 48 1 302 Plant telomere biology. TD McKnight author 2004 text Plant Cell 2004 16 794 803 K Sahara author 1999 TTAGG telomeric repeats in chromosomes of some insects and other arthropods. Chromosome research 7: 449– 460. 1999 DE Scherbakov author 2002 Superorder Cimicidea Laicharting, 1781. Order Hemiptera Linne, 1758. The bugs, cicadas, plantlice, scale insects, etc. In: Rasnitsyn AP (Ed) History of Insects. DLJ Quicke, PP. 143–157. Dordrecht, The Netherlands: Kluwer Academic, 517. 2002 The telomere repeat motif of basal Metazoa. W Traut author 2007 text Chromosome Research 2007 15 3 371 382 The evolutionary origin of insect telomeric repeats, (TTAGG)n. M Vitková author 2005 text Chromosome Research 2005 13 145 156 Systematics and Evolution of Heteroptera: 25 Years of Progress. C Weirauch author 2011 text Annual Review of Entomology 2011 56 487 510 Cladistic relationships among higher groups of Heteroptera: congruence between morphological and molecular data sets. WC Wheeler author 1993 text Entomologica Scandinavica 1993 24 121 137 18S rRNA hyper-elongation and the phylogeny of Euhemiptera (Insecta: Hemiptera). Q Xie author 2008 text Molecular Phylogenetics and Evolution 2008 47 463 471 Telomeres: the beginnings and ends of eukaryotic chromosomes. VA Zakian author 2012 text Experimental Cell Research 2012 318 12 1456 1460 10.3897/compcytogen.v6i4.4058 https://compcytogen.pensoft.net/article/1777/ https://compcytogen.pensoft.net/article/1777/download/pdf/ https://compcytogen.pensoft.net/article/1777/download/xml/ Using the fluorescence in situ hybridization (FISH), the presence of (TTAGG)n telomeric sequence was detected in the chromosomes of Lethocerus patruelis (Stål, 1854) belonging to the family Belostomatidae (Heteroptera: Nepomorpha). This sequence was exclusively present at the ends of chromosomes in this species. This is the first evidence of the insect-type TTAGG telomeric repeats in Heteroptera. text/html en_US Pensoft Publishers Chromosomes FISH (TTAGG)n telomeric repeat true bugs Nepomorpha Belostomatidae Lethocerus patruelis The first finding of (TTAGG)n telomeric repeat in chromosomes of true bugs (Heteroptera, Belostomatidae) Short Communication

10.3897/compcytogen.v6i4.3183 2012-10-08 compcytogen

Institute of Biology and Soil Science, Vladivostok, Russia author Vakurin, Alexey Institute of Biology and Soil Science, author Korablev, Vladimir Kunming Institute of Zoology, China author Jiang, Xue-Long https://orcid.org/0000-0003-2052-2490 M. V. Lomonosov Moscow State University, author Grigor'eva, Tat'iana 2012-10-08 2012-10-08 2012 Comparative Cytogenetics 1993-078X 1993-0771 6 347 358 2012 A chromosome study of the pika, Ochotona princeps. CE Adams author 1971 text Mammalian Chromosome Newsletters 1971 12 3 77 78 Isolation and characterization of DNA from fixed cells and tissues. FE Arrighi author 1968 text Experimental Cell Research 1968 50 1 47 53 The chromosomes of Royle’s pika, Ochotona roylei, (Mammalia, Lagomorpha). E Capanna author 1991 text Rendiconti Lincei 1991 9 2 59 67 A colchicines hypotonic citrate squash sequence for mammalian chromosomes. CE Ford author 1956 text Stain Technology 1956 31 6 247 251 The species status of a new pika (Ochotona hoffmanni Formozov et al., 1996) and its inclusion in the list of Russian fauna. NA Formozov author 1999 text Byulleten’ Moskovskogo Obshchestva Ispytateley Prirody 1999 104 5 68 72 Taxonomic status of the Helan-Shan pika, Ochotona argentata, from the Helan-Shan Ridge (Ningxia, China). NA Formozov author 2004 text Zoologicheskiy Zhurnal 2004 83 8 995 1007 Molecular systematics of pikas of the subgenus Pika (Ochotona, Lagomorpha). NA Formozov author 2006 text Zoologicheskiy Zhurnal 2006 85 12 1465 1473 AA Gureev author 1964 Lagomorphs. 1964 I Hayata author 1969 A note on the somatic chromosomes of the Japanese pika, Ochotona hyperborea yesoensis Kishida. Journal of the Faculty of Science Hokkaido University Series 6 17(2): 393–396. 1969 Ochotonidae. RS Hoffmann author DE Wilson author 2005 text 3rd edition. Johns Hopkins University Press. Baltimore, Maryland 2005 185 193 Use of cytogenetic data in solving the problems of historical zoogeography: the intra- and intercontinental relationships of pikas (Ochotona: Lagomorpha) and common shrews (Sorex: Insectivora) EYu Ivanitskaya author AP Kryukov author 1989 text Vladivostok, The USSR Academy of Sciences 1989 74 94 Comparative analysis of banded chromosomes of pikas and intrageneric taxonomy of the genus Ochotona (Ochotonidae, Lagomorpha). EYu Ivanitskaya author 1991 text Proceedings of the Zoological Institute of the Russian Academy of Sciences 1991 243 110 126 G- and C-banding karyotypes of the Afghan pika, Ochotona rufescens. Y Kimura author 1983 text Chromosome Information Service 1983 35 13 15 Inferring divergence times within pikas (Ochotona spp.) using mtDNA and relaxed molecular dating techniques. HC Lanier author 2009 text Molecular Phylogenetics and Evolution 2009 53 1 12 Yeast stimulation of bone marrow mitosis for cytogenetics investigation. MN Lee author 1980 text Cytogenetics and Cell Genetics 1980 26 36 40 Molecular phylogenetics and taxonomy of the subgenus Pika (Ochotona, Lagomorpha). AA Lissovsky author 2007 text Journal of Mammalogy 2007 88 5 1195 1204 The oldest lagomorph (Lagomorpha, Mammalia) from the basal Eocene of Mongolia. AV Lopatin author 2008 text Proceedings of the Academy of Sciences (series: General biology) 2008 419 5 709 711 Chromosomes of three Asian mammals: Meriones meridianus (Rodentia: Gerbillinae), Spermophilopsis leptodactylus (Rodentia: Sciuridae), Ochotona rufescens (Lagomorpha: Ochotonidae). ChF Nadler author 1969 text Experientia 1969 25 7 774 775 Phylogeny of pikas (Lagomorpha, Ochotona) inferred from mitochondrial cytochrome b sequences. Y Niu author 2004 text Folia Zoologica 2004 53 2 141 155 VN Orlov author 1974 Karyosystematics of mammals. 1974 Species and distribution patterns of small mammals in the Pingheliang Nature Reserve of Qinling Mountain, Shaanxi. L Qin author 2007 text Zoological Research 2007 28 3 231 242 Somatic chromosomes of a male collared pika, Ochotona collaris (Nelson) (Lagomorpha: Ochotonidae). VR Rausch author 1973 text Mammalian Chromosome Newsletters 1973 14 109 111 Early Eocene lagomorph (Mammalia) from Western India and the early diversification of Lagomorpha. KD Rose author 2008 text Proceedings of the Royal Society B 2008 275 1639 1203 1208 VE Sokolov author 1994 Mammals of Russia and adjacent regions: Lagomorpha. 1994 Chromosome banding pattern relationships of hares, rabbits, and pikas (order Lagomorpha). AD Stock author 1976 text Cytogenetics and Cell Genetics 1976 17 78 88 A sample technique for demonstrating centromeric heterochromatin. AT Sumner author 1972 text Experimental Cell Research 1972 75 304 306 A study on karyotype of black-lipped pika (Ochotona curzoniae). P Tan author 1987 text Acta Theriologica Sinica 1987 7 3 233 235 Comparative karyology of six species of Palaearctic pikas (Ochotona, Ochotonidae, Lagomorpha). NN Vorontsov author NN Vorontsov author 1969 text Novosibirsk 1969 26 29 Comparative kariology of north Palaearctic pikas (Ochotona, Lagomorpha, Ochotonidae). NN Vorontsov author 1973 text Zoologicheskiy Zhurnal 1973 52 4 584 588 YX Wang author 2003 A complete checklist of mammal species and subspecies in China. 2003 Genome-Wide Comparative Chromosome Map between Human and the Forrest’s Pika (Ochotona forresti) Established by Cross-Species Chromosome Painting: Further Support for the Glires Hypothesis. J Ye author 2011 text Cytogenetic and Genome Research 2011 132 1–2 41 46 Molecular systematics of pikas (genus Ochotona) inferred from mitochondrial DNA sequences. N Yu author 2000 text Molecular Phylogenetics and Evolution 2000 16 1 85 95 10.3897/compcytogen.v6i4.3183 https://compcytogen.pensoft.net/article/1776/ https://compcytogen.pensoft.net/article/1776/download/pdf/ https://compcytogen.pensoft.net/article/1776/download/xml/ The karyotype of the Tsing-Ling (Huanghe) pika, Ochotona huangensis Matschie, 1908 from the forest habitats of the Qinling Mountains (Shaanxi Province, China) was described for the first time. The chromosome set contains 42 chromosomes (NFa=80). The autosomes are 15 meta-submetacentric pairs and 5 subtelocentric pairs. The X chromosome is a medium sized submetacentric; the Y chromosome is a small sized acrocentric. C-banding revealed a localization of heterochromatin in the pericentromeric regions of all autosomes. text/html en_US Pensoft Publishers Ochotona huangensis pika karyotype chromosome C-banding The chromosomes of Tsing-Ling pika, Ochotona huangensis Matschie, 1908 (Lagomorpha, Ochotonidae) Research Article cGFnZT0xJnNldD1jb21wY3l0b2dlbiZmcm9tPSZ1bnRpbD0mbWV0YWRhdGFfcHJlZml4PW1vZHM=