Latest Articles from Comparative Cytogenetics Latest 5 Articles from Comparative Cytogenetics https://compcytogen.pensoft.net/ Thu, 28 Mar 2024 22:18:22 +0200 Pensoft FeedCreator https://compcytogen.pensoft.net/i/logo.jpg Latest Articles from Comparative Cytogenetics https://compcytogen.pensoft.net/ Cytotaxonomic investigations on species of genus Narcissus (Amaryllidaceae) from Algeria https://compcytogen.pensoft.net/article/78852/ Comparative Cytogenetics 16(1): 55-76

DOI: 10.3897/compcytogen.v16.i1.78852

Authors: Naila Chahinez Boukhebache, Nabila Amirouche, Rachid Amirouche

Abstract: This paper provides new cytotaxonomic data on the genus Narcissus Linnaeus, 1753, in Algeria. Populations of seven taxa, N. tazetta Linnaeus, 1753, N. pachybolbus Durieu, 1847, N. papyraceus Ker Gawler, 1806, N. elegans (Haworth) Spach, 1846, N. serotinus sensu lato Linnaeus, 1753, including N. obsoletus (Haworth) Steudel, 1841, and N. cantabricus De Candolle, 1815, were karyologically investigated through chromosome counting and karyotype parameters. N. tazetta and N. elegans have the same number of chromosomes 2n = 2x = 20 with different karyotype formulas. Karyological and morphological characteristics, confirm the specific status of N. pachybolbus and N. papyraceus, both are diploids with 2n = 22 but differing in asymmetry indices. The morphotypes corresponding to N. serotinus sensu lato show two ploidy levels 2n = 4x = 20 and 2n = 6x = 30 characterized by a yellow corona. Some hexaploid cytotypes have more asymmetric karyotype with predominance of subtelocentric chromosomes. They are distinguished by orange corona and may correspond to N. obsoletus. Other cytotype 2n = 28 of N. serotinus was observed in the North Western biogeographic sectors. N. cantabricus was found to be diploid with 2n = 2x = 14, which is a new diploid report in the southernmost geographic range of this polyploid complex.

HTML

XML

PDF

]]>
Research Article Tue, 5 Apr 2022 14:32:06 +0300
Comparative chromosome studies in species of subtribe Orchidinae (Orchidaceae) https://compcytogen.pensoft.net/article/75990/ Comparative Cytogenetics 15(4): 507-525

DOI: 10.3897/compcytogen.v15.i4.75990

Authors: Alessio Turco, Antonella Albano, Pietro Medagli, Robert P. Wagensommer, Saverio D'Emerico

Abstract: In our study, FISH mapping using 18S-5.8S-25S rDNA and 5S rDNA sequences was performed for the first time on Ophrys tenthredinifera Willdenow, 1805, Serapias vomeracea (Burman f., 1770) Briquet, 1910 and Himantoglossum hircinum (Linnaeus, 1753) Sprengel, 1826. A detailed study was also performed on O. tenthredinifera using Giemsa-staining, silver-staining, CMA fluorescence banding and fluorescence in situ hybridisation (FISH) with rDNA probes. We analysed two subspecies, i.e. O. tenthredinifera subsp. neglecta (Parlatore, 1860) E.G. Camus, 1908 and O. tenthredinifera subsp. grandiflora (Tenore, 1819) Kreutz, 2004 by the traditional Feulgen method and constructed the karyotype. The cytotaxonomic implications for both taxa are also discussed. In Himantoglossum hircinum, FISH and silver staining highlighted differences in the number of two rDNA families (35S and 5S) with respect to Barlia robertiana (Loiseleur-Deslongchamps, 1807) Greuter, 1967. In addition, fluorescence in situ hybridisation was also applied to diploid (2n = 2x = 36) and triploid (2n = 3x = 54) Anacamptis morio (Linnaeus, 1753) R.M. Bateman, Pridgeon et M.W. Chase, 1997. As far as we are aware, this is the first case of autotriploidy observed in A. morio.

HTML

XML

PDF

]]>
Research Article Fri, 17 Dec 2021 15:05:27 +0200
Distribution, karyomorphology, and morphology of Aspidistra subrotata (Asparagaceae) at different ploidy levels in limestone areas of Asia https://compcytogen.pensoft.net/article/9803/ Comparative Cytogenetics 11(1): 1-13

DOI: 10.3897/CompCytogen.v11i1.9803

Authors: Jian-she Chen, Qi Gao, Hao Zhou, Yu-song Huang, Mikinori Ogisu, Ming Cao

Abstract: Aspidistra subrotata Y. Wan & C.C. Huang, 1987 is considered for the first time as a widespread polyploidy complex in the genus Aspidistra Ker Gawler, 1823 from limestone areas of Asia. The chromosome number of the tetraploid is 2n = 76 and the karyotype is formulated as 2n = 44 m + 8 sm + 24 st, while the chromosome number of the diploid is 2n = 38 and the karyotype formula 2n = 22 m + 4 sm + 12 st. In our studies, diploids occupy broader geographical and environmental niche spaces than tetraploids. Although the leaf-shape of A. subrotata varies quantitatively between and within diploid and/or tetraploid population(s), no obvious discontinuity in the width of leaf has been observed. The tetraploid plants may be distinguished from the diploid plants by their rigid petioles as well as thick deep green lamina. A. subrotata is therefore an interesting material to explore the formation and the evolutionary dynamics of a natural polyploid complex from limestone areas of the tropical regions.

HTML

XML

PDF

]]>
Research Article Mon, 9 Jan 2017 18:56:00 +0200
Karyological investigations and new chromosome number reports in Bellevalia Lapeyrouse, 1808 and Muscari Miller, 1758 (Asparagaceae) from Algeria https://compcytogen.pensoft.net/article/6445/ Comparative Cytogenetics 10(1): 171-187

DOI: 10.3897/CompCytogen.v10i1.6445

Authors: Nadjat Azizi, Rachid Amirouche, Nabila Amirouche

Abstract: Karyological investigations were carried out on four species of Bellevalia Lapeyrouse, 1808 and Muscari Miller, 1758 (Asparagaceae) sampled in contrasting bioclimatic conditions of Algeria. The endemic B. mauritanica Pomel, 1874 was found to have a tetraploid cytotype 2n = 4x = 16 and an octoploid 2n = 8x = 32 which is a new report. The chromosome number 2n = 2x = 18 in M. comosum (Linnaeus, 1753) Miller, 1768 and M. maritimum Desfontaines, 1798 was in conformity with earlier reports. The latter species reveals a lesser bimodality of the karyotype. Within M. neglectum Gussone ex Tenore, 1842 pentaploid (2n = 5x = 45), hexaploid (2n = 6x = 54) and very rare octoploid cytotype (2n = 8x = 72) have been reported in Algeria. Principal component analysis performed on basis of karyotype parameters, showed a segregation of the different cytotypes. This study provides new karyological information, which is discussed in a taxonomic context.

HTML

XML

PDF

]]>
Research Article Mon, 21 Mar 2016 11:13:01 +0200
Physical mapping of 5S and 18S ribosomal DNA in three species of Agave (Asparagales, Asparagaceae) https://compcytogen.pensoft.net/article/1798/ Comparative Cytogenetics 7(3): 191-203

DOI: 10.3897/compcytogen.v7i3.5337

Authors: Victor Manuel Gomez-Rodriguez, Benjamin Rodriguez-Garay, Guadalupe Palomino, Javier Martínez, Rodrigo Barba-Gonzalez

Abstract: Agave Linnaeus, 1753 is endemic of America and is considered one of the most important crops in Mexico due to its key role in the country’s economy. Cytogenetic analysis was carried out in A. tequilana Weber, 1902 ‘Azul’, A. cupreata Trelease et Berger, 1915 and A. angustifolia Haworth, 1812. The analysis showed that in all species the diploid chromosome number was 2n = 60, with bimodal karyotypes composed of five pairs of large chromosomes and 25 pairs of small chromosomes. Furthermore, different karyotypical formulae as well as a secondary constriction in a large chromosome pair were found in all species. Fluorescent in situ hybridization (FISH) was used for physical mapping of 5S and 18S ribosomal DNA (rDNA). All species analyzed showed that 5S rDNA was located in both arms of a small chromosome pair, while 18S rDNA was associated with the secondary constriction of a large chromosome pair. Data of FISH analysis provides new information about the position and number of rDNA loci and helps for detection of hybrids in breeding programs as well as evolutionary studies.

HTML

XML

PDF

]]>
Research Article Mon, 12 Aug 2013 00:00:00 +0300